MX9707666A - Proceso de seleccion de celulas de planta transgenica. - Google Patents

Proceso de seleccion de celulas de planta transgenica.

Info

Publication number
MX9707666A
MX9707666A MX9707666A MX9707666A MX9707666A MX 9707666 A MX9707666 A MX 9707666A MX 9707666 A MX9707666 A MX 9707666A MX 9707666 A MX9707666 A MX 9707666A MX 9707666 A MX9707666 A MX 9707666A
Authority
MX
Mexico
Prior art keywords
plant cells
transformed plant
transformed
proliferation
culture conditions
Prior art date
Application number
MX9707666A
Other languages
English (en)
Inventor
Anna Julia Trulson
Charles Edward Green
Carl Joseph Braun Iii
Original Assignee
Seminis Vegetables
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23654715&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX9707666(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Seminis Vegetables filed Critical Seminis Vegetables
Publication of MX9707666A publication Critical patent/MX9707666A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invencion comprende un proceso de seleccion para incrementar el numero de células de planta transformadas a partir de un mezcla de células de planta transformadas y no transformadas cultivadas bajo condiciones de cultivo heterotroficas. De acuerdo con este proceso, se cultiva una mezcla de células de planta transformadas y no transformadas bajo condiciones de cultivo heterotroficas en un medio de cultivo que contiene nutrientes minerales requeridos para la proliferacion y crecimiento de células de planta no transformadas excepto por una fuente de carbono que sustenta el crecimiento y la proliferacion, y aproximadamente 1.5 a 3 veces la cantidad estándar de fosforo. La fuente de carbono utilizada se sustituye por una fuente de carbono oculta que no sustenta el crecimiento y la proliferacion de dichas células no transformadas. Las células de planta transformadas tienen un segmento de ADN heterologo insertado dentro de su genoma, que contiene por lo menos un casete de expresion. El casete de expresion contiene un segmento marcador seleccionable de ADN heterologo que incluye un primer gen heterologo que codifica una enzima heterologa, que en la expresion convierte a la fuente de carbono oculta en una fuente de carbono que sustenta el crecimiento y la proliferacion de las células de planta transformadas bajo condiciones de cultivo heterotroficas. Adicionalmente, la presente invencion también contempla un proceso de seleccion para incrementar el numero de células de planta transformadas a partir de una mezcla de células de planta transformadas y no transformadas cultivadas bajo condiciones retardadas de cultivo selectivo.
MX9707666A 1995-04-06 1996-04-05 Proceso de seleccion de celulas de planta transgenica. MX9707666A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41761895A 1995-04-06 1995-04-06
PCT/US1996/004688 WO1996031612A2 (en) 1995-04-06 1996-04-05 Process for selection of transgenic plant cells

Publications (1)

Publication Number Publication Date
MX9707666A true MX9707666A (es) 1997-11-29

Family

ID=23654715

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9707666A MX9707666A (es) 1995-04-06 1996-04-05 Proceso de seleccion de celulas de planta transgenica.

Country Status (7)

Country Link
US (1) US6143562A (es)
EP (1) EP0820518B1 (es)
CN (1) CN1155713C (es)
AU (1) AU720006B2 (es)
ES (1) ES2256856T3 (es)
MX (1) MX9707666A (es)
WO (1) WO1996031612A2 (es)

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US8865468B2 (en) * 2009-10-19 2014-10-21 Aurora Algae, Inc. Homologous recombination in an algal nuclear genome
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Also Published As

Publication number Publication date
WO1996031612A3 (en) 1996-11-21
AU720006B2 (en) 2000-05-18
US6143562A (en) 2000-11-07
CN1155713C (zh) 2004-06-30
WO1996031612A2 (en) 1996-10-10
ES2256856T3 (es) 2006-07-16
AU5535196A (en) 1996-10-23
EP0820518B1 (en) 2005-12-07
EP0820518A2 (en) 1998-01-28
CN1187219A (zh) 1998-07-08

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