MX9707666A - Proceso de seleccion de celulas de planta transgenica. - Google Patents

Proceso de seleccion de celulas de planta transgenica.

Info

Publication number
MX9707666A
MX9707666A MX9707666A MX9707666A MX9707666A MX 9707666 A MX9707666 A MX 9707666A MX 9707666 A MX9707666 A MX 9707666A MX 9707666 A MX9707666 A MX 9707666A MX 9707666 A MX9707666 A MX 9707666A
Authority
MX
Mexico
Prior art keywords
plant cells
transformed plant
transformed
proliferation
culture conditions
Prior art date
Application number
MX9707666A
Other languages
English (en)
Inventor
Anna Julia Trulson
Charles Edward Green
Carl Joseph Braun Iii
Original Assignee
Seminis Vegetables
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23654715&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX9707666(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Seminis Vegetables filed Critical Seminis Vegetables
Publication of MX9707666A publication Critical patent/MX9707666A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La presente invencion comprende un proceso de seleccion para incrementar el numero de células de planta transformadas a partir de un mezcla de células de planta transformadas y no transformadas cultivadas bajo condiciones de cultivo heterotroficas. De acuerdo con este proceso, se cultiva una mezcla de células de planta transformadas y no transformadas bajo condiciones de cultivo heterotroficas en un medio de cultivo que contiene nutrientes minerales requeridos para la proliferacion y crecimiento de células de planta no transformadas excepto por una fuente de carbono que sustenta el crecimiento y la proliferacion, y aproximadamente 1.5 a 3 veces la cantidad estándar de fosforo. La fuente de carbono utilizada se sustituye por una fuente de carbono oculta que no sustenta el crecimiento y la proliferacion de dichas células no transformadas. Las células de planta transformadas tienen un segmento de ADN heterologo insertado dentro de su genoma, que contiene por lo menos un casete de expresion. El casete de expresion contiene un segmento marcador seleccionable de ADN heterologo que incluye un primer gen heterologo que codifica una enzima heterologa, que en la expresion convierte a la fuente de carbono oculta en una fuente de carbono que sustenta el crecimiento y la proliferacion de las células de planta transformadas bajo condiciones de cultivo heterotroficas. Adicionalmente, la presente invencion también contempla un proceso de seleccion para incrementar el numero de células de planta transformadas a partir de una mezcla de células de planta transformadas y no transformadas cultivadas bajo condiciones retardadas de cultivo selectivo.
MX9707666A 1995-04-06 1996-04-05 Proceso de seleccion de celulas de planta transgenica. MX9707666A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41761895A 1995-04-06 1995-04-06
PCT/US1996/004688 WO1996031612A2 (en) 1995-04-06 1996-04-05 Process for selection of transgenic plant cells

Publications (1)

Publication Number Publication Date
MX9707666A true MX9707666A (es) 1997-11-29

Family

ID=23654715

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9707666A MX9707666A (es) 1995-04-06 1996-04-05 Proceso de seleccion de celulas de planta transgenica.

Country Status (7)

Country Link
US (1) US6143562A (es)
EP (1) EP0820518B1 (es)
CN (1) CN1155713C (es)
AU (1) AU720006B2 (es)
ES (1) ES2256856T3 (es)
MX (1) MX9707666A (es)
WO (1) WO1996031612A2 (es)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9702592D0 (en) * 1997-02-07 1997-03-26 Danisco Selection method
EP0870836A1 (de) * 1997-04-09 1998-10-14 IPK Gatersleben 2-Desoxyglucose-6-Phosphat (2-DOG-6-P) Phosphatase DNA-Sequenzen als Selektionsmarker in Pflanzen
HUP9700762A3 (en) 1997-04-16 2000-08-28 Btg Int Ltd Transgenic stressresistant plants overexpressing iron-binding ferritin protein
HUP9702118A3 (en) * 1997-11-18 2000-08-28 Btg Int Ltd Plants over producing aldose reductase homologous protein can tolerate water deficiency and resist oxidative stresses, and process for producing them
GB9817465D0 (en) * 1998-08-11 1998-10-07 Danisco Selection method
EP1276885B1 (en) 2000-04-21 2007-09-12 Martek Biosciences Corporation Trophic conversion of obligate phototrophic algae through metabolic engineering
EP1780283A1 (en) * 2000-04-21 2007-05-02 Martek Biosciences Corporation Trophic conversion of obligate photographic algae through metabolic engineering
US7256050B2 (en) 2000-06-16 2007-08-14 Martek Biosciences Corp. High fluorescent intensity cross-linked allophycocyanin
EP1279737A1 (en) 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformation method for obtaining marker-free plants
NL1019308C2 (nl) * 2001-11-06 2003-05-07 Stichting Tech Wetenschapp Werkwijze voor het selecteren van genetisch getransformeerde cellen.
DK2069512T3 (da) * 2006-09-22 2010-09-20 Danisco Us Inc Genencor Div Acetolaktat-syntase fra Trichoderma reesei som selektionsmarkør
EP1949785A1 (en) 2007-01-26 2008-07-30 Coöperatie AVEBE U.A. Use of R-genes as a selection marker in plant transformation and use of cisgenes in plant transformation
WO2009149470A1 (en) 2008-06-06 2009-12-10 Aurora Biofuels, Inc. Vcp-based vectors for algal cell transformation
CA2781461C (en) * 2008-11-19 2023-01-17 Centro De Investigacion Y De Estudios Avanzados Del Instituto Politecnico Nacional_(Cinvestav) Transgenic plants and fungi capable of metabolizing phosphite as a source of phosphorus
US8314228B2 (en) * 2009-02-13 2012-11-20 Aurora Algae, Inc. Bidirectional promoters in Nannochloropsis
WO2010102293A1 (en) * 2009-03-06 2010-09-10 Metabolix, Inc. Method of positive plant selection using sorbitol dehydrogenase
MX2011009550A (es) * 2009-03-23 2011-10-12 Basf Plant Science Co Gmbh Plantas transgenicas con mecanismos redox alterados y mayor rendimiento.
US8865468B2 (en) * 2009-10-19 2014-10-21 Aurora Algae, Inc. Homologous recombination in an algal nuclear genome
US8809046B2 (en) 2011-04-28 2014-08-19 Aurora Algae, Inc. Algal elongases
US8865452B2 (en) * 2009-06-15 2014-10-21 Aurora Algae, Inc. Systems and methods for extracting lipids from wet algal biomass
US9101942B2 (en) * 2009-06-16 2015-08-11 Aurora Algae, Inc. Clarification of suspensions
US8747930B2 (en) * 2009-06-29 2014-06-10 Aurora Algae, Inc. Siliceous particles
US8404473B2 (en) 2009-06-30 2013-03-26 Aurora Algae, Inc. Cyanobacterial isolates having auto-flocculation and settling properties
WO2011011463A2 (en) * 2009-07-20 2011-01-27 Aurora Biofuels, Inc. Manipulation of an alternative respiratory pathway in photo-autotrophs
US8765983B2 (en) * 2009-10-30 2014-07-01 Aurora Algae, Inc. Systems and methods for extracting lipids from and dehydrating wet algal biomass
US8722359B2 (en) 2011-01-21 2014-05-13 Aurora Algae, Inc. Genes for enhanced lipid metabolism for accumulation of lipids
US8926844B2 (en) 2011-03-29 2015-01-06 Aurora Algae, Inc. Systems and methods for processing algae cultivation fluid
US8569530B2 (en) 2011-04-01 2013-10-29 Aurora Algae, Inc. Conversion of saponifiable lipids into fatty esters
AU2012249377A1 (en) 2011-04-28 2013-11-14 Aurora Algae, Inc. Algal desaturases
WO2013166065A1 (en) 2012-04-30 2013-11-07 Aurora Algae, Inc. ACP Promoter
US9266973B2 (en) 2013-03-15 2016-02-23 Aurora Algae, Inc. Systems and methods for utilizing and recovering chitosan to process biological material
CN114645070B (zh) * 2022-02-28 2022-12-20 北京焉支山科技有限公司 一种己糖-6-磷酸组合物的制备方法及其在化妆品中的应用

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1417505A (fr) * 1963-12-06 1965-11-12 Procédé pour la reproduction d'organismes vivants
US4038778A (en) * 1976-09-27 1977-08-02 Gte Laboratories Incorporated Tissue culture technique for asexual plant reproduction and a medium for use therewith
DE3270112D1 (en) * 1981-08-11 1986-04-30 Mitsui Petrochemical Ind Method for producing secondary metabolites of plants
US4473648A (en) * 1982-05-14 1984-09-25 International Plant Research Institute Process and nutrient medium for micropropagation of cassava
US5034322A (en) * 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US4634674A (en) * 1983-03-25 1987-01-06 Atlantic Richfield Company Plant regeneration from protoplasts
DE3504170A1 (de) * 1984-02-09 1985-08-22 Ecole Nationale d'Ingénieurs des Travaux Agricoles Option Horticulture, Angers, Maine-et-Loire Klon von crambe maritime und verfahren zu seiner vegetativen vermehrung
US4851346A (en) * 1984-04-19 1989-07-25 Miles Inc. Chemically defined growth medium
US4830966A (en) * 1984-09-07 1989-05-16 Sungene Technologies Corporation Process for regenerating corn
US4665030A (en) * 1984-09-07 1987-05-12 Sungene Technologies Corporation Process for regenerating corn
US4943674A (en) * 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US5188958A (en) * 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5066595A (en) * 1986-12-08 1991-11-19 Sungene Technologies Corporation Process for increasing free pool lysine content in maize
US5238841A (en) * 1987-04-06 1993-08-24 Cpc International Inc. Method for regulating plant growth
US5350689A (en) * 1987-05-20 1994-09-27 Ciba-Geigy Corporation Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
GB8826356D0 (en) * 1988-11-10 1988-12-14 Ici Plc Adp glucose-pyrophosphorylase
US5294549A (en) * 1989-03-09 1994-03-15 Weyerhaeuser Company Method for reproducing conifers by somatic embryogenesis using mixed growth hormones for embryo culture
US5041382A (en) * 1989-03-09 1991-08-20 Weyerhaeuser Company High concentration enrichment of conifer embryonal cells
US5236841A (en) * 1989-03-09 1993-08-17 Gupta Pramod K Method for reproducing conifers by somatic embryogenesis using stepwise hormone adjustment
DK198089D0 (da) * 1989-04-24 1989-04-24 Danske Spritfabrikker Dna-materialer og anvendelse deraf
US5302523A (en) * 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
AU644619B2 (en) * 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
DE4013144A1 (de) * 1990-04-20 1991-10-24 Inst Genbiologische Forschung Neue plasmide, enthaltend dna-sequenzen, die veraenderungen der karbohydrat- und proteinkonzentration und der karbohydrat- und proteinzusammensetzung in kartoffelknollen hervorrufen, sowie zellen einer kartoffelpflanze, enthaltend diese plasmide
US5349123A (en) * 1990-12-21 1994-09-20 Calgene, Inc. Glycogen biosynthetic enzymes in plants
DE69034128D1 (de) * 1990-07-20 2004-02-26 Bayer Cropscience Gmbh Saccharose-Phosphat-Synthase (SPS), ihr Verfahren zur Herstellung, ihr cDNA und Verwendung von cDNA zur Veränderung der SPS-Expression in Pflanzenzellen
DE4035756A1 (de) * 1990-11-08 1992-05-14 Inst Genbiologische Forschung Neue plasmide zur herstellung von im habitus und ertrag veraenderten transgenen pflanzen
SE467160B (sv) * 1990-12-21 1992-06-01 Amylogene Hb Genetisk foeraendring av potatis foer bildning av staerkelse av amylostyp
SE467358B (sv) * 1990-12-21 1992-07-06 Amylogene Hb Genteknisk foeraendring av potatis foer bildning av staerkelse av amylopektintyp
IL100908A0 (en) * 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use
GB9112645D0 (en) * 1991-06-12 1991-07-31 Cambridge Advanced Tech Modification of starch production
GB9117159D0 (en) * 1991-08-08 1991-09-25 Cambridge Advanced Tech Modification of sucrose accumulation
GB9304200D0 (en) * 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
DK152291D0 (da) * 1991-08-28 1991-08-28 Danisco Fremgangsmaade og kemiske forbindelser
GB9218185D0 (en) * 1992-08-26 1992-10-14 Ici Plc Novel plants and processes for obtaining them
ES2217254T3 (es) * 1992-10-14 2004-11-01 Syngenta Limited Nuevas plantas y procesos para obtenerlas.
GB9223454D0 (en) * 1992-11-09 1992-12-23 Ici Plc Novel plants and processes for obtaining them

Also Published As

Publication number Publication date
AU720006B2 (en) 2000-05-18
CN1187219A (zh) 1998-07-08
WO1996031612A2 (en) 1996-10-10
EP0820518A2 (en) 1998-01-28
CN1155713C (zh) 2004-06-30
ES2256856T3 (es) 2006-07-16
EP0820518B1 (en) 2005-12-07
AU5535196A (en) 1996-10-23
US6143562A (en) 2000-11-07
WO1996031612A3 (en) 1996-11-21

Similar Documents

Publication Publication Date Title
MX9707666A (es) Proceso de seleccion de celulas de planta transgenica.
WO1991002071A3 (en) Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
WO1995006128A3 (en) Fertile, transgenic maize plants and methods for their production
EP0672160B8 (en) Activating expression of an amplifying endogenous gene by homologous recombination
SG173213A1 (en) Identification of genes
TW200504213A (en) Method of preparing amino acids by fermentation with the constructed amino acid producing bacteria
EP0805851A4 (en) BINARY BAC VECTOR
RU97106101A (ru) Экспрессия промотора синтазы ацето-оксикислот в интродуцированных генах растений
GR3034722T3 (en) ENHANCED PRODUCTION OF TAXOL AND TAXANES BY CELL CULTURES OF -i(TAXUS) SPECIES.
IL97645A (en) Production of enzymes in seeds and their use
MY133278A (en) Method of producing l-lysine by fermentation
CA2257198A1 (en) Suppression of specific classes of soybean seed protein genes
CA2264270A1 (en) Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture
HUP0500499A2 (hu) Eljárás növényekben a szervek tömegének megváltoztatására, a termékenység ellenőrzésére és az aszexuális reprodukció fokozására
HU0302357D0 (en) Method for production of taxanes by cell cultures of taxus species
AU1156797A (en) Commercial production of aprotinin in plants
HUP9901292A2 (hu) Eljárás növényi anyag szaporítására és/vagy szelektálására
FI20095510A (fi) Suuren solutiheyden bakteerifermentaatioviljelmä
HU9201218D0 (en) Process for the entering of non-auxotrophic bacteries -belonging to the azotobacteraceae family - into the intercellular spaces of plant tissues, for their cultivation in vitro, with the aim to create new nitrogenfixing plant-bacterium symbiosis
DE3071292D1 (en) Recombinant bacteriophage for heterologous cloning of bacillus microorganisms and method for its production
EP2298911A3 (en) Methods for producing polypeptides in respiratory-deficient cells
CN111778274A (zh) 一种通过基因编辑提高番茄耐贮藏性的方法
Tolley et al. Assignment to chromosome 16 of a gene necessary for the expression of human mitochondrial glutamate oxaloacetate transaminase (aspartate aminotransferase)(EC 2.6. 1.1.)
Fukuoka et al. Molecular changes of organelle DNA sequences in rice through dedifferentiation, long-term culture, or the morphogenesis process
CA2064761A1 (en) Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof