CA2064761A1 - Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof - Google Patents
Methods and compositions for the production of stably transformed fertile monocot plants and cells thereofInfo
- Publication number
- CA2064761A1 CA2064761A1 CA002064761A CA2064761A CA2064761A1 CA 2064761 A1 CA2064761 A1 CA 2064761A1 CA 002064761 A CA002064761 A CA 002064761A CA 2064761 A CA2064761 A CA 2064761A CA 2064761 A1 CA2064761 A1 CA 2064761A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- methods
- transformed
- production
- compositions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 6
- 241000209510 Liliopsida Species 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 241000196324 Embryophyta Species 0.000 abstract 2
- 240000008042 Zea mays Species 0.000 abstract 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 2
- 235000009973 maize Nutrition 0.000 abstract 2
- 241000187747 Streptomyces Species 0.000 abstract 1
- 101150103518 bar gene Proteins 0.000 abstract 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 abstract 1
- 238000012136 culture method Methods 0.000 abstract 1
- 230000000408 embryogenic effect Effects 0.000 abstract 1
- 230000002363 herbicidal effect Effects 0.000 abstract 1
- 239000004009 herbicide Substances 0.000 abstract 1
- 238000004114 suspension culture Methods 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39217689A | 1989-08-09 | 1989-08-09 | |
| US392,176 | 1989-08-09 | ||
| US51329890A | 1990-04-17 | 1990-04-17 | |
| US513,298 | 1990-04-17 | ||
| PCT/US1990/004462 WO1991002071A2 (en) | 1989-08-09 | 1990-08-08 | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2064761A1 true CA2064761A1 (en) | 1991-02-10 |
| CA2064761C CA2064761C (en) | 2006-06-13 |
Family
ID=36587326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002064761A Expired - Lifetime CA2064761C (en) | 1989-08-09 | 1990-08-08 | Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2064761C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6326527B1 (en) | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
| US6329574B1 (en) | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
| US6399861B1 (en) | 1990-04-17 | 2002-06-04 | Dekalb Genetics Corp. | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6777589B1 (en) | 1990-01-22 | 2004-08-17 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6803499B1 (en) | 1989-08-09 | 2004-10-12 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
-
1990
- 1990-08-08 CA CA002064761A patent/CA2064761C/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6803499B1 (en) | 1989-08-09 | 2004-10-12 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6329574B1 (en) | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
| US6777589B1 (en) | 1990-01-22 | 2004-08-17 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6399861B1 (en) | 1990-04-17 | 2002-06-04 | Dekalb Genetics Corp. | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6326527B1 (en) | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2064761C (en) | 2006-06-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |