BR9706843A - Enzima de xilanase, xilanase modificada e processos para melhorar a capacidade de branqueamento de pasta de madeira e para modificar um enzima de xilanase - Google Patents

Enzima de xilanase, xilanase modificada e processos para melhorar a capacidade de branqueamento de pasta de madeira e para modificar um enzima de xilanase

Info

Publication number
BR9706843A
BR9706843A BR9706843-8A BR9706843A BR9706843A BR 9706843 A BR9706843 A BR 9706843A BR 9706843 A BR9706843 A BR 9706843A BR 9706843 A BR9706843 A BR 9706843A
Authority
BR
Brazil
Prior art keywords
xylanase
amino acids
enzyme
modified
processes
Prior art date
Application number
BR9706843-8A
Other languages
English (en)
Inventor
Wing L Sung
Makoto Yaguchi
Kazuhiko Ishikawa
Original Assignee
Nat Reserch Council Of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Reserch Council Of Canada filed Critical Nat Reserch Council Of Canada
Publication of BR9706843A publication Critical patent/BR9706843A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Paper (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"ENZIMA DE XILANASE, XILANASE MODIFICADA E PROCESSOS PARA MELHORAR A CAPACIDADE DE BRANQUEAMENTO DE PASTA DE MADEIRA E PARA MODIFICAR UMA ENZIMA DE XILANASE". Produção de uma enzima de xilanase de desempenho superior no branqueamento da pasta. Mais especificamente, uma xilanase modificada da Família 11 que mostra melhoradas termofilicidade, alcalofilicidade e termoestabilidade quando comparada à xilanase natural. As xilanases modificadas contêm qualquer um de três tipos de modificações: (1) mudar aminoácidos 10, 27 e 29 da xilanase de Trichoderma reesei II ou os correspondentes aminoácidos de outra xilanase da Família 11, em que estes aminoácidos são mudados para histidina, metionina e leucina, respectivamente; (2) substituição de aminoácidos na região N-terminal com aminoácidos de outra enzima de xilanase. Em uma forma de realização preferida, a substituição da xilanase de Bacillus circulans ou Trichoderma reesei natural com uma sequência curta de aminoácidos da xilanase de Thermomonospora fusca deu xilanases quiméricas com termofilicidade e alcalofilicidade mais altas; (3) uma extensão a montante do N-término de até 10 aminoácidos. Em uma forma de realização preferida, a extensão do N-término da xilanase com o tripeptídeo glicina-arginina-arginina melhorou seu desempenho.
BR9706843-8A 1996-09-09 1997-11-07 Enzima de xilanase, xilanase modificada e processos para melhorar a capacidade de branqueamento de pasta de madeira e para modificar um enzima de xilanase BR9706843A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/709,912 US5759840A (en) 1996-09-09 1996-09-09 Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability

Publications (1)

Publication Number Publication Date
BR9706843A true BR9706843A (pt) 2004-03-23

Family

ID=24851808

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9706843-8A BR9706843A (pt) 1996-09-09 1997-11-07 Enzima de xilanase, xilanase modificada e processos para melhorar a capacidade de branqueamento de pasta de madeira e para modificar um enzima de xilanase

Country Status (9)

Country Link
US (2) US5759840A (pt)
EP (1) EP0828002B1 (pt)
JP (1) JPH10179155A (pt)
CN (2) CN1155700C (pt)
AR (1) AR009574A1 (pt)
BR (1) BR9706843A (pt)
CA (1) CA2210247C (pt)
DE (1) DE69712003D1 (pt)
NZ (1) NZ328680A (pt)

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US7718411B1 (en) 2004-08-05 2010-05-18 Danisco Us Inc. Trichoderma reesei G/11 xylanases with improved stability
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CN100354424C (zh) * 2003-11-17 2007-12-12 中国农业大学 一种耐高温木聚糖酶的表达方法及其专用表达载体
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ES2817574T3 (es) 2011-11-25 2021-04-07 Mitsui Chemicals Inc Xilanasa mutante, método de fabricación y uso de la misma, y método para fabricar lignocelulosa sacarificada
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CN103184204A (zh) * 2011-12-29 2013-07-03 中国科学院微生物研究所 木聚糖酶SoxB及其编码基因
CN103642774B (zh) * 2013-11-13 2016-08-17 宁夏夏盛实业集团有限公司 混合中性纤维素酶及其制备方法和在造纸打浆中的应用
CN103627686B (zh) * 2013-11-14 2015-03-18 青岛蔚蓝生物集团有限公司 一种木聚糖酶突变体及其应用
JP2015167552A (ja) * 2014-03-11 2015-09-28 花王株式会社 変異キシラナーゼ
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JP2016029908A (ja) 2014-07-28 2016-03-07 本田技研工業株式会社 Ｇｈファミリー１０に属する耐熱性キシラナーゼ
JP6354462B2 (ja) 2014-08-29 2018-07-11 本田技研工業株式会社 Ｇｈファミリー１０に属する耐熱性キシラナーゼ
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JP7135334B2 (ja) * 2018-02-22 2022-09-13 株式会社豊田中央研究所 キシラナーゼ及びその利用
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Also Published As

Publication number Publication date
JPH10179155A (ja) 1998-07-07
CN1550548A (zh) 2004-12-01
EP0828002B1 (en) 2002-04-17
DE69712003D1 (de) 2002-05-23
US5866408A (en) 1999-02-02
NZ328680A (en) 1998-04-27
CN1155700C (zh) 2004-06-30
CN1177639A (zh) 1998-04-01
CA2210247A1 (en) 1998-03-09
AR009574A1 (es) 2000-04-26
EP0828002A2 (en) 1998-03-11
US5759840A (en) 1998-06-02
CN100595276C (zh) 2010-03-24
EP0828002A3 (en) 1998-09-16
CA2210247C (en) 2002-11-26

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Legal Events

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B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements