DE69712003D1 - Modifikation von xylanase zur Verbesserung von Thermophilicität, alkophilicität und thermostabilität - Google Patents

Modifikation von xylanase zur Verbesserung von Thermophilicität, alkophilicität und thermostabilität

Info

Publication number
DE69712003D1
DE69712003D1 DE69712003T DE69712003T DE69712003D1 DE 69712003 D1 DE69712003 D1 DE 69712003D1 DE 69712003 T DE69712003 T DE 69712003T DE 69712003 T DE69712003 T DE 69712003T DE 69712003 D1 DE69712003 D1 DE 69712003D1
Authority
DE
Germany
Prior art keywords
alkophilicity
thermophilicity
xylanase
thermostability
modification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69712003T
Other languages
English (en)
Inventor
Wing L Sung
Makoto Yaguchi
Kazuhiko Ishikawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
National Research Council of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Council of Canada filed Critical National Research Council of Canada
Application granted granted Critical
Publication of DE69712003D1 publication Critical patent/DE69712003D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Paper (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69712003T 1996-09-09 1997-09-05 Modifikation von xylanase zur Verbesserung von Thermophilicität, alkophilicität und thermostabilität Expired - Lifetime DE69712003D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/709,912 US5759840A (en) 1996-09-09 1996-09-09 Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability

Publications (1)

Publication Number Publication Date
DE69712003D1 true DE69712003D1 (de) 2002-05-23

Family

ID=24851808

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69712003T Expired - Lifetime DE69712003D1 (de) 1996-09-09 1997-09-05 Modifikation von xylanase zur Verbesserung von Thermophilicität, alkophilicität und thermostabilität

Country Status (9)

Country Link
US (2) US5759840A (de)
EP (1) EP0828002B1 (de)
JP (1) JPH10179155A (de)
CN (2) CN1155700C (de)
AR (1) AR009574A1 (de)
BR (1) BR9706843A (de)
CA (1) CA2210247C (de)
DE (1) DE69712003D1 (de)
NZ (1) NZ328680A (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
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US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability
US5871550A (en) * 1997-08-26 1999-02-16 Genencor International, Inc. Mutant Thermonospora spp. cellulase
DE69933939T3 (de) * 1998-11-16 2012-03-01 National Research Council Of Canada Hitzestabile xylanasen
US7060482B1 (en) * 1998-11-16 2006-06-13 National Research Council Of Canada Thermostable xylanases
US6329186B1 (en) 1998-12-07 2001-12-11 Novozymes A/S Glucoamylases with N-terminal extensions
WO2000034452A1 (en) * 1998-12-07 2000-06-15 Novozymes A/S Glucoamylases with n-terminal extensions
BRPI9916507B1 (pt) * 1998-12-23 2015-09-08 Danisco uso de xilanase bacteriana para a produção de um produto de panificação ou massa para preparação do mesmo e uso de uma sequência de aminoácidos
US20050079573A1 (en) 1998-12-23 2005-04-14 Danisco A/S Proteins
US6682923B1 (en) 1999-05-12 2004-01-27 Xencor Thermostable alkaliphilic xylanase
EP1179075A2 (de) * 1999-05-12 2002-02-13 Xencor, Inc. Bacillus circulans xylanase mutante
FI108728B (fi) * 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
US7718411B1 (en) 2004-08-05 2010-05-18 Danisco Us Inc. Trichoderma reesei G/11 xylanases with improved stability
DE60017942T2 (de) 1999-12-30 2006-05-18 Genencor International, Inc., Palo Alto Trichoderma reesei xylanase
AU785107C (en) * 2000-03-08 2007-05-03 Dupont Nutrition Biosciences Aps Xylanase variants having altered sensitivity to xylanase inhibitors
CA2410917C (en) * 2000-05-31 2012-11-27 Wing L. Sung Modified xylanases exhibiting increased thermophilicity and alkalophilicity
AU2002230784A1 (en) * 2000-11-10 2002-05-21 Xencor Novel thermostable alkaliphilic xylanase
AU2002226210A1 (en) * 2000-12-22 2002-07-08 Iogen Bio-Products Corporation Alkaline extraction stages comprising xylanase
US7320741B2 (en) * 2001-01-18 2008-01-22 Iogen Bio-Products Corporation Method of xylanase treatment in a chlorine dioxide bleaching sequence
US7510860B1 (en) * 2001-11-21 2009-03-31 National Research Council Of Canada Xylanases with enhanced thermophilicity and alkalophilicity
AUPR968801A0 (en) * 2001-12-21 2002-01-24 Unisearch Limited Improvements in enzyme stability
CN100346030C (zh) * 2002-03-06 2007-10-31 埃欧金生物制品公司 化学纸浆的木聚糖酶处理
EP2314698A1 (de) 2002-06-14 2011-04-27 Verenium Corporation Xylanasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
US20040112555A1 (en) * 2002-12-03 2004-06-17 Jeffrey Tolan Bleaching stage using xylanase with hydrogen peroxide, peracids, or a combination thereof
EP2404929B1 (de) 2003-07-02 2014-06-18 BP Corporation North America Inc. Glucanasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
US7741089B2 (en) 2003-08-11 2010-06-22 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
US20070243595A1 (en) * 2006-04-14 2007-10-18 Clarkson Kathleen A Modified enzymes, methods to produce modified enzymes and uses thereof
WO2005108565A2 (en) * 2003-09-15 2005-11-17 Genencor International, Inc. Modified xylanase, methods of making and use thereof
CN100354424C (zh) * 2003-11-17 2007-12-12 中国农业大学 一种耐高温木聚糖酶的表达方法及其专用表达载体
CN101023173B (zh) * 2004-03-25 2012-08-08 埃欧金生物制品公司 呈现出增加表达的修饰型木聚糖酶
CN100491533C (zh) * 2005-04-13 2009-05-27 中国农业科学院饲料研究所 改良的高比活木聚糖酶及其基因、包括该基因的表达载体和重组酵母细胞以及表达方法
EP2548955A1 (de) 2006-02-14 2013-01-23 Verenium Corporation Xylanasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
US7723712B2 (en) * 2006-03-17 2010-05-25 Micron Technology, Inc. Reduced power consumption phase change memory and methods for forming the same
RU2464313C2 (ru) * 2006-04-12 2012-10-20 Нэйшенл Рисерч Каунсил Оф Кэнэда Модифицированная ксиланаза
WO2007115391A1 (en) * 2006-04-12 2007-10-18 National Research Council Of Cananda Modification of xylanases to increase thermophilicity, thermostability and alkalophilicity
CN101466879B (zh) 2006-06-08 2011-08-17 加拿大国立研究院 大麻纤维的提取
BRPI0714876B1 (pt) 2006-08-04 2022-04-19 Verenium Corporation Ácido nucleico isolado, sintético ou recombinante, cassete de expressão, vetor ou veículo de clonagem, célula bacteriana, fúngica ou de levedura transformada, polipeptídeo isolado, sintético ou recombinante, composição, bem como métodos de produção e de usos dos mesmos
CN100419069C (zh) * 2006-08-29 2008-09-17 湖北大学 一种耐热耐碱木聚糖酶酵母工程菌及其耐热木聚糖酶的生产方法
CA2663819C (en) 2006-09-21 2019-04-30 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
CN101289668B (zh) * 2007-04-16 2010-09-08 屏东科技大学 高酸碱反应范围的聚木醣酶的突变基因及其点突变方法
WO2009045627A2 (en) 2007-10-03 2009-04-09 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
MX2011002142A (es) * 2008-08-29 2011-04-26 Iogen Energy Corp Beta-glucosidasas modificadas con estabilidad mejorada.
KR101221003B1 (ko) 2008-12-12 2013-01-10 한국생명공학연구원 미생물의 열안정성 및 산 저항성을 조절하는 방법
CN106011159A (zh) 2009-05-21 2016-10-12 巴斯夫酶有限责任公司 肌醇六磷酸酶、编码它们的核酸及制备和使用它们的方法
CA2791353A1 (en) * 2010-03-03 2011-09-09 Novozymes, Inc. Xylanase variants and polynucleotides encoding same
EP2784161B1 (de) 2011-11-25 2018-06-20 Mitsui Chemicals, Inc. Mutierte xylanase, herstellungsverfahren dafür und verwendung davon sowie verfahren zur herstellung saccharifizierter lignocellulose
CN103184204A (zh) * 2011-12-29 2013-07-03 中国科学院微生物研究所 木聚糖酶SoxB及其编码基因
CN103184205A (zh) * 2011-12-29 2013-07-03 中国科学院微生物研究所 木聚糖酶SlxB及其编码基因
CN103642774B (zh) * 2013-11-13 2016-08-17 宁夏夏盛实业集团有限公司 混合中性纤维素酶及其制备方法和在造纸打浆中的应用
CN103627686B (zh) * 2013-11-14 2015-03-18 青岛蔚蓝生物集团有限公司 一种木聚糖酶突变体及其应用
JP2015167552A (ja) * 2014-03-11 2015-09-28 花王株式会社 変異キシラナーゼ
US10385326B2 (en) 2014-05-30 2019-08-20 Novozymes A/S Variants of GH family 11 xylanase and polynucleotides encoding same
JP2016029908A (ja) 2014-07-28 2016-03-07 本田技研工業株式会社 Ｇｈファミリー１０に属する耐熱性キシラナーゼ
JP6354462B2 (ja) 2014-08-29 2018-07-11 本田技研工業株式会社 Ｇｈファミリー１０に属する耐熱性キシラナーゼ
WO2016073610A1 (en) 2014-11-07 2016-05-12 Novozymes A/S Xylanase based bleach boosting
JP7135334B2 (ja) * 2018-02-22 2022-09-13 株式会社豊田中央研究所 キシラナーゼ及びその利用
CN114438057B (zh) * 2022-03-16 2023-05-19 齐鲁工业大学 一种耐热耐碱木聚糖酶及应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114807093B (zh) * 2022-06-22 2022-09-27 中国农业科学院北京畜牧兽医研究所 一种通过c端添加融合肽段提高木聚糖酶和植酸酶热稳定性的方法
CN116064479B (zh) * 2022-11-30 2024-04-09 山东龙昌动物保健品股份有限公司 一种含木聚糖酶突变体和胆汁酸的复合制剂及其应用

Family Cites Families (5)

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GB9018426D0 (en) * 1990-08-22 1990-10-03 Sandoz Ltd Improvements in or relating to novel compounds
DE4226528A1 (de) * 1992-08-11 1994-02-17 Roehm Gmbh Batterien-Xylanase, Verfahren zu ihrer Herstellung sowie dafür geeigneter Bakterienstamm, Plasmid mit zugehörigem Strukturgen, sowie Backmittel und Backverfahren zur Herstellung von Brot und Backwaren unter Verwendung der Xylanase
US5405769A (en) * 1993-04-08 1995-04-11 National Research Council Of Canada Construction of thermostable mutants of a low molecular mass xylanase
WO1995012668A1 (en) * 1993-11-05 1995-05-11 Cornell Research Foundation, Inc. Thermostable xylanase from a thermomonospora fusca gene
US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability

Also Published As

Publication number Publication date
CN1177639A (zh) 1998-04-01
CA2210247C (en) 2002-11-26
JPH10179155A (ja) 1998-07-07
BR9706843A (pt) 2004-03-23
CN100595276C (zh) 2010-03-24
EP0828002A3 (de) 1998-09-16
CA2210247A1 (en) 1998-03-09
NZ328680A (en) 1998-04-27
EP0828002B1 (de) 2002-04-17
US5759840A (en) 1998-06-02
CN1550548A (zh) 2004-12-01
US5866408A (en) 1999-02-02
CN1155700C (zh) 2004-06-30
EP0828002A2 (de) 1998-03-11
AR009574A1 (es) 2000-04-26

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