BR112023019898A2 - Métodos aprimorados de preparação de biblioteca - Google Patents

Métodos aprimorados de preparação de biblioteca

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Publication number
BR112023019898A2
BR112023019898A2 BR112023019898A BR112023019898A BR112023019898A2 BR 112023019898 A2 BR112023019898 A2 BR 112023019898A2 BR 112023019898 A BR112023019898 A BR 112023019898A BR 112023019898 A BR112023019898 A BR 112023019898A BR 112023019898 A2 BR112023019898 A2 BR 112023019898A2
Authority
BR
Brazil
Prior art keywords
preparation methods
library preparation
modified
end sequence
improved library
Prior art date
Application number
BR112023019898A
Other languages
English (en)
Inventor
Allison Yunghans
Marie Barr Schalembier Angelica
Kayla Busby
m gross Stephen
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of BR112023019898A2 publication Critical patent/BR112023019898A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Abstract

métodos aprimorados de preparação de biblioteca. é aqui divulgada uma sequência de extremidade de transposon modificada compreendendo uma sequência de extremidade em mosaico, em que a sequência de extremidade em mosaico compreende uma ou mais mutações em comparação com uma sequência de extremidade em mosaico de tipo selvagem, em que a mutação compreende uma substituição por uma uracila, uma inosina, uma ribose, uma 8-oxoguanina, uma timina glicol, uma purina modificada ou uma pirimidina modificada. -também são divulgados complexos de transpossomo compreendendo estas sequências de extremidades de transposon modificadas e métodos de preparação de biblioteca usando estas sequências de extremidades de transposon modificadas.
BR112023019898A 2021-03-29 2022-03-28 Métodos aprimorados de preparação de biblioteca BR112023019898A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163167150P 2021-03-29 2021-03-29
US202163224201P 2021-07-21 2021-07-21
PCT/US2022/022167 WO2022212269A1 (en) 2021-03-29 2022-03-28 Improved methods of library preparation

Publications (1)

Publication Number Publication Date
BR112023019898A2 true BR112023019898A2 (pt) 2023-11-07

Family

ID=81346345

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023019898A BR112023019898A2 (pt) 2021-03-29 2022-03-28 Métodos aprimorados de preparação de biblioteca

Country Status (8)

Country Link
EP (1) EP4314279A1 (pt)
JP (1) JP2024511766A (pt)
KR (1) KR20230161979A (pt)
AU (1) AU2022252197A1 (pt)
BR (1) BR112023019898A2 (pt)
CA (1) CA3214278A1 (pt)
IL (1) IL307195A (pt)
WO (1) WO2022212269A1 (pt)

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US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
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Also Published As

Publication number Publication date
EP4314279A1 (en) 2024-02-07
KR20230161979A (ko) 2023-11-28
AU2022252197A1 (en) 2023-09-21
WO2022212269A1 (en) 2022-10-06
CA3214278A1 (en) 2022-10-06
IL307195A (en) 2023-11-01
JP2024511766A (ja) 2024-03-15

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