BR112022005392A2 - Caracterização de partículas virais de terapia genética que usa cromatografia por exclusão de tamanho e tecnologias de dispersão de luz multiangular - Google Patents

Caracterização de partículas virais de terapia genética que usa cromatografia por exclusão de tamanho e tecnologias de dispersão de luz multiangular

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Publication number
BR112022005392A2
BR112022005392A2 BR112022005392A BR112022005392A BR112022005392A2 BR 112022005392 A2 BR112022005392 A2 BR 112022005392A2 BR 112022005392 A BR112022005392 A BR 112022005392A BR 112022005392 A BR112022005392 A BR 112022005392A BR 112022005392 A2 BR112022005392 A2 BR 112022005392A2
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Brazil
Prior art keywords
viral particles
size exclusion
exclusion chromatography
light scattering
characterization
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BR112022005392A
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English (en)
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Bhat Vikas
Yehuda Berguig Geoffrey
Louise Mcintosh Nicole
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Biomarin Pharm Inc
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Publication date
Application filed by Biomarin Pharm Inc filed Critical Biomarin Pharm Inc
Publication of BR112022005392A2 publication Critical patent/BR112022005392A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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  • Life Sciences & Earth Sciences (AREA)
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Abstract

CARACTERIZAÇÃO DE PARTÍCULAS VIRAIS DE TERAPIA GENÉTICA QUE USA CROMATOGRAFIA POR EXCLUSÃO DE TAMANHO E TECNOLOGIAS DE DISPERSÃO DE LUZ MULTIANGULA. Esta divulgação refere-se ao uso de cromatografia por exclusão de tamanho e/ou cromatografia por exclusão de tamanho com tecnologia de dispersão de luz multiangular para caracterizar partículas virais, tais como vírus adenoassociado e partículas de lentivírus. Os métodos divulgados também são úteis para estimar o título de partículas virais, determinar a integridade das partículas virais e estimar a quantidade de DNA encapsulado na partícula viral.
BR112022005392A 2019-09-27 2020-09-25 Caracterização de partículas virais de terapia genética que usa cromatografia por exclusão de tamanho e tecnologias de dispersão de luz multiangular BR112022005392A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962907509P 2019-09-27 2019-09-27
US202063043571P 2020-06-24 2020-06-24
PCT/US2020/052738 WO2021062164A1 (en) 2019-09-27 2020-09-25 Characterization of gene therapy viral particles using size exclusion chromatography and multi-angle light scattering technologies

Publications (1)

Publication Number Publication Date
BR112022005392A2 true BR112022005392A2 (pt) 2022-09-06

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Country Status (11)

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US (1) US20220308022A1 (pt)
EP (1) EP4034642A1 (pt)
JP (1) JP2022549679A (pt)
KR (1) KR20220066164A (pt)
CN (1) CN114729333A (pt)
AU (1) AU2020354669A1 (pt)
BR (1) BR112022005392A2 (pt)
CA (1) CA3153782A1 (pt)
IL (1) IL291101A (pt)
MX (1) MX2022003681A (pt)
WO (1) WO2021062164A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021236908A2 (en) 2020-05-20 2021-11-25 Biomarin Pharmaceutical Inc. Use of regulatory proteins for the production of adeno-associated virus
US20210396642A1 (en) * 2020-06-18 2021-12-23 Wyatt Technology Corporation Calculating molar mass values of components of and molar mass concentration values of conjugate molecules/particles
AU2021372262A1 (en) 2020-11-02 2023-06-01 Biomarin Pharmaceutical Inc. Process for enriching adeno-associated virus
WO2023034994A1 (en) 2021-09-03 2023-03-09 Biomarin Pharmaceutical Inc. Aav capsid compositions and methods for delivery
WO2023034990A1 (en) 2021-09-03 2023-03-09 Biomarin Pharmaceutical Inc. Aav capsid compositions and methods for delivery
WO2023034980A1 (en) 2021-09-03 2023-03-09 Bomarin Pharmaceutical Inc. Aav capsid compositions and methods for delivery
WO2023034997A1 (en) 2021-09-03 2023-03-09 Biomarin Pharmaceutical Inc. Aav capsid compositions and methods for delivery
WO2023034996A1 (en) 2021-09-03 2023-03-09 Biomarin Pharmaceutical Inc. Aav capsid compositions and methods for delivery
WO2023034989A1 (en) 2021-09-03 2023-03-09 Biomarin Pharmaceutical Inc. Aav capsid compositions and methods for delivery
WO2023056436A2 (en) 2021-10-01 2023-04-06 Biomarin Pharmaceutical Inc. Treatment of hereditary angioedema with aav gene therapy vectors and therapeutic formulations
WO2024054313A1 (en) * 2022-09-07 2024-03-14 Wyatt Technology, Llc Measuring quality attributes of a virus sample

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US4745051A (en) 1983-05-27 1988-05-17 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
EP0127839B1 (en) 1983-05-27 1992-07-15 THE TEXAS A&M UNIVERSITY SYSTEM Method for producing a recombinant baculovirus expression vector
ZA848495B (en) 1984-01-31 1985-09-25 Idaho Res Found Production of polypeptides in insect cells
US6204059B1 (en) 1994-06-30 2001-03-20 University Of Pittsburgh AAV capsid vehicles for molecular transfer
WO1998010088A1 (en) 1996-09-06 1998-03-12 Trustees Of The University Of Pennsylvania An inducible method for production of recombinant adeno-associated viruses utilizing t7 polymerase
US6723551B2 (en) 2001-11-09 2004-04-20 The United States Of America As Represented By The Department Of Health And Human Services Production of adeno-associated virus in insect cells
AU2003212708A1 (en) 2002-03-05 2003-09-16 Stichting Voor De Technische Wetenschappen Baculovirus expression system
US7943379B2 (en) 2008-04-30 2011-05-17 Nationwide Children's Hospital, Inc. Production of rAAV in vero cells using particular adenovirus helpers
EP3992283A1 (en) * 2015-12-11 2022-05-04 The Trustees Of The University Of Pennsylvania Scalable purification method for aavrh10

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Publication number Publication date
US20220308022A1 (en) 2022-09-29
MX2022003681A (es) 2022-05-10
WO2021062164A1 (en) 2021-04-01
CA3153782A1 (en) 2022-03-08
EP4034642A1 (en) 2022-08-03
CN114729333A (zh) 2022-07-08
JP2022549679A (ja) 2022-11-28
WO2021062164A9 (en) 2021-05-14
AU2020354669A1 (en) 2022-04-07
KR20220066164A (ko) 2022-05-23
IL291101A (en) 2022-05-01

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