BR112021026266A2 - Levedura modificada e método para aumentar o teor de lisina em coprodutos de fermentação - Google Patents

Levedura modificada e método para aumentar o teor de lisina em coprodutos de fermentação

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Publication number
BR112021026266A2
BR112021026266A2 BR112021026266A BR112021026266A BR112021026266A2 BR 112021026266 A2 BR112021026266 A2 BR 112021026266A2 BR 112021026266 A BR112021026266 A BR 112021026266A BR 112021026266 A BR112021026266 A BR 112021026266A BR 112021026266 A2 BR112021026266 A2 BR 112021026266A2
Authority
BR
Brazil
Prior art keywords
products
fermentation
lysine
modified yeast
lysine content
Prior art date
Application number
BR112021026266A
Other languages
English (en)
Inventor
Emily Gaby Payen Celia
Diana Demartini Jaclyn
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of BR112021026266A2 publication Critical patent/BR112021026266A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03014—Homocitrate synthase (2.3.3.14)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

levedura modificada e método para aumentar o teor de lisina em coprodutos de fermentação. a presente invenção refere-se a cepas e métodos relacionados com células de levedura geneticamente modificadas que superproduzem lisina de uma maneira ajustável alterando-se a inibição de retroalimentação da trajetória sintética de lisina por meio dos polipeptídeos homocitrato sintase lys20 e lys21. a levedura pode ser usada em uma instalação de produção de bioetanol convencional para produzir álcool juntamente com quantidades aumentadas de lisina, resultando em qualidade e valor comercial aumentados de produtos e coprodutos de fermentação, tais como ingredientes de ração para animal.
BR112021026266A 2019-06-24 2020-06-22 Levedura modificada e método para aumentar o teor de lisina em coprodutos de fermentação BR112021026266A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962865457P 2019-06-24 2019-06-24
PCT/US2020/038879 WO2020263720A1 (en) 2019-06-24 2020-06-22 Modified yeast and method for increasing lysine content in fermentation co-products

Publications (1)

Publication Number Publication Date
BR112021026266A2 true BR112021026266A2 (pt) 2022-05-31

Family

ID=71528067

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021026266A BR112021026266A2 (pt) 2019-06-24 2020-06-22 Levedura modificada e método para aumentar o teor de lisina em coprodutos de fermentação

Country Status (7)

Country Link
US (1) US20220251582A1 (pt)
EP (1) EP3987017A1 (pt)
CN (1) CN114302958A (pt)
AU (1) AU2020301126A1 (pt)
BR (1) BR112021026266A2 (pt)
CA (1) CA3144620A1 (pt)
WO (1) WO2020263720A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114921506A (zh) * 2022-06-24 2022-08-19 吉林梅花氨基酸有限责任公司 一种含有酒糟水解液的发酵培养基及赖氨酸发酵生产方法
CN116732116A (zh) * 2023-08-11 2023-09-12 齐齐哈尔龙江阜丰生物科技有限公司 一种l-赖氨酸和酵母联合生产的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2060632A1 (en) 2007-10-29 2009-05-20 Technische Universität Berlin Method of modifying a yeast cell for the production of ethanol
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
CN103649321B (zh) 2011-04-05 2019-07-16 拉勒曼德匈牙利流动性管理有限责任公司 用于通过添加交替电子受体改善微生物中的产品收率和产量的方法
FI3033413T4 (fi) 2013-08-15 2023-08-31 Menetelmiä tuotesaannon ja tuotannon parantamiseksi mikro-organismissa glyserolikierrätyksellä
BR112016021857B1 (pt) 2014-03-28 2024-03-12 Danisco Us Inc Célula de levedura recombinante e método para produzir etanol a partir de glicose
WO2016210343A1 (en) * 2015-06-25 2016-12-29 Amyris, Inc. Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

Also Published As

Publication number Publication date
WO2020263720A1 (en) 2020-12-30
AU2020301126A1 (en) 2022-01-27
CA3144620A1 (en) 2020-12-30
CN114302958A (zh) 2022-04-08
US20220251582A1 (en) 2022-08-11
EP3987017A1 (en) 2022-04-27

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