BR112021025474A2 - Mutantes estabilizados de lactonase de quorum quenching e uso seu em tratamento de patógenos - Google Patents

Mutantes estabilizados de lactonase de quorum quenching e uso seu em tratamento de patógenos

Info

Publication number
BR112021025474A2
BR112021025474A2 BR112021025474A BR112021025474A BR112021025474A2 BR 112021025474 A2 BR112021025474 A2 BR 112021025474A2 BR 112021025474 A BR112021025474 A BR 112021025474A BR 112021025474 A BR112021025474 A BR 112021025474A BR 112021025474 A2 BR112021025474 A2 BR 112021025474A2
Authority
BR
Brazil
Prior art keywords
lactonase
lactonases
mutated
treating
quorum quenching
Prior art date
Application number
BR112021025474A
Other languages
English (en)
Inventor
David Gurevich
Livnat Afriat-Jurnou
Maayan Erov
Dafny Yelin Mery
Original Assignee
Migal Galilee Res Institute Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Migal Galilee Res Institute Ltd filed Critical Migal Galilee Res Institute Ltd
Publication of BR112021025474A2 publication Critical patent/BR112021025474A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01081—Quorum-quenching N-acyl-homoserine lactonase (3.1.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/08—Phosphoric triester hydrolases (3.1.8)
    • C12Y301/08001—Aryldialkylphosphatase (3.1.8.1), i.e. paraoxonase
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Abstract

mutantes estabilizados de lactonase de quorum quenching e uso seu em tratamento de patógenos. lactonases semelhantes à fosfotriesterase mutadas ou fragmentos funcionais seus são fornecidas, bem como moléculas de ácido nucleico e vetores que as codificam. ademais, métodos para tratar ou prevenir a infecção de uma bactéria em um hospedeiro, tal como uma planta ou uma parte, órgão ou material de propagação de planta seu compreendendo a aplicação das referidas lactonases semelhantes à fosfotriesterase mutadas ou a enzima de tipo selvagem são fornecidos além de células que expressam as lactonases semelhantes à fosfotriesterase mutadas e métodos para produzi-las.
BR112021025474A 2019-06-17 2020-06-17 Mutantes estabilizados de lactonase de quorum quenching e uso seu em tratamento de patógenos BR112021025474A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962862348P 2019-06-17 2019-06-17
PCT/IL2020/050673 WO2020255131A1 (en) 2019-06-17 2020-06-17 Stabilized mutants of quorum quenching lactonase and use thereof in treatment of pathogens

Publications (1)

Publication Number Publication Date
BR112021025474A2 true BR112021025474A2 (pt) 2022-02-01

Family

ID=74037377

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021025474A BR112021025474A2 (pt) 2019-06-17 2020-06-17 Mutantes estabilizados de lactonase de quorum quenching e uso seu em tratamento de patógenos

Country Status (10)

Country Link
US (1) US20230240305A1 (pt)
EP (1) EP3983535A4 (pt)
JP (1) JP2022536948A (pt)
CN (1) CN114302959A (pt)
AU (1) AU2020295036A1 (pt)
BR (1) BR112021025474A2 (pt)
CA (1) CA3143688A1 (pt)
IL (1) IL288806A (pt)
WO (1) WO2020255131A1 (pt)
ZA (1) ZA202110356B (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022256087A2 (en) * 2021-04-16 2022-12-08 Ginkgo Bioworks, Inc. Organophosphorus nerve agent hydrolyzing enzymes

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPR502301A0 (en) * 2001-05-15 2001-06-07 Commonwealth Scientific And Industrial Research Organisation Phosphotriesterase from Agrobacterium radiobacter P230
FR2915489B1 (fr) * 2007-04-27 2009-07-31 Univ Henri Poincare Nancy I Et Phosphotriesterases hyperthermophiles mutees et leurs utilisations
KR101141787B1 (ko) * 2009-11-04 2012-05-04 한국과학기술원 쿼럼센싱 저해제를 스크리닝하는 방법
AU2012229063A1 (en) * 2011-03-15 2013-09-19 The Regents Of The University Of California Mutant cells for protein secretion and lignocellulose degradation
CN102796714B (zh) * 2012-01-18 2014-06-25 上海交通大学 磷酸三酯酶突变体及其制备方法和应用
CN102965357A (zh) * 2012-12-03 2013-03-13 中国农业科学院饲料研究所 具底物特异性的N-酰基高丝氨酸内酯酶QsdA-RH5及其编码基因与应用
EP2789344A1 (en) * 2013-04-12 2014-10-15 Centre National De La Recherche Scientifique Sulfolobal Phosphotriesterase-Like (PLL) lactonases activity having enhanced properties and the uses thereof
ES2528673B1 (es) * 2013-07-11 2015-11-24 Universidade De Santiago De Compostela Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones
EP2832852A1 (en) * 2013-07-31 2015-02-04 Centre National de la Recherche Scientifique (CNRS) Vulcanisaetal Phosphotriesterase-like Lactonases (PLL) activity having enchanced properties and the uses thereof
US9790254B2 (en) * 2015-05-10 2017-10-17 Ramot At Tel-Aviv University Ltd. Self-assembled peptide nanostructures
US10081800B1 (en) * 2017-08-03 2018-09-25 Fornia Biosolutions, Inc. Lactonase enzymes and methods of using same
CN109182242A (zh) * 2018-09-25 2019-01-11 江南大学 一种异源表达磷酸三酯酶的重组枯草芽孢杆菌

Also Published As

Publication number Publication date
EP3983535A1 (en) 2022-04-20
CN114302959A (zh) 2022-04-08
EP3983535A4 (en) 2023-07-12
JP2022536948A (ja) 2022-08-22
AU2020295036A1 (en) 2022-01-06
CA3143688A1 (en) 2020-12-24
AU2020295036A2 (en) 2022-05-19
ZA202110356B (en) 2023-10-25
US20230240305A1 (en) 2023-08-03
WO2020255131A1 (en) 2020-12-24
IL288806A (en) 2022-02-01

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