BR112021006248A2 - cepa mutante de trichoderma reesei e métodos para produzir uma proteína e para produzir uma celulase - Google Patents

cepa mutante de trichoderma reesei e métodos para produzir uma proteína e para produzir uma celulase

Info

Publication number
BR112021006248A2
BR112021006248A2 BR112021006248A BR112021006248A BR112021006248A2 BR 112021006248 A2 BR112021006248 A2 BR 112021006248A2 BR 112021006248 A BR112021006248 A BR 112021006248A BR 112021006248 A BR112021006248 A BR 112021006248A BR 112021006248 A2 BR112021006248 A2 BR 112021006248A2
Authority
BR
Brazil
Prior art keywords
mutant strain
making
cellulase
protein
methods
Prior art date
Application number
BR112021006248A
Other languages
English (en)
Inventor
Yamada Katsushige
Hiramatsu Shingo
Kagawa Yusuke
Original Assignee
Toray Industries
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries filed Critical Toray Industries
Publication of BR112021006248A2 publication Critical patent/BR112021006248A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

cepa mutante de trichoderma reeseie métodos para produzir uma proteína e para produzir uma celulase. a presente invenção refere-se a uma cepa mutante de trichoderma reesei que tem uma mutação que elimina ou reduz uma função de um polipeptídeo que compreende a sequência de aminoácidos mostrada na seq id no: 8. de preferência, a referida cepa mutante exibe uma variação em um ou mais polipeptídeos escolhidos a partir de polipeptídeos que consistem em uma sequência de aminoácidos representada por qualquer uma das seq id nos, 6, 7, 8, 9 ou 10. proteínas, especialmente celulases, podem ser produzidas usando a referida cepa mutante, enquanto a viscosidade de um líquido de cultivo é mantida baixa durante o cultivo.
BR112021006248A 2018-10-11 2019-10-10 cepa mutante de trichoderma reesei e métodos para produzir uma proteína e para produzir uma celulase BR112021006248A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018192641 2018-10-11
PCT/JP2019/039935 WO2020075788A1 (ja) 2018-10-11 2019-10-10 トリコデルマ・リーセイの変異株およびタンパク質の製造方法

Publications (1)

Publication Number Publication Date
BR112021006248A2 true BR112021006248A2 (pt) 2021-07-06

Family

ID=70164559

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021006248A BR112021006248A2 (pt) 2018-10-11 2019-10-10 cepa mutante de trichoderma reesei e métodos para produzir uma proteína e para produzir uma celulase

Country Status (5)

Country Link
US (1) US20210388410A1 (pt)
JP (1) JPWO2020075788A1 (pt)
CN (1) CN112805366A (pt)
BR (1) BR112021006248A2 (pt)
WO (1) WO2020075788A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7334620B2 (ja) * 2018-03-26 2023-08-29 東レ株式会社 トリコデルマ・リーセイ変異株およびタンパク質の製造方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615511C (en) * 2005-07-19 2014-03-18 Hokko Chemical Industry Co., Ltd. Method for fermentative production of n-acetyl-d-glucosamine by microorganism
CA2833539C (en) * 2011-04-22 2021-04-27 Danisco Us Inc. Filamentous fungi having an altered viscosity phenotype
CN108795988B (zh) * 2017-05-04 2022-07-29 中国科学院分子植物科学卓越创新中心 木聚糖酶专性转录抑制因子及其在里氏木霉改造中的应用
BR112021003456A2 (pt) * 2018-08-29 2021-05-18 Toray Industries, Inc. cepa mutante de trichoderma reesei e métodos para produzir uma proteína, para produzir celulase e para produzir açúcar

Also Published As

Publication number Publication date
CN112805366A (zh) 2021-05-14
WO2020075788A1 (ja) 2020-04-16
JPWO2020075788A1 (ja) 2021-09-02
US20210388410A1 (en) 2021-12-16

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]