BR112018013153A2 - método para a purificação de ácidos nucleicos, kit para purificar ácidos nucleicos e cartucho para executar o método - Google Patents
método para a purificação de ácidos nucleicos, kit para purificar ácidos nucleicos e cartucho para executar o métodoInfo
- Publication number
- BR112018013153A2 BR112018013153A2 BR112018013153-9A BR112018013153A BR112018013153A2 BR 112018013153 A2 BR112018013153 A2 BR 112018013153A2 BR 112018013153 A BR112018013153 A BR 112018013153A BR 112018013153 A2 BR112018013153 A2 BR 112018013153A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- acid purification
- elution buffer
- cartridge
- purification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15202803.1 | 2015-12-28 | ||
| EP15202803 | 2015-12-28 | ||
| PCT/EP2016/082733 WO2017114844A1 (en) | 2015-12-28 | 2016-12-27 | Nucleic acid purification system using a single wash and elution buffer solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112018013153A2 true BR112018013153A2 (pt) | 2018-12-11 |
Family
ID=55229486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112018013153-9A BR112018013153A2 (pt) | 2015-12-28 | 2016-12-27 | método para a purificação de ácidos nucleicos, kit para purificar ácidos nucleicos e cartucho para executar o método |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11427815B2 (ru) |
| EP (1) | EP3397763B1 (ru) |
| JP (1) | JP7068183B2 (ru) |
| CN (1) | CN108473983B (ru) |
| BR (1) | BR112018013153A2 (ru) |
| RU (1) | RU2766005C2 (ru) |
| WO (1) | WO2017114844A1 (ru) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102017206155A1 (de) * | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| CN112239775A (zh) * | 2020-11-12 | 2021-01-19 | 苏州创澜生物科技有限公司 | 一种用于核酸提取的洗涤缓冲液 |
| CN114350652A (zh) * | 2021-12-23 | 2022-04-15 | 力因精准医疗产品（上海）有限公司 | 血液游离dna提取试剂盒及核酸提取方法 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4843155A (en) * | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| US5945515A (en) * | 1995-07-31 | 1999-08-31 | Chomczynski; Piotr | Product and process for isolating DNA, RNA and proteins |
| RU2119954C1 (ru) * | 1996-12-27 | 1998-10-10 | Всероссийский научно-исследовательский институт защиты животных | Способ выделения и очистки нуклеиновых кислот |
| US6120985A (en) | 1997-10-31 | 2000-09-19 | Bbi Bioseq, Inc. | Pressure-enhanced extraction and purification |
| US6111096A (en) * | 1997-10-31 | 2000-08-29 | Bbi Bioseq, Inc. | Nucleic acid isolation and purification |
| EP0967218A1 (en) | 1998-05-26 | 1999-12-29 | Academisch Ziekenhuis Bij De Universiteit Van Amsterdam | Silica-guanidiniumthiocyanate DNA isolation using casein |
| CA3198754A1 (en) | 2004-05-03 | 2005-11-17 | Handylab, Inc. | A microfluidic device and methods for processing polynucleotide-containing samples |
| WO2005121336A1 (ja) | 2004-06-08 | 2005-12-22 | Toagosei Co., Ltd. | 環境dnaの精製方法及び環境dnaからのタンパク質をコードする遺伝子の効率的なスクリーニング方法 |
| EP1882739A1 (en) * | 2006-06-30 | 2008-01-30 | Qiagen GmbH | Nucleic acid extraction method |
| GB0814570D0 (en) * | 2008-08-08 | 2008-09-17 | Diagnostics For The Real World | Isolation of nucleic acid |
| DE102008063003A1 (de) * | 2008-12-23 | 2010-06-24 | Qiagen Gmbh | Nukleinsäureaufreinigungsverfahren |
| NZ594443A (en) * | 2009-02-03 | 2014-06-27 | Netbio Inc | Nucleic acid purification |
| CN102753705A (zh) | 2009-12-14 | 2012-10-24 | B·吴 | 分离核酸物质的方法和材料 |
| AU2012220825B2 (en) | 2011-02-21 | 2015-12-17 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| US20130122496A1 (en) | 2011-09-30 | 2013-05-16 | Blood Cell Storage, Inc. | Storage of nucleic acid |
| EP2771462A4 (en) * | 2011-10-27 | 2015-04-08 | Ge Healthcare Bio Sciences Ab | PURIFICATION OF A NUCLEIC ACID |
| JP5954532B2 (ja) | 2012-04-05 | 2016-07-20 | セイコーエプソン株式会社 | 核酸抽出用デバイス、核酸抽出用キット、核酸抽出用装置及び核酸抽出方法 |
| ES2741749T3 (es) | 2012-10-25 | 2020-02-12 | Neumodx Molecular Inc | Método y materiales para el aislamiento de materiales de ácido nucleico |
| US9455053B2 (en) * | 2013-09-16 | 2016-09-27 | Westinghouse Electric Company Llc | SiC matrix fuel cladding tube with spark plasma sintered end plugs |
| CN103834637A (zh) | 2014-03-20 | 2014-06-04 | 陆欣华 | 一种磁性纳米颗粒对核酸的提取方法和应用 |
| US9510347B2 (en) * | 2014-05-08 | 2016-11-29 | Cisco Technology, Inc. | Timeslot distribution in a distributed routing protocol for deterministic wireless networks |
-
2016
- 2016-12-27 EP EP16822692.6A patent/EP3397763B1/en active Active
- 2016-12-27 RU RU2018127652A patent/RU2766005C2/ru active
- 2016-12-27 CN CN201680076992.3A patent/CN108473983B/zh active Active
- 2016-12-27 US US16/065,843 patent/US11427815B2/en active Active
- 2016-12-27 JP JP2018552915A patent/JP7068183B2/ja active Active
- 2016-12-27 BR BR112018013153-9A patent/BR112018013153A2/pt unknown
- 2016-12-27 WO PCT/EP2016/082733 patent/WO2017114844A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| EP3397763A1 (en) | 2018-11-07 |
| WO2017114844A1 (en) | 2017-07-06 |
| RU2766005C2 (ru) | 2022-02-07 |
| US20190300873A1 (en) | 2019-10-03 |
| JP7068183B2 (ja) | 2022-05-16 |
| CN108473983A (zh) | 2018-08-31 |
| CN108473983B (zh) | 2022-09-13 |
| RU2018127652A3 (ru) | 2020-05-13 |
| RU2018127652A (ru) | 2020-01-30 |
| EP3397763B1 (en) | 2020-10-28 |
| JP2019504640A (ja) | 2019-02-21 |
| US11427815B2 (en) | 2022-08-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B350 | Update of information on the portal [chapter 15.35 patent gazette] |