BR112018001373A2 - molécula de ácido nucleico isolada, cassete de expressão, vetor de expressão, célula hospedeira, métodos para produzir uma planta transgênica e uma semente transgênica, para controlar a população de uma praga lepidóptera, para exterminar uma praga lepidóptera e para reduzir os danos de uma peste lepidóptera a uma planta, planta transgênica, sonda, e, par de iniciadores. - Google Patents

molécula de ácido nucleico isolada, cassete de expressão, vetor de expressão, célula hospedeira, métodos para produzir uma planta transgênica e uma semente transgênica, para controlar a população de uma praga lepidóptera, para exterminar uma praga lepidóptera e para reduzir os danos de uma peste lepidóptera a uma planta, planta transgênica, sonda, e, par de iniciadores.

Info

Publication number
BR112018001373A2
BR112018001373A2 BR112018001373-0A BR112018001373A BR112018001373A2 BR 112018001373 A2 BR112018001373 A2 BR 112018001373A2 BR 112018001373 A BR112018001373 A BR 112018001373A BR 112018001373 A2 BR112018001373 A2 BR 112018001373A2
Authority
BR
Brazil
Prior art keywords
gene
cry1ab
application
gene encoding
cry1aczm
Prior art date
Application number
BR112018001373-0A
Other languages
English (en)
Chinese (zh)
Other versions
BR112018001373B1 (pt
Inventor
Liu Bolin
Tan Chao
Yang Qianqian
Xu Jieting
Wen Qin
Qiu Long
Ma Chonglie
Zhang Wanggen
Original Assignee
China National Seed Group Corporation, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China National Seed Group Corporation, Ltd. filed Critical China National Seed Group Corporation, Ltd.
Publication of BR112018001373A2 publication Critical patent/BR112018001373A2/pt
Publication of BR112018001373B1 publication Critical patent/BR112018001373B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pest Control & Pesticides (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Mycology (AREA)

Abstract

é provido um gene cry1ab/cry1aczm, um kit de expressão, vetor de expressão e célula hospedeira compreendendo o gene, e um uso do gene no melhoramento vegetal. proteínas codificadas pelo gene cry1ab/cry1aczm podem ser expressadas in monocotiledôneas, e adicionalmente usadas para melhorar monocotiledôneas resistentes a insetos de gene trans-cry1ab/cry1aczm. experimentos de bioensaio provam que um produto de expressão do gene cry1ab/cry1aczm tem um efeito de exterminação em pragas lepidópteras.
BR112018001373-0A 2015-07-23 2016-07-22 Molécula de ácido nucleico isolada, cassete de expressão, vetor de expressão, célula hospedeira, métodos para produzir uma planta transgênica e uma semente transgênica, para controlar a população de uma praga lepidóptera, para exterminar uma praga lepidóptera e para reduzir os danos de uma peste lepidóptera a uma planta, e, par de iniciadores BR112018001373B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510437025.2 2015-07-23
CN201510437025.2A CN106701789B (zh) 2015-07-23 2015-07-23 编码苏云金芽孢杆菌晶体蛋白质的基因及其的应用
PCT/CN2016/090978 WO2017012577A1 (zh) 2015-07-23 2016-07-22 编码苏云金芽孢杆菌晶体蛋白质的基因及其的应用

Publications (2)

Publication Number Publication Date
BR112018001373A2 true BR112018001373A2 (pt) 2018-09-11
BR112018001373B1 BR112018001373B1 (pt) 2023-12-26

Family

ID=57833734

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018001373-0A BR112018001373B1 (pt) 2015-07-23 2016-07-22 Molécula de ácido nucleico isolada, cassete de expressão, vetor de expressão, célula hospedeira, métodos para produzir uma planta transgênica e uma semente transgênica, para controlar a população de uma praga lepidóptera, para exterminar uma praga lepidóptera e para reduzir os danos de uma peste lepidóptera a uma planta, e, par de iniciadores

Country Status (4)

Country Link
US (1) US10626411B2 (pt)
CN (1) CN106701789B (pt)
BR (1) BR112018001373B1 (pt)
WO (1) WO2017012577A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110144361A (zh) * 2018-02-11 2019-08-20 中国种子集团有限公司 Cry1Ab/Cry1AcZM基因的抗粘虫新用途
CN109776659B (zh) * 2019-03-14 2021-01-29 中国农业科学院生物技术研究所 cry2Ah-vp基因在抗黏虫中的应用
CN112375838A (zh) * 2020-11-19 2021-02-19 中国热带农业科学院热带生物技术研究所 一种转基因甘蔗scag2的t-dna侧翼序列及其转化事件特异性鉴定方法
CN112391490A (zh) * 2020-11-19 2021-02-23 中国热带农业科学院热带生物技术研究所 一种转基因甘蔗事件scag5的外源插入片段侧翼序列及其品系特异性检测方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1037913C (zh) * 1995-12-28 1998-04-01 中国农业科学院生物技术研究中心 编码杀虫蛋白质的融合基因和表达载体及其应用
US6218188B1 (en) * 1997-11-12 2001-04-17 Mycogen Corporation Plant-optimized genes encoding pesticidal toxins
CN1230534C (zh) * 2002-08-28 2005-12-07 中国农业科学院生物技术研究所 具有两种杀虫机理的融合杀虫蛋白质基因及其应用
CN102031266B (zh) 2010-03-25 2013-05-01 浙江大学 抗虫融合基因、融合蛋白质及其应用

Also Published As

Publication number Publication date
CN106701789A (zh) 2017-05-24
US10626411B2 (en) 2020-04-21
BR112018001373B1 (pt) 2023-12-26
WO2017012577A1 (zh) 2017-01-26
US20180362998A1 (en) 2018-12-20
CN106701789B (zh) 2019-10-08

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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