BR112017023011B1 - Dispositivo de cultura para enumerar colônias de microrganismos microaerofílicos e método de detecção de um microrganismo microaerofílico em uma amostra - Google Patents
Dispositivo de cultura para enumerar colônias de microrganismos microaerofílicos e método de detecção de um microrganismo microaerofílico em uma amostra Download PDFInfo
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562154299P | 2015-04-29 | 2015-04-29 | |
| US62/154,299 | 2015-04-29 | ||
| PCT/US2016/029306 WO2016176176A1 (en) | 2015-04-29 | 2016-04-26 | Culture device for anaerobic microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112017023011A2 BR112017023011A2 (pt) | 2018-07-03 |
| BR112017023011B1 true BR112017023011B1 (pt) | 2022-04-05 |
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Family Applications (1)
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| BR112017023011-9A BR112017023011B1 (pt) | 2015-04-29 | 2016-04-26 | Dispositivo de cultura para enumerar colônias de microrganismos microaerofílicos e método de detecção de um microrganismo microaerofílico em uma amostra |
Country Status (7)
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| US (1) | US20180094291A1 (https=) |
| EP (1) | EP3289062A1 (https=) |
| JP (1) | JP6920212B2 (https=) |
| KR (1) | KR102645495B1 (https=) |
| CN (1) | CN107532121A (https=) |
| BR (1) | BR112017023011B1 (https=) |
| WO (1) | WO2016176176A1 (https=) |
Families Citing this family (8)
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| EP3286731B1 (en) | 2015-04-23 | 2020-09-16 | BD Kiestra B.V. | Colony contrast gathering |
| US10723992B2 (en) | 2015-04-29 | 2020-07-28 | 3M Innovative Properties Company | Thin film culture device with carbon dioxide generant |
| WO2017058737A1 (en) | 2015-09-28 | 2017-04-06 | 3M Innovative Properties Company | Self-contained anaerobic culture device with microcompartments |
| WO2018096829A1 (en) * | 2016-11-28 | 2018-05-31 | Jnc Corporation | Culture equipment of microorganisms and method for counting number of microorganisms using same |
| CN107418887A (zh) * | 2017-09-15 | 2017-12-01 | 厦门安捷致善医学数据科技有限公司 | 一种粪便微生物提取过程中配置厌氧装置 |
| US12391907B2 (en) | 2017-12-13 | 2025-08-19 | Neogen Food Safety Us Holdco Corporation | Water-reconstitutable culture medium resistant to liquefaction by microorganisms |
| CN109371098A (zh) * | 2018-11-14 | 2019-02-22 | 浙江海洋大学 | 一种石油腐蚀性微生物检测工具箱用微生物活性显色增强方法 |
| CN109576130A (zh) * | 2018-12-18 | 2019-04-05 | 东北农业大学 | 人为制造微氧环境纯化微氧细菌的装置 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3338794A (en) | 1964-11-23 | 1967-08-29 | Swift & Co | Culturing anaerobic bacteria |
| JPS5739776A (en) * | 1980-08-20 | 1982-03-05 | Mitsubishi Gas Chem Co Inc | Cultivation of anaerobic bacteria |
| US4565783A (en) | 1981-01-27 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Dry culture media |
| JPS5856679A (ja) * | 1981-09-30 | 1983-04-04 | Meito Kk | 嫌気性菌培養方法及び雰囲気調整剤 |
| US5034331A (en) * | 1981-12-04 | 1991-07-23 | Fairleigh Dickinson Laboratories, Inc. | Compositions and methods for culturing microorganisms requiring special gaseous environments |
| US5089413A (en) | 1989-05-19 | 1992-02-18 | Minnesota Mining And Manufacturing Company | Method and apparatus for culturing with microbiological dry culture medium |
| US5232838A (en) | 1991-12-09 | 1993-08-03 | Minnesota Mining And Manufacturing Company | Culture media device and method of use |
| JPH0775545A (ja) * | 1993-09-09 | 1995-03-20 | Mitsubishi Gas Chem Co Inc | 嫌気性細菌簡易培養袋及びこれを用いた嫌気性細菌培養法 |
| US5830746A (en) * | 1994-05-04 | 1998-11-03 | Oxyrase, Inc. | Apparatus and method for growing anaerobic microorganisms |
| US5601998A (en) | 1994-08-18 | 1997-02-11 | Minnesota Mining & Mfg | Culture medium and device for detection and enumeration of enterobacteriaceae |
| US5681712A (en) * | 1995-06-02 | 1997-10-28 | Minnesota Mining And Manufacturing Company | Surface colony counting device and method of use |
| JPH0965894A (ja) * | 1995-08-31 | 1997-03-11 | Takeda Chem Ind Ltd | 真菌の薬剤感受性測定法 |
| US6037022A (en) * | 1997-09-16 | 2000-03-14 | International Paper Company | Oxygen-scavenging filled polymer blend for food packaging applications |
| AU1157100A (en) * | 1998-10-29 | 2000-05-22 | Connex Gmbh | Detection of acid-resistant micro-organisms in a stool |
| US7374905B2 (en) * | 2000-11-08 | 2008-05-20 | Oxyrase, Inc. | Medium composition, method and device for selectively enhancing the isolation of anaerobic microorganisms contained in a mixed sample with facultative microorganisms |
| US6689438B2 (en) | 2001-06-06 | 2004-02-10 | Cryovac, Inc. | Oxygen detection system for a solid article |
| US20040101954A1 (en) | 2002-11-27 | 2004-05-27 | Graessle Josef A. | Back side plate illumination for biological growth plate scanner |
| US20040102903A1 (en) | 2002-11-27 | 2004-05-27 | Graessle Josef A. | Biological growth plate scanner |
| US7496225B2 (en) | 2003-09-04 | 2009-02-24 | 3M Innovative Properties Company | Biological growth plate scanner with automated intake |
| US20050239200A1 (en) * | 2004-04-23 | 2005-10-27 | Beckwith Scott W | Devices for culturing anaerobic microorganisms and methods of using the same |
| JP2007075018A (ja) * | 2005-09-15 | 2007-03-29 | Eiken Chem Co Ltd | Ｃａｍｐｙｌｏｂａｃｔｅｒｃｏｌｉの検出法 |
| JP2007075017A (ja) * | 2005-09-15 | 2007-03-29 | Eiken Chem Co Ltd | Ｃａｍｐｙｌｏｂａｃｔｅｒｊｅｊｕｎｉの検出法 |
| EP2235203B1 (en) * | 2007-12-21 | 2014-10-08 | 3M Innovative Properties Company | Microbiological systems and methods of fluid sample analysis |
| KR20120039629A (ko) * | 2009-06-15 | 2012-04-25 | 쓰리엠 이노베이티브 프로퍼티즈 컴파니 | 산-생성 박테리아의 검출 |
| JP5519960B2 (ja) * | 2009-06-18 | 2014-06-11 | 日水製薬株式会社 | 乳酸菌検出用培地及び検出法 |
| EP2512674B1 (en) * | 2009-12-15 | 2018-04-04 | Sicpa Holding Sa | Process for assembling elements containing biological substances |
| MX2013013255A (es) * | 2011-05-20 | 2014-01-08 | 3M Innovative Properties Co | Articulos de deteccion de salmonella y metodos de uso. |
| WO2014099644A1 (en) * | 2012-12-20 | 2014-06-26 | 3M Innovative Properties Company | Method of detecting gas-producing microbial colonies |
| EP3060676B1 (en) | 2013-10-24 | 2019-11-20 | 3M Innovative Properties Company | Self-contained anaerobic environment-generating culture devices and methods of use |
| US10723992B2 (en) * | 2015-04-29 | 2020-07-28 | 3M Innovative Properties Company | Thin film culture device with carbon dioxide generant |
-
2016
- 2016-04-26 BR BR112017023011-9A patent/BR112017023011B1/pt active IP Right Grant
- 2016-04-26 CN CN201680024743.XA patent/CN107532121A/zh active Pending
- 2016-04-26 US US15/569,552 patent/US20180094291A1/en not_active Abandoned
- 2016-04-26 WO PCT/US2016/029306 patent/WO2016176176A1/en not_active Ceased
- 2016-04-26 EP EP16725259.2A patent/EP3289062A1/en active Pending
- 2016-04-26 JP JP2017556553A patent/JP6920212B2/ja active Active
- 2016-04-26 KR KR1020177032942A patent/KR102645495B1/ko active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP6920212B2 (ja) | 2021-08-18 |
| JP2018518158A (ja) | 2018-07-12 |
| EP3289062A1 (en) | 2018-03-07 |
| BR112017023011A2 (pt) | 2018-07-03 |
| CN107532121A (zh) | 2018-01-02 |
| US20180094291A1 (en) | 2018-04-05 |
| KR20170138480A (ko) | 2017-12-15 |
| WO2016176176A1 (en) | 2016-11-03 |
| KR102645495B1 (ko) | 2024-03-07 |
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