BR112017020916A2 - materiais e métodos para fermentação de fluxo contínuo estentida de bactérias de genoma reduzido - Google Patents

materiais e métodos para fermentação de fluxo contínuo estentida de bactérias de genoma reduzido

Info

Publication number
BR112017020916A2
BR112017020916A2 BR112017020916A BR112017020916A BR112017020916A2 BR 112017020916 A2 BR112017020916 A2 BR 112017020916A2 BR 112017020916 A BR112017020916 A BR 112017020916A BR 112017020916 A BR112017020916 A BR 112017020916A BR 112017020916 A2 BR112017020916 A2 BR 112017020916A2
Authority
BR
Brazil
Prior art keywords
fermentation
methods
reduced
long
vessel
Prior art date
Application number
BR112017020916A
Other languages
English (en)
Other versions
BR112017020916B1 (pt
Inventor
R Blattner Frederick
Original Assignee
Scarab Genomics Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scarab Genomics Llc filed Critical Scarab Genomics Llc
Publication of BR112017020916A2 publication Critical patent/BR112017020916A2/pt
Publication of BR112017020916B1 publication Critical patent/BR112017020916B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

trata-se de um sistema de fluxo contínuo de dois vasos em conjunto com cepas bacterianas de genoma reduzido de mutação baixa que fornece uma plataforma para fermentações estendidas a longo prazo. tais sistemas exigem modificação de dispositivos de fermentação padrão, como sondas, bombas e sistemas de monitoramento, bem como procedimentos aprimorados para entrega de alimentação, monitoramento de cultura e métodos de coleta de produto. um sistema de dois vasos otimizado para produzir quantidades grandes de produtos de fermentação de volume pequeno, fermentações contínuas de longa duração representam um aprimoramento significativo em relação às estratégias de fermentação existentes. métodos e composições para fermentação de fluxo contínuo a longo prazo com o uso de um aparelho de fermentação de cultura contínua de dois vasos são descritos.
BR112017020916-0A 2015-04-02 2016-04-01 Processo de fermentação contínua para a produção de produto biológico BR112017020916B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562142282P 2015-04-02 2015-04-02
US62/142,282 2015-04-02
PCT/US2016/025588 WO2016161305A1 (en) 2015-04-02 2016-04-01 Materials and methods for extended continuous flow fermentation of reduced genome bacteria

Publications (2)

Publication Number Publication Date
BR112017020916A2 true BR112017020916A2 (pt) 2018-07-10
BR112017020916B1 BR112017020916B1 (pt) 2022-01-25

Family

ID=57007614

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017020916-0A BR112017020916B1 (pt) 2015-04-02 2016-04-01 Processo de fermentação contínua para a produção de produto biológico

Country Status (9)

Country Link
US (2) US10604736B2 (pt)
EP (1) EP3277791B1 (pt)
JP (2) JP6744872B2 (pt)
KR (1) KR102351231B1 (pt)
CN (1) CN107532123A (pt)
AU (1) AU2016242996B2 (pt)
BR (1) BR112017020916B1 (pt)
CA (1) CA2981583C (pt)
WO (1) WO2016161305A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019050872A1 (en) 2017-09-05 2019-03-14 Scarab Genomics, Llc METHODS AND COMPOSITIONS FOR ENHANCED EXPRESSION OF RECOMBINANT PROTEINS

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5470492A (en) * 1977-11-16 1979-06-06 Japan Tobacco Inc Method and appratus for heat sterilization of medium for vegetable tissue culture
GB8531069D0 (en) 1985-12-17 1986-01-29 Celltech Ltd Fermentation processes
CN1215083A (zh) * 1998-07-26 1999-04-28 陶德录 应用恒化器连续培养液体微生物的方法
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US20060199260A1 (en) 2002-05-01 2006-09-07 Zhiyu Zhang Microbioreactor for continuous cell culture
WO2005087940A1 (en) 2004-03-11 2005-09-22 Wisconsin Alumni Research Foundation Genetically altered microorganisms with modified metabolism
EP1885847B1 (en) * 2005-05-26 2011-07-06 Cytos Biotechnology AG Scalable fermentation process
CA2626900C (en) 2005-08-20 2013-07-23 Scarab Genomics, Llc Reduced genome e. coli
US20080318283A1 (en) 2006-02-01 2008-12-25 Clague P. Hodgson Fermentation Process for Continuous Plasmid Dna Production
JP4953769B2 (ja) * 2006-11-02 2012-06-13 三洋電機株式会社 細胞培養装置及び細胞培養カセット
CN201707311U (zh) * 2010-05-11 2011-01-12 中国农业大学 堆肥监测装置
LT2655600T (lt) * 2010-12-21 2017-05-25 Glycotope Gmbh Žmogaus mieloidinės leukemijos ląstelių ir iš jų gautų ląstelių kultivavimo būdai
KR102043891B1 (ko) 2011-10-20 2019-11-12 스캐랩 게노믹스, 엘엘씨 개선된 유전적 안전성을 가지는 감소된 게놈 박테리아
ES2842350T3 (es) 2013-03-19 2021-07-13 Cmc Biologics As Un procedimiento para la producción de un producto (por ejemplo, polipéptido) en un proceso de fermentación de cultivo celular continuo
US9902965B2 (en) 2013-11-14 2018-02-27 Scarab Genomics, Llc Bacteria with improved metabolic capacity
AU2015225439B2 (en) * 2014-03-03 2020-12-17 Scarab Genomics, Llc Enhanced production of recombinant CRM197 in E. coli
CN203807468U (zh) * 2014-04-29 2014-09-03 青岛格瑞盛环保工程有限公司 具有两种进料结构的沼气发酵罐

Also Published As

Publication number Publication date
JP2020171331A (ja) 2020-10-22
AU2016242996A1 (en) 2017-11-02
WO2016161305A1 (en) 2016-10-06
US20200216798A1 (en) 2020-07-09
US10604736B2 (en) 2020-03-31
JP2018509926A (ja) 2018-04-12
KR20170133397A (ko) 2017-12-05
EP3277791A4 (en) 2018-10-31
CN107532123A (zh) 2018-01-02
BR112017020916B1 (pt) 2022-01-25
CA2981583C (en) 2022-11-15
CA2981583A1 (en) 2016-10-06
JP6744872B2 (ja) 2020-08-19
EP3277791B1 (en) 2022-12-14
EP3277791A1 (en) 2018-02-07
AU2016242996B2 (en) 2021-02-18
KR102351231B1 (ko) 2022-01-14
US20180087018A1 (en) 2018-03-29
US11142745B2 (en) 2021-10-12

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/04/2016, OBSERVADAS AS CONDICOES LEGAIS.