JPS5470492A - Method and appratus for heat sterilization of medium for vegetable tissue culture - Google Patents

Method and appratus for heat sterilization of medium for vegetable tissue culture

Info

Publication number
JPS5470492A
JPS5470492A JP13674377A JP13674377A JPS5470492A JP S5470492 A JPS5470492 A JP S5470492A JP 13674377 A JP13674377 A JP 13674377A JP 13674377 A JP13674377 A JP 13674377A JP S5470492 A JPS5470492 A JP S5470492A
Authority
JP
Japan
Prior art keywords
phosphate
medium
tissue culture
sterilizer
appratus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP13674377A
Other languages
Japanese (ja)
Other versions
JPS5547874B2 (en
Inventor
Shizuo Sugita
Shoji Azechi
Yoshinari Yamazaki
Yuichi Yoshida
Toshio Hashimoto
Tsugio Yuyama
Satoshi Nagatsuka
Kiyoshi Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Original Assignee
Japan Tobacco Inc
Japan Tobacco and Salt Public Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc, Japan Tobacco and Salt Public Corp filed Critical Japan Tobacco Inc
Priority to JP13674377A priority Critical patent/JPS5470492A/en
Publication of JPS5470492A publication Critical patent/JPS5470492A/en
Publication of JPS5547874B2 publication Critical patent/JPS5547874B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: To prevent the deposition of phosphate scale to the heating surface of a sterilizer, by separately sterilizing the phosphate component of the culturing medium and the other components, and supplying the sterilized components to a culturing tank.
CONSTITUTION: Storage tanks 1, 2, and 3 contain a medium for vegetable tissue culture which lacks phosphate component, clean water, and a solution of phosphate, respectively. The contents of the tanks 1, 2, and 3 are sent to a continuous heat sterilizer 8 continuously and separately, by a metering pump 7 with a sequence of 1,2,3,2,1,2,3, etc., by automatically opening the valves 4,5 6. The undesirable residue of the phosphate on the heating surface of the sterilizer is washed off each time, and the deposition of scale prevented.
COPYRIGHT: (C)1979,JPO&Japio
JP13674377A 1977-11-16 1977-11-16 Method and appratus for heat sterilization of medium for vegetable tissue culture Granted JPS5470492A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13674377A JPS5470492A (en) 1977-11-16 1977-11-16 Method and appratus for heat sterilization of medium for vegetable tissue culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13674377A JPS5470492A (en) 1977-11-16 1977-11-16 Method and appratus for heat sterilization of medium for vegetable tissue culture

Publications (2)

Publication Number Publication Date
JPS5470492A true JPS5470492A (en) 1979-06-06
JPS5547874B2 JPS5547874B2 (en) 1980-12-02

Family

ID=15182457

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13674377A Granted JPS5470492A (en) 1977-11-16 1977-11-16 Method and appratus for heat sterilization of medium for vegetable tissue culture

Country Status (1)

Country Link
JP (1) JPS5470492A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170133397A (en) * 2015-04-02 2017-12-05 스캐랩 게노믹스, 엘엘씨 Materials and methods for extended continuous flow culture of reduced genomic bacteria
US10527902B2 (en) 2006-09-29 2020-01-07 Semiconductor Energy Laboratory Co., Ltd. Display device and electronic device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4912084A (en) * 1972-03-22 1974-02-02
JPS5228986A (en) * 1975-08-30 1977-03-04 Japan Tobacco Inc Device for continuous culture of microorganisms or animal and plant ti ssues

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4912084A (en) * 1972-03-22 1974-02-02
JPS5228986A (en) * 1975-08-30 1977-03-04 Japan Tobacco Inc Device for continuous culture of microorganisms or animal and plant ti ssues

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10527902B2 (en) 2006-09-29 2020-01-07 Semiconductor Energy Laboratory Co., Ltd. Display device and electronic device
KR20170133397A (en) * 2015-04-02 2017-12-05 스캐랩 게노믹스, 엘엘씨 Materials and methods for extended continuous flow culture of reduced genomic bacteria
CN107532123A (en) * 2015-04-02 2018-01-02 斯卡拉布基因组有限责任公司 Material and method for the extension continuous stream fermentation of the bacterium of genome reduction
JP2018509926A (en) * 2015-04-02 2018-04-12 スカラブ ゲノミクス, エルエルシー Materials and methods for extended continuous flow fermentation of reduced genome bacteria
US11142745B2 (en) 2015-04-02 2021-10-12 Scarab Genomics, Llc Materials and methods for extended continuous flow fermentation of reduced genome bacteria

Also Published As

Publication number Publication date
JPS5547874B2 (en) 1980-12-02

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