KR960040140A

KR960040140A - Spray Plant Tissue Culture Device

Info

Publication number: KR960040140A
Application number: KR1019950013716A
Authority: KR
Inventors: 최상진
Original assignee: 최상진
Priority date: 1995-05-22
Filing date: 1995-05-22
Publication date: 1996-12-17
Also published as: KR100189574B1

Abstract

본원의 발명은 식물조직배양 중 특히 번식체의 대량생산에 가장 효과적으로 사용될 수 있는 장치이다. 조직배양의 대량생산은 필연적으로 액체배양일 수 밖에 없으며, 이를 위해서 종래에는 배양체를 배지와 함께 용기에 넣고 진탕하거나 공기를 배지 속에 주입시키는 방식이었다.The present invention is a device that can be most effectively used in the mass production of propagules, especially in plant tissue culture. Mass production of tissue culture is inevitably liquid culture, and for this purpose, conventionally, a culture was put in a container with a medium and shaken or air was injected into the medium.

여기서 배양체는 배지와 완전히 접촉하게 되므로 양분의 흡수기회는 충분하지만, 공기와 접촉할 기회는 아주 적다. 식물조직을 애체배지 속에 넣고 기포를 아무리 많이 발생시켜 주어도 조직이 자연상태에서와 같은 충분한 산소를 이용할 수 없을 것은 확실하다. 이런 상황에서는 양분의 흡수도 잘 이루어질 수 없다.Here, the culture is in full contact with the medium, so the absorption opportunity of the nutrient is sufficient, but the chance of contact with the air is very small. It is clear that no matter how much air bubbles are put in plant tissues, tissues will not be able to use enough oxygen as in nature. In this situation, the absorption of nutrients is also poor.

만약 번식체를 공기 중에 안정된 상태로 두고, 배지와 공기에 충분히 노출시키면 잘 자랄 것이다. 본 발명은 바로 이런 배양환경을 만들어 주는 장치이다. 즉, 조직(배양체)을 배양실(용기 또는 탱크)내의 그물시렁에 놓고, 배지는 순환모타로 분무하며, 공기는 공기펌프로 배양실의 대기를 새롭게 한다.If the propagules are kept stable in air and exposed to medium and air enough, they will grow well. The present invention is a device for making such a culture environment. That is, the tissue (culture medium) is placed in a net cache in the culture chamber (container or tank), the medium is sprayed with a circulating motor, and the air refreshes the atmosphere of the culture chamber with an air pump.

이 장치를 이용함으로서 지금까지 여러 가지 유용식물을 조직배양할 때 접했던 난해한 문제들을 해결할 수 있다. 그것은 배양체의 생장이 촉진되고, 배양 단위의 확대와 배지교체의 간편등으로 노동력이 크게 감소되면서 조직배양묘의 생산과 이용이 확대되는 효과를 얻을 수 있다.By using this device, it is possible to solve the difficult problems encountered so far in the tissue culture of various useful plants. It is possible to increase the production and utilization of tissue culture seedlings, as the growth of the culture is promoted, and the labor force is greatly reduced due to the expansion of the culture unit and the simplicity of medium replacement.

Description

Spray Plant Tissue Culture Device

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명에 따른 분무식 식물조직배양장치의 도식, 제2도는 배양실의 측면도, 제4도는 배양실 뚜껑 안쪽의 평면도.Figure 1 is a schematic diagram of the spray plant tissue culture apparatus according to the present invention, Figure 2 is a side view of the culture chamber, Figure 4 is a plan view inside the lid of the culture chamber.

Claims

When the plant tissue is cultured in a container or a tank, the culture solution (medium) is sprayed and supplied to the culture while the culture (tissue) is exposed to the air in the culture chamber (3), and the air is supplied separately to the culture chamber.

The culture apparatus of any type according to claim 1, using this principle.

The device according to claim 1 or 2, wherein the culture is placed on a reticulated shelf (8) above the culture (9).

The device according to claim 1 or 2, wherein the supply of the culture liquid is injected into the culture through the spray nozzle (2).

The device according to claim 1 or 2, wherein the medium is circulated through a motor pump (6) outside the culture chamber.

The form according to claim 2, wherein the temperature rise and cooling of the culture chamber are performed at the culture chamber pedestal (22).

4. The device according to claim 3, wherein the injecting of the culture medium into the shelf is via the culture inlet port (21) in the culture chamber wall (20).

※ Note: The disclosure is based on the initial application.