BR112017015180A2 - métodos para controlar a produção de proteases - Google Patents

métodos para controlar a produção de proteases

Info

Publication number
BR112017015180A2
BR112017015180A2 BR112017015180-4A BR112017015180A BR112017015180A2 BR 112017015180 A2 BR112017015180 A2 BR 112017015180A2 BR 112017015180 A BR112017015180 A BR 112017015180A BR 112017015180 A2 BR112017015180 A2 BR 112017015180A2
Authority
BR
Brazil
Prior art keywords
methods
protease production
production
host cells
controlling
Prior art date
Application number
BR112017015180-4A
Other languages
English (en)
Inventor
Paloheimo Marja
Mäkinen Susanna
Punt Peter
Juntunen Kari
Puranen Terhi
Vehmaanperä Jari
Original Assignee
Roal Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roal Oy filed Critical Roal Oy
Publication of BR112017015180A2 publication Critical patent/BR112017015180A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/14—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from fungi, algea or lichens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Abstract

a presente descrição refere-se ao campo de produção de proteínas. ela introduz novas células hospedeiras com baixa atividade de protease, um novo regulador de protease, seu uso em sistemas de expressão e produção de proteínas, e um método para produzir células hospedeiras para produção de proteínas.
BR112017015180-4A 2015-02-20 2016-02-19 métodos para controlar a produção de proteases BR112017015180A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI20155112 2015-02-20
FI20155112 2015-02-20
PCT/FI2016/050108 WO2016132021A1 (en) 2015-02-20 2016-02-19 Methods for controlling protease production

Publications (1)

Publication Number Publication Date
BR112017015180A2 true BR112017015180A2 (pt) 2018-01-16

Family

ID=55485006

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017015180-4A BR112017015180A2 (pt) 2015-02-20 2016-02-19 métodos para controlar a produção de proteases

Country Status (9)

Country Link
US (2) US10883131B2 (pt)
EP (1) EP3259364B1 (pt)
CN (1) CN108138213A (pt)
AU (1) AU2016221605B2 (pt)
BR (1) BR112017015180A2 (pt)
CA (1) CA2976021A1 (pt)
DK (1) DK3259364T3 (pt)
ES (1) ES2815628T3 (pt)
WO (1) WO2016132021A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016132021A1 (en) * 2015-02-20 2016-08-25 Roal Oy Methods for controlling protease production
CN110054684B (zh) * 2019-04-20 2021-07-20 中国农业科学院植物保护研究所 东亚飞蝗丝氨酸蛋白酶抑制剂serpin-5及其编码基因和应用
CA3190516A1 (en) 2020-07-31 2022-02-03 Biotalys NV Expression host
CN114686389A (zh) * 2020-12-25 2022-07-01 江苏东汇生物科技有限公司 一种增强vgbS基因转录水平的谷氨酰胺转氨酶高产菌株及其制备与发酵方法
CN113913413B (zh) * 2021-08-11 2023-10-27 上海雅心生物技术有限公司 一种耐盐的rpk突变体及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5296731A (en) * 1976-02-05 1977-08-13 Green Cross Corp:The Prophylactics and remedies of cyanomycosis
US5000939A (en) * 1984-06-12 1991-03-19 Colgate-Palmolive Company Dentifrice containing stabilized enzyme
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
DE60025467T2 (de) * 1999-02-22 2006-09-21 Novozymes A/S Oxalacetat-hydrolase defiziente fungale wirtszellen
CN102174535B (zh) 2000-03-14 2014-04-09 诺维信公司 在制备多肽的方法中有效的真菌转录激活因子
FI118339B (fi) 2004-09-21 2007-10-15 Ab Enzymes Oy Uusi lakkaasientsyymi ja sen käyttö
WO2006040312A2 (en) * 2004-10-12 2006-04-20 Dsm Ip Assets B.V. Fungal transcriptional activators useful in methods for producing a polypeptide
PT1874927E (pt) 2005-04-29 2014-04-23 Ab Enzymes Oy Celulases melhoradas
CA2994320C (en) * 2005-12-22 2023-05-16 Roal Oy Treatment of cellulosic material and enzymes useful therein
BR112014016587A2 (pt) 2012-01-05 2020-10-27 Novartis Ag células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas
CN102787108B (zh) * 2012-07-27 2013-12-04 中国科学院微生物研究所 来源于里氏木霉的一个蛋白质及其基因的用途
JP6660181B2 (ja) * 2012-12-28 2020-03-11 アーベー・エンザイムス・ゲーエムベーハー トリコデルマリーセイＱＭ６ａおよびその誘導体中の交配障害と関連する遺伝子／遺伝要素ならびにその同定方法
WO2015187697A2 (en) * 2014-06-02 2015-12-10 The Board Of Regents For Oklahoma State University Systems and methods for production and use of fungal glycosyl hydrolases
WO2016132021A1 (en) * 2015-02-20 2016-08-25 Roal Oy Methods for controlling protease production

Also Published As

Publication number Publication date
CA2976021A1 (en) 2016-08-25
AU2016221605B2 (en) 2019-12-05
US10883131B2 (en) 2021-01-05
US20210102231A1 (en) 2021-04-08
EP3259364B1 (en) 2020-07-01
US20180037920A1 (en) 2018-02-08
DK3259364T3 (da) 2020-09-07
EP3259364A1 (en) 2017-12-27
AU2016221605A1 (en) 2017-09-07
ES2815628T3 (es) 2021-03-30
CN108138213A (zh) 2018-06-08
WO2016132021A1 (en) 2016-08-25

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