BR112015029559A2 - partícula similar a vírus, ácido nucleico e métodos para produzir rna de fita dupla e sirna, shrna, sshrna, 1shrna e mirna - Google Patents

partícula similar a vírus, ácido nucleico e métodos para produzir rna de fita dupla e sirna, shrna, sshrna, 1shrna e mirna

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Publication number
BR112015029559A2
BR112015029559A2 BR112015029559A BR112015029559A BR112015029559A2 BR 112015029559 A2 BR112015029559 A2 BR 112015029559A2 BR 112015029559 A BR112015029559 A BR 112015029559A BR 112015029559 A BR112015029559 A BR 112015029559A BR 112015029559 A2 BR112015029559 A2 BR 112015029559A2
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Brazil
Prior art keywords
virus
1shrna
sshrna
shrna
mirna
Prior art date
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BR112015029559A
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English (en)
Inventor
Oates Edward
P Arhancet Juan
Pedro Humberto Arhancet Juan
B Hall Kathleen
delaney Kimberly
Summers Neena
Original Assignee
Apse Llc
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Publication date
Application filed by Apse Llc filed Critical Apse Llc
Publication of BR112015029559A2 publication Critical patent/BR112015029559A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2330/00—Production
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    • C12N2795/00—Bacteriophages
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    • C12N2795/00011—Details
    • C12N2795/00041—Use of virus, viral particle or viral elements as a vector
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    • C12N2795/18122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/18011—Details ssRNA Bacteriophages positive-sense
    • C12N2795/18111—Leviviridae
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    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/18011—Details ssRNA Bacteriophages positive-sense
    • C12N2795/18111—Leviviridae
    • C12N2795/18141—Use of virus, viral particle or viral elements as a vector
    • C12N2795/18142—Use of virus, viral particle or viral elements as a vector virus or viral particle as vehicle, e.g. encapsulating small organic molecule

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  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

“partícula similar a vírus, ácido nucleico e métodos para produzir rna de fita dupla e sirna, shrna, sshrna, 1shrna e mirna” resumo são descritos processos e composições inovadores que utilizam proteínas capsídeas virais resistentes a hidrolases para preparar partículas similares a vírus a fim de encerrar e, mais tarde, isolar e purificar moléculas carregadas-alvo de interesse, incluindo ácidos nucleicos, tais como sirnas, shrnas, mirnas, rnas mensageiros, peptídeos pequenos e moléculas bioativas. 97/1 1/1
BR112015029559A 2013-06-19 2014-06-05 partícula similar a vírus, ácido nucleico e métodos para produzir rna de fita dupla e sirna, shrna, sshrna, 1shrna e mirna BR112015029559A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361836833P 2013-06-19 2013-06-19
US201361838736P 2013-06-24 2013-06-24
US201361857965P 2013-07-24 2013-07-24
PCT/US2014/041111 WO2014204667A1 (en) 2013-06-19 2014-06-05 Compositions and methods using capsids resistant to hydrolases

Publications (1)

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BR112015029559A2 true BR112015029559A2 (pt) 2017-12-12

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Country Status (15)

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US (2) US9822361B2 (pt)
EP (3) EP3789017A1 (pt)
JP (1) JP6636418B2 (pt)
AU (1) AU2014281061B2 (pt)
BR (1) BR112015029559A2 (pt)
CA (1) CA2912131C (pt)
CY (1) CY1124583T1 (pt)
DK (1) DK3222274T3 (pt)
ES (1) ES2847151T3 (pt)
HU (1) HUE053033T2 (pt)
IL (1) IL242487B (pt)
MX (2) MX364809B (pt)
PL (1) PL3222274T3 (pt)
SG (2) SG11201510181YA (pt)
WO (1) WO2014204667A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR089440A1 (es) * 2011-12-21 2014-08-20 Apse Llc Procesos que emplean particulas similares a virus (psv) con capsides resistentes a hidrolasas
HUE053033T2 (hu) 2013-06-19 2021-06-28 Apse Llc Hidrolázoknak ellenálló kapszidokat alkalmazó készítmények és eljárások
US9932563B2 (en) * 2013-09-11 2018-04-03 Georgia Tech Research Corporation Compositions and methods for inhibiting gene expressions
EP3294276A4 (en) * 2015-05-12 2018-10-31 Apse, Llc In vivo production of long double stranded rna utilizing vlps
EP3356531A4 (en) * 2015-10-01 2019-05-29 Apse Llc METHODS AND COMPOSITIONS COMPRISING APSE NODES
JP7140386B2 (ja) * 2016-03-15 2022-09-21 アプス， インコーポレイテッド 増加した二本鎖ｒｎａ産生のための方法及び組成物
US10131911B2 (en) 2016-11-07 2018-11-20 nanoSUR LLC Post-transcriptionally chemically modified double strand RNAs
WO2020254876A1 (en) 2019-06-18 2020-12-24 Janssen Sciences Ireland Unlimited Company Virus-like particle delivery of hepatitis b virus (hbv) vaccines
CN118302524A (zh) 2021-08-31 2024-07-05 纳诺索尔公司 高分子量修饰的dsRNA组合物

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5443969A (en) 1992-10-29 1995-08-22 Rutgers University RNA packaging system
US6066318A (en) 1995-10-05 2000-05-23 G.D. Searle & Co. Multi-functional hematopoietic fusion proteins between sequence rearranged C-MPL receptor agonists and other hematopoietic factors
US6232287B1 (en) * 1998-03-13 2001-05-15 The Burnham Institute Molecules that home to various selected organs or tissues
GB9925459D0 (en) * 1999-10-27 1999-12-29 Plant Bioscience Ltd Gene silencing
GB9927444D0 (en) * 1999-11-19 2000-01-19 Cancer Res Campaign Tech Inhibiting gene expression
KR101215789B1 (ko) * 2000-03-30 2012-12-26 화이트헤드 인스티튜트 포 바이오메디칼 리서치 Ｒｎａ 간섭의 ｒｎａ 서열 특이적인 매개체
CH695778A5 (de) * 2000-11-29 2006-08-31 Hoffmann La Roche Hemmung der Transkription eines Zielgens in einer Zelle oder in Geweben.
US7320793B2 (en) 2001-01-19 2008-01-22 Cytos Biotechnology Ag Molecular antigen array
DE60233061D1 (de) 2001-09-06 2009-09-03 Alphavax Inc Alphavirus replikon-vektorsysteme
WO2003024481A2 (en) 2001-09-14 2003-03-27 Cytos Biotechnology Ag Packaging of immunostimulatory substances into virus-like particles: method of preparation and use
IN2008CN05409A (pt) 2002-07-18 2015-10-02 Cytos Biotechnology Ag
WO2005001039A2 (en) * 2003-05-29 2005-01-06 Creighton University Ribozyme-regulated small inhibitory rna (sirna) production and methods of use thereof
JP2007528727A (ja) * 2004-02-27 2007-10-18 ザ　ダウ　ケミカル　カンパニー 植物細胞における高効率ペプチド生産
GR20060100496A (el) * 2006-09-01 2008-04-15 Παρθενιος Μπουλικας ΛΙΠΟΣΩΜΑΤΙΚΑ ΕΓΚΑΨΥΛΙΩΜΕΝΟΙ ΦΟΡΕΙΣ-ΥΒΡΙΔΙΟΥ ΑΔΕΝΟΙΟΥ - ΙΟΥ SEMLIKI FOREST (SFV), ΠΟΥ ΦΕΡΕΙ ΚΑΤΑΣΚΕΥΑΣΜΑΤΑ RNAi ΚΑΙ ΘΕΡΑΠΕΥΤΙΚΑ ΓΟΝΙΔΙΑ ΠΟΥ ΧΡΗΣΙΜΟΠΟΙΟΥΝΤΑΙ ΕΝΑΝΤΙΟΝ ΣΤΟΧΩΝ ΚΑΡΚΙΝΟΥ ΚΑΙ ΑΛΛΩΝ ΑΣΘΕΝΕΙΩΝ
US20080171361A1 (en) * 2007-01-11 2008-07-17 The Board Of Regents For Oklahoma State University CAP-Independent Translational Enhancer for Protein Synthesis in Wheat Germ Extract and Transgenic Cereals
CN101784655A (zh) 2007-04-27 2010-07-21 菲尼克斯股份有限公司 可溶性重组二十面体病毒样颗粒的改良生成和体内装配
US20110111496A1 (en) * 2007-06-29 2011-05-12 Chiang Li BACTERIA-MEDIATED GENE MODULATION VIA microRNA MACHINERY
EP2085479A1 (en) 2008-01-31 2009-08-05 Institut Pasteur Reverse genetics of negative-strand rna viruses in yeast
US20110250675A1 (en) 2008-03-26 2011-10-13 Life Technologies Corporaiton Virus-Like Particle Mediated Cellular Delivery
US8324149B2 (en) 2008-11-18 2012-12-04 The Board Of Trustees Of The Leland Stanford Junior University Encapsidation of heterologous entities into virus-like particles
US8906863B2 (en) * 2009-02-27 2014-12-09 The Regents Of The University Of California Proteolysis-resistant capsid of chimeric hepatitis E virus as an oral delivery vector
US8906862B2 (en) 2009-02-27 2014-12-09 The Regents Of The University Of California Multiple antigen delivery system using hepatitis E virus-like particle
US20120174263A1 (en) * 2009-06-15 2012-07-05 Plant Bioscience Limited Production of viral capsids
US20130047297A1 (en) 2010-03-08 2013-02-21 Robert D. Sammons Polynucleotide molecules for gene regulation in plants
WO2011116226A2 (en) 2010-03-17 2011-09-22 Stc.Unm Display of antibody fragments on virus-like particles of rna bacteriophages
WO2012051152A2 (en) * 2010-10-12 2012-04-19 Kevin Ryan Novel dna templates for small rna production in mammalian cells
US20130224828A1 (en) * 2010-11-01 2013-08-29 Qapsule Technologies, Inc. RNA-Directed Packaging of Enzymes Within Protein Particles
JP2013018754A (ja) * 2011-07-13 2013-01-31 Univ Of Tokushima 悪性胸膜中皮腫治療剤
WO2013065791A1 (ja) * 2011-11-02 2013-05-10 公立大学法人大阪市立大学 遺伝子発現抑制用二本鎖核酸分子
AR089440A1 (es) 2011-12-21 2014-08-20 Apse Llc Procesos que emplean particulas similares a virus (psv) con capsides resistentes a hidrolasas
HUE053033T2 (hu) 2013-06-19 2021-06-28 Apse Llc Hidrolázoknak ellenálló kapszidokat alkalmazó készítmények és eljárások
US9932563B2 (en) * 2013-09-11 2018-04-03 Georgia Tech Research Corporation Compositions and methods for inhibiting gene expressions

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DK3222274T3 (da) 2020-11-02
EP3222274B1 (en) 2020-10-21
CY1124583T1 (el) 2022-03-24
CA2912131A1 (en) 2014-12-24
EP3010494B1 (en) 2018-08-15
SG10201805197VA (en) 2018-07-30
WO2014204667A1 (en) 2014-12-24
US9822361B2 (en) 2017-11-21
EP3010494A4 (en) 2017-06-07
MX2019005298A (es) 2019-08-12
HUE053033T2 (hu) 2021-06-28
SG11201510181YA (en) 2016-01-28
MX364809B (es) 2019-05-07
US20160177299A1 (en) 2016-06-23
JP6636418B2 (ja) 2020-01-29
US10428329B2 (en) 2019-10-01
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