BR112015019461A2 - método e sistema para determinação de uma resposta biológica de um alvo a uma substância solúvel candidata - Google Patents

método e sistema para determinação de uma resposta biológica de um alvo a uma substância solúvel candidata

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Publication number
BR112015019461A2
BR112015019461A2 BR112015019461A BR112015019461A BR112015019461A2 BR 112015019461 A2 BR112015019461 A2 BR 112015019461A2 BR 112015019461 A BR112015019461 A BR 112015019461A BR 112015019461 A BR112015019461 A BR 112015019461A BR 112015019461 A2 BR112015019461 A2 BR 112015019461A2
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BR
Brazil
Prior art keywords
concentration profile
candidate
target
biological response
determining
Prior art date
Application number
BR112015019461A
Other languages
English (en)
Inventor
Werner Michael
E Martin Rainer
Anton Hochstrasser Remo
Original Assignee
Hoffmann La Roche
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Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112015019461A2 publication Critical patent/BR112015019461A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0694—Creating chemical gradients in a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0472—Diffusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25625—Dilution
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

resumo patente de invenção: "método e sistema para determinação de uma resposta biológica de um alvo a uma substância solúvel candidata". a presente invenção refere-se a um método para a determinação de uma resposta biológica de um alvo (41, 42) a uma substância solúvel candidata que compreende as etapas de: introdução de uma substância solúvel candidata em um fluxo laminar de um líquido tampão (2) para formar um soluto de substância candidata (3), tendo um perfil de concentração inicial (31); dispersão do perfil de concentração inicial (31) para formar um perfil de concentração disperso (32); direção do perfil de concentração disperso (32) em um canal de detecção (12) para formar um perfil de concentração simétrico final (33) no mesmo; introdução de um alvo no canal de detecção (12) para obter um perfil de concentração combinado compreendendo um perfil de concentração alvo constante sobrepondo o perfil de concentração simétrico final (33); contenção no canal de detecção (12) de pelo menos metade do perfil de concentração combinado; e varredura, opticamente, do perfil de concentração combinado para detectar um sinal óptico representativo da resposta biológica do alvo à substância solúvel candidata.
BR112015019461A 2013-03-05 2014-03-04 método e sistema para determinação de uma resposta biológica de um alvo a uma substância solúvel candidata BR112015019461A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13157877 2013-03-05
PCT/EP2014/054117 WO2014135512A1 (en) 2013-03-05 2014-03-04 Method and system for determining a biological response of a target to a soluble candidate substance

Publications (1)

Publication Number Publication Date
BR112015019461A2 true BR112015019461A2 (pt) 2017-07-18

Family

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Family Applications (1)

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BR112015019461A BR112015019461A2 (pt) 2013-03-05 2014-03-04 método e sistema para determinação de uma resposta biológica de um alvo a uma substância solúvel candidata

Country Status (12)

Country Link
US (1) US9797891B2 (pt)
EP (1) EP2965082B1 (pt)
JP (1) JP6403074B2 (pt)
KR (1) KR20150125000A (pt)
CN (1) CN105264378B (pt)
BR (1) BR112015019461A2 (pt)
CA (1) CA2902786A1 (pt)
ES (1) ES2617681T3 (pt)
HK (1) HK1213995A1 (pt)
MX (1) MX358153B (pt)
RU (1) RU2671974C2 (pt)
WO (1) WO2014135512A1 (pt)

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Publication number Priority date Publication date Assignee Title
FR3065885B1 (fr) * 2017-05-03 2021-12-17 Commissariat Energie Atomique Systeme de melange microfluidique comprenant un injecteur commande pour effectuer un melange avec une dispersion de type taylor aris
CN109799220B (zh) * 2018-12-21 2021-03-26 中国科学院合肥物质科学研究院 基于金属螯合物拉曼标签技术检测组织液中组胺的方法
US20230132619A1 (en) 2020-03-11 2023-05-04 Fida Biosystems Aps A method, an apparatus, an assembly and a system suitable for determining a characteristic property of a molecular interaction
CN112473757B (zh) * 2020-11-19 2021-12-17 江南大学 一种用于食品安全快速检测的微流控芯片检测系统

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2252256C2 (ru) * 1995-09-06 2005-05-20 Айкос Корпорейшн ГОМОЛОГИ (ATR) ПОЛИПЕПТИДА rad3, ПОЛИНУКЛЕОТИДЫ И СВЯЗАННЫЕ С НИМИ СПОСОБЫ И МАТЕРИАЛЫ
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
US20040053315A1 (en) * 2002-08-12 2004-03-18 Caliper Technologies Corp. Methods and systems for monitoring molecular interactions
WO2004059299A1 (en) * 2002-12-16 2004-07-15 Cytodiscovery, Inc. Microfluidic system with integrated permeable membrane
WO2005033672A1 (en) * 2003-10-01 2005-04-14 Caliper Life Sciences, Inc. Method for measuring diffusivities of compounds using microfluidic devices
TW200537094A (en) * 2004-05-11 2005-11-16 Shi-Ping Liu Gaseous chemical biological exposure chamber
JP2007044041A (ja) * 2005-07-13 2007-02-22 Mitsubishi Chemicals Corp Ｓｃｒａｐｐｅｒタンパク質を利用した医薬候補物質のスクリーニング方法
JP4273425B2 (ja) * 2005-09-28 2009-06-03 独立行政法人産業技術総合研究所 マイクロ流路利用分子分析方法
JP2007268491A (ja) * 2006-03-31 2007-10-18 Fujifilm Corp マイクロデバイス及びそれを用いた触媒反応方法
KR100940598B1 (ko) * 2007-10-12 2010-02-04 부산대학교 산학협력단 Ｄｎａ검출용 마이크로칩 및 이를 이용한 검출방법
US7813882B2 (en) 2008-02-29 2010-10-12 Wyatt Technology Corporation Method for determining average properties of molecules in solution
WO2011042509A2 (en) * 2009-10-08 2011-04-14 Universite De Strasbourg Apparatus and processes for generating variable concentration of solutes in microdroplets
JP5881031B2 (ja) * 2010-03-18 2016-03-09 国立研究開発法人産業技術総合研究所 薬剤感受性試験用バイオチップ
SG10201508047SA (en) * 2010-09-29 2015-10-29 Massachusetts Inst Technology Device for high throughput investigations of cellular interactions
US20130273564A1 (en) * 2010-12-23 2013-10-17 John Gerard Quinn Dispersion injection methods for biosensing
FR2972117B1 (fr) * 2011-03-04 2013-12-20 Centre Nat Rech Scient Systeme microfluidique pour controler un profil de concentration de molecules susceptibles de stimuler une cible
CN102586394B (zh) * 2012-02-28 2014-01-01 中国科学院微生物研究所 一种筛选以神经酰胺糖脂为作用靶标的抗真菌物质的方法

Also Published As

Publication number Publication date
US9797891B2 (en) 2017-10-24
RU2671974C2 (ru) 2018-11-08
EP2965082A1 (en) 2016-01-13
MX358153B (es) 2018-08-07
HK1213995A1 (zh) 2016-07-15
KR20150125000A (ko) 2015-11-06
ES2617681T3 (es) 2017-06-19
CA2902786A1 (en) 2014-09-12
CN105264378B (zh) 2017-05-24
JP2016509233A (ja) 2016-03-24
RU2015139400A (ru) 2017-04-07
CN105264378A (zh) 2016-01-20
WO2014135512A1 (en) 2014-09-12
MX2015011369A (es) 2015-12-16
JP6403074B2 (ja) 2018-10-10
US20160011180A1 (en) 2016-01-14
EP2965082B1 (en) 2016-12-28

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Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2661 DE 04-01-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.