Claims (3)
200537094 拾、申請專利範圍·· f發^申％專利顧為醫藥之毒性物質評估與藥物治療方面。 1·醫樂之毒性物質評估··姻本發啊提供醫藥研究人員氣體化學 物質對於實驗動物活體之短、中、細，依據活體動物之身體反 應、錢變化、細胞畸形與器官衰退情形，未來可做為保障民眾在就 業時之作業環境規範，避免職業病之發生。 2·醫藥之藥物治療評估：由於氣喘和職多半由空齡之過敏原造成， 呼吸性藥物治療可顯著提升患者之治瘡機率，本發明亦可使用治療性 氣體或微粒藥物，通過實驗動物之呼吸器官進入動物體，進而評^目 標藥物對於動物體之生理影響與治癒病症評估。 口200537094 Scope of patent application, f ...% of patents filed for the evaluation of toxic substances in medicine and drug treatment. 1. Evaluation of Toxic Substances in Medical Music. We provide medical researchers with short, medium, and fine gas chemical substances for living animals, based on the body's physical response, changes in money, cell deformities, and organ decline in the future. It can be used to protect the working environment of the people when they are employed and to prevent occupational diseases. 2 · Medicine drug treatment evaluation: Asthma and occupation are mostly caused by empty-age allergens. Respiratory drug treatment can significantly increase the chance of treating patients with sores. The present invention can also use therapeutic gas or particulate drugs. Respiratory organs enter the animal body, and further evaluate the physiological effects of the target drug on the animal body and the evaluation of the cured disease. mouth
9 200537094 柒、指定代表圖： (一） 本案指定代表圖為：第（1 )圖。 (二) 本代表圖之元件代表符號簡單說明: ① 化學性氣體生物暴露箱（三組） ② 化學性物質氣化裝置（平行兩組） ③ 抽氣通風系統與活性碳吸附設備9 200537094 (1) Designated representative map: (1) The designated representative map in this case is: (1). (2) Brief description of the component representative symbols of this representative map: ① Chemical gas biological exposure box (three groups) ② Chemical substance gasification device (parallel two groups) ③ Extraction ventilation system and activated carbon adsorption equipment
捌、本案若有化學式時，請揭示最能顯示發明特徵的化學式： (本說明書格式、順序及粗體字，請勿任意更動，※記號部分請勿填寫） ※申請案Λ : 093113281 q ※申請日期：93年5月日 分類：6。丨^% 壹、發明名稱：（中文/英文) 化學氣體生物暴露箱/ Gaseous Chemical Biological Exposure Chamber 贰、申請人：（共1人） 姓名或名稱：（中文/英文）（簽章）ID :D120119237 劉希平/Liu，Shi-Ping 代表人：（中文/英文）（簽章）劉希平/Liu，Shi-Ping 住居所成營業所地址：（中文/英文） 台北縣永和市福和路309巷20弄11號5樓 國籍·（中文/英文）中華民國/Republic of China 電話/傳真/手機·· 02-2925-6090 (FAX) 02-2901-7016 E-MAIL ： phl005@mails.§u.edu.tw 參、發明人··（共1人） 姓名：（中文/英文） ID ： D120119237 劉希平/Liu，Shi-Ping 住居所地址：（中文/英文） 台北縣永和市福和路309巷20弄11號5樓 國籍·（中文/央文）中華民國/RepUbiic 〇f china .200537094 肆、聲明事項： 本案係符合專利法第二十條第一項□第一款但書或□第二款但書規定之期 間，其日期為：年月曰。 ◎本案申請前已向下列國家（地區）申請專利□主張國際優先權： 【格式請依：受理國家（地區）；申請日；申請案號數順序註記】 1. 2. 3. 4. 5. □主張國内優先權（專利法第二十五條之一）： 【格式請依：申請曰；申請案號數順序註記】 1. 2. □主張專利法第二十六條微生物： □國内微生物【格式請依：寄存機構；日期；號碼順序註記】 □國外微生物【格式請依··寄存國名；機構；日期；號碼順序註記】 □熟習該項技術者易於獲得，不須寄存 200537094 柒、 指定代表國： 無 捌、 本案若有化學式時，請揭示最艇顯示發明特徵的化學式： 無 玖、發明說明： 【發明所屬之技術領域】本發明可應用在生物醫學領域之化學物質毒 性生物活體暴露（invivo)試驗，評估化學性物質對於生物體之長期健 康效應，同時本發明亦可應用於呼吸性藥物對於生物體之治療效果。 【先前技術】傳統呼吸暴露裝置是使用狹窄侷限之裝置，限制實驗鼠 之活動和飲食，再以高濃度之污染氣體強迫實驗鼠以口鼻呼吸，經由 數小時、長則一天之暴露時間後，隨之進行動物生理測試或解剖，摘 取血液檢體和目標器官，進行後續醫學研究之用，藉以判斷污染物質 和組織之病變相關性，此即為活體暴露（invivo)之傳統進行方式。但 是此種方式缺點為高濃度、短時間之暴露，與一般造成疾病之低濃度 長時間因素不同，再加以實驗鼠侷限活動和限制飲食，均與一般生物 活動型態不同，其真實活體暴露結果受到相當程度批評，其醫學應用 亦有多方面限制。。 【發明内容】本發明應用通風技術與化學性氣體曝氣裝置，供應長期 中、低濃度之化學性氣體，使在暴露箱内之實驗動物長期穩定地進行 活體生物實驗，模擬一般實驗動物正常活動情形。實驗動物除了可自 由進食與生理排泄外，配合不同濃度之暴露條件，並可由透明外殼， 觀察實驗動物在暴露實驗過程中之即時生理反應，評估實驗鼠對於不 同氣態化學物質之毒性效應與生理反應，作為目標化學性氣體對於生 200537094 物體產生毒性或醫療效果之探討，未來更可應用此暴露箱設備研發治 療呼吸性疾病藥物之有效性與應用性。 σ 【實施方式】 本發明則依據氣體蒸發裝置、室内通風系統與排氣污染防制技術， 设計控制穩定之氣體暴露箱，提供污染物質給予實驗生物體呼吸，藉 以評估污染物質危害健康情形；或以相同之暴露設備提供呼吸性治療 藥物，治療呼吸系統之相關疾病。本發明研發過程曾將實驗鼠至入暴 露箱中長達三個月，在一般空氣情形下，實驗鼠之體重增加情形與室 外對照組實驗鼠並無顯著差異，顯示本暴露箱之設計通風及實驗鼠活 動成長情形均可接受。 本發明主要技術在於提供低污染物質濃度給予實驗鼠長期自由活 ，下之呼吸暴露裝置，污染物質濃度可藉由氣體化合物注入裝置和空 氣通風系統持績注入暴露箱，實驗鼠可自由地在透明暴露箱中飲食與 /舌動’研究人員可以在外界直接觀察實驗鼠在暴露過程中之活動變 化’並可依據暴露實驗需求，取㈣驗鼠進行血液或其他生理解剖研 究。污染物質濃度可以控制在接近一般職業環境之濃度，暴 露時間可 以長達數個月，此發明除了可以評估污染物質對於健康之危害外，更 可用來評獅吸性藥物經由呼吸系統治療之活體功效。 200537094 【圖式簡單說明】 (一） 化學氣體生物暴露箱全圖 (二） 暴露箱之立體、上視、正視及側視圖 (三） 兩組化學性物質飽和氣化產生裝置（目的為產生兩種不同流量 之飽和化學性氣體） (四） 本發明之暴露實驗實景 【元件代表符號說明】 ① 化學性氣體生物暴露箱（對照組）：一組透明密閉性之生物暴露 箱，可容納實驗鼠或兔進行活體暴露研究，實驗動物可自由活 動、呼吸、進食與排泄，真正模擬動物在一般環境之生活狀況。 對照組是以自然空氣為主，作為判別生物暴露在一般環境下之結 果，作為化學氣體生物暴露之比較。 ② 化學性氣體生物暴露箱（實驗組）：兩組與對照組生物暴露箱之 相同設計，但供應不同流量之化學飽和氣體，注入兩個實驗組暴 露箱，藉以評估不同化學氣體濃度對於實驗生物體在不同暴露時 期造成之影響。 ③ 化學性物質飽和氣化裝置：飽和氣化裝置為提供目標化學性物 質，以曝氣方式將液態化學物質達到飽和氣化程度，穩定注入上 述之實驗組暴露箱，並可控制預期氣體濃度，達到長期生物活體 暴露之目的。飽和氣化裝置採用兩組平行進行，產生兩種不同流 量之飽和氣體，分別提供實驗組暴露箱不同化學性氣體濃度，進 行活艘暴露實驗。 ④ 通風抽氣幫浦·獨立抽氣幫浦提供每一個密閉暴露箱通風空氣之 用，抽氣幫浦必須依據暴露生物體體重和數量進行空氣流量之調 整，以提供足夠空氣量給予生物暴露箱内之生物體。藉由抽氣幫 浦之空氣流量控制，配合化學性物質飽和氣體流量，可調整生物 暴露箱内之氣體化學物質濃度。 200537094 ⑤ 活性碳吸附設備··而在抽氣幫浦排出污染氣體前，以活性 裝置去除潛在污染環境之有害臭味與化學物質。 吸附 ⑥ 氮氣氣體鋼瓶：提供氮氣氣體來源。 ⑦ 拉門：採上下活動式設計，目的在於便利實驗動物籠之進 是需符合密閉之要求。 ，值 ⑧ 空氣進氣口 ··暴露箱之新鮮空氣入口。 ⑨ 上方出氣裝置··暴露箱内出氣裝置位於暴露箱上方，新鮮空々々 合飽和氣化裝置（③）產生之化學性氣體，經由此域裝^^ 進入暴露箱箱内。 句勻 ⑭If排氣裝置··排氣裝置在暴露箱下方角落，穩定排出箱内污染 空氣。 ⑪廢氣排氣口 ··以抽氣幫浦（④）經由此排氣口，將暴露箱内之卜 染空氣抽出，並達到暴露箱内空氣循環之目的。 巧 ⑫風扇：藉由暴露箱頂端兩個循環風扇，使暴露箱内空氣與化 氣體均勻分布各角落。 ⑬實驗動物籠進入方向··將拉門（⑦）向上打開，實驗動物籠（⑲） 可依照箭頭方向放入暴露箱，而後將拉門放下，確實密閉，以 有機氣體外洩。 # ⑭曝氣瓶：三個連續曝氣瓶，内裝盛液態化學藥劑，依序導入特定 流量之氮氣氣體，以定量曝氣的方式使液態化學藥劑氣化成為 和蒸汽。 ~ ⑮浮子流量計：氮氣氣體通過密閉玻璃管柱時，向上推動浮子圓 球，藉以顯示氮氣氣體之流量。 ⑩流量調整閥：附屬於浮子流量計之針閥，用來控制氮氣氣體的流 量，以產生飽和蒸汽的化學氣體。 ⑫氮氣入口端·氮氣氣體之進入位置。 ⑩化學性氣體出氣端：經由曝氣瓶（⑭）所產生的飽和化學氣體由 此端流出，導入實驗組暴露箱（②）中。 ⑲實驗動物籠··一般醫學研究使用之實驗動物籠，上方可供應食物 200537094 與飲水，實驗鼠籠下方放置木屑，以吸收實驗鼠之尿與糞便，減 少實驗過程之異味。 ⑳實驗鼠：暴露實驗之目標生物活動處。 ㉑食物放置處：提供實驗動物食用。 ㉒飲用水瓶：提供實驗動物飲水。 •200537094 伍、中文發明摘要： 本發明之主要特點為一套可精密控制氣體濃度之動物暴露箱，進行 短、中、長期之實驗鼠活體暴露實驗，提供醫藥或危害健康物質在特定濃 度下，對於動物活體之健康影響評估。在此暴露箱中，動物鼠可在接近於 一般環境自由生活，無拘束地呼吸配製特定濃度之氣體，以進行活體暴露 實驗。實驗鼠於密閉透明箱内進行短則數小時或數天、長則數周或數月之 暴露實驗和隨後之醫學生理研究，用來評估實驗鼠對於環境中目標氣體之 活體暴露生理反應和致癌制，未來可應麟氣喘病患之氣體藥物治療研 究或工作環境中有害氣體濃度對動物或人體造成之生理效應，提供醫藥研 究及勞工作業環境訂定適當法規和容許標準。 本設計主要部分包括： 1·三組獨立賴通風之透明暴組暴露箱為職組；而其餘兩組為 不同化學氣體濃度之實驗組。每峰露細有獨立空調系統，並可容納 -具實驗鼠ϋ和長時間監測有機溶劑濃度活體暴露系統。暴露箱内裝設 目標氣體注人纽、兩減賴散風扇和㈣減錄等，翻外麵 可模擬日夜間變化之實驗需求，提供研究者觀察動物活動暴露實驗情形。 2. 化學性物質氣化裝置：糊缝鋼瓶高魏氣、流餘㈣統和密閉有 機溶劑曝氣纽，提個歧量的特定有機溶麵和魏至暴露箱。 3. 抽氣线和職概附裝置：提供做風紅轉収和處理排放廢氣 之活性碳吸附設備，減少實驗排氣污染對於周圍環境之危害。 .200537094 陸、英文發明摘要：捌. If there is a chemical formula in this case, please disclose the chemical formula that best shows the features of the invention: (This manual is in the format, order and bold type, please do not change it arbitrarily. ※ Please fill in the marked part.) ※ Application Λ: 093113281 q ※ Application Date: May 93rd Category: 6.丨 ^% I. Invention Name: (Chinese / English) Chemical Gas Biological Exposure Chamber / Gaseous Chemical Biological Exposure Chamber 申请人, Applicant: (1 person in total) Name or Name: (Chinese / English) (Signature) ID: D120119237 Liu Xiping / Liu, Shi-Ping Representative: (Chinese / English) (Signature) Liu Xiping / Liu, Shi-Ping Residence Address: (Chinese / English) Lane 11, Lane 309, Fuhe Road, Yonghe City, Taipei County No. 5 Nationality · (Chinese / English) Republic of China / Republic of China Phone / Fax / Mobile ·· 02-2925-6090 (FAX) 02-2901-7016 E-MAIL: phl005@mails.§u.edu.tw Participants and inventors (1 person) Name: (Chinese / English) ID: D120119237 Liu Xiping / Liu, Shi-Ping Address: (Chinese / English) No. 11, Lane 20, Lane 309, Fuhe Road, Yonghe City, Taipei County Nationality on the 5th floor · (Chinese / Central Chinese) Republic of China / RepUbiic 〇f china .200537094 Information and declaration matters: This case complies with Article 20 (1) of the Patent Law □ Paragraph 1 or □ Paragraph 2 During this period, its date is: year, month, and year. ◎ Before the application of this case, patents have been applied to the following countries (regions) □ Claiming international priority: [format please follow: receiving country (region); application date; application note number sequence notes] 1. 2. 3. 4. 5. □ Claim domestic priority (One of Article 25 of the Patent Law): [Please follow the format: Application; Note on the order of the application number] 1. 2. □ Claim Article 26 of the Patent Law Microorganism: □ Country Internal microorganisms [format please follow: deposit institution; date; number sequence notes] □ Foreign microorganisms [format please follow · · deposit country name; institution; date; number sequence notes] □ Those who are familiar with this technology are easy to obtain and do not need to deposit 200537094 柒, Designated representative country: No 捌, if there is a chemical formula in this case, please disclose the chemical formula showing the characteristics of the invention: No 玖, Description of the invention: [Technical field to which the invention belongs] The present invention can be applied to the chemical substances in the field of biomedicine Toxic biological in vivo test to evaluate the long-term health effects of chemical substances on organisms, and the invention can also be applied to respiratory drugs In the treatment of the organism. [Previous technology] The traditional respiratory exposure device uses a narrow and confined device to restrict the movement and diet of experimental rats, and then forces the experimental rats to breathe through the nose and nose with a high concentration of polluting gas. After several hours and a day of exposure, Animal physiological tests or dissections are subsequently performed, blood specimens and target organs are removed, and subsequent medical research is performed to determine the correlation between the contaminated substances and tissue lesions. This is the traditional method of invivo exposure. However, the shortcomings of this method are high concentration and short-term exposure, which is different from the low concentration and long-term factors that generally cause disease. In addition, the experimental mice's restricted activities and dietary restrictions are different from the general biological activity types, and the actual live exposure results It has been criticized to a considerable degree, and its medical application has many limitations. . [Summary of the invention] The present invention applies ventilation technology and chemical gas aeration device to supply long-term medium and low-concentration chemical gas, so that the experimental animals in the exposure box can perform live biological experiments stably for a long time, and simulate the normal activities of general experimental animals. situation. In addition to free feeding and physiological excretion, experimental animals can be exposed to different concentrations of exposure conditions, and the transparent shell can be used to observe the immediate physiological responses of experimental animals during the exposure experiment, and to evaluate the toxic effects and physiological responses of experimental mice to different gaseous chemical substances. As a discussion of the target chemical gas's toxicity or medical effect on raw 200537094 objects, the effectiveness and applicability of this exposure box equipment in the development of drugs for the treatment of respiratory diseases can be applied in the future. σ [Embodiment] The present invention is based on a gas evaporation device, an indoor ventilation system, and an exhaust pollution prevention technology to design and control a stable gas exposure box to provide pollutants to the experimental organisms to breathe, thereby assessing the health situation of the pollutants; Or use the same exposure equipment to provide respiratory therapy drugs to treat related diseases of the respiratory system. During the research and development process of the present invention, the experimental rats were put into the exposure box for up to three months. Under normal air conditions, the weight gain of the experimental rats was not significantly different from that of the outdoor control group. Rat growth was acceptable. The main technology of the present invention is to provide a low-pollution substance concentration to give experimental rats a long-term freedom of living, and a breathing exposure device. The concentration of the pollutant substance can be injected into the exposure box through the gas compound injection device and the air ventilation system. The experimental mice can be freely transparent. Diet and / or tongue movement in the exposure box 'Researchers can directly observe the changes in the activities of experimental rats during the exposure process' and can take test mice for blood or other physiological anatomy studies according to the requirements of the exposure experiment. The concentration of pollutants can be controlled close to the general occupational environment, and the exposure time can be up to several months. In addition to assessing the health hazards of pollutants, this invention can also be used to evaluate the efficacy of lion inhaled drugs through the respiratory system . 200537094 [Schematic description] (1) Full view of chemical gas biological exposure box (2) Three-dimensional, top view, front view, and side view of the exposure box (3) Two sets of chemical substance saturation gasification generating devices (the purpose is to generate two (Saturated chemical gas with different flow rates) (4) The actual exposure experiment of the present invention [Explanation of the representative symbols of the components] ① Chemical gas biological exposure box (control group): a set of transparent and sealed biological exposure boxes that can accommodate experimental rats Or rabbits are subjected to in vivo exposure studies. The experimental animals can move, breathe, eat and excrete freely, and truly simulate the animal's living conditions in the general environment. The control group was based on natural air as the result of judging the biological exposure to the general environment and the comparison of chemical gas biological exposure. ② Chemical gas biological exposure box (experimental group): The two groups have the same design as the biological exposure box of the control group, but supply different volumes of chemically saturated gas and inject them into the two experimental group exposure boxes to evaluate the concentration of different chemical gases for the experimental organisms. The effects of the body in different periods of exposure. ③ Saturated gasification device for chemical substances: To provide the target chemical substance, the saturated gasification device will saturate the liquid chemical substance to a degree of saturation by aeration, stably inject it into the above-mentioned experimental group exposure box, and control the expected gas concentration. To achieve the purpose of long-term biological living exposure. The saturated gasification device uses two sets of parallel processes to generate two kinds of saturated gases with different flows, and provides different chemical gas concentrations in the exposure box of the experimental group for live boat exposure experiments. ④ Ventilation pumps · Independent pumps provide ventilation for each closed exposure box. The pumps must adjust the air flow according to the weight and quantity of exposed organisms to provide sufficient air for the biological exposure box. The organism inside. Through the air flow control of the pumping pump and the saturated gas flow of the chemical substance, the concentration of the gas chemical substance in the biological exposure box can be adjusted. 200537094 ⑤ Activated carbon adsorption equipment ... Before the exhaust pump exhausts polluted gas, remove the harmful odor and chemical substances that potentially pollute the environment with an activated device. Adsorption ⑥ Nitrogen gas cylinder: Provide nitrogen gas source. ⑦ Sliding door: It is designed to be movable up and down. The purpose is to facilitate the entrance of experimental animal cages. It must meet the requirements of containment. , Value ⑧ Air inlet · Fresh air inlet of the exposure box.上方 Upper air outlet device ·· The air outlet device in the exposure box is located above the exposure box. The fresh air is mixed with the chemical gas generated by the saturated gasification device (③), and is installed in this area into the exposure box. Sentence ⑭If the exhaust device ·· The exhaust device is in the lower corner of the exposure box to stably discharge the polluted air in the box. ⑪Exhaust gas exhaust port · Exhaust pump (④) through this exhaust port to extract the polluted air in the exposure box, and achieve the purpose of air circulation in the exposure box. Qiao Fan: With two circulating fans at the top of the exposure box, the air and the gas in the exposure box are evenly distributed in all corners.方向 Direction of entering the experimental animal cage ·· Open the sliding door (⑦) upwards. The experimental animal cage (⑲) can be placed in the exposure box in the direction of the arrow, and then the sliding door is lowered, and it is surely sealed, and the organic gas is leaked. # ⑭ Aeration bottles: Three continuous aeration bottles containing liquid chemicals, sequentially introducing nitrogen gas at a specific flow rate, and vaporizing the liquid chemicals into and steam by means of quantitative aeration. ~ ⑮Float flowmeter: When nitrogen gas passes through a closed glass tube column, push the float ball upwards to display the flow rate of nitrogen gas. ⑩Flow adjustment valve: Needle valve attached to the float flow meter, used to control the flow of nitrogen gas to produce saturated steam chemical gas. ⑫Nitrogen inlet and entry position of nitrogen gas. ⑩Chemical gas outlet end: The saturated chemical gas generated through the aeration bottle (⑭) flows out from this end and is introduced into the exposure box (②) of the experimental group. ⑲Experimental animal cages · The experimental animal cages used in general medical research can be supplied with food 200537094 and drinking water. Wood chips are placed under the experimental mouse cages to absorb the urine and feces of the experimental rats and reduce the odor of the experimental process. ⑳Experimental mouse: The target biological activity of the exposure experiment.放置 Food place: Provide experimental animals. ㉒ Drinking water bottle: Provide drinking water for experimental animals. • 200537094 Abstract of Chinese and Chinese inventions: The main feature of the present invention is a set of animal exposure cabinets that can precisely control the gas concentration. It can perform short, medium and long-term experiments on laboratory mouse in vivo exposure experiments, and provide medicines or health-endangering substances at specific concentrations. Assessment of the health effects of live animals. In this exposure box, animals and rats can live freely close to the general environment and breathe freely to prepare a specific concentration of gas for living exposure experiments. Laboratory rats are exposed in a closed transparent box for several hours or days, and long weeks or months, and subsequent medical physiology studies are used to evaluate the physiological response and carcinogenicity of experimental mice to the target gas in the environment. In the future, we can respond to the gas drug treatment research of Lin asthmatic patients or the physiological effects of harmful gas concentrations in the working environment on animals or human bodies, and provide medical research and labor operating environments to set appropriate regulations and allowable standards. The main parts of this design include: 1. Three groups of independent exposure ventilated transparent exposure groups are the working group; the remaining two groups are experimental groups with different chemical gas concentrations. Each peak dew has an independent air-conditioning system, and can accommodate-a living rat exposure system with experimental rat pupa and long-term monitoring of the concentration of organic solvents. The exposure box is equipped with a target gas injection button, two submersible fans, and a submerged recorder, etc. The outside can simulate the experimental requirements of day and night changes, and provide researchers to observe the experimental conditions of animal activity exposure. 2. Chemical substance gasification device: paste seam cylinders with high Wei gas, flow residual system and closed organic solvent aeration button, provide a specific amount of specific organic solution and Wei to the exposure box. 3. Extraction line and auxiliary equipment: Provide activated carbon adsorption equipment that converts waste gas into red and collects and treats exhaust gas, which reduces the harm of experimental exhaust pollution to the surrounding environment. .200537094 Lu and English invention abstracts:
The objective of this invention is to provide constant concentrations of specific gaseous chemicals to experimental rats or mice in transparent plexglass chambers. This invention can be used to investigate short- to long-term in-vivo biological reactions and health effects of specific gaseous chemicals for experimental rats or mice at desired concentrations. In the exposure chambers, experimental rats or mice can live unrestrainedly as they stay in regular environment, while breathing the gaseous chemicals at desired concentrations for in-vivo biological experiments. The experimental period can last hours, days, weeks and even months as long as the rats or mice survive. The applications of the gaseous chemical exposure chambers aids to the understanding of biological response and carcinogenic effect of the target chemicals. This design can be applied to medicine research for asthma patients5 therapy and adverse biological effects and upper concentration limits for target chemicals in working environment. Main design parts of the exposure chamber include: 1. Three independent transparent exposure chambers: One chamber is designed for control group while the other two are for experimental groups at different concentrations. Each chamber equipped with a self ventilation system, which include a gaseous chemical injection system, two circulation fans and an air exhaust system. The exposure chamber accommodates a specific rat cage for rats or mice for a short to long term exposure in-vivo experiment. The transparent character of the exposure chamber is designed for researchers5 observation and of diurnal simulation during the exposure period.The objective of this invention is to provide constant concentrations of specific gaseous chemicals to experimental rats or mice in transparent plexglass chambers. This invention can be used to investigate short-to long-term in-vivo biological reactions and health effects of specific gaseous chemicals for experimental rats or mice at desired concentrations. In the exposure chambers, experimental rats or mice can live unrestrainedly as they stay in regular environment, while breathing the gaseous chemicals at desired concentrations for in-vivo biological experiments. The experimental period can last hours, days , weeks and even months as long as the rats or mice survive. The applications of the gaseous chemical exposure chambers aids to the understanding of biological response and carcinogenic effect of the target chemicals. This design can be applied to medicine research for asthma patients5 therapy and adverse biological effects and upper concentration limits for target chemicals in w orking environment. Main design parts of the exposure chamber include: 1. Three independent transparent exposure chambers: One chamber is designed for control group while the other two are for experimental groups at different concentrations. Each chamber equipped with a self ventilation system, which include a gaseous chemical injection system, two circulation fans and an air exhaust system. The exposure chamber accommodates a specific rat cage for rats or mice for a short to long term exposure in-vivo experiment. The transparent character of the exposure chamber is designed for researchers5 observation and of diurnal simulation during the exposure period.
2. Gaseous chemical generation apparatus: This system provides saturated gaseous chemical at a constant flow of nitrogen gas. The constant nitrogen flow regulated by regulators, valves and rotometers passes through the target pure liquid solvent in specific designed impingers. The saturated chemical gas stream is then dilute into the ventilation system of desired exposure chambers.2. Gaseous chemical generation apparatus: This system provides saturated gaseous chemical at a constant flow of nitrogen gas. The constant nitrogen flow regulated by regulators, valves and rotometers passes through the target pure liquid solvent in specific designed impingers. The saturated chemical gas stream is then dilute into the ventilation system of desired exposure chambers.
3. A pumping system and activated carbon adsorption apparatus: Each chamber equipped with a gas pump operated at desired air flow rates. The activated carbon adsorption apparatus removes gaseous chemical in the exhaust air for minimizing hazard impact to surrounding environment. 4 .200537094 拾、申請專利範固： 本=月申^專利範圍為賴之毒性崎評倾藥物治療方面。 1.醫樂之毋性物質評估：使林發明可提供賴研究人㈣查氣體化學 物質對於實驗動物活體之短、中、長卿響，依據活體動物之身體反 應、血液變化、細胞畸形與器官衰退情形，未來可做為保障民眾在就 業時之作業環境規範，避免職業病之發生。 2·醫藥之藥物治療評估：由於氣喘和過敏多半由空氣中之過敏原造成， 呼吸性藥物治療可顯著提升患者之治癒機率，本發明亦可使用治療性 氣體或微粒藥物，通過實驗動物之呼吸器官進入動物體，進而評估目 標藥物對於動物體之生理影響與治癒病症評估。3. A pumping system and activated carbon adsorption apparatus: Each chamber equipped with a gas pump operated at desired air flow rates. The activated carbon adsorption apparatus removes gaseous chemical in the exhaust air for minimizing hazard impact to surrounding environment. 4.200537094 Application for patent Fangu: This = monthly application ^ The scope of the patent is Lai's toxic drug treatment. 1. Evaluation of non-essential substances in medical music: To enable Lin Invention to provide researchers with short, medium and long-lasting sounds of gas chemical substances in experimental animals, based on the body's physical response, blood changes, cell deformities and organs. In the future, the decline can be used to protect the working environment of the people when they are employed and to prevent occupational diseases. 2. Evaluation of drug treatment of medicine: Asthma and allergies are mostly caused by allergens in the air. Respiratory drug treatment can significantly improve the cure rate of patients. The invention can also use therapeutic gas or particulate drugs to pass the breath of experimental animals. The organ enters the animal body, and then evaluates the physiological effects of the target drug on the animal body and evaluates the cured condition.