BR112015013193A2 - ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, óleo microbiano, e, método para obter óleo microbiano. - Google Patents

ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, óleo microbiano, e, método para obter óleo microbiano.

Info

Publication number
BR112015013193A2
BR112015013193A2 BR112015013193A BR112015013193A BR112015013193A2 BR 112015013193 A2 BR112015013193 A2 BR 112015013193A2 BR 112015013193 A BR112015013193 A BR 112015013193A BR 112015013193 A BR112015013193 A BR 112015013193A BR 112015013193 A2 BR112015013193 A2 BR 112015013193A2
Authority
BR
Brazil
Prior art keywords
microbial oil
obtaining
cell
nucleic acid
expression cassette
Prior art date
Application number
BR112015013193A
Other languages
English (en)
Inventor
Somanchi Aravind
Ewing Aren
Rudenko George
Shoa-Azar Matthew
Bhat Riyaz
Franklin Scott
Brubaker Shane
Original Assignee
Solazyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Solazyme Inc filed Critical Solazyme Inc
Publication of BR112015013193A2 publication Critical patent/BR112015013193A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112015013193A 2012-12-07 2013-12-06 ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, óleo microbiano, e, método para obter óleo microbiano. BR112015013193A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261734613P 2012-12-07 2012-12-07
US201361902705P 2013-11-11 2013-11-11
PCT/US2013/073718 WO2014089514A1 (en) 2012-12-07 2013-12-06 Genetically engineered microbial strains including chlorella protothecoides lipid pathway genes

Publications (1)

Publication Number Publication Date
BR112015013193A2 true BR112015013193A2 (pt) 2017-07-11

Family

ID=50884057

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015013193A BR112015013193A2 (pt) 2012-12-07 2013-12-06 ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, óleo microbiano, e, método para obter óleo microbiano.

Country Status (5)

Country Link
US (1) US9518277B2 (pt)
EP (1) EP2929032A4 (pt)
CN (1) CN104968788A (pt)
BR (1) BR112015013193A2 (pt)
WO (1) WO2014089514A1 (pt)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10415041B2 (en) * 2017-02-09 2019-09-17 Base Pair Biotechnologies, Inc. Functional ligands to target molecules
BR112014012917A2 (pt) 2011-11-28 2017-06-13 Solazyme Inc ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, e, óleo microbiano
WO2014089514A1 (en) 2012-12-07 2014-06-12 Solazyme, Inc. Genetically engineered microbial strains including chlorella protothecoides lipid pathway genes
PL2992020T3 (pl) 2013-05-03 2020-06-01 Ohio State Innovation Foundation Odpornościowe komórki efektorowe wyrażające inżynierowane chimeryczne receptory antygenu swoiste dla CS1
US9969990B2 (en) * 2014-07-10 2018-05-15 Corbion Biotech, Inc. Ketoacyl ACP synthase genes and uses thereof
AU2016219785B2 (en) 2015-02-20 2021-10-28 Ohio State Innovation Foundation Bivalent antibody directed against NKG2D and tumor associated antigens
WO2016139667A1 (en) 2015-03-04 2016-09-09 Ariel-University Research And Development Company Ltd. Peptides and compositions comprising same and uses thereof in the treatment of diseases
EP3277793A1 (en) * 2015-03-31 2018-02-07 TerraVia Holdings, Inc. Microalgae adapted for heterotrophic culture conditions
CN105087629A (zh) * 2015-09-18 2015-11-25 宜春学院 利用小球藻表达人干扰素基因的方法
US10059951B2 (en) * 2016-02-17 2018-08-28 Base Pair Biotechnologies, Inc. Functional ligands to nerve agent metabolites
ES2913257T3 (es) * 2016-04-27 2022-06-01 Univ Osaka Péptido que inhibe la colonización por bacterias patógenas e inhibidor de la colonización que incluye el mismo
US9611297B1 (en) 2016-08-26 2017-04-04 Thrasos Therapeutics Inc. Compositions and methods for the treatment of cast nephropathy and related conditions
US10704066B2 (en) 2017-05-05 2020-07-07 Purissima, Inc. Neurotransmitters and methods of making the same
US20210139920A1 (en) * 2017-08-21 2021-05-13 Indiana University Research And Technology Corporation Solubility enhancing protein expression systems
EP3822289A4 (en) * 2018-07-10 2022-04-13 National University Corporation Kobe University ANTIBODY ANTI-SIRP ALPHA
US11618890B2 (en) 2018-08-22 2023-04-04 Corbion Biotech, Inc. Beta-ketoacyl-ACP synthase II variants
WO2021146520A1 (en) 2020-01-16 2021-07-22 Corbion Biotech, Inc. β-KETOACYL-ACP SYNTHASE IV VARIANTS
CN112813092B (zh) * 2020-08-06 2023-03-21 中国农业科学院棉花研究所 GbBCCP5蛋白质及其编码基因在调控生物油脂含量中的应用
KR102427080B1 (ko) * 2020-08-07 2022-07-29 주식회사 에이엠메딕스 Socs6의 폴리 펩타이드 단편을 포함하는 비만의 개선 또는 치료용 조성물
CN112662644B (zh) * 2021-01-19 2022-04-22 华南理工大学 一种甘油磷酸二酯磷酸二酯酶突变体及其应用
CN113652440B (zh) * 2021-08-05 2023-04-21 昆明理工大学 3-酮脂酰辅酶a硫解酶基因rkacaa1-2及其应用
WO2023043945A2 (en) 2021-09-17 2023-03-23 Checkerspot, Inc. High oleic oil compositions and uses thereof
CN113621565B (zh) * 2021-10-08 2023-04-28 渤海水产股份有限公司 多肽在调控成纤维细胞体外培养中的应用
WO2023102069A1 (en) 2021-12-01 2023-06-08 Checkerspot, Inc. Polyols, polyurethane dispersions, and uses thereof
WO2023105498A1 (en) * 2021-12-10 2023-06-15 Phycoil Biotechnology International, Inc. Heterotrophic production of essential long-chain polyunsaturated lipids (lcpufa) in auxenochlorella protothecoides
CN114410674B (zh) * 2022-01-30 2024-02-23 深圳大学 一种提高衣藻半萜含量的转基因系统及其应用
CN114854596B (zh) * 2022-05-26 2023-04-14 珠海元育生物科技有限公司 高产蛋白质的原始小球藻突变株及其应用

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5900370A (en) 1985-07-01 1999-05-04 Bio-Technical Resources Process for the production of ascorbic acid with prototheca
US6395965B1 (en) 1997-03-21 2002-05-28 Restoragen, Inc. Plant containing a gene construct comprising a chlorella virus promoter and a lac operator
AU2002311787A1 (en) 2001-03-28 2002-10-15 Zycos Inc. Translational profiling
US7135290B2 (en) 2003-04-12 2006-11-14 Solazyme, Inc. Methods and compositions for evolving hydrogenase genes
US8258109B2 (en) 2005-10-20 2012-09-04 Isis Pharmaceuticals, Inc. Compositions and methods for modulation of LMNA expression
EP2044207A1 (en) 2006-07-11 2009-04-08 Basf Se Protein targeting to lipid bodies
AU2008259834B2 (en) 2007-06-01 2013-08-01 Corbion Biotech, Inc. Production of oil in microorganisms
US8455239B2 (en) 2007-12-23 2013-06-04 Gevo, Inc. Yeast organism producing isobutanol at a high yield
CN101230364A (zh) 2008-02-25 2008-07-30 清华大学 一种利用异养小球藻高密度发酵生产生物柴油的方法
MX2010011065A (es) 2008-04-09 2010-12-06 Solazyme Inc Modificacion química directa de biomasa microbiana y aceites microbianos.
US8435790B2 (en) 2008-07-25 2013-05-07 The Regents Of The University Of California Methods of modulating lipid concentrations in eukaryotic cells
CN104770424A (zh) 2008-10-14 2015-07-15 索拉兹米公司 微藻生物质的食品组合物
ES2583639T3 (es) 2008-11-28 2016-09-21 Terravia Holdings, Inc. Producción de aceites específicos en microorganismos heterótrofos
AU2010236412B2 (en) 2009-04-14 2015-10-01 Corbion Biotech, Inc. Methods of microbial oil extraction and separation
US8679782B2 (en) 2009-06-15 2014-03-25 Massachusetts Institute Of Technology Production of triacylglycerides, fatty acids, and their derivatives
WO2011008565A1 (en) 2009-06-29 2011-01-20 Synthetic Genomics, Inc. Acyl-acp thioesterase genes and uses therefor
JP5868962B2 (ja) * 2010-05-28 2016-02-24 ソラザイム, インコーポレイテッドSolazyme Inc 組み換え従属栄養性微生物から産生された用途に応じた油
KR101964965B1 (ko) 2011-02-02 2019-04-03 테라비아 홀딩스 인코포레이티드 재조합 유지성 미생물로부터 생산된 맞춤 오일
BR112013023613A2 (pt) 2011-03-17 2017-09-19 Solazyme Inc método de gaseificação, método de conversão da biomassa de um micróbio oleaginoso, composição torrificada combustível, e, combustível combinado
WO2013056212A2 (en) 2011-10-14 2013-04-18 Phycal Llc Chlorophyte genes for the optimization of productivity
BR112014012917A2 (pt) 2011-11-28 2017-06-13 Solazyme Inc ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, e, óleo microbiano
BR112014025719A8 (pt) 2012-04-18 2017-10-03 Solazyme Inc Óleos customizados
US9181568B2 (en) 2012-04-23 2015-11-10 Exxonmobil Research And Engineering Company Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases
US10612034B2 (en) 2012-06-01 2020-04-07 Exxonmobil Research And Engineering Company Promoters and terminators for use in eukaryotic cells
WO2014089514A1 (en) 2012-12-07 2014-06-12 Solazyme, Inc. Genetically engineered microbial strains including chlorella protothecoides lipid pathway genes

Also Published As

Publication number Publication date
EP2929032A1 (en) 2015-10-14
US20140178950A1 (en) 2014-06-26
EP2929032A4 (en) 2016-04-06
WO2014089514A1 (en) 2014-06-12
CN104968788A (zh) 2015-10-07
US9518277B2 (en) 2016-12-13

Similar Documents

Publication Publication Date Title
BR112015013193A2 (pt) ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, óleo microbiano, e, método para obter óleo microbiano.
BR112014012917A2 (pt) ácido nucleico recombinante, cassete de expressão, célula, método para obter óleo microbiano, e, óleo microbiano
BR112013030520A2 (pt) método, sistema, e, composição.
BR112013026261A2 (pt) método, e um ou mais meios de armazenamento legíveis por computador
BR112012031695A2 (pt) método, aparelho, meio de armazenamento legível por computador e produto de programa de computador
BR112014032116A2 (pt) método.
BR112015002638A2 (pt) método para marcar informações, e aparelho
BR112013015581A2 (pt) método, e. sistema
BR112013004072A2 (pt) aparelho, método e meio de armazenamento legível por computador
BR112013011732A2 (pt) método e sistema para realizar medidas ambientais, e, meio de armazenagem legível por máquina
BR112014001258A2 (pt) método de tipagem de dna, e, conjuntos de iniciadores para tipagem de dna
BR112014001992A2 (pt) método, sistema, e um ou mais meios de armazenamento legíveis por computador
BRPI0924664A2 (pt) eletrodo ,e ,método para construir um eletrodo.
AP2014007996A0 (en) Information sharing method, device and storage medium
BR112014026864A2 (pt) sistema, e método
AP2014007644A0 (en) Microblog display control method, microblog clientand computer storage medium
BR112014032104A2 (pt) método para identificar interações proteína-droga, e, produto de computador.
BR112013004193A2 (pt) método, aparelho, meio de armazenamento legível por computador e produto de programa de computador
BR112013005876A2 (pt) método, aparelho, meio de armazenamento legível por computador e produto de programa de computador
BR112012031266A2 (pt) método, aparelho, meio legível por computador para armazenar as instruções de programa e sistema.
BR112015008111A2 (pt) célula de levedura geneticamente modificada, e , método para a produção de 3hp.
BR112014007013A2 (pt) sistema, método e programa de computador produzido para gerenciamento de centro de contato
BR112014032838A2 (pt) método , e aparelho.
BR112014027958A2 (pt) método implementado de computador, meio de armazenamento legível por computador não transitório, e, sistema.
BR112013030873A2 (pt) sistema e dispositivo de provisão de informação, método de controle para um sistema de provisão de informação, programa, e, meio de armazenamento.

Legal Events

Date Code Title Description
B08F Application fees: dismissal - article 86 of industrial property law
B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)
B25E Requested change of name of applicant rejected

Owner name: SOLAZYME, INC. (US)