BR112015007577A2 - métodos para cultivar sebócitos primários e identificar compostos que regulam a lipogênese e população isolada de sebócitos cultivados - Google Patents

métodos para cultivar sebócitos primários e identificar compostos que regulam a lipogênese e população isolada de sebócitos cultivados

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Publication number
BR112015007577A2
BR112015007577A2 BR112015007577A BR112015007577A BR112015007577A2 BR 112015007577 A2 BR112015007577 A2 BR 112015007577A2 BR 112015007577 A BR112015007577 A BR 112015007577A BR 112015007577 A BR112015007577 A BR 112015007577A BR 112015007577 A2 BR112015007577 A2 BR 112015007577A2
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BR
Brazil
Prior art keywords
sebocytes
methods
cultured
identifying compounds
isolated population
Prior art date
Application number
BR112015007577A
Other languages
English (en)
Inventor
J Mcnaim Adrian
GAUSCH Geraldine
Original Assignee
Cincinnati Childrens Hospital Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cincinnati Childrens Hospital Medical Center filed Critical Cincinnati Childrens Hospital Medical Center
Publication of BR112015007577A2 publication Critical patent/BR112015007577A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0633—Cells of secretory glands, e.g. parotid gland, salivary glands, sweat glands, lacrymal glands
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C12N2533/12—Glass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

“métodos para cultivar sebócitos primários e identificar compostos que regulam a lipogênese e população isolada de sebócitos cultivados” resumo trata-se de métodos para cultivar sebócitos, populações isoladas de sebócitos e métodos para usar as sebócitos cultivadas para triar compostos que inibam ou ativem a lipogênese. 2/1 1/1
BR112015007577A 2012-10-05 2013-10-04 métodos para cultivar sebócitos primários e identificar compostos que regulam a lipogênese e população isolada de sebócitos cultivados BR112015007577A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261710359P 2012-10-05 2012-10-05
US201361765861P 2013-02-18 2013-02-18
PCT/US2013/063367 WO2014055815A1 (en) 2012-10-05 2013-10-04 Sebocyte cell culturing and methods of use

Publications (1)

Publication Number Publication Date
BR112015007577A2 true BR112015007577A2 (pt) 2017-12-12

Family

ID=50435450

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015007577A BR112015007577A2 (pt) 2012-10-05 2013-10-04 métodos para cultivar sebócitos primários e identificar compostos que regulam a lipogênese e população isolada de sebócitos cultivados

Country Status (7)

Country Link
US (1) US20150268254A1 (pt)
EP (1) EP2904095A4 (pt)
JP (1) JP2015530122A (pt)
CN (1) CN105247040A (pt)
BR (1) BR112015007577A2 (pt)
SG (1) SG11201502497RA (pt)
WO (1) WO2014055815A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10478526B2 (en) * 2013-05-03 2019-11-19 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Skin substitutes and methods for hair follicle neogenesis
WO2017138078A1 (ja) * 2016-02-08 2017-08-17 花王株式会社 成熟皮脂腺細胞の製造方法
FR3061205A1 (fr) * 2016-12-22 2018-06-29 L'oreal Modele d'epithelium reconstruit sebocytaire differencie a partir de sebocytes humains primaires
US20200003756A1 (en) * 2017-04-20 2020-01-02 Mandom Corporation Method for observing sebaceous gland
JP6692593B1 (ja) * 2018-10-17 2020-05-13 株式会社マンダム 皮脂腺の観察方法およびその利用
CN109852576A (zh) * 2019-01-07 2019-06-07 施歌 一种永生化皮脂腺细胞株的构建方法
CN111088217B (zh) * 2019-12-20 2023-03-03 广东博溪生物科技有限公司 细胞培养基、细胞培养试剂盒及细胞培养方法
CN112608947B (zh) * 2020-12-28 2023-06-16 上海市皮肤病医院 一种永生化人皮脂腺细胞系的构建方法及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5762947A (en) * 1997-05-09 1998-06-09 Elizabeth Arden Co., Division Of Conopco, Inc. Cosmetic skin conditioning compositions containing a salicyloxy α-carboxy acid
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞

Also Published As

Publication number Publication date
EP2904095A4 (en) 2016-03-30
CN105247040A (zh) 2016-01-13
EP2904095A1 (en) 2015-08-12
JP2015530122A (ja) 2015-10-15
US20150268254A1 (en) 2015-09-24
WO2014055815A1 (en) 2014-04-10
SG11201502497RA (en) 2015-04-29

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B350 Update of information on the portal [chapter 15.35 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]