BR112014031509B8 - Método para a determinação simultânea in vitro em tempo real do curso da atividade da enzima proteolítica e da resistência do coágulo de fibrina, reômetro rotativo e uso de um reômetro rotativo - Google Patents

Método para a determinação simultânea in vitro em tempo real do curso da atividade da enzima proteolítica e da resistência do coágulo de fibrina, reômetro rotativo e uso de um reômetro rotativo

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Publication number
BR112014031509B8
BR112014031509B8 BR112014031509A BR112014031509A BR112014031509B8 BR 112014031509 B8 BR112014031509 B8 BR 112014031509B8 BR 112014031509 A BR112014031509 A BR 112014031509A BR 112014031509 A BR112014031509 A BR 112014031509A BR 112014031509 B8 BR112014031509 B8 BR 112014031509B8
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BR
Brazil
Prior art keywords
rotary rheometer
proteolytic enzyme
simultaneous
course
time determination
Prior art date
Application number
BR112014031509A
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English (en)
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BR112014031509A2 (pt
BR112014031509B1 (pt
Inventor
Bas De Laat H
Coenraad Hemker Hendrik
Hilde Kelchtermans
Leonie Pelkmans
Original Assignee
Synapse Bv
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Publication date
Application filed by Synapse Bv filed Critical Synapse Bv
Publication of BR112014031509A2 publication Critical patent/BR112014031509A2/pt
Publication of BR112014031509B1 publication Critical patent/BR112014031509B1/pt
Publication of BR112014031509B8 publication Critical patent/BR112014031509B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
    • G01N11/10—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material
    • G01N11/14—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by moving a body within the material by using rotary bodies, e.g. vane
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4905—Determining clotting time of blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Ecology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

método para a determinação simultânea in vitro em tempo real do curso da atividade da enzima proteolítica e da resistência do coágulo de fibrina, reômetro rotativo e uso de um reômetro rotativo. a presente invenção se refere à medição simultânea da geração da enzima proteolítica e da resistência do coágulo no plasma ou sangue total ou de qualquer amostra biológica adequada derivada do sangue. o método de medição abrange a utilização de um substrato detectável, que compreende uma porção que pode ser liberada mediante reação com a enzima proteolítica alvo, e os meios para medição de um aumento na viscosidade da resistência do coágulo.
BR112014031509A 2012-06-21 2013-06-20 Método para a determinação simultânea in vitro em tempo real do curso da atividade da enzima proteolítica e da resistência do coágulo de fibrina, reômetro rotativo e uso de um reômetro rotativo BR112014031509B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12173053.5 2012-06-21
EP12173053.5A EP2677037B1 (en) 2012-06-21 2012-06-21 Simultaneous measurement of thrombin generation and clot strength in plasma and whole blood
PCT/EP2013/062949 WO2013190071A2 (en) 2012-06-21 2013-06-20 Simultaneous measurement of thrombin generation and clot strength in plasma and whole blood

Publications (3)

Publication Number Publication Date
BR112014031509A2 BR112014031509A2 (pt) 2017-08-01
BR112014031509B1 BR112014031509B1 (pt) 2021-11-23
BR112014031509B8 true BR112014031509B8 (pt) 2022-05-24

Family

ID=48652124

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014031509A BR112014031509B8 (pt) 2012-06-21 2013-06-20 Método para a determinação simultânea in vitro em tempo real do curso da atividade da enzima proteolítica e da resistência do coágulo de fibrina, reômetro rotativo e uso de um reômetro rotativo

Country Status (5)

Country Link
US (1) US9428790B2 (pt)
EP (1) EP2677037B1 (pt)
JP (1) JP6300793B2 (pt)
BR (1) BR112014031509B8 (pt)
WO (1) WO2013190071A2 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009308841B2 (en) 2008-10-29 2014-07-17 T2 Biosystems, Inc. NMR detection of coagulation time
AU2012281017B2 (en) 2011-07-13 2017-07-20 T2 Biosystems, Inc. NMR methods for monitoring blood clot formation
WO2013043858A1 (en) 2011-09-21 2013-03-28 T2 Biosystems, Inc. Nmr methods for endotoxin analysis
US9739733B2 (en) 2012-12-07 2017-08-22 T2 Biosystems, Inc. Methods for monitoring tight clot formation
US10823743B1 (en) 2013-10-28 2020-11-03 Ifirst Medical Technologies, Inc. Methods of measuring coagulation of a biological sample
US20170045494A1 (en) * 2014-04-28 2017-02-16 T2 Biosystems, Inc. Systems and methods for identifying coagulopathies
FR3024237B1 (fr) * 2014-07-25 2016-08-05 Stago Diagnostica Methode de determination du profil de structure d'un caillot de fibrine, refletant sa stabilite, pour predire le risque de saignement, de thrombose ou de re-thrombose
US10295554B2 (en) * 2015-06-29 2019-05-21 C A Casyso Gmbh Blood testing system and method
JP7101351B2 (ja) 2016-04-28 2022-07-15 シスメックス株式会社 血液検体の分析方法、血液検体分析用試薬及び試薬キット、並びに血液検体分析装置
EP3363912B1 (en) * 2017-02-17 2020-10-28 Synapse B.V. Self-calibrated assay for determining proteolytic enzyme generation, dedicated means and uses thereof
EP4251998A1 (en) * 2020-11-30 2023-10-04 Viscop S.r.l. Method of acquisition and analysis of coagulation haemostatic parameters of a blood sample
JP2022122022A (ja) * 2021-02-09 2022-08-22 キヤノンメディカルシステムズ株式会社 血液特性評価装置、血液特性評価方法及びプログラム
WO2022188189A1 (zh) * 2021-03-12 2022-09-15 重庆南方数控设备股份有限公司 一种基于磁珠的血栓弹力图检测装置及方法
CN113340772B (zh) * 2021-06-11 2022-06-17 季华实验室 一种监测dna水凝胶凝胶化转变过程的方法
CN116593356B (zh) * 2023-06-05 2023-11-17 南京工业大学 一种利用磁纳米毛刷搅拌检测微量溶液粘度的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8902406A (nl) 1989-09-27 1991-04-16 Hendrik Coenraad Hemker Prof D Werkwijze voor het bepalen van de endogene trombinepotentiaal van plasma, en bloed alsmede een bij deze werkwijze te gebruiken kit.
ES2247820T3 (es) * 1998-05-25 2006-03-01 Jennissen, Herbert P. Analizador de cizallamiento de circulacion para moleculas biologicas activas en capas de liquidos en superficies, procedimiento para los analisis de un liquido y procedimiento para determinar un espesor de una capa de liquido ultrafina.
AT409422B (de) 1999-11-29 2002-08-26 Anton Paar Gmbh Rotationsrheometer
JP4058512B2 (ja) * 2001-01-18 2008-03-12 独立行政法人産業技術総合研究所 コーンプレート型血液等の適合性評価装置
AU2003232720B9 (en) 2002-05-01 2009-12-03 Synapse B.V. Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media
EP1717588A1 (en) * 2005-04-29 2006-11-02 Synapse B.V. Measuring thrombin activity in whole blood
GB0703004D0 (en) 2007-02-15 2007-03-28 Uws Ventures Ltd Apparatus and method for measuring rheological properties of blood
US20100273206A1 (en) 2007-12-21 2010-10-28 Manfred Rauh Diagnostic in vitro method for assessing von willebrand disease and increased bleeding risk associated with von willebrand disease and acquired or congenital disorders of platelet function
EP2088435B1 (en) 2008-02-07 2011-07-13 Synapse B.V. Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate
US8293493B2 (en) 2010-01-27 2012-10-23 Adventist Health System/Sunbelt, Inc. Thrombin generation determination method
US11172888B2 (en) * 2012-12-19 2021-11-16 The General Hospital Corporation Optical blood-coagulation sensor

Also Published As

Publication number Publication date
EP2677037B1 (en) 2016-04-13
US9428790B2 (en) 2016-08-30
BR112014031509A2 (pt) 2017-08-01
JP2015521738A (ja) 2015-07-30
JP6300793B2 (ja) 2018-03-28
BR112014031509B1 (pt) 2021-11-23
WO2013190071A2 (en) 2013-12-27
WO2013190071A3 (en) 2014-03-20
US20150118691A1 (en) 2015-04-30
EP2677037A1 (en) 2013-12-25

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