BR112013029824A2 - análise e classificação de células móveis - Google Patents

análise e classificação de células móveis

Info

Publication number
BR112013029824A2
BR112013029824A2 BR112013029824A BR112013029824A BR112013029824A2 BR 112013029824 A2 BR112013029824 A2 BR 112013029824A2 BR 112013029824 A BR112013029824 A BR 112013029824A BR 112013029824 A BR112013029824 A BR 112013029824A BR 112013029824 A2 BR112013029824 A2 BR 112013029824A2
Authority
BR
Brazil
Prior art keywords
population
cells
microfluidic channel
mobile
average motility
Prior art date
Application number
BR112013029824A
Other languages
English (en)
Inventor
Emre Kayaalp
Hooman Safaee
Savas Tasoglu
Utkan Demirci
Xiaohui Zhang
Original Assignee
Brigham & Womens Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47217995&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112013029824(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Brigham & Womens Hospital filed Critical Brigham & Womens Hospital
Publication of BR112013029824A2 publication Critical patent/BR112013029824A2/pt

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0694—Creating chemical gradients in a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0003—Determining electric mobility, velocity profile, average speed or velocity of a plurality of particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0042—Investigating dispersion of solids
    • G01N2015/0053—Investigating dispersion of solids in liquids, e.g. trouble
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Fluid Mechanics (AREA)
  • Gynecology & Obstetrics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

abstract of the disclosure analysis and sorting of motile cells a method for sorting motile cells includes introducing an initial population of motile cells into an inlet port of a microfluidic channel, the initial population of motile cells having a first average motility; incubating the population of motile cells in the microfluidic channel; and collecting a sorted population of motile cells at an outlet port of the microfluidic channel. the sorted population of motile cells has a second average motility higher than the first average motility. _______________________________________________________________________ tradução do resumo resumo patente de invenção: "análise e classificação de células móveis". a presente invenção se refere a um método para classificar células móveis que inclui introduzir uma população inicial de células móveis em uma porta de entrada de um canal microfluídico, a população inicial de células móveis tendo uma primeira motilidade média; incubar a população de células móveis no canal microfluídico; e coletar a população classificada de células móveis em uma porta de saída do canal microfluídico. a população classificada de células móveis tem uma segunda motilidade média mais alta do que a primeira motilidade média.
BR112013029824A 2011-05-20 2012-05-18 análise e classificação de células móveis BR112013029824A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161488300P 2011-05-20 2011-05-20
PCT/US2012/038680 WO2012162181A2 (en) 2011-05-20 2012-05-18 Analysis and sorting of motile cells

Publications (1)

Publication Number Publication Date
BR112013029824A2 true BR112013029824A2 (pt) 2016-09-06

Family

ID=47217995

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013029824A BR112013029824A2 (pt) 2011-05-20 2012-05-18 análise e classificação de células móveis

Country Status (10)

Country Link
US (3) US20140248656A1 (pt)
EP (1) EP2710139B2 (pt)
JP (2) JP6196614B2 (pt)
CN (2) CN108889345B (pt)
AU (1) AU2012258999B2 (pt)
BR (1) BR112013029824A2 (pt)
CA (1) CA2836774C (pt)
ES (1) ES2702793T5 (pt)
TR (1) TR201819543T4 (pt)
WO (1) WO2012162181A2 (pt)

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CN102242055B (zh) * 2011-06-03 2013-08-14 博奥生物有限公司 一种精子活力评价及筛选的方法及其专用微流控芯片装置
US9222874B2 (en) * 2012-06-27 2015-12-29 The United States Of America As Represented By The Secretary Of The Army Systems and methods for individually trapping particles from air and measuring the optical spectra or other properties of individual trapped particles
FR3002634B1 (fr) * 2013-02-28 2015-04-10 Commissariat Energie Atomique Procede d'observation d'au moins un objet, tel qu'une entite biologique, et systeme d'imagerie associe
BR112015023155B1 (pt) * 2013-03-14 2022-09-20 Inguran, Llc Dispositivo e métodos de triagem de elevado rendimento de espermatozóides
US9757726B2 (en) 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
US10662408B2 (en) 2013-03-14 2020-05-26 Inguran, Llc Methods for high throughput sperm sorting
US10371622B2 (en) 2013-03-14 2019-08-06 Inguran, Llc Device for high throughput sperm sorting
EP2991765B3 (en) * 2013-05-03 2023-06-28 Motilitycount Aps Device for analysis of cellular motility
EP3071704B1 (en) 2013-11-20 2022-03-30 Brigham and Women's Hospital, Inc. System and method for sperm sorting
US9574991B2 (en) * 2014-10-14 2017-02-21 Omnivision Technologies, Inc. High-throughput fluorescence imaging system and device with sample heating capability, and associated methods
CN105403499A (zh) * 2015-12-31 2016-03-16 中国人民解放军第三军医大学第一附属医院 用于光学检测的单细胞流道装置
DK3239689T3 (da) * 2016-04-26 2022-01-10 Atten2 Advanced Monitoring Tech S L Fluidovervågningssystem
US20200209221A1 (en) * 2016-12-08 2020-07-02 Sigtuple Technologies Private Limited A method and system for evaluating quality of semen sample
AU2018216627B2 (en) 2017-01-31 2023-07-13 Genea Ip Holdings Pty Limited Method and system for processing a biological sample
JP6871116B2 (ja) * 2017-09-15 2021-05-12 株式会社東芝 セルソータ
WO2019064040A1 (en) * 2017-09-29 2019-04-04 Nanovare Sas DEVICES AND METHODS FOR ANALYZING MALE INFERTILITY
US11491485B2 (en) 2018-04-09 2022-11-08 Cornell University Rheotaxis-based separation of motile sperm and bacteria using a microfluidic corral system
CN109932290B (zh) * 2019-01-16 2020-10-20 中国科学院水生生物研究所 一种基于流式影像运动目标追踪的颗粒计数方法
CN109828423A (zh) * 2019-02-28 2019-05-31 西安理工大学 应用于无透镜成像装置的光源系统
JP6661039B1 (ja) * 2019-03-28 2020-03-11 国立大学法人 東京大学 運動性細胞の選択装置
WO2021124111A1 (en) * 2019-12-16 2021-06-24 King Abdullah University Of Science And Technology Microfluidic device for highly motile sperm selection
CN111339904B (zh) * 2020-02-21 2023-11-03 腾讯科技（深圳）有限公司 动物精子图像识别方法和装置
CN111323361B (zh) * 2020-03-17 2022-05-10 南通大学 一种快速分离精子头、精子尾和正常有活力精子的方法
EP4005672A1 (en) * 2020-11-25 2022-06-01 Pera Labs Medikal Arastirma ve Gelistirme Limited Sirketi A device for separating and analyzing seminal sample, system and method
WO2022265716A1 (en) * 2021-06-14 2022-12-22 Becton, Dickinson And Company Clamps for applying an immobilizing force to a photodetector, and systems and methods for using the same
FR3136479A1 (fr) 2022-06-13 2023-12-15 Béez Biotech Dispositif d’isolement de cellules de sperme et procédé de sélection de cellules de sperme de haute qualité
WO2024026018A2 (en) * 2022-07-27 2024-02-01 The Trustees Of The University Of Columbia In The City Of New York Systems and methods for identifying and recovering rare biological cells from a sample

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IT1307787B1 (it) * 1999-07-26 2001-11-19 Univ Firenze Processo per incrementare la motilita' degli spermatozoi e spermatozoia motilita' superiore cosi' ottenuti.
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Also Published As

Publication number Publication date
EP2710139B1 (en) 2018-09-19
TR201819543T4 (tr) 2019-01-21
JP2014518627A (ja) 2014-08-07
JP2017169570A (ja) 2017-09-28
CN108889345B (zh) 2020-10-09
WO2012162181A3 (en) 2013-01-17
WO2012162181A2 (en) 2012-11-29
US20220073872A1 (en) 2022-03-10
CA2836774A1 (en) 2012-11-29
US20140248656A1 (en) 2014-09-04
US20190316084A1 (en) 2019-10-17
EP2710139A4 (en) 2014-10-29
AU2012258999B2 (en) 2016-11-17
CA2836774C (en) 2023-07-04
CN108889345A (zh) 2018-11-27
CN103781916A (zh) 2014-05-07
JP6196614B2 (ja) 2017-09-13
EP2710139A2 (en) 2014-03-26
ES2702793T5 (es) 2022-07-15
ES2702793T3 (es) 2019-03-05
EP2710139B2 (en) 2022-04-13

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