BR112013028625A2 - lise seletiva de células através de tensoativos iônicos - Google Patents

lise seletiva de células através de tensoativos iônicos

Info

Publication number
BR112013028625A2
BR112013028625A2 BR112013028625A BR112013028625A BR112013028625A2 BR 112013028625 A2 BR112013028625 A2 BR 112013028625A2 BR 112013028625 A BR112013028625 A BR 112013028625A BR 112013028625 A BR112013028625 A BR 112013028625A BR 112013028625 A2 BR112013028625 A2 BR 112013028625A2
Authority
BR
Brazil
Prior art keywords
cell lysis
ionic surfactants
selective cell
selective
sample
Prior art date
Application number
BR112013028625A
Other languages
English (en)
Inventor
Bart Van Meerbergen
Irene Dobbelaer
Paul Van De Wiel
Roel Penterman
Sieglinde Neerken
Original Assignee
Biocartis Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocartis Sa filed Critical Biocartis Sa
Publication of BR112013028625A2 publication Critical patent/BR112013028625A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

lise seletiva de células através de tensoativos iônicos a presente invenção refere-se a métodos, kits de componentes e dispositivos para a lise de células eucarióticas em uma amostra que compreende micro-organismos tais como bactérias ou fungos unicelulares. a lise seletiva é obtida através da incubação da amostra em um tensoativo iônico sob condições alcalinas.
BR112013028625A 2011-06-06 2012-04-30 lise seletiva de células através de tensoativos iônicos BR112013028625A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11305692 2011-06-06
PCT/EP2012/057897 WO2012168003A1 (en) 2011-06-06 2012-04-30 Selective lysis of cells by ionic surfactants

Publications (1)

Publication Number Publication Date
BR112013028625A2 true BR112013028625A2 (pt) 2017-01-24

Family

ID=46025697

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013028625A BR112013028625A2 (pt) 2011-06-06 2012-04-30 lise seletiva de células através de tensoativos iônicos

Country Status (11)

Country Link
US (1) US9863006B2 (pt)
EP (1) EP2718713B1 (pt)
JP (2) JP6153516B2 (pt)
KR (1) KR20140023326A (pt)
CN (1) CN103518132B (pt)
AU (1) AU2012266754B2 (pt)
BR (1) BR112013028625A2 (pt)
CA (1) CA2831969A1 (pt)
RU (1) RU2013153507A (pt)
WO (1) WO2012168003A1 (pt)
ZA (1) ZA201308935B (pt)

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Also Published As

Publication number Publication date
JP2014516554A (ja) 2014-07-17
CA2831969A1 (en) 2012-12-30
US20140087361A1 (en) 2014-03-27
EP2718713B1 (en) 2019-07-17
ZA201308935B (en) 2015-05-27
CN103518132A (zh) 2014-01-15
JP2017093464A (ja) 2017-06-01
EP2718713A1 (en) 2014-04-16
AU2012266754A1 (en) 2013-10-24
RU2013153507A (ru) 2015-07-20
CN103518132B (zh) 2015-11-25
JP6153516B2 (ja) 2017-06-28
WO2012168003A1 (en) 2012-12-13
AU2012266754B2 (en) 2016-04-21
KR20140023326A (ko) 2014-02-26
US9863006B2 (en) 2018-01-09

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Owner name: BIOCARTIS SA (CH)