BR112013014912B1 - Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol - Google Patents

Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol Download PDF

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Publication number
BR112013014912B1
BR112013014912B1 BR112013014912-4A BR112013014912A BR112013014912B1 BR 112013014912 B1 BR112013014912 B1 BR 112013014912B1 BR 112013014912 A BR112013014912 A BR 112013014912A BR 112013014912 B1 BR112013014912 B1 BR 112013014912B1
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BR
Brazil
Prior art keywords
seq
zymomonas
ethanol
xylose
production
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BR112013014912-4A
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English (en)
Portuguese (pt)
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BR112013014912A2 (pt
Inventor
Perry G. Caimi
William D. Hitz
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E.I. Du Pont De Nemours And Company
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Publication of BR112013014912A2 publication Critical patent/BR112013014912A2/pt
Publication of BR112013014912B1 publication Critical patent/BR112013014912B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112013014912-4A 2010-12-17 2011-12-13 Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol BR112013014912B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061424077P 2010-12-17 2010-12-17
US61/424,077 2010-12-17
PCT/US2011/064580 WO2012082711A1 (en) 2010-12-17 2011-12-13 Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium

Publications (2)

Publication Number Publication Date
BR112013014912A2 BR112013014912A2 (pt) 2018-05-15
BR112013014912B1 true BR112013014912B1 (pt) 2021-06-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013014912-4A BR112013014912B1 (pt) 2010-12-17 2011-12-13 Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol

Country Status (8)

Country Link
US (2) US8476048B2 (enExample)
EP (1) EP2652121A1 (enExample)
JP (1) JP2013545491A (enExample)
CN (1) CN103261400A (enExample)
AU (1) AU2011344023A1 (enExample)
BR (1) BR112013014912B1 (enExample)
CA (1) CA2821135A1 (enExample)
WO (1) WO2012082711A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2012003091A (es) 2009-09-23 2012-04-30 Danisco Us Inc Enzimas de glicosil hidrolasa novedosas y usos de las mismas.
AU2010333801B2 (en) 2009-12-23 2015-12-17 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
JP6290626B2 (ja) * 2011-03-17 2018-03-14 ダニスコ・ユーエス・インク 糖化プロセスにおける粘度を低下させる方法
BR112014031024A2 (pt) 2012-06-11 2017-06-27 Codexis Inc xilanases e xilosidases fúngicas.
US8846357B2 (en) 2012-12-20 2014-09-30 E I Du Pont De Nemours And Company Stabilized chlorine dioxide for contamination control in Zymomonas fermentation
US20140273105A1 (en) 2013-03-12 2014-09-18 E I Du Pont De Nemours And Company Gradient pretreatment of lignocellulosic biomass
CN110564663B (zh) 2013-08-19 2023-03-10 辛格隆股份公司 微生物的受控生长
US9499451B2 (en) 2013-10-10 2016-11-22 E I Du Pont De Nemours And Company Soil conditioner compositions containing lignocellulosic biomass fermentation process syrup
US9873846B2 (en) 2013-10-10 2018-01-23 E I Du Pont De Nemours And Company Fuel compositions containing lignocellulosic biomass fermentation process syrup
US9725363B2 (en) 2013-10-10 2017-08-08 E I Du Pont De Nemours And Company Lignocellulosic biomass fermentation process co-product fuel for cement kiln
CN103810391B (zh) * 2014-02-21 2017-05-17 清华大学 运动发酵单胞菌生产乙醇中振荡过程的调控方法
US9371546B2 (en) 2014-06-24 2016-06-21 E I Du Pont De Nemours And Company Enhancing D-xylose and L-arabinose utilization in Zymomonas cells
WO2016044606A1 (en) 2014-09-17 2016-03-24 Danisco Us Inc Simultaneous saccharification and fermentation process in the presence of benzoate
CN111757890A (zh) 2017-12-19 2020-10-09 辛格隆股份公司 发酵工艺

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
AU2388295A (en) 1994-04-15 1995-11-10 Midwest Research Institute Recombinant zymomonas for pentose fermentation
US5712133A (en) 1994-04-15 1998-01-27 Midwest Research Institute Pentose fermentation by recombinant zymomonas
US5514583A (en) 1994-04-15 1996-05-07 Midwest Research Institute Recombinant zymomonas for pentose fermentation
US5843760A (en) 1994-04-15 1998-12-01 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
DE19856136C2 (de) 1998-12-04 2002-10-24 Pasteur Institut Verfahren und Vorrichtung zur Selektion beschleunigter Proliferation lebender Zellen in Suspension
US7223575B2 (en) 2000-05-01 2007-05-29 Midwest Research Institute Zymomonas pentose-sugar fermenting strains and uses thereof
US6566107B1 (en) 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
US20040231060A1 (en) 2003-03-07 2004-11-25 Athenix Corporation Methods to enhance the activity of lignocellulose-degrading enzymes
JP5804666B2 (ja) 2005-04-12 2015-11-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ バイオマスの処理および利用における別の供給流れの集中
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
US7741084B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas
US7803623B2 (en) 2007-10-30 2010-09-28 E.I. Du Pont De Nemours And Company Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate
DE602008006446D1 (de) 2007-10-30 2011-06-01 Du Pont Verfahren zur herstellung von etahnol in einem xylose enthaltenden medium unter verwendung eines rekombinanten zymomonas stammes mit reduzierter hima expression
EP2271755B1 (en) 2008-03-27 2017-03-22 E. I. du Pont de Nemours and Company High expression zymomonas promoters
US7998722B2 (en) 2008-03-27 2011-08-16 E. I. Du Pont De Nemours And Company Zymomonas with improved xylose utilization
US8329444B2 (en) 2008-04-23 2012-12-11 Georgia Tech Research Corporation Strains of zymomonas mobilis for fermentation of biomass
US8247208B2 (en) 2008-12-22 2012-08-21 Alliance For Sustainable Energy Llc Zymomonas with improved xylose utilization in stress conditions
US20110143408A1 (en) 2009-06-18 2011-06-16 E. I. Du Pont De Nemours And Company Zymomonas with improved arabinose utilization
CN101781634B (zh) * 2009-12-04 2011-09-28 天津大学 能利用木糖生产乙醇的重组运动发酵单胞菌及发酵方法
US8906235B2 (en) 2010-04-28 2014-12-09 E I Du Pont De Nemours And Company Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth
US8623623B2 (en) 2010-06-29 2014-01-07 E I Du Pont De Nemours And Company Xylose utilization in recombinant Zymomonas

Also Published As

Publication number Publication date
BR112013014912A2 (pt) 2018-05-15
US20120329114A1 (en) 2012-12-27
US8569458B2 (en) 2013-10-29
JP2013545491A (ja) 2013-12-26
EP2652121A1 (en) 2013-10-23
US8476048B2 (en) 2013-07-02
WO2012082711A1 (en) 2012-06-21
US20130177958A1 (en) 2013-07-11
CA2821135A1 (en) 2012-06-21
CN103261400A (zh) 2013-08-21
AU2011344023A1 (en) 2013-05-23

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B06A Patent application procedure suspended [chapter 6.1 patent gazette]
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B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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