BR112013014912B1 - Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol - Google Patents

Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol Download PDF

Info

Publication number
BR112013014912B1
BR112013014912B1 BR112013014912-4A BR112013014912A BR112013014912B1 BR 112013014912 B1 BR112013014912 B1 BR 112013014912B1 BR 112013014912 A BR112013014912 A BR 112013014912A BR 112013014912 B1 BR112013014912 B1 BR 112013014912B1
Authority
BR
Brazil
Prior art keywords
seq
zymomonas
ethanol
xylose
production
Prior art date
Application number
BR112013014912-4A
Other languages
English (en)
Portuguese (pt)
Other versions
BR112013014912A2 (pt
Inventor
Perry G. Caimi
William D. Hitz
Original Assignee
E.I. Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I. Du Pont De Nemours And Company filed Critical E.I. Du Pont De Nemours And Company
Publication of BR112013014912A2 publication Critical patent/BR112013014912A2/pt
Publication of BR112013014912B1 publication Critical patent/BR112013014912B1/pt

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112013014912-4A 2010-12-17 2011-12-13 Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol BR112013014912B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061424077P 2010-12-17 2010-12-17
US61/424,077 2010-12-17
PCT/US2011/064580 WO2012082711A1 (en) 2010-12-17 2011-12-13 Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium

Publications (2)

Publication Number Publication Date
BR112013014912A2 BR112013014912A2 (pt) 2018-05-15
BR112013014912B1 true BR112013014912B1 (pt) 2021-06-22

Family

ID=45444731

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013014912-4A BR112013014912B1 (pt) 2010-12-17 2011-12-13 Micro-organismo recombinante, polinucleotídeo, polipeptídeo, método para a produção de um zymomonas recombinante etanologênico e método para a produção de etanol

Country Status (8)

Country Link
US (2) US8476048B2 (enExample)
EP (1) EP2652121A1 (enExample)
JP (1) JP2013545491A (enExample)
CN (1) CN103261400A (enExample)
AU (1) AU2011344023A1 (enExample)
BR (1) BR112013014912B1 (enExample)
CA (1) CA2821135A1 (enExample)
WO (1) WO2012082711A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2480660B1 (en) 2009-09-23 2020-08-05 Danisco US Inc. Novel glycosyl hydrolase enzymes and uses thereof
UA114276C2 (uk) 2009-12-23 2017-05-25 Даніско Юес Інк. Спосіб одночасного оцукрювання і ферментації
CN103842515A (zh) * 2011-03-17 2014-06-04 丹尼斯科美国公司 降低糖化过程的粘度的方法
EP2859099B1 (en) * 2012-06-11 2019-05-08 Codexis, Inc. Fungal beta-xylosidase variants
US8846357B2 (en) 2012-12-20 2014-09-30 E I Du Pont De Nemours And Company Stabilized chlorine dioxide for contamination control in Zymomonas fermentation
US20140273105A1 (en) 2013-03-12 2014-09-18 E I Du Pont De Nemours And Company Gradient pretreatment of lignocellulosic biomass
BR122021015417B1 (pt) 2013-08-19 2022-11-08 Syngulon Sa Célula microbiana geneticamente engenheirada que compreende um ácido nucleico que codifica uma bacteriocina
US9725363B2 (en) 2013-10-10 2017-08-08 E I Du Pont De Nemours And Company Lignocellulosic biomass fermentation process co-product fuel for cement kiln
US20150101377A1 (en) 2013-10-10 2015-04-16 E I Du Pont De Nemours And Company Lignocellulosic biomass fermentation process co-product ash for land applications
US20150101751A1 (en) 2013-10-10 2015-04-16 E I Du Pont De Nemours And Company Lignocellulosic biomass fermentation process syrup binder and adhesive
CN103810391B (zh) * 2014-02-21 2017-05-17 清华大学 运动发酵单胞菌生产乙醇中振荡过程的调控方法
US9371546B2 (en) 2014-06-24 2016-06-21 E I Du Pont De Nemours And Company Enhancing D-xylose and L-arabinose utilization in Zymomonas cells
WO2016044606A1 (en) 2014-09-17 2016-03-24 Danisco Us Inc Simultaneous saccharification and fermentation process in the presence of benzoate
WO2019121983A1 (en) 2017-12-19 2019-06-27 Syngulon S.A. Fermentation process

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5712133A (en) 1994-04-15 1998-01-27 Midwest Research Institute Pentose fermentation by recombinant zymomonas
US5843760A (en) 1994-04-15 1998-12-01 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
US5514583A (en) 1994-04-15 1996-05-07 Midwest Research Institute Recombinant zymomonas for pentose fermentation
WO1995028476A1 (en) 1994-04-15 1995-10-26 Midwest Research Institute Recombinant zymomonas for pentose fermentation
DE19856136C2 (de) 1998-12-04 2002-10-24 Pasteur Institut Verfahren und Vorrichtung zur Selektion beschleunigter Proliferation lebender Zellen in Suspension
US7223575B2 (en) 2000-05-01 2007-05-29 Midwest Research Institute Zymomonas pentose-sugar fermenting strains and uses thereof
US6566107B1 (en) 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
US20040231060A1 (en) 2003-03-07 2004-11-25 Athenix Corporation Methods to enhance the activity of lignocellulose-degrading enzymes
US7998713B2 (en) 2005-04-12 2011-08-16 E. I. Du Pont De Nemours And Company Treatment of biomass to obtain ethanol
US7741119B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production
US7741084B2 (en) 2006-09-28 2010-06-22 E. I. Du Pont De Nemours And Company Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas
US7629156B2 (en) 2006-09-28 2009-12-08 E.I. Du Pont De Nemours And Company Ethanol production in fermentation of mixed sugars containing xylose
PL2209899T3 (pl) 2007-10-30 2012-09-28 Du Pont Zymomonas o ulepszonej produkcji etanolu w pożywce zawierającej stężone cukry i octan
BRPI0817152A8 (pt) 2007-10-30 2018-12-11 Du Pont processo para a produção de etanol
AU2009228323B2 (en) 2008-03-27 2014-01-30 Alliance For Sustainable Energy, Llc Zymomonas with improved xylose utilization
JP5444323B2 (ja) 2008-03-27 2014-03-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 高発現ザイモモナス（Ｚｙｍｏｍｏｎａｓ）プロモーター
BRPI0911467A2 (pt) 2008-04-23 2015-08-04 Georgia Tech Res Inst Processo para fabricar uma cepa de zymomonas mobilis, e, cepa mutante de zymomonas mobilis.
US8247208B2 (en) 2008-12-22 2012-08-21 Alliance For Sustainable Energy Llc Zymomonas with improved xylose utilization in stress conditions
US20110143408A1 (en) 2009-06-18 2011-06-16 E. I. Du Pont De Nemours And Company Zymomonas with improved arabinose utilization
CN101781634B (zh) * 2009-12-04 2011-09-28 天津大学 能利用木糖生产乙醇的重组运动发酵单胞菌及发酵方法
US8906235B2 (en) * 2010-04-28 2014-12-09 E I Du Pont De Nemours And Company Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth
US8623623B2 (en) 2010-06-29 2014-01-07 E I Du Pont De Nemours And Company Xylose utilization in recombinant Zymomonas

Also Published As

Publication number Publication date
AU2011344023A1 (en) 2013-05-23
CA2821135A1 (en) 2012-06-21
US8476048B2 (en) 2013-07-02
BR112013014912A2 (pt) 2018-05-15
CN103261400A (zh) 2013-08-21
JP2013545491A (ja) 2013-12-26
EP2652121A1 (en) 2013-10-23
US20130177958A1 (en) 2013-07-11
WO2012082711A1 (en) 2012-06-21
US20120329114A1 (en) 2012-12-27
US8569458B2 (en) 2013-10-29

Similar Documents

Publication Publication Date Title
US8569458B2 (en) Xylose utilizing Zymomonas mobilis with improved ethanol production in biomass hydrolysate medium
CA2803878C (en) Improved xylose utilization in recombinant zymomonas having additional xylose isomerase activity
EP2066779B1 (en) Improved ethanol production in fermentation of mixed sugars containing xylose in the presence of sugar alcohols
CN101970671B (zh) 在包含浓缩糖和乙酸盐的培养基中具有改进的乙醇产量的发酵单胞菌属
Thomas et al. Xylanase and cellulase systems of Clostridium sp.: an insight on molecular approaches for strain improvement
BRPI0918395B1 (pt) método para produzir uma linhagem de zymomonas que utiliza xilose
JP2011515067A (ja) ｈｉｍＡ発現の減少した組み換えザイモモナス株を使用する、キシロースを含む培地からエタノールを産生する方法
BRPI0715282A2 (pt) linhagem de zymomonas recombinante e processo de geraÇço de um linhagem de zymomonas
BRPI0813710B1 (pt) método de identificar um polipeptídeo heterólogo tendo atividade enzimática para converter piruvato, acetaldeído ou acetato em acetil-coa no citosol de uma célula de levedura, vetor para a expressão de polipeptídeos heterólogos em levedura, célula de levedura recombinante e método de produzir um produto de fermentação
US20110143408A1 (en) Zymomonas with improved arabinose utilization
EP2861748B1 (en) Use of hop acids for bacterial contamination control in fermentations using zymomonas mobilis
US9522203B2 (en) Stabilized chlorine dioxide for contamination control in zymomonas fermentation
BR112014015566B1 (pt) Célula hospedeira bacteriana recombinante e processo para produção de etanol
EP2861747B1 (en) Use of virginiamycin for bacterial contamination control in fermentations using zymomonas mobilis
Lulu et al. Draft Genome Sequence of Thermophilic Bacillus sp. TYF-LIM-B05 Directly Producing Ethanol from Various Carbon Sources Including Lignocellulose
US9371546B2 (en) Enhancing D-xylose and L-arabinose utilization in Zymomonas cells
Groom Jr Genetic tools to enable biofuel production in thermophilic cellulose-degrading bacteria
WO2012108740A2 (en) Mutant microorganism with enhanced sugar utilization and methods for preparing the same

Legal Events

Date Code Title Description
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/12/2011, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 14A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2859 DE 21-10-2025 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.