BR0307797A - Mutantes termoestáveis de enzimas da biossìntese de amido - Google Patents

Mutantes termoestáveis de enzimas da biossìntese de amido

Info

Publication number
BR0307797A
BR0307797A BR0307797-7A BR0307797A BR0307797A BR 0307797 A BR0307797 A BR 0307797A BR 0307797 A BR0307797 A BR 0307797A BR 0307797 A BR0307797 A BR 0307797A
Authority
BR
Brazil
Prior art keywords
polynucleotides
present
plants
methods
grown under
Prior art date
Application number
BR0307797-7A
Other languages
English (en)
Inventor
Hannah L Curtis
Thomas W Greene
Original Assignee
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida filed Critical Univ Florida
Publication of BR0307797A publication Critical patent/BR0307797A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01021—Starch synthase (2.4.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07027—Glucose-1-phosphate adenylyltransferase (2.7.7.27), i.e. ADP-glucose pyrophosphorylase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

"MUTANTES TERMOESTáVEIS DE ENZIMAS DA BIOSSìNTESE DE AMIDO". A presente invenção refere-se a novas moléculas de polinucleotídeos mutantes que codificam enzimas que têm aumentada estabilidade térmica. Estes polinucleotídeos, quando expressos em plantas, resultam em aumento do rendimento em plantas cultivadas sob condições de estresse térmico. As moléculas de polinucleotídeos da presente invenção codificam glicose pirofosforilase (AGP) de ADP de endosperma de milho e atividades de enzima sintase de amido solúvel (SSS). Plantas e tecido vegetal desenvolvidos para conter, ou transformados com, os polinucleotídeos mutantes, e expressando os polipeptídeos codificados pelos polinucleotídeos, também são contempladas pela presente invenção. A presente invenção também refere-se a métodos para isolar polinucleotídeos e polipeptídeos contemplados dentro do âmbito da invenção. Também são proporcionados métodos para aumentar o rendimento em plantes cultivadas sob condições de estresse térmico.
BR0307797-7A 2002-02-19 2003-02-18 Mutantes termoestáveis de enzimas da biossìntese de amido BR0307797A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/079,478 US6809235B2 (en) 1996-11-18 2002-02-19 Heat stable mutants of starch biosynthesis enzymes
PCT/US2003/004781 WO2003070901A2 (en) 2002-02-19 2003-02-18 Heat stable mutants of starch biosynthesis enzymes

Publications (1)

Publication Number Publication Date
BR0307797A true BR0307797A (pt) 2004-12-14

Family

ID=27752746

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0307797-7A BR0307797A (pt) 2002-02-19 2003-02-18 Mutantes termoestáveis de enzimas da biossìntese de amido

Country Status (14)

Country Link
US (2) US6809235B2 (pt)
EP (1) EP1482779B1 (pt)
JP (2) JP4310194B2 (pt)
CN (2) CN100482802C (pt)
AR (1) AR038574A1 (pt)
AT (1) ATE384431T1 (pt)
AU (1) AU2003217555B2 (pt)
BR (1) BR0307797A (pt)
CA (1) CA2475540A1 (pt)
DE (1) DE60318819D1 (pt)
ES (1) ES2299690T3 (pt)
MX (1) MXPA04007999A (pt)
PT (1) PT1482779E (pt)
WO (1) WO2003070901A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0208079A (pt) 2001-03-14 2004-07-27 Univ Florida Mutantes termo-estáveis em enzimas de biossìntese de amido
AU2002360463A1 (en) 2001-12-03 2003-06-17 University Of Florida Research Foundation, Inc. Variants of adp-glucose pyrophosphorylase affecting phosphate sensitivity and other parameters
US8710298B2 (en) 2003-08-18 2014-04-29 University Of Florida Research Foundation, Inc. Heat stable variants of plant adenosine diphosphate glucose pyrophosphorylase small subunit
US9074193B2 (en) * 2008-04-09 2015-07-07 University Of Florida Research Foundation, Inc. Heat resistant plants and plant tissues and methods and materials for making and using same
EP2336332A3 (en) 2008-04-29 2011-11-09 Monsanto Technology LLC Genes and uses for plant enhancement
CN101580822B (zh) * 2008-05-14 2011-07-20 中国农业大学 一种adp-葡萄糖焦磷酸化酶突变体及其编码基因与应用
US8362321B2 (en) * 2009-02-02 2013-01-29 University Of Florida Research Foundation, Inc. Methods and materials for increasing starch biosynthesis in plants
US9650616B2 (en) 2014-05-19 2017-05-16 University Of Florida Research Foundation, Inc. Methods for increasing grain yield
CN104673923B (zh) * 2015-02-15 2016-10-05 中国农业科学院作物科学研究所 用于小麦淀粉合酶基因TaSSIV等位变异检测的引物SSIV-1b及其检测方法
CN106337056B (zh) * 2016-09-23 2018-10-02 中国热带农业科学院热带生物技术研究所 一种改良植物果实支链淀粉品质的基因及其编码产物与应用
CN108753915A (zh) * 2018-05-12 2018-11-06 内蒙古农业大学 谷子酶活性的测定方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013861A (en) 1989-05-26 2000-01-11 Zeneca Limited Plants and processes for obtaining them
ES2217254T3 (es) 1992-10-14 2004-11-01 Syngenta Limited Nuevas plantas y procesos para obtenerlas.
GB9223454D0 (en) 1992-11-09 1992-12-23 Ici Plc Novel plants and processes for obtaining them
GB9307408D0 (en) 1993-04-08 1993-06-02 Danisco Transgenic plants
GB9412018D0 (en) 1994-06-16 1994-08-03 Cambridge Advanced Tech Modification of starch content in plants
DE69737951T2 (de) 1996-11-18 2007-11-15 University of Florida Research Foundation, Inc., Gainesville Hitzestabile mutanten von enzymen der stärkebiosynthese
US6403863B1 (en) * 1996-11-18 2002-06-11 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes
WO1999058698A2 (en) * 1998-05-14 1999-11-18 University Of Florida Heat stable mutants of starch biosynthesis enzymes
BR0208079A (pt) * 2001-03-14 2004-07-27 Univ Florida Mutantes termo-estáveis em enzimas de biossìntese de amido

Also Published As

Publication number Publication date
EP1482779A4 (en) 2005-12-14
JP4310194B2 (ja) 2009-08-05
DE60318819D1 (de) 2008-03-13
JP2005517434A (ja) 2005-06-16
US7312378B2 (en) 2007-12-25
US20020194642A1 (en) 2002-12-19
AR038574A1 (es) 2005-01-19
CN100482802C (zh) 2009-04-29
CA2475540A1 (en) 2003-08-28
US6809235B2 (en) 2004-10-26
MXPA04007999A (es) 2004-11-26
CN1638622A (zh) 2005-07-13
AU2003217555B2 (en) 2008-02-28
ATE384431T1 (de) 2008-02-15
PT1482779E (pt) 2008-04-18
CN101555481A (zh) 2009-10-14
ES2299690T3 (es) 2008-06-01
WO2003070901A3 (en) 2004-03-04
US20050120413A1 (en) 2005-06-02
AU2003217555A1 (en) 2003-09-09
WO2003070901A2 (en) 2003-08-28
JP2009106293A (ja) 2009-05-21
EP1482779A2 (en) 2004-12-08
EP1482779B1 (en) 2008-01-23
AU2003217555A2 (en) 2003-09-09

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: NAO APRESENTADA A GUIA DE CUMPRIMENTO DE EXIGENCIA. REFERENTE AS 8A E 9A ANUIDADES.