ATE384431T1 - Hitzestabile mutanten von stärkebiosyntheseenzymen - Google Patents

Hitzestabile mutanten von stärkebiosyntheseenzymen

Info

Publication number
ATE384431T1
ATE384431T1 AT03713507T AT03713507T ATE384431T1 AT E384431 T1 ATE384431 T1 AT E384431T1 AT 03713507 T AT03713507 T AT 03713507T AT 03713507 T AT03713507 T AT 03713507T AT E384431 T1 ATE384431 T1 AT E384431T1
Authority
AT
Austria
Prior art keywords
zymes
heat stable
starch biosynthesis
stable mutants
mutants
Prior art date
Application number
AT03713507T
Other languages
English (en)
Inventor
L Curtis Hannah
Thomas Greene
Original Assignee
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida filed Critical Univ Florida
Application granted granted Critical
Publication of ATE384431T1 publication Critical patent/ATE384431T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01021Starch synthase (2.4.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07027Glucose-1-phosphate adenylyltransferase (2.7.7.27), i.e. ADP-glucose pyrophosphorylase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
AT03713507T 2002-02-19 2003-02-18 Hitzestabile mutanten von stärkebiosyntheseenzymen ATE384431T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/079,478 US6809235B2 (en) 1996-11-18 2002-02-19 Heat stable mutants of starch biosynthesis enzymes

Publications (1)

Publication Number Publication Date
ATE384431T1 true ATE384431T1 (de) 2008-02-15

Family

ID=27752746

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03713507T ATE384431T1 (de) 2002-02-19 2003-02-18 Hitzestabile mutanten von stärkebiosyntheseenzymen

Country Status (14)

Country Link
US (2) US6809235B2 (de)
EP (1) EP1482779B1 (de)
JP (2) JP4310194B2 (de)
CN (2) CN101555481A (de)
AR (1) AR038574A1 (de)
AT (1) ATE384431T1 (de)
AU (1) AU2003217555B2 (de)
BR (1) BR0307797A (de)
CA (1) CA2475540A1 (de)
DE (1) DE60318819D1 (de)
ES (1) ES2299690T3 (de)
MX (1) MXPA04007999A (de)
PT (1) PT1482779E (de)
WO (1) WO2003070901A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR032990A1 (es) 2001-03-14 2003-12-03 Univ Florida Mutantes termoestables de enzimas para la biosintesis de almidon
EP2186900A3 (de) 2001-12-03 2010-06-02 University Of Florida Research Foundation, Inc. Die Phosphatempfindlichkeit und andere Parameter beeinflussende ADP-Glucose-Pyrophosphorylase-Varianten
US8710298B2 (en) 2003-08-18 2014-04-29 University Of Florida Research Foundation, Inc. Heat stable variants of plant adenosine diphosphate glucose pyrophosphorylase small subunit
US9074193B2 (en) * 2008-04-09 2015-07-07 University Of Florida Research Foundation, Inc. Heat resistant plants and plant tissues and methods and materials for making and using same
WO2009134339A2 (en) * 2008-04-29 2009-11-05 Monsanto Technology, Llc Genes and uses for plant enhancement
CN101580822B (zh) * 2008-05-14 2011-07-20 中国农业大学 一种adp-葡萄糖焦磷酸化酶突变体及其编码基因与应用
US8362321B2 (en) * 2009-02-02 2013-01-29 University Of Florida Research Foundation, Inc. Methods and materials for increasing starch biosynthesis in plants
US9650616B2 (en) 2014-05-19 2017-05-16 University Of Florida Research Foundation, Inc. Methods for increasing grain yield
CN104673923B (zh) * 2015-02-15 2016-10-05 中国农业科学院作物科学研究所 用于小麦淀粉合酶基因TaSSIV等位变异检测的引物SSIV-1b及其检测方法
CN106337056B (zh) * 2016-09-23 2018-10-02 中国热带农业科学院热带生物技术研究所 一种改良植物果实支链淀粉品质的基因及其编码产物与应用
CN108753915A (zh) * 2018-05-12 2018-11-06 内蒙古农业大学 谷子酶活性的测定方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013861A (en) 1989-05-26 2000-01-11 Zeneca Limited Plants and processes for obtaining them
CA2146998A1 (en) 1992-10-14 1994-04-28 Colin Roger Bird Novel plants and processes for obtaining them
GB9223454D0 (en) 1992-11-09 1992-12-23 Ici Plc Novel plants and processes for obtaining them
GB9307408D0 (en) 1993-04-08 1993-06-02 Danisco Transgenic plants
GB9412018D0 (en) 1994-06-16 1994-08-03 Cambridge Advanced Tech Modification of starch content in plants
US6403863B1 (en) * 1996-11-18 2002-06-11 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes
DE69737951T2 (de) 1996-11-18 2007-11-15 University of Florida Research Foundation, Inc., Gainesville Hitzestabile mutanten von enzymen der stärkebiosynthese
AU3993299A (en) * 1998-05-14 1999-11-29 University Of Florida Heat stable mutants of starch biosynthesis enzymes
AR032990A1 (es) * 2001-03-14 2003-12-03 Univ Florida Mutantes termoestables de enzimas para la biosintesis de almidon

Also Published As

Publication number Publication date
JP2005517434A (ja) 2005-06-16
EP1482779A2 (de) 2004-12-08
AU2003217555A1 (en) 2003-09-09
US6809235B2 (en) 2004-10-26
CN1638622A (zh) 2005-07-13
AU2003217555B2 (en) 2008-02-28
JP2009106293A (ja) 2009-05-21
WO2003070901A3 (en) 2004-03-04
US20020194642A1 (en) 2002-12-19
CN101555481A (zh) 2009-10-14
US20050120413A1 (en) 2005-06-02
AR038574A1 (es) 2005-01-19
ES2299690T3 (es) 2008-06-01
CA2475540A1 (en) 2003-08-28
US7312378B2 (en) 2007-12-25
WO2003070901A2 (en) 2003-08-28
EP1482779A4 (de) 2005-12-14
DE60318819D1 (de) 2008-03-13
JP4310194B2 (ja) 2009-08-05
MXPA04007999A (es) 2004-11-26
CN100482802C (zh) 2009-04-29
AU2003217555A2 (en) 2003-09-09
EP1482779B1 (de) 2008-01-23
BR0307797A (pt) 2004-12-14
PT1482779E (pt) 2008-04-18

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