DE60318819D1 - Hitzestabile mutanten von stärkebiosyntheseenzymen - Google Patents

Hitzestabile mutanten von stärkebiosyntheseenzymen

Info

Publication number
DE60318819D1
DE60318819D1 DE60318819T DE60318819T DE60318819D1 DE 60318819 D1 DE60318819 D1 DE 60318819D1 DE 60318819 T DE60318819 T DE 60318819T DE 60318819 T DE60318819 T DE 60318819T DE 60318819 D1 DE60318819 D1 DE 60318819D1
Authority
DE
Germany
Prior art keywords
hitzestabile
biosyntheseenzymen
mutants
strength
strength biosyntheseenzymen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60318819T
Other languages
English (en)
Inventor
L Curtis Hannah
Thomas W Greene
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida
University of Florida Research Foundation Inc
Original Assignee
University of Florida
University of Florida Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Florida, University of Florida Research Foundation Inc filed Critical University of Florida
Application granted granted Critical
Publication of DE60318819D1 publication Critical patent/DE60318819D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01021—Starch synthase (2.4.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07027—Glucose-1-phosphate adenylyltransferase (2.7.7.27), i.e. ADP-glucose pyrophosphorylase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
DE60318819T 2002-02-19 2003-02-18 Hitzestabile mutanten von stärkebiosyntheseenzymen Expired - Fee Related DE60318819D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/079,478 US6809235B2 (en) 1996-11-18 2002-02-19 Heat stable mutants of starch biosynthesis enzymes
PCT/US2003/004781 WO2003070901A2 (en) 2002-02-19 2003-02-18 Heat stable mutants of starch biosynthesis enzymes

Publications (1)

Publication Number Publication Date
DE60318819D1 true DE60318819D1 (de) 2008-03-13

Family

ID=27752746

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60318819T Expired - Fee Related DE60318819D1 (de) 2002-02-19 2003-02-18 Hitzestabile mutanten von stärkebiosyntheseenzymen

Country Status (14)

Country Link
US (2) US6809235B2 (de)
EP (1) EP1482779B1 (de)
JP (2) JP4310194B2 (de)
CN (2) CN100482802C (de)
AR (1) AR038574A1 (de)
AT (1) ATE384431T1 (de)
AU (1) AU2003217555B2 (de)
BR (1) BR0307797A (de)
CA (1) CA2475540A1 (de)
DE (1) DE60318819D1 (de)
ES (1) ES2299690T3 (de)
MX (1) MXPA04007999A (de)
PT (1) PT1482779E (de)
WO (1) WO2003070901A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002072784A2 (en) 2001-03-14 2002-09-19 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes
BR0214670A (pt) 2001-12-03 2005-05-03 Univ Florida Variantes da adp-glicose pirofosforilase que afetam a sensibilidade do fosfato e outros parâmetros
WO2005019425A2 (en) 2003-08-18 2005-03-03 University Of Florida Research Foundation, Inc. Heat stable variants of adenosine diphosphate glucose pyrophosphorylase
US9074193B2 (en) * 2008-04-09 2015-07-07 University Of Florida Research Foundation, Inc. Heat resistant plants and plant tissues and methods and materials for making and using same
EP2271760A2 (de) 2008-04-29 2011-01-12 Monsanto Technology LLC Gene und ihre verwendung zur pflanzenverbesserung
CN101580822B (zh) * 2008-05-14 2011-07-20 中国农业大学 一种adp-葡萄糖焦磷酸化酶突变体及其编码基因与应用
US8362321B2 (en) * 2009-02-02 2013-01-29 University Of Florida Research Foundation, Inc. Methods and materials for increasing starch biosynthesis in plants
CN106459933B (zh) 2014-05-19 2021-05-18 佛罗里达大学研究基金公司 用于增加谷物产量的方法
CN104673923B (zh) * 2015-02-15 2016-10-05 中国农业科学院作物科学研究所 用于小麦淀粉合酶基因TaSSIV等位变异检测的引物SSIV-1b及其检测方法
CN106337056B (zh) * 2016-09-23 2018-10-02 中国热带农业科学院热带生物技术研究所 一种改良植物果实支链淀粉品质的基因及其编码产物与应用
CN108753915A (zh) * 2018-05-12 2018-11-06 内蒙古农业大学 谷子酶活性的测定方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013861A (en) 1989-05-26 2000-01-11 Zeneca Limited Plants and processes for obtaining them
ES2217254T3 (es) 1992-10-14 2004-11-01 Syngenta Limited Nuevas plantas y procesos para obtenerlas.
GB9223454D0 (en) 1992-11-09 1992-12-23 Ici Plc Novel plants and processes for obtaining them
GB9307408D0 (en) 1993-04-08 1993-06-02 Danisco Transgenic plants
GB9412018D0 (en) 1994-06-16 1994-08-03 Cambridge Advanced Tech Modification of starch content in plants
US6403863B1 (en) * 1996-11-18 2002-06-11 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes
US6069300A (en) 1996-11-18 2000-05-30 University Of Florida Heat stable mutants of starch biosynthesis enzymes
WO1999058698A2 (en) * 1998-05-14 1999-11-18 University Of Florida Heat stable mutants of starch biosynthesis enzymes
WO2002072784A2 (en) * 2001-03-14 2002-09-19 University Of Florida Research Foundation, Inc. Heat stable mutants of starch biosynthesis enzymes

Also Published As

Publication number Publication date
US6809235B2 (en) 2004-10-26
PT1482779E (pt) 2008-04-18
WO2003070901A2 (en) 2003-08-28
US7312378B2 (en) 2007-12-25
AU2003217555A1 (en) 2003-09-09
EP1482779B1 (de) 2008-01-23
US20020194642A1 (en) 2002-12-19
AU2003217555B2 (en) 2008-02-28
JP2005517434A (ja) 2005-06-16
JP2009106293A (ja) 2009-05-21
US20050120413A1 (en) 2005-06-02
EP1482779A2 (de) 2004-12-08
BR0307797A (pt) 2004-12-14
AR038574A1 (es) 2005-01-19
AU2003217555A2 (en) 2003-09-09
CN100482802C (zh) 2009-04-29
CN1638622A (zh) 2005-07-13
JP4310194B2 (ja) 2009-08-05
ATE384431T1 (de) 2008-02-15
WO2003070901A3 (en) 2004-03-04
CN101555481A (zh) 2009-10-14
ES2299690T3 (es) 2008-06-01
EP1482779A4 (de) 2005-12-14
MXPA04007999A (es) 2004-11-26
CA2475540A1 (en) 2003-08-28

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Legal Events

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8339 Ceased/non-payment of the annual fee