BR0215191A - Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo - Google Patents

Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo

Info

Publication number
BR0215191A
BR0215191A BR0215191-0A BR0215191A BR0215191A BR 0215191 A BR0215191 A BR 0215191A BR 0215191 A BR0215191 A BR 0215191A BR 0215191 A BR0215191 A BR 0215191A
Authority
BR
Brazil
Prior art keywords
csf
biologically active
purification
granulocyte colony
stimulation factor
Prior art date
Application number
BR0215191-0A
Other languages
English (en)
Inventor
Vladka Gaberc Porekar
Viktor Menart
Original Assignee
Lek Pharmaceuticals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lek Pharmaceuticals filed Critical Lek Pharmaceuticals
Publication of BR0215191A publication Critical patent/BR0215191A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/53Colony-stimulating factor [CSF]
    • C07K14/535Granulocyte CSF; Granulocyte-macrophage CSF
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19Cytokines; Lymphokines; Interferons
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19Cytokines; Lymphokines; Interferons
    • A61K38/193Colony stimulating factors [CSF]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Pain & Pain Management (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Rheumatology (AREA)
  • Dermatology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

"PROCESSO PARA A PURIFICAçãO E/OU ISOLAMENTO DE FATOR DE ESTIMULAçãO DE COLÈNIA DE GRANULóCITO BIOLOGICAMENTE ATIVO". A presente invenção refere-se ao processo para o isolamento de fator de estimulação de colónia de granulócito biologicamente ativo (G-CSF), o qual permite a separação de moléculas monoméricas biologicamente ativas corretamente dobradas de G-CSF das moléculas incorretamente dobradas, biologicamente inativas monoméricas, oligo- ou poliméricas e também das agregadas de G-CSF empregando-se a cromatografia de afinidade de metal imobilizado. O processo da invenção, se desejado o processo inteiro, pode ser vantajosamente executado sob condições naturais. O G-CSF biologicamente ativo com uma pureza maior do que 95% é desse modo obtido. Somente duas etapas cromatográficas adicionais, cromatografia de permuta catiónica e filtragem de gel, são em seguida de preferência aplicadas para remover os traços de impurezas. O processo inteiro resulta na produção de rendimentos elevados de G-CSF com uma pureza maior do que 99%. O processo descrito é particularmente adequado para a produção industrial de G-CSF.
BR0215191-0A 2001-12-19 2002-12-05 Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo BR0215191A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SI200100322A SI21102A (sl) 2001-12-19 2001-12-19 Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika
PCT/EP2002/013810 WO2003051922A1 (en) 2001-12-19 2002-12-05 Process for the purification and/or isolation of biologically active granulocyte colony stimulating factor

Publications (1)

Publication Number Publication Date
BR0215191A true BR0215191A (pt) 2004-11-16

Family

ID=20433022

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0215191-0A BR0215191A (pt) 2001-12-19 2002-12-05 Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo

Country Status (22)

Country Link
US (1) US20050159589A1 (pt)
EP (2) EP1458757B1 (pt)
JP (1) JP4454311B2 (pt)
KR (1) KR20040071212A (pt)
CN (1) CN1606568A (pt)
AT (1) ATE423136T1 (pt)
AU (1) AU2002366275B2 (pt)
BR (1) BR0215191A (pt)
CA (1) CA2469984C (pt)
DE (1) DE60231243D1 (pt)
HK (1) HK1064681A1 (pt)
HR (1) HRP20040572A2 (pt)
HU (1) HUP0402547A3 (pt)
IL (2) IL162448A0 (pt)
MX (1) MXPA04006076A (pt)
NO (1) NO20042967L (pt)
NZ (1) NZ533305A (pt)
PL (1) PL207775B1 (pt)
RU (1) RU2358980C2 (pt)
SI (2) SI21102A (pt)
WO (1) WO2003051922A1 (pt)
ZA (1) ZA200404291B (pt)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI21273A (sl) * 2002-07-31 2004-02-29 LEK farmacevtska dru�ba d.d. Priprava inkluzijskih teles z visokim deležem pravilno zvitega prekurzorja heterolognega proteina
ATE322506T1 (de) * 2004-01-19 2006-04-15 Ares Trading Sa Verfahren zur reinigung von in bakterien exprimierten proteinen
EP1803464A4 (en) * 2004-09-17 2009-09-09 Cellgentech Inc TOPICAL PREPARATION FOR THE TREATMENT OF SKIN DISEASES
WO2006086822A1 (en) * 2005-02-15 2006-08-24 Apollo Life Sciences Limited A molecule and chimeric molecules thereof
US20080171857A1 (en) * 2005-03-17 2008-07-17 Uma Devi Komath Process for the Purification of Recombinant Granulocyte-Colony Stimulating Factor
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE202006020194U1 (de) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
EP1878739A1 (en) * 2006-07-14 2008-01-16 LEK Pharmaceuticals D.D. One step IMAC (MCAC) purification of proteins
WO2008096370A2 (en) * 2007-02-05 2008-08-14 Natco Pharma Limited An efficient and novel purification method of recombinant hg-csf
PT2197919E (pt) 2007-08-27 2014-07-17 Ratiopharm Gmbh Formulação líquida de conjugado de g-csf
JP2009073819A (ja) * 2007-08-29 2009-04-09 Fujifilm Corp 生理活性物質の精製方法
US20120093765A1 (en) * 2009-06-16 2012-04-19 Lupin Limited Process for purification of recombinant human granulocyte colony stimulating factor
MX2011013417A (es) 2009-06-25 2012-03-29 Amgen Inc Procesos de purificacion por captura para proteinas expresadas en un sistema no mamifero.
ES2670827T3 (es) * 2010-03-30 2018-06-01 Octapharma Ag Proceso para la purificación del factor estimulador de colonias de granulocitos, G-CSF
SG193545A1 (en) 2011-03-29 2013-11-29 Glaxosmithkline Llc Buffer system for protein purification
CN103215340A (zh) * 2012-01-19 2013-07-24 江苏粒福特生物科技有限公司 一种新颖的粒细胞制备及其抗癌活性检测方法
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
HUP1200172A2 (en) * 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61227526A (ja) 1984-07-25 1986-10-09 Chugai Pharmaceut Co Ltd 新規なコロニー刺激因子
WO1986004605A1 (en) 1985-02-08 1986-08-14 Chugai Seiyaku Kabushiki Kaisha Human granulocyte colony stimulating factor
US4810643A (en) 1985-08-23 1989-03-07 Kirin- Amgen Inc. Production of pluripotent granulocyte colony-stimulating factor
DK203187A (da) 1986-04-22 1987-10-23 Immunex Corp Human g-csf proteinekspression
US5055555A (en) 1989-01-05 1991-10-08 Helmut Sassenfeld Purification of G-CSF
US5169936A (en) 1989-04-14 1992-12-08 Biogen, Inc. Protein purification on immobilized metal affinity resins effected by elution using a weak ligand
US5932102A (en) 1998-01-12 1999-08-03 Schering Corporation Immobilized metal, affinity chromatography
FR2796071B1 (fr) 1999-07-08 2001-09-07 Hoechst Marion Roussel Inc Procede de purification de facteur de stimulation de colonies de granulocytes

Also Published As

Publication number Publication date
HRP20040572A2 (en) 2005-06-30
HUP0402547A3 (en) 2010-01-28
JP4454311B2 (ja) 2010-04-21
RU2004121982A (ru) 2005-04-20
KR20040071212A (ko) 2004-08-11
IL162448A0 (en) 2005-11-20
DE60231243D1 (de) 2009-04-02
ATE423136T1 (de) 2009-03-15
WO2003051922A1 (en) 2003-06-26
AU2002366275B2 (en) 2009-04-02
AU2002366275A1 (en) 2003-06-30
US20050159589A1 (en) 2005-07-21
MXPA04006076A (es) 2005-02-24
NO20042967L (no) 2004-09-07
CA2469984A1 (en) 2003-06-26
EP1458757B1 (en) 2009-02-18
SI21102A (sl) 2003-06-30
HUP0402547A2 (hu) 2005-03-29
PL369669A1 (en) 2005-05-02
CN1606568A (zh) 2005-04-13
PL207775B1 (pl) 2011-01-31
SI1458757T1 (sl) 2009-10-31
EP2053061A1 (en) 2009-04-29
HK1064681A1 (en) 2005-02-04
RU2358980C2 (ru) 2009-06-20
NZ533305A (en) 2005-11-25
EP1458757A1 (en) 2004-09-22
IL162448A (en) 2010-12-30
CA2469984C (en) 2014-05-20
ZA200404291B (en) 2005-08-05
JP2005525304A (ja) 2005-08-25

Similar Documents

Publication Publication Date Title
BR0215191A (pt) Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo
NO20050520L (no) Rensingav N,N-dimetylacetamdi
BR0308385B1 (pt) processo para purificar uma composição compreendendo ácido (met)acrílico, aparelho para produzir ácido (met)acrílico e aparelho para polimerizar ácido (met)acrílico
ATE226960T1 (de) Verfahren zur herstellung von cardiodilatin- fragmenten, hochgereinigte cardiodilatin- fragmente und zwischenprodukte zu deren herstellung
WO2010147686A3 (en) Methods of purifying small modular immunopharmaceutical proteins
BR9713055A (pt) Processo para isolar uma neurotrofina humana recombinante, composição de neurotrofina e processo para purificar uma neurotrofina
BR0309659A (pt) Método para produzir vìrus de uma cultura de células, composição, e, método para produzir reovìrus infeccioso
DK0731812T3 (da) Fremgangsmåde til isolering af Poly-beta-1-4-N-Acetylglucosamin
BRPI0414764B8 (pt) método para purificar ácido succínico à partir de caldo de fermentação
SE9603068D0 (sv) Process for purifying a protein
BG65748B1 (bg) Метод за пречистване на интерферон
PT95830A (pt) Processo para o isolamento do factor viii sob a forma de uma fraccao pura directamente de plasma mediante filtracao em gel
JPH0586097A (ja) 上清iv、特にiv−4またはコーンフラクシヨンvまたはそれと類似の上清または画分からヒトアルブミンを単離する方法
EP2409995A3 (en) Reduction of endotoxin in polysialic acids
ATE235548T1 (de) Methode für die reinigung kovalent geschlossener zirkulärer dns
ATE150025T1 (de) Verfahren zur herstellung und reinigung von phospholipidmischungen frei von kontaminierenden unkonventionellen viren
BRPI0517800A (pt) processo para preparar um polìmero de alginato modificado, polìmero de alginato modificado, e, gel ou fibra de alginato
BRPI0511893A (pt) processo para isolamento de proteìna
WO2004009632A3 (de) Verfahren zur herstellung eines immunstimulatorischen muzins (muc1)
BR0007528A (pt) Métodos para clarificação de soluções de açúcar
DE60327598D1 (de) Trennverfahren mit aromatischen thioetherliganden
Weiss et al. Separation of long-chain bases by thin-layer chromatography; instability of sphingosine
ATE540974T1 (de) Reinigung des rekombinanten menschlichen faktors xiii
Wang et al. Mass spectrometry of L‐β‐aspartamido carbohydrates isolated from ovalbumin
TW200519123A (en) A novel process to recover and purify acarbose from fermentation broth

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]