BR0215191A - Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo - Google Patents
Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativoInfo
- Publication number
- BR0215191A BR0215191A BR0215191-0A BR0215191A BR0215191A BR 0215191 A BR0215191 A BR 0215191A BR 0215191 A BR0215191 A BR 0215191A BR 0215191 A BR0215191 A BR 0215191A
- Authority
- BR
- Brazil
- Prior art keywords
- csf
- biologically active
- purification
- granulocyte colony
- stimulation factor
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 7
- 210000003714 granulocyte Anatomy 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 230000000638 stimulation Effects 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 title 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 abstract 7
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 abstract 7
- 238000005277 cation exchange chromatography Methods 0.000 abstract 1
- 238000011210 chromatographic step Methods 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 238000001597 immobilized metal affinity chromatography Methods 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000009413 insulation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/193—Colony stimulating factors [CSF]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Epidemiology (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Oncology (AREA)
- Pain & Pain Management (AREA)
- Dermatology (AREA)
- Communicable Diseases (AREA)
- Rheumatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
"PROCESSO PARA A PURIFICAçãO E/OU ISOLAMENTO DE FATOR DE ESTIMULAçãO DE COLÈNIA DE GRANULóCITO BIOLOGICAMENTE ATIVO". A presente invenção refere-se ao processo para o isolamento de fator de estimulação de colónia de granulócito biologicamente ativo (G-CSF), o qual permite a separação de moléculas monoméricas biologicamente ativas corretamente dobradas de G-CSF das moléculas incorretamente dobradas, biologicamente inativas monoméricas, oligo- ou poliméricas e também das agregadas de G-CSF empregando-se a cromatografia de afinidade de metal imobilizado. O processo da invenção, se desejado o processo inteiro, pode ser vantajosamente executado sob condições naturais. O G-CSF biologicamente ativo com uma pureza maior do que 95% é desse modo obtido. Somente duas etapas cromatográficas adicionais, cromatografia de permuta catiónica e filtragem de gel, são em seguida de preferência aplicadas para remover os traços de impurezas. O processo inteiro resulta na produção de rendimentos elevados de G-CSF com uma pureza maior do que 99%. O processo descrito é particularmente adequado para a produção industrial de G-CSF.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SI200100322A SI21102A (sl) | 2001-12-19 | 2001-12-19 | Postopek za izolacijo biološko aktivnega granulocitne kolonije stimulirajočega dejavnika |
| PCT/EP2002/013810 WO2003051922A1 (en) | 2001-12-19 | 2002-12-05 | Process for the purification and/or isolation of biologically active granulocyte colony stimulating factor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0215191A true BR0215191A (pt) | 2004-11-16 |
Family
ID=20433022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0215191-0A BR0215191A (pt) | 2001-12-19 | 2002-12-05 | Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US20050159589A1 (pt) |
| EP (2) | EP2053061A1 (pt) |
| JP (1) | JP4454311B2 (pt) |
| KR (1) | KR20040071212A (pt) |
| CN (1) | CN1606568A (pt) |
| AT (1) | ATE423136T1 (pt) |
| AU (1) | AU2002366275B2 (pt) |
| BR (1) | BR0215191A (pt) |
| CA (1) | CA2469984C (pt) |
| DE (1) | DE60231243D1 (pt) |
| HK (1) | HK1064681A1 (pt) |
| HR (1) | HRP20040572A2 (pt) |
| HU (1) | HUP0402547A3 (pt) |
| IL (2) | IL162448A0 (pt) |
| MX (1) | MXPA04006076A (pt) |
| NO (1) | NO20042967L (pt) |
| NZ (1) | NZ533305A (pt) |
| PL (1) | PL207775B1 (pt) |
| RU (1) | RU2358980C2 (pt) |
| SI (2) | SI21102A (pt) |
| WO (1) | WO2003051922A1 (pt) |
| ZA (1) | ZA200404291B (pt) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SI21273A (sl) * | 2002-07-31 | 2004-02-29 | LEK farmacevtska dru�ba d.d. | Priprava inkluzijskih teles z visokim deležem pravilno zvitega prekurzorja heterolognega proteina |
| SI1439191T1 (sl) * | 2004-01-19 | 2006-06-30 | Ares Trading Sa | Postopek ciscenja proteinov izrazenih v bakteriji |
| WO2006030887A1 (ja) * | 2004-09-17 | 2006-03-23 | Cellgentech, Inc. | 外用皮膚潰瘍治療剤 |
| CA2597795A1 (en) * | 2005-02-15 | 2006-08-24 | Apollo Life Sciences Limited | A molecule and chimeric molecules thereof |
| EP1869078A2 (en) * | 2005-03-17 | 2007-12-26 | Zenotech Laboratories Limited | Process for the purification of recombinant granulocyte-colony stimulating factor |
| DE102005033250A1 (de) | 2005-07-15 | 2007-01-18 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von G-CSF |
| DE102006009437A1 (de) | 2006-03-01 | 2007-09-13 | Bioceuticals Arzneimittel Ag | G-CSF-Flüssigformulierung |
| EP1878739A1 (en) | 2006-07-14 | 2008-01-16 | LEK Pharmaceuticals D.D. | One step IMAC (MCAC) purification of proteins |
| WO2008096370A2 (en) * | 2007-02-05 | 2008-08-14 | Natco Pharma Limited | An efficient and novel purification method of recombinant hg-csf |
| JP5570988B2 (ja) | 2007-08-27 | 2014-08-13 | ラティオファーム ゲーエムベーハー | Ｇ−ｃｓｆ結合体の液体調製物 |
| JP2009073819A (ja) * | 2007-08-29 | 2009-04-09 | Fujifilm Corp | 生理活性物質の精製方法 |
| JP2012530131A (ja) * | 2009-06-16 | 2012-11-29 | ルピン・リミテッド | 組み換えヒト顆粒球コロニー刺激因子の精製のための方法 |
| MX2011013417A (es) | 2009-06-25 | 2012-03-29 | Amgen Inc | Procesos de purificacion por captura para proteinas expresadas en un sistema no mamifero. |
| US9453045B2 (en) * | 2010-03-30 | 2016-09-27 | Octapharma Ag | Process for the purification of a growth factor protein |
| CN107915769A (zh) | 2011-03-29 | 2018-04-17 | 葛兰素史密斯克莱有限责任公司 | 用于蛋白纯化的缓冲液体系 |
| CN103215340A (zh) * | 2012-01-19 | 2013-07-24 | 江苏粒福特生物科技有限公司 | 一种新颖的粒细胞制备及其抗癌活性检测方法 |
| HUP1200171A1 (hu) | 2012-03-19 | 2013-09-30 | Richter Gedeon Nyrt | Módszerek polipeptidek elõállítására |
| HUP1200172A2 (en) * | 2012-03-19 | 2013-10-28 | Richter Gedeon Nyrt | Methods for refolding g-csf from inclusion bodies |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61227526A (ja) | 1984-07-25 | 1986-10-09 | Chugai Pharmaceut Co Ltd | 新規なコロニー刺激因子 |
| EP0215126B1 (en) | 1985-02-08 | 1991-07-31 | Chugai Seiyaku Kabushiki Kaisha | Human granulocyte colony stimulating factor |
| US4810643A (en) | 1985-08-23 | 1989-03-07 | Kirin- Amgen Inc. | Production of pluripotent granulocyte colony-stimulating factor |
| DK203187A (da) | 1986-04-22 | 1987-10-23 | Immunex Corp | Human g-csf proteinekspression |
| US5055555A (en) | 1989-01-05 | 1991-10-08 | Helmut Sassenfeld | Purification of G-CSF |
| US5169936A (en) | 1989-04-14 | 1992-12-08 | Biogen, Inc. | Protein purification on immobilized metal affinity resins effected by elution using a weak ligand |
| US5932102A (en) | 1998-01-12 | 1999-08-03 | Schering Corporation | Immobilized metal, affinity chromatography |
| FR2796071B1 (fr) * | 1999-07-08 | 2001-09-07 | Hoechst Marion Roussel Inc | Procede de purification de facteur de stimulation de colonies de granulocytes |
-
2001
- 2001-12-19 SI SI200100322A patent/SI21102A/sl not_active IP Right Cessation
-
2002
- 2002-12-05 AT AT02804876T patent/ATE423136T1/de active
- 2002-12-05 PL PL369669A patent/PL207775B1/pl unknown
- 2002-12-05 EP EP09152902A patent/EP2053061A1/en not_active Withdrawn
- 2002-12-05 DE DE60231243T patent/DE60231243D1/de not_active Expired - Lifetime
- 2002-12-05 US US10/499,315 patent/US20050159589A1/en not_active Abandoned
- 2002-12-05 RU RU2004121982/13A patent/RU2358980C2/ru not_active IP Right Cessation
- 2002-12-05 MX MXPA04006076A patent/MXPA04006076A/es active IP Right Grant
- 2002-12-05 CA CA2469984A patent/CA2469984C/en not_active Expired - Fee Related
- 2002-12-05 NZ NZ533305A patent/NZ533305A/en unknown
- 2002-12-05 AU AU2002366275A patent/AU2002366275B2/en not_active Ceased
- 2002-12-05 EP EP02804876A patent/EP1458757B1/en not_active Expired - Lifetime
- 2002-12-05 IL IL16244802A patent/IL162448A0/xx unknown
- 2002-12-05 WO PCT/EP2002/013810 patent/WO2003051922A1/en active IP Right Grant
- 2002-12-05 JP JP2003552802A patent/JP4454311B2/ja not_active Expired - Fee Related
- 2002-12-05 KR KR10-2004-7009409A patent/KR20040071212A/ko not_active Application Discontinuation
- 2002-12-05 HU HU0402547A patent/HUP0402547A3/hu unknown
- 2002-12-05 CN CNA028254651A patent/CN1606568A/zh active Pending
- 2002-12-05 SI SI200230822T patent/SI1458757T1/sl unknown
- 2002-12-05 BR BR0215191-0A patent/BR0215191A/pt not_active Application Discontinuation
-
2004
- 2004-06-01 ZA ZA200404291A patent/ZA200404291B/en unknown
- 2004-06-10 IL IL162448A patent/IL162448A/en not_active IP Right Cessation
- 2004-06-18 HR HR20040572A patent/HRP20040572A2/xx not_active Application Discontinuation
- 2004-07-14 NO NO20042967A patent/NO20042967L/no not_active Application Discontinuation
- 2004-09-23 HK HK04107314.0A patent/HK1064681A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0402547A2 (hu) | 2005-03-29 |
| CA2469984A1 (en) | 2003-06-26 |
| PL207775B1 (pl) | 2011-01-31 |
| PL369669A1 (en) | 2005-05-02 |
| NO20042967L (no) | 2004-09-07 |
| MXPA04006076A (es) | 2005-02-24 |
| CA2469984C (en) | 2014-05-20 |
| ATE423136T1 (de) | 2009-03-15 |
| WO2003051922A1 (en) | 2003-06-26 |
| SI1458757T1 (sl) | 2009-10-31 |
| RU2358980C2 (ru) | 2009-06-20 |
| KR20040071212A (ko) | 2004-08-11 |
| AU2002366275B2 (en) | 2009-04-02 |
| RU2004121982A (ru) | 2005-04-20 |
| EP2053061A1 (en) | 2009-04-29 |
| SI21102A (sl) | 2003-06-30 |
| CN1606568A (zh) | 2005-04-13 |
| HK1064681A1 (en) | 2005-02-04 |
| HUP0402547A3 (en) | 2010-01-28 |
| NZ533305A (en) | 2005-11-25 |
| AU2002366275A1 (en) | 2003-06-30 |
| JP4454311B2 (ja) | 2010-04-21 |
| DE60231243D1 (de) | 2009-04-02 |
| EP1458757A1 (en) | 2004-09-22 |
| HRP20040572A2 (en) | 2005-06-30 |
| ZA200404291B (en) | 2005-08-05 |
| IL162448A (en) | 2010-12-30 |
| US20050159589A1 (en) | 2005-07-21 |
| EP1458757B1 (en) | 2009-02-18 |
| IL162448A0 (en) | 2005-11-20 |
| JP2005525304A (ja) | 2005-08-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR0215191A (pt) | Processo para a purificação e/ou isolamento de fator de estimulação de colÈnia de granulócito biologicamente ativo | |
| BR0308385B1 (pt) | processo para purificar uma composição compreendendo ácido (met)acrílico, aparelho para produzir ácido (met)acrílico e aparelho para polimerizar ácido (met)acrílico | |
| WO2010147686A3 (en) | Methods of purifying small modular immunopharmaceutical proteins | |
| Raftery et al. | Separation of glycosaminoglycan saccharide and glycoside mixtures by gel filtration | |
| JP3940037B2 (ja) | 陽イオン交換クロマトグラフィーを介する薬理学的に活性なタンパク質の精製方法 | |
| BR0309659A (pt) | Método para produzir vìrus de uma cultura de células, composição, e, método para produzir reovìrus infeccioso | |
| DK0731812T3 (da) | Fremgangsmåde til isolering af Poly-beta-1-4-N-Acetylglucosamin | |
| BRPI0414764B8 (pt) | método para purificar ácido succínico à partir de caldo de fermentação | |
| ES2155477T3 (es) | Purificacion cromatografica y procedimiento de separacion de acidos nucleicos. | |
| SE9603068D0 (sv) | Process for purifying a protein | |
| PT95830A (pt) | Processo para o isolamento do factor viii sob a forma de uma fraccao pura directamente de plasma mediante filtracao em gel | |
| JPH0586097A (ja) | 上清ｉｖ、特にｉｖ−４またはコーンフラクシヨンｖまたはそれと類似の上清または画分からヒトアルブミンを単離する方法 | |
| EP2409995A3 (en) | Reduction of endotoxin in polysialic acids | |
| ATE235548T1 (de) | Methode für die reinigung kovalent geschlossener zirkulärer dns | |
| BRPI0517800A (pt) | processo para preparar um polìmero de alginato modificado, polìmero de alginato modificado, e, gel ou fibra de alginato | |
| DE69308834D1 (de) | Verfahren zur herstellung und reinigung von phospholipidmischungen frei von kontaminierenden unkonventionellen viren | |
| Chu et al. | Quantitative separation of 4-hydroxyproline from skeletal muscle collagen by micellar electrokinetic capillary electrophoresis | |
| BRPI0511893A (pt) | processo para isolamento de proteìna | |
| BR0007528A (pt) | Métodos para clarificação de soluções de açúcar | |
| DE60327598D1 (de) | Trennverfahren mit aromatischen thioetherliganden | |
| Weiss et al. | Separation of long-chain bases by thin-layer chromatography; instability of sphingosine | |
| ATE540974T1 (de) | Reinigung des rekombinanten menschlichen faktors xiii | |
| WO2016028016A1 (ko) | D-아르기닌의 제조 방법 | |
| Wang et al. | Mass spectrometry of L‐β‐aspartamido carbohydrates isolated from ovalbumin | |
| KR930013133A (ko) | 5'-이노신산 함유액의 정제 방법 (i) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B11E | Dismissal acc. art. 34 of ipl - requirements for examination incomplete | ||
| B11T | Dismissal of application maintained [chapter 11.20 patent gazette] |