ATE235548T1 - Methode für die reinigung kovalent geschlossener zirkulärer dns - Google Patents

Methode für die reinigung kovalent geschlossener zirkulärer dns

Info

Publication number
ATE235548T1
ATE235548T1 AT99115046T AT99115046T ATE235548T1 AT E235548 T1 ATE235548 T1 AT E235548T1 AT 99115046 T AT99115046 T AT 99115046T AT 99115046 T AT99115046 T AT 99115046T AT E235548 T1 ATE235548 T1 AT E235548T1
Authority
AT
Austria
Prior art keywords
dna
ccc
covalently closed
closed circular
purifying
Prior art date
Application number
AT99115046T
Other languages
English (en)
Inventor
Stephan Wahle
Joachim Schorr
Martin Weber
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE235548T1 publication Critical patent/ATE235548T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT99115046T 1998-09-14 1999-08-04 Methode für die reinigung kovalent geschlossener zirkulärer dns ATE235548T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98117389A EP0987327A1 (de) 1998-09-14 1998-09-14 Methode für die Reinigung kovalent geschlossener zirkulärer DNS

Publications (1)

Publication Number Publication Date
ATE235548T1 true ATE235548T1 (de) 2003-04-15

Family

ID=8232629

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99115046T ATE235548T1 (de) 1998-09-14 1999-08-04 Methode für die reinigung kovalent geschlossener zirkulärer dns

Country Status (5)

Country Link
US (1) US6242220B1 (de)
EP (1) EP0987327A1 (de)
JP (1) JP3304932B2 (de)
AT (1) ATE235548T1 (de)
DE (1) DE69906210T2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0127809D0 (en) * 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
US20040002081A1 (en) * 2001-12-18 2004-01-01 Boehringer Ingelheim International Gmbh And Bia Separations D.O.O. Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
DE50202704D1 (de) * 2002-01-22 2005-05-12 Mpb Meltec Patent Und Beteilig Verfahren und Vorrichtung für die Probenvorbereitung zur Analyse von biologischen Proben
US8377715B2 (en) * 2003-07-14 2013-02-19 Phynexus, Inc. Method and device for sample preparation
US7846333B2 (en) 2003-11-24 2010-12-07 Effendorf AG Porous media
GB2416030B (en) * 2004-01-28 2008-07-23 Norchip As A diagnostic system for carrying out a nucleic acid sequence amplification and detection process
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
EA010864B1 (ru) * 2004-08-16 2008-12-30 Нейче Текнолоджи Корп. Улучшенные штаммы e.coli для продуцирования плазмидной днк
JP2006129861A (ja) * 2004-10-06 2006-05-25 Japan Science & Technology Agency 閉環状ｄｎａを簡易に調製する方法、キット及び装置
JP5492207B2 (ja) 2008-08-27 2014-05-14 ライフ テクノロジーズ コーポレーション 生物学的サンプルの処理装置および処理方法
US11235323B2 (en) 2008-08-27 2022-02-01 Life Technologies Corporation Apparatus for and method of processing biological samples
GB2473868A (en) 2009-09-28 2011-03-30 Invitrogen Dynal As Apparatus and method of automated processing of biological samples
US9580462B2 (en) 2010-09-01 2017-02-28 Invent Biotechnologies, Inc. Protein extraction methods
US8816064B2 (en) 2010-09-30 2014-08-26 Phynexus, Inc. Purification of nucleic acids
US9540635B2 (en) * 2012-05-09 2017-01-10 Bio-Rad Laboratories, Inc. Buffer for one-step DNA extraction
CN109266718A (zh) * 2018-10-19 2019-01-25 广东菲鹏生物有限公司 检测核酸内切酶存在的方法
WO2021126818A1 (en) 2019-12-18 2021-06-24 Life Technologies Corporation Systems, methods, and devices for automated nucleic acid and protein isolation
WO2023081777A1 (en) * 2021-11-05 2023-05-11 Cedars-Sinai Medical Center Kits and methods for quantitative assessment and enrichment of extrachromosomal circular dna

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6043383A (ja) * 1983-08-19 1985-03-07 Toyobo Co Ltd 環状ｄνａの調製方法
WO1992013963A1 (en) * 1991-01-30 1992-08-20 Hyman Edward D Method for preparation of closed circular dna
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
WO1995021178A1 (de) * 1994-02-07 1995-08-10 Qiagen Gmbh Steigerung der transfektionseffizienz von nucleinsäuren durch die verwendung von isopropanol in wässrigen lösungen

Also Published As

Publication number Publication date
EP0987327A1 (de) 2000-03-22
JP3304932B2 (ja) 2002-07-22
DE69906210D1 (de) 2003-04-30
JP2000093171A (ja) 2000-04-04
DE69906210T2 (de) 2004-02-12
US6242220B1 (en) 2001-06-05

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Legal Events

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