BR0213259A - Método para produzir um composto bioativo apresentando uma bioatividade conhecida, e, células recombinantes - Google Patents

Método para produzir um composto bioativo apresentando uma bioatividade conhecida, e, células recombinantes

Info

Publication number
BR0213259A
BR0213259A BR0213259-1A BR0213259A BR0213259A BR 0213259 A BR0213259 A BR 0213259A BR 0213259 A BR0213259 A BR 0213259A BR 0213259 A BR0213259 A BR 0213259A
Authority
BR
Brazil
Prior art keywords
cells
bioactive
bioactivity
bioactive compound
transformed
Prior art date
Application number
BR0213259-1A
Other languages
English (en)
Inventor
Steven Gary Kendrew
Hrvoje Petkovic
Peter Francis Leadlay
Hamish Alastair Irvin Mcarthur
Original Assignee
Biotica Tech Ltd
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotica Tech Ltd, Pfizer filed Critical Biotica Tech Ltd
Publication of BR0213259A publication Critical patent/BR0213259A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODO PARA PRODUZIR UM COMPOSTO BIOATIVO APRESENTANDO UMA BIOATIVIDADE CONHECIDA, E, CéLULAS RECOMBINANTES". Um composto bioativo, ou família de compostos, é biossintetizado por uma célula utilizando uma pluralidade de atividades enzimáticas. A Requerente proporcionou células hospedeiras que substancialmente não apresentam a bioatividade, mas que apresentam pelo menos uma primeira das atividades enzimáticas (ou ácido nucleico que codifica uma enzima correspondente). As células são transformadas com ácido nucleico expressável para proporcionar pelo menos uma segunda atividade enzimática que permite que as células produzam um composto bioativo. Uma família de células pode ser transformada com uma multiplicidade de ácidos nucleicos diferentes, levando a uma coleção de células incluindo células que produzem produto bioativo diferente, e células que não produzem produtos bioativos. Células transformadas podem ser triadas quanto à bioatividade. Células ativas podem ser isoladas e cultivadas, e compostos bioativos podem ser isolados.
BR0213259-1A 2001-10-17 2002-10-17 Método para produzir um composto bioativo apresentando uma bioatividade conhecida, e, células recombinantes BR0213259A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0125043.0A GB0125043D0 (en) 2001-10-17 2001-10-17 Production, detection and use of transformant cells
PCT/GB2002/004695 WO2003033699A2 (en) 2001-10-17 2002-10-17 Production, detection and use of transformant cells

Publications (1)

Publication Number Publication Date
BR0213259A true BR0213259A (pt) 2004-09-28

Family

ID=9924093

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0213259-1A BR0213259A (pt) 2001-10-17 2002-10-17 Método para produzir um composto bioativo apresentando uma bioatividade conhecida, e, células recombinantes

Country Status (9)

Country Link
US (1) US20060269528A1 (pt)
EP (1) EP1442128A2 (pt)
JP (1) JP2005505301A (pt)
AU (1) AU2002334186A1 (pt)
BR (1) BR0213259A (pt)
CA (1) CA2463167A1 (pt)
GB (1) GB0125043D0 (pt)
MX (1) MXPA04003444A (pt)
WO (1) WO2003033699A2 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0327720D0 (en) * 2003-11-28 2003-12-31 Biotica Tech Ltd Erythromycins and process for their preparation
GB0327721D0 (en) * 2003-11-28 2003-12-31 Biotica Tech Ltd Polyketides and their synthesis
GB0417852D0 (en) 2004-08-11 2004-09-15 Biotica Tech Ltd Production of polyketides and other natural products
JP5421109B2 (ja) 2006-09-13 2014-02-19 ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク 低カルボキシル化／非カルボキシル化オステオカルシンはベータ細胞増殖、インスリン分泌、インスリン感受性、耐糖能を増加させ、体脂肪量を減少させる
US8691553B2 (en) * 2008-01-22 2014-04-08 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
CN103834605B (zh) * 2012-11-21 2018-09-21 中国科学院上海有机化学研究所 一种阿维菌素产生菌及其制备方法和应用
CN105372373A (zh) * 2015-12-10 2016-03-02 宜昌东阳光长江药业股份有限公司 一种克拉霉素的杂质检测方法
CN111778222B (zh) * 2019-04-04 2024-03-22 中国科学院微生物研究所 一个能在真菌中产生黄酮类化合物的nrps-pks杂合蛋白及其编码基因与应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69434408T2 (de) * 1993-09-20 2006-03-16 The Leland Stanford Junior University, Palo Alto Recombinante Herstellung aromatischer Polyketiden
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
US5672491A (en) * 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
KR20040041639A (ko) * 1995-04-24 2004-05-17 크로막솜 코포레이티드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5773221A (en) * 1995-12-15 1998-06-30 Oceanix Biosciences Corporation Method of recovering a biological molecule from a recombinant microorganism
US6242211B1 (en) * 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6033883A (en) * 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
DE60032370T2 (de) * 1999-08-19 2007-10-11 Kirin Beer K.K. Hefevarianten und verfahren zur herstellung von glykoprotein enthaltenden zuckerketten vom säugetiertyp

Also Published As

Publication number Publication date
JP2005505301A (ja) 2005-02-24
US20060269528A1 (en) 2006-11-30
GB0125043D0 (en) 2001-12-12
AU2002334186A1 (en) 2003-04-28
WO2003033699A2 (en) 2003-04-24
MXPA04003444A (es) 2004-07-16
WO2003033699A3 (en) 2003-09-25
EP1442128A2 (en) 2004-08-04
CA2463167A1 (en) 2003-04-24

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A E 7A ANUIDADES

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2060 DE 29/06/2010.