MXPA04003444A - Produccion, deteccion y uso de celulas transformantes. - Google Patents

Produccion, deteccion y uso de celulas transformantes.

Info

Publication number
MXPA04003444A
MXPA04003444A MXPA04003444A MXPA04003444A MXPA04003444A MX PA04003444 A MXPA04003444 A MX PA04003444A MX PA04003444 A MXPA04003444 A MX PA04003444A MX PA04003444 A MXPA04003444 A MX PA04003444A MX PA04003444 A MXPA04003444 A MX PA04003444A
Authority
MX
Mexico
Prior art keywords
cells
bioactive
transformed
bioactivity
family
Prior art date
Application number
MXPA04003444A
Other languages
English (en)
Inventor
Alastair Irvine Mcarthu Hamish
Original Assignee
Biotica Tech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotica Tech Ltd filed Critical Biotica Tech Ltd
Publication of MXPA04003444A publication Critical patent/MXPA04003444A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invencion se refiere a un compuesto biactivo, o familia de compuestos, sometidos a biosintesis por una celula, usando una pluralidad de actividades enzimaticas. Se proporcionan celulas hospedadoras que carecen substancialmente de la bioactividad, pero poseen al menos una primera actividad enzimatica (o acido nucleico que codifica una enzima correspondiente). Las celulas son transformadas con acido nucleico que se puede expresar para proporcionar al menos, una segunda actividad enzimatica la cual habilita a las celulas a las celulas para producir un compuesto bioactivo. Una familia de celulas puede ser transformada con una multiplicidad de diferentes acidos nucleicos, conduciendo a una biblioteca de celulas que incluyen celulas que producen diferentes productos bioactivos, y celulas que no producen compuestos bioactivos. Las celulas transformadas pueden ser seleccionadas por bioactividad. Las celulas activas pueden ser aisladas y cultivadas, y los compuestos bioactivos pueden ser aislados.
MXPA04003444A 2001-10-17 2002-10-17 Produccion, deteccion y uso de celulas transformantes. MXPA04003444A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0125043.0A GB0125043D0 (en) 2001-10-17 2001-10-17 Production, detection and use of transformant cells
PCT/GB2002/004695 WO2003033699A2 (en) 2001-10-17 2002-10-17 Production, detection and use of transformant cells

Publications (1)

Publication Number Publication Date
MXPA04003444A true MXPA04003444A (es) 2004-07-16

Family

ID=9924093

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA04003444A MXPA04003444A (es) 2001-10-17 2002-10-17 Produccion, deteccion y uso de celulas transformantes.

Country Status (9)

Country Link
US (1) US20060269528A1 (es)
EP (1) EP1442128A2 (es)
JP (1) JP2005505301A (es)
AU (1) AU2002334186A1 (es)
BR (1) BR0213259A (es)
CA (1) CA2463167A1 (es)
GB (1) GB0125043D0 (es)
MX (1) MXPA04003444A (es)
WO (1) WO2003033699A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0327720D0 (en) * 2003-11-28 2003-12-31 Biotica Tech Ltd Erythromycins and process for their preparation
GB0327721D0 (en) * 2003-11-28 2003-12-31 Biotica Tech Ltd Polyketides and their synthesis
GB0417852D0 (en) 2004-08-11 2004-09-15 Biotica Tech Ltd Production of polyketides and other natural products
WO2008033518A2 (en) 2006-09-13 2008-03-20 The Trustees Of Columbia University In The City Of New York Undercarboxylated/uncarboxylated osteocalcin increases beta-cell proliferation, insulin secretion, insulin sensitivity, glucose tolerance and decreases fat mass
US7803589B2 (en) * 2008-01-22 2010-09-28 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
CN103834605B (zh) * 2012-11-21 2018-09-21 中国科学院上海有机化学研究所 一种阿维菌素产生菌及其制备方法和应用
CN105372373A (zh) * 2015-12-10 2016-03-02 宜昌东阳光长江药业股份有限公司 一种克拉霉素的杂质检测方法
CN111778222B (zh) * 2019-04-04 2024-03-22 中国科学院微生物研究所 一个能在真菌中产生黄酮类化合物的nrps-pks杂合蛋白及其编码基因与应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
US5672491A (en) * 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
DE69434408T2 (de) * 1993-09-20 2006-03-16 The Leland Stanford Junior University, Palo Alto Recombinante Herstellung aromatischer Polyketiden
CA2219136A1 (en) * 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5773221A (en) * 1995-12-15 1998-06-30 Oceanix Biosciences Corporation Method of recovering a biological molecule from a recombinant microorganism
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6242211B1 (en) * 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US6033883A (en) * 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
EP1211310B1 (en) * 1999-08-19 2006-12-13 Kirin Beer Kabushiki Kaisha Novel yeast variants and process for producing glycoprotein containing mammalian type sugar chain

Also Published As

Publication number Publication date
JP2005505301A (ja) 2005-02-24
GB0125043D0 (en) 2001-12-12
CA2463167A1 (en) 2003-04-24
EP1442128A2 (en) 2004-08-04
WO2003033699A2 (en) 2003-04-24
AU2002334186A1 (en) 2003-04-28
BR0213259A (pt) 2004-09-28
WO2003033699A3 (en) 2003-09-25
US20060269528A1 (en) 2006-11-30

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Legal Events

Date Code Title Description
FA Abandonment or withdrawal