BR0207379A - Uso de proteìnas de fusão cuja parte n-terminal é um derivado de hirudina para produção de proteìnas recombinantes via secreção por levedura - Google Patents

Uso de proteìnas de fusão cuja parte n-terminal é um derivado de hirudina para produção de proteìnas recombinantes via secreção por levedura

Info

Publication number
BR0207379A
BR0207379A BR0207379-0A BR0207379A BR0207379A BR 0207379 A BR0207379 A BR 0207379A BR 0207379 A BR0207379 A BR 0207379A BR 0207379 A BR0207379 A BR 0207379A
Authority
BR
Brazil
Prior art keywords
protein
hirudin
dna sequence
production
amino acid
Prior art date
Application number
BR0207379-0A
Other languages
English (en)
Inventor
Paul Habermann
Original Assignee
Aventis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh
Publication of BR0207379A publication Critical patent/BR0207379A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"USO DE PROTEìNAS DE FUSãO CUJA PARTE N-TERMINAL é UM DERIVADO DE HIRUDINA PARA PRODUçãO DE PROTEìNAS RECOMBINANTES VIA SECREçãO POR LEVEDURA". A presente invenção refere-se a um cassete de expressão da forma P~ x~ - S~ x~ - B~ n~ - (ZR)-Hir(AS~ m~R)-proteína(Y) - T, onde: P~ x~ é qualquer promotor de seq³ência de DNA, selecionado de tal modo que os rendimentos ótimos da proteína de interesse tornam-se adquiríveis, S~ x~ é qualquer DNA codificando uma seq³ência de sinal ou seq³ência líder que permite rendimentos ótimos, B~ n~ São 1-15 códons de aminoácido ou uma ligação química, Z é o códon de um aminoácido selecionado do grupo compreendendo Lys e Arg; R é um códon Arg, ou uma ligação química, As~ m~ é uma ligação química ou códons de aminoácido m, onde m 1-10, Hir é uma seq³ência de DNA codificando para hirudina ou um derivado de hirudina que é pelo menos 40% homólogo à hirudina natural, proteína Y é uma seq³ência de DNA codificando qualquer proteína que pode ser produzida e secretada pela levedura, T é uma seq³ência de DNA não traduzida que é vantajosa para expressão.
BR0207379-0A 2001-02-20 2002-02-08 Uso de proteìnas de fusão cuja parte n-terminal é um derivado de hirudina para produção de proteìnas recombinantes via secreção por levedura BR0207379A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10108211A DE10108211A1 (de) 2001-02-20 2001-02-20 Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
PCT/EP2002/001308 WO2002070722A1 (en) 2001-02-20 2002-02-08 Use of fusion proteins whose n-terminal part is a hirudin derivative for the production of recombinant proteins via secretion by yeasts

Publications (1)

Publication Number Publication Date
BR0207379A true BR0207379A (pt) 2004-06-15

Family

ID=7674912

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0207379-0A BR0207379A (pt) 2001-02-20 2002-02-08 Uso de proteìnas de fusão cuja parte n-terminal é um derivado de hirudina para produção de proteìnas recombinantes via secreção por levedura

Country Status (22)

Country Link
US (1) US20030176673A1 (pt)
EP (1) EP1364032B1 (pt)
JP (1) JP4199543B2 (pt)
KR (1) KR100858833B1 (pt)
CN (1) CN1279171C (pt)
AT (1) ATE342367T1 (pt)
AU (1) AU2002250903B2 (pt)
BR (1) BR0207379A (pt)
CA (1) CA2439042C (pt)
CY (1) CY1106274T1 (pt)
DE (2) DE10108211A1 (pt)
DK (1) DK1364032T3 (pt)
ES (1) ES2272696T3 (pt)
HK (1) HK1065068A1 (pt)
IL (2) IL157416A0 (pt)
MX (1) MXPA03007116A (pt)
NO (1) NO331376B1 (pt)
NZ (1) NZ527642A (pt)
PE (1) PE20020857A1 (pt)
PT (1) PT1364032E (pt)
WO (1) WO2002070722A1 (pt)
ZA (1) ZA200305868B (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7638618B2 (en) * 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE102006031962A1 (de) 2006-07-11 2008-01-17 Sanofi-Aventis Deutschland Gmbh Amidiertes Insulin Glargin
DE102006031955A1 (de) 2006-07-11 2008-01-17 Sanofi-Aventis Deutschland Gmbh Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende
EP2229407B1 (de) 2008-01-09 2016-11-16 Sanofi-Aventis Deutschland GmbH Neue insulinderivate mit extrem verzögertem zeit- / wirkungsprofil
DE102008003566A1 (de) 2008-01-09 2009-07-16 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
EP2229406B1 (de) 2008-01-09 2015-04-22 Sanofi-Aventis Deutschland GmbH Neue insulinderivate mit extrem verzögertem zeit- / wirkungsprofil
DE102008025007A1 (de) 2008-05-24 2009-11-26 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
KR101939557B1 (ko) 2008-10-17 2019-01-17 사노피-아벤티스 도이칠란트 게엠베하 인슐린과 ｇｌｐ－1 효능제의 병용물
CN107308442B (zh) 2009-11-13 2022-10-18 赛诺菲-安万特德国有限公司 包含glp-1激动剂、胰岛素和甲硫氨酸的药物组合物
PT3345593T (pt) 2009-11-13 2023-11-27 Sanofi Aventis Deutschland Composição farmacêutica compreendendo despro36exendina- 4(1-39)-lys6-nh2 e metionina
LT2611458T (lt) 2010-08-30 2016-12-27 Sanofi-Aventis Deutschland Gmbh Ave0010 panaudojimas gaminant vaistą, skirtą 2 tipo cukrinio diabeto gydymui
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
MX370264B (es) 2011-08-29 2019-12-09 Sanofi Aventis Deutschland Combinacion farmaceutica para uso en el control glucemico en pacientes con diabetes de tipo 2.
AR087744A1 (es) 2011-09-01 2014-04-16 Sanofi Aventis Deutschland Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
SI3229828T1 (sl) 2014-12-12 2023-06-30 Sanofi-Aventis Deutschland Gmbh Formulacija s fiksnim razmerjem inzulin glargin/liksisenatid
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341414C (fr) * 1984-03-27 2002-12-31 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
US5705355A (en) * 1984-03-27 1998-01-06 Transgene, S.A. Hirudin, pharmaceutical compositions comprising it and their use
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
US6875589B1 (en) * 1988-06-23 2005-04-05 Hoechst Aktiengesellschaft Mini-proinsulin, its preparation and use
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
ATE176500T1 (de) * 1990-11-08 1999-02-15 Japan Energy Corp Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus
FI92601C (fi) * 1992-03-11 1994-12-12 Marja Makarow Menetelmä hyötyproteiinien erittämiseksi hiivoista
DE19543737A1 (de) * 1995-11-24 1997-05-28 Hoechst Ag Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de) * 1995-11-28 1997-06-05 Hoechst Ag Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
US6150133A (en) * 1996-03-13 2000-11-21 Delta Biotechnology Limited Fermentation control
DE10033195A1 (de) * 2000-07-07 2002-03-21 Aventis Pharma Gmbh Bifunktionale Fusionsproteine aus Hirudin und TAP
US7202059B2 (en) * 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium

Also Published As

Publication number Publication date
JP2004518446A (ja) 2004-06-24
NO20033672L (no) 2003-10-17
IL157416A0 (en) 2004-03-28
AU2002250903B2 (en) 2006-12-07
HK1065068A1 (en) 2005-02-08
CN1279171C (zh) 2006-10-11
CA2439042A1 (en) 2002-09-12
CY1106274T1 (el) 2011-10-12
CA2439042C (en) 2013-06-18
WO2002070722A1 (en) 2002-09-12
DE60215315T2 (de) 2007-05-24
DK1364032T3 (da) 2007-02-19
ATE342367T1 (de) 2006-11-15
IL157416A (en) 2009-08-03
PE20020857A1 (es) 2002-11-04
PT1364032E (pt) 2007-01-31
NZ527642A (en) 2005-03-24
DE60215315D1 (de) 2006-11-23
KR100858833B1 (ko) 2008-09-17
JP4199543B2 (ja) 2008-12-17
MXPA03007116A (es) 2003-11-18
EP1364032A1 (en) 2003-11-26
NO20033672D0 (no) 2003-08-19
ZA200305868B (en) 2005-02-23
KR20030074841A (ko) 2003-09-19
US20030176673A1 (en) 2003-09-18
NO331376B1 (no) 2011-12-12
DE10108211A1 (de) 2002-08-22
ES2272696T3 (es) 2007-05-01
EP1364032B1 (en) 2006-10-11
CN1526021A (zh) 2004-09-01

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Legal Events

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B25D Requested change of name of applicant approved

Owner name: SANOFI-AVENTIS DEUTSCHLAND GMBH (DE)

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 15/81 , C12N 15/62 , C12P 21/02 , C12P 21/06 , C07K 14/815 , C07K 14/62 , C12N 15/17 , C12N 1/19

Ipc: C12N 15/81 (2006.01), C12N 15/62 (2006.01), C12P 2

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8 E 11, 8 E 13, 25 DA LPI

B09B Patent application refused [chapter 9.2 patent gazette]

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