BR0017119A - Isolados bacterianos do gênero klebsiella e um gene de sintase isomaltulose isolado deles - Google Patents

Isolados bacterianos do gênero klebsiella e um gene de sintase isomaltulose isolado deles

Info

Publication number
BR0017119A
BR0017119A BR0017119-0A BR0017119A BR0017119A BR 0017119 A BR0017119 A BR 0017119A BR 0017119 A BR0017119 A BR 0017119A BR 0017119 A BR0017119 A BR 0017119A
Authority
BR
Brazil
Prior art keywords
isomaltulose
kis
clones
synthase gene
plant
Prior art date
Application number
BR0017119-0A
Other languages
English (en)
Inventor
Lian Hui Zhang
Xianzhen Li
Daohai Zhang
Original Assignee
Inst Of Molecular Agrobiology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Of Molecular Agrobiology filed Critical Inst Of Molecular Agrobiology
Publication of BR0017119A publication Critical patent/BR0017119A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99011—Isomaltulose synthase (5.4.99.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/22—Klebsiella

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

"ISOLADOS BACTERIANOS DO GêNERO KLEBSIELLA E UM GENE DE SINTASE ISOMALTULOSE ISOLADO DELES". Duas cepas de novas espécies bacterianas, Klebsiella singaporensis LX3 e LX21, são reivindicadas. Uma seq³ência de nucleotídio (kis) codificando uma nova forma de sintase isomaltulose, KIS, também é reivindicada. Também é reivindicado um método para a produção de isomaltulose em uma planta, no qual o método compreende introduzir em uma célula de tal planta uma seq³ência de ácido nucléico, que codifica uma enzima que converte sacarose em isomaltulose, de uma maneira que permite que a dita célula expresse a dita seq³ência de ácido nucléico, bem como um método de clonagem funcional de isolar a seq³ência de nucleotídio codificando a proteína KIS, compreendendo as etapas de (a) preparar um banco de genes de um organismo doador que contém uma codificação de seq³ência de DNA para uma atividade de biossíntese de isomaltulose em um organismo hospedeiro adequado; (b) selecionar os clones de interesse do banco de genes pelos seus teores de açúcar redutor melhorados; e (c) isolar os clones que contêm uma codificação de DNA para uma proteína com atividade de biossíntese de isomaltulose.
BR0017119-0A 2000-02-15 2000-02-15 Isolados bacterianos do gênero klebsiella e um gene de sintase isomaltulose isolado deles BR0017119A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG2000/000023 WO2001060993A1 (en) 2000-02-15 2000-02-15 Bacterial isolates of the genus klebsiella, and an isomaltulose synthase gene isolated therefrom

Publications (1)

Publication Number Publication Date
BR0017119A true BR0017119A (pt) 2003-01-14

Family

ID=20428789

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0017119-0A BR0017119A (pt) 2000-02-15 2000-02-15 Isolados bacterianos do gênero klebsiella e um gene de sintase isomaltulose isolado deles

Country Status (9)

Country Link
US (1) US7235712B1 (pt)
EP (1) EP1257638B1 (pt)
JP (1) JP2003523203A (pt)
CN (1) CN1434861A (pt)
AT (1) ATE368736T1 (pt)
AU (2) AU2000228416B2 (pt)
BR (1) BR0017119A (pt)
DE (1) DE60035801T2 (pt)
WO (1) WO2001060993A1 (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPQ976800A0 (en) * 2000-08-29 2000-09-21 University Of Queensland, The Novel polypeptides and polynucleotides and uses therefor
AU2003902253A0 (en) * 2003-05-12 2003-05-29 The University Of Queensland Method for increasing product yield
CN101033453B (zh) * 2006-03-07 2010-07-14 中国科学院过程工程研究所 克雷伯氏菌及其在脱除化石燃料中有机氮的应用
AU2009257484B2 (en) 2008-06-11 2015-09-03 Syngenta Participations Ag Compositions and methods for producing fermentable carbohydrates in plants
BR122019001300B1 (pt) 2009-12-23 2020-03-03 Evonik Degussa Gmbh Processo para produção de adoçantes
DE102011100772A1 (de) 2011-05-05 2012-11-08 Evonik Degussa Gmbh Verfahren zur Herstellung von Isomaltulose aus Pflanzensäften
DE102011083030A1 (de) 2011-09-20 2013-03-21 Evonik Degussa Gmbh Mischungszusammensetzung und deren Verwendung als Süßungsmittel
CN102604863B (zh) * 2012-03-01 2013-03-27 中国科学院南海海洋研究所 一种红树林克雷伯氏菌及其在生产1,3-丙二醇中的应用
CN103497944A (zh) * 2013-04-23 2014-01-08 李宪臻 一种蔗糖异构酶的制备方法
DE102014203964A1 (de) 2014-03-05 2015-09-10 Evonik Degussa Gmbh Granulat enthaltend Isomaltulose Synthase
CN104059901A (zh) * 2014-05-06 2014-09-24 江南大学 一种热稳定性和分泌效率提高的蔗糖异构酶突变体及其制备方法
EP3363909A1 (en) 2017-02-15 2018-08-22 Evonik Degussa GmbH Process for production of a solid material containing isomaltulose crystals and trehalulose
EP3653708A1 (en) 2018-11-14 2020-05-20 Evonik Operations GmbH Isomaltulose production
CN114277049A (zh) * 2021-12-31 2022-04-05 李宪臻 一种异源表达蔗糖异构酶的基因工程菌及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4447471C2 (de) 1994-01-19 1995-12-21 Suedzucker Ag Protein mit Palatinase-Aktivität und dafür codierende Nukleinsäure
DE19523560A1 (de) 1995-06-28 1997-01-02 Suedzucker Ag Saccharose-Stoffwechsel-Mutanten
GB9521848D0 (en) * 1995-10-25 1996-01-03 Zeneca Ltd Modification of lignin synthesis in plants

Also Published As

Publication number Publication date
DE60035801D1 (de) 2007-09-13
AU2841600A (en) 2001-08-27
EP1257638A1 (en) 2002-11-20
WO2001060993A1 (en) 2001-08-23
AU2000228416B2 (en) 2006-02-09
CN1434861A (zh) 2003-08-06
US7235712B1 (en) 2007-06-26
JP2003523203A (ja) 2003-08-05
EP1257638B1 (en) 2007-08-01
DE60035801T2 (de) 2008-04-17
ATE368736T1 (de) 2007-08-15

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