BR0010963A - Célula hospedeira recombinante, métodos para enzimaticamente degradar um oligossacarìdeo, para identificar um promotor substituto capaz de aumentar a expressão de um gene-de-interesse em uma célula hospedeira, para produzir uma célula hospedeira recombinante para uso em simultâneas sacarificação e fermentação, para produzir uma célula hospedeira recombinante integrante, para expressar uma polissacarase em uma célula hospedeira e para produzir etanol de uma fonte de oligossacarìdeo, e, vetor - Google Patents

Célula hospedeira recombinante, métodos para enzimaticamente degradar um oligossacarìdeo, para identificar um promotor substituto capaz de aumentar a expressão de um gene-de-interesse em uma célula hospedeira, para produzir uma célula hospedeira recombinante para uso em simultâneas sacarificação e fermentação, para produzir uma célula hospedeira recombinante integrante, para expressar uma polissacarase em uma célula hospedeira e para produzir etanol de uma fonte de oligossacarìdeo, e, vetor

Info

Publication number
BR0010963A
BR0010963A BR0010963-0A BR0010963A BR0010963A BR 0010963 A BR0010963 A BR 0010963A BR 0010963 A BR0010963 A BR 0010963A BR 0010963 A BR0010963 A BR 0010963A
Authority
BR
Brazil
Prior art keywords
host cell
produce
recombinant host
polysaccharase
oligosaccharide
Prior art date
Application number
BR0010963-0A
Other languages
English (en)
Inventor
Lonnie O Ingram
Original Assignee
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida filed Critical Univ Florida
Publication of BR0010963A publication Critical patent/BR0010963A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1051Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/065Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/582Recycling of unreacted starting or intermediate materials

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"CéLULA HOSPEDEIRA RECOMBINANTE, MéTODOS PARA ENZIMATICAMENTE DEGRADAR UM OLIGOSSACARìDEO, PARA IDENTIFICAR UM PROMOTOR SUBSTITUTO CAPAZ DE AUMENTAR A EXPRESSãO DE UM GENE-DE-INTERESSE EM UMA CéLULA HOSPEDEIRA, PARA PRODUZIR UMA CéLULA HOSPEDEIRA RECOMBINANTE PARA USO EM SIMULTâNEAS SACARIFICAçãO E FERMENTAçãO, PARA PRODUZIR UMA CéLULA HOSPEDEIRA RECOMBINANTE INTEGRANTE, PARA EXPRESSAR UMA POLISSACARASE EM UMA CéLULA HOSPEDEIRA E PARA PRODUZIR ETANOL DE UMA FONTE DE OLIGOSSACARìDEO, E, VETOR". A invenção provê células hospedeiras recombinantes, contendo pelo menos um polinucleotídeo heterólogo codificando uma polissacarase sob o controle transcricional de um promotor substituto, capaz de aumentar a expressão da polissacarase. Além disso, a invenção fornece ainda tais hospedeiros com genes codificando proteína(s) secretória(s), para facilitar a secreção da polissacarase expressa. Os hospedeiros preferidos da invenção são etanologênicos e capazes de realizar simultâneas sacarificação e fermentação, resultando na produção de etanol de substratos de celulose complexos.
BR0010963-0A 1999-05-26 2000-05-26 Célula hospedeira recombinante, métodos para enzimaticamente degradar um oligossacarìdeo, para identificar um promotor substituto capaz de aumentar a expressão de um gene-de-interesse em uma célula hospedeira, para produzir uma célula hospedeira recombinante para uso em simultâneas sacarificação e fermentação, para produzir uma célula hospedeira recombinante integrante, para expressar uma polissacarase em uma célula hospedeira e para produzir etanol de uma fonte de oligossacarìdeo, e, vetor BR0010963A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13637699P 1999-05-26 1999-05-26
PCT/US2000/014773 WO2000071729A2 (en) 1999-05-26 2000-05-26 Recombinant hosts suitable for simultaneous saccharification and fermentation

Publications (1)

Publication Number Publication Date
BR0010963A true BR0010963A (pt) 2002-03-19

Family

ID=22472582

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0010963-0A BR0010963A (pt) 1999-05-26 2000-05-26 Célula hospedeira recombinante, métodos para enzimaticamente degradar um oligossacarìdeo, para identificar um promotor substituto capaz de aumentar a expressão de um gene-de-interesse em uma célula hospedeira, para produzir uma célula hospedeira recombinante para uso em simultâneas sacarificação e fermentação, para produzir uma célula hospedeira recombinante integrante, para expressar uma polissacarase em uma célula hospedeira e para produzir etanol de uma fonte de oligossacarìdeo, e, vetor

Country Status (12)

Country Link
US (1) US7226776B2 (pt)
EP (1) EP1185672A2 (pt)
JP (1) JP2003500058A (pt)
KR (2) KR100830032B1 (pt)
CN (2) CN101200701A (pt)
AU (1) AU783260B2 (pt)
BR (1) BR0010963A (pt)
CA (1) CA2374524A1 (pt)
HK (1) HK1045328A1 (pt)
MX (1) MXPA01011991A (pt)
NZ (1) NZ516345A (pt)
WO (1) WO2000071729A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ523192A (en) * 2000-06-26 2005-03-24 Univ Florida Methods and compositions for simultaneous saccharification and fermentation
US7413882B2 (en) 2004-03-25 2008-08-19 Novozymes, Inc. Methods for degrading or converting plant cell wall polysaccharides
JP4756276B2 (ja) * 2006-10-16 2011-08-24 独立行政法人森林総合研究所 エタノールの製造方法
US20100285534A1 (en) * 2007-04-19 2010-11-11 Mascoma Corporation Combined thermochemical pretreatment and refining of lignocellulosic biomass
KR100950113B1 (ko) * 2007-12-17 2010-03-30 건국대학교 산학협력단 섬유소 분해 및 리그닌 분해 활성을 가진 신균주 엔테로박터 루드위기 ku201-3(kacc91341p) 및 그의 배양물을 함유하는 사료용 발효촉진제
WO2011024065A1 (en) * 2009-08-28 2011-03-03 Avesthagent Limited A method of developing stress tolerant plants exhibiting self-glucogenic properties for use in bioethanol production from lignocellulosic biomass
CN107287250A (zh) * 2009-12-23 2017-10-24 丹尼斯科美国公司 提高同步糖化发酵反应效率的方法
WO2011153516A2 (en) 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
WO2012138942A1 (en) 2011-04-05 2012-10-11 Mascoma Corporation Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
CN104797708A (zh) * 2012-08-29 2015-07-22 拉勒曼德匈牙利流动性管理有限责任公司 酵母中用于木质纤维素衍生的寡聚物cbp的酶的表达
MX2016001881A (es) 2013-08-15 2016-08-03 Lallemand Hungary Liquidity Man Llc Metodos para la mejora de rendimiento y produccion de producto en un microorganismo a traves del reciclaje de glicerol.

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5839517B2 (ja) 1974-09-20 1983-08-30 カブシキガイシヤ バイオリサ−チセンタ− セルロ−スカラ アルコ−ルオセイゾウスル ホウホウ
US5162516A (en) * 1988-05-31 1992-11-10 University Of Florida Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis
US5000000A (en) * 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5482846A (en) * 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5821093A (en) * 1988-08-31 1998-10-13 University Of Florida Research Foundation, Inc. Recombinant cells that highly express chromosomally-integrated heterologous genes
US5554520A (en) * 1988-08-31 1996-09-10 Bioenergy International, L.C. Ethanol production by recombinant hosts
US5424202A (en) * 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5028539A (en) * 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5487989A (en) * 1988-08-31 1996-01-30 Bioenergy International, L.C. Ethanol production by recombinant hosts
EP0973882A1 (en) 1997-04-07 2000-01-26 University Of Florida Research Foundation, Inc. DEVELOPMENT OF HIGH-ETHANOL RESISTANT $i(ESCHERICHIA COLI)

Also Published As

Publication number Publication date
CN100335639C (zh) 2007-09-05
AU783260B2 (en) 2005-10-06
WO2000071729A2 (en) 2000-11-30
KR20020033629A (ko) 2002-05-07
CA2374524A1 (en) 2000-11-30
NZ516345A (en) 2004-02-27
WO2000071729A3 (en) 2001-08-30
JP2003500058A (ja) 2003-01-07
WO2000071729A9 (en) 2002-07-04
US20040067555A1 (en) 2004-04-08
HK1045328A1 (zh) 2002-11-22
CN1375008A (zh) 2002-10-16
AU5590600A (en) 2000-12-12
US7226776B2 (en) 2007-06-05
MXPA01011991A (es) 2003-09-04
EP1185672A2 (en) 2002-03-13
CN101200701A (zh) 2008-06-18
KR100830032B1 (ko) 2008-05-15
KR20080029012A (ko) 2008-04-02

Similar Documents

Publication Publication Date Title
ES2536878T3 (es) Mutantes de alfa-amilasa
McKeehan et al. Purification and partial characterization of the aminoacyl transfer ribonucleic acid binding enzyme from rabbit reticulocytes
BR0010963A (pt) Célula hospedeira recombinante, métodos para enzimaticamente degradar um oligossacarìdeo, para identificar um promotor substituto capaz de aumentar a expressão de um gene-de-interesse em uma célula hospedeira, para produzir uma célula hospedeira recombinante para uso em simultâneas sacarificação e fermentação, para produzir uma célula hospedeira recombinante integrante, para expressar uma polissacarase em uma célula hospedeira e para produzir etanol de uma fonte de oligossacarìdeo, e, vetor
MXPA02012324A (es) Metodos y composiciones para la sacarificacion y fermentacion simultaneas.
Roswit et al. Purification and properties of rat uterine procollagenase
NZ335628A (en) Improved expression vectors comprising a nucleic acid construct with tac and gal bacterial promoters
CA2240097A1 (en) Process for the preparation of recombinant proteins in e.coli by high cell density fermentation
DK97784D0 (da) Dna-segment
CA2195799A1 (en) Bacterial production of hydrophobic polypeptides
Furukawa et al. Isolation and identification of glycosaminoglycans associated with purified nuclei from rat liver
WO2000006726A3 (en) Delta-related polypeptides
Wang et al. Secretory overproduction of a raw starch-degrading glucoamylase in Penicillium oxalicum using strong promoter and signal peptide
Wei et al. Recombinant protein production in the filamentous fungus Trichoderma
Luo et al. Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable β-1, 3-1, 4-glucanase
Zhu et al. An engineered pathway for production of terminally sialylated N-glycoproteins in the periplasm of Escherichia coli
Heidrich et al. Autophosphorylation− Inactivation site of Hexokinase 2 in Saccharomyces cerevisiae
Anderson et al. The mitospecific domain of Mrp7 (bL27) supports mitochondrial translation during fermentation and is required for effective adaptation to respiration
CN103352031B (zh) 一种糖基转移酶基因及应用
Toleman et al. Location and characterization of the O-GlcNAcase active site
CA2033069A1 (en) Saf polypeptide, gene, promoter and use for expression in streptomyces
Somashekar et al. Partial purification and properties of a novel chitosanase secreted by Rhodotorula gracilis
Terao et al. Characterization of the proteins of the small subunits of rat liver ribosomes
CN109022397A (zh) 一种降解主产物为新琼二糖的内切型β-琼胶酶及其应用
Guzman et al. Comparison between avian and human prolyl 4‐hydroxylases: Studies on the holomeric enzymes and their constituent subunits
Wallis et al. The effect of pH on glucoamylase production, glycosylation and chemostat evolution of Aspergillus niger

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 22 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.