AU774766B2 - Amplification method for detection of target nucleic acids involving fluorescence energy transfer - Google Patents

Amplification method for detection of target nucleic acids involving fluorescence energy transfer Download PDF

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Publication number
AU774766B2
AU774766B2 AU64558/00A AU6455800A AU774766B2 AU 774766 B2 AU774766 B2 AU 774766B2 AU 64558/00 A AU64558/00 A AU 64558/00A AU 6455800 A AU6455800 A AU 6455800A AU 774766 B2 AU774766 B2 AU 774766B2
Authority
AU
Australia
Prior art keywords
label
probe
sequence
amplification
acceptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU64558/00A
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English (en)
Other versions
AU6455800A (en
Inventor
Martin Alan Lee
Dario Lyall Leslie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
SECR DEFENCE
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SECR DEFENCE, UK Secretary of State for Defence filed Critical SECR DEFENCE
Publication of AU6455800A publication Critical patent/AU6455800A/en
Application granted granted Critical
Publication of AU774766B2 publication Critical patent/AU774766B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Eye Examination Apparatus (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Geophysics And Detection Of Objects (AREA)
AU64558/00A 1999-08-04 2000-08-03 Amplification method for detection of target nucleic acids involving fluorescence energy transfer Ceased AU774766B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9918237.0A GB9918237D0 (en) 1999-08-04 1999-08-04 Detection system
GB9918237 1999-08-04
PCT/GB2000/003016 WO2001011078A1 (en) 1999-08-04 2000-08-03 Amplification method for detection of target nucleic acids involving fluorescence energy transfer

Publications (2)

Publication Number Publication Date
AU6455800A AU6455800A (en) 2001-03-05
AU774766B2 true AU774766B2 (en) 2004-07-08

Family

ID=10858467

Family Applications (1)

Application Number Title Priority Date Filing Date
AU64558/00A Ceased AU774766B2 (en) 1999-08-04 2000-08-03 Amplification method for detection of target nucleic acids involving fluorescence energy transfer

Country Status (10)

Country Link
US (1) US7015018B1 (enExample)
EP (1) EP1198593B1 (enExample)
JP (1) JP4628625B2 (enExample)
AT (1) ATE287969T1 (enExample)
AU (1) AU774766B2 (enExample)
CA (1) CA2381037C (enExample)
DE (1) DE60017750T2 (enExample)
ES (1) ES2245315T3 (enExample)
GB (1) GB9918237D0 (enExample)
WO (1) WO2001011078A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1377683A4 (en) * 2001-04-11 2004-09-08 Us Gov Health & Human Serv MODIFIED RANDOM SPRAYERS FOR PROBE MARKING
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
US20070059690A1 (en) * 2002-05-31 2007-03-15 Amirul Islam "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
US8658366B2 (en) 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
EP2669376B1 (en) 2011-01-26 2017-08-16 Olympus Corporation Method for identifying polymorphism of nucleic acid molecules
JP5856984B2 (ja) * 2011-01-26 2016-02-10 オリンパス株式会社 核酸分子の多型識別方法
CN112266950A (zh) * 2020-10-23 2021-01-26 深圳澳东检验检测科技有限公司 一种探针引物组合及其检测试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999042611A1 (en) * 1998-02-19 1999-08-26 The Secretary Of State For Defence Method for detection of target nucleic acids using pcr

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2082256T3 (es) * 1992-03-23 1996-03-16 Hoffmann La Roche Metodo de deteccion del adn.
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6440675B1 (en) * 1999-09-17 2002-08-27 Whitehead Institute For Biomedical Research Methods for selecting primers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999042611A1 (en) * 1998-02-19 1999-08-26 The Secretary Of State For Defence Method for detection of target nucleic acids using pcr
AU2538399A (en) * 1998-02-19 1999-09-06 Secretary Of State For Defence, The Method for detection of target nucleic acids using pcr

Also Published As

Publication number Publication date
GB9918237D0 (en) 1999-10-06
WO2001011078A8 (en) 2001-06-14
EP1198593B1 (en) 2005-01-26
DE60017750T2 (de) 2005-12-29
DE60017750D1 (de) 2005-03-03
WO2001011078A1 (en) 2001-02-15
CA2381037C (en) 2011-04-12
US7015018B1 (en) 2006-03-21
ES2245315T3 (es) 2006-01-01
CA2381037A1 (en) 2001-02-15
JP2003506068A (ja) 2003-02-18
ATE287969T1 (de) 2005-02-15
AU6455800A (en) 2001-03-05
JP4628625B2 (ja) 2011-02-09
EP1198593A1 (en) 2002-04-24

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