AU2022375767A1 - Off-target blocking sequences to improve target discrimination by polymerase chain reaction - Google Patents
Off-target blocking sequences to improve target discrimination by polymerase chain reaction Download PDFInfo
- Publication number
- AU2022375767A1 AU2022375767A1 AU2022375767A AU2022375767A AU2022375767A1 AU 2022375767 A1 AU2022375767 A1 AU 2022375767A1 AU 2022375767 A AU2022375767 A AU 2022375767A AU 2022375767 A AU2022375767 A AU 2022375767A AU 2022375767 A1 AU2022375767 A1 AU 2022375767A1
- Authority
- AU
- Australia
- Prior art keywords
- pbnj
- sequence
- target
- reference sequence
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163271522P | 2021-10-25 | 2021-10-25 | |
| US63/271,522 | 2021-10-25 | ||
| PCT/US2022/078666 WO2023076908A1 (en) | 2021-10-25 | 2022-10-25 | Off-target blocking sequences to improve target discrimination by polymerase chain reaction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2022375767A1 true AU2022375767A1 (en) | 2024-05-02 |
Family
ID=86158535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2022375767A Pending AU2022375767A1 (en) | 2021-10-25 | 2022-10-25 | Off-target blocking sequences to improve target discrimination by polymerase chain reaction |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230250467A1 (https=) |
| EP (1) | EP4423297A4 (https=) |
| JP (1) | JP2024538973A (https=) |
| AU (1) | AU2022375767A1 (https=) |
| CA (1) | CA3234121A1 (https=) |
| WO (1) | WO2023076908A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20250340934A1 (en) * | 2024-05-06 | 2025-11-06 | Gt Molecular, Inc. | Selective blocking to detect and amplify low abundant template |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7348146B2 (en) * | 2003-10-02 | 2008-03-25 | Epoch Biosciences, Inc. | Single nucleotide polymorphism analysis of highly polymorphic target sequences |
| US20110287424A1 (en) * | 2009-03-27 | 2011-11-24 | Life Technologies Corporation | Methylation-specific competitive allele-specific taqman polymerase chain reaction (cast-pcr) |
| US9404147B2 (en) * | 2011-12-19 | 2016-08-02 | Gen-Probe Incorporated | Closed nucleic acid structures |
| EP2959016A4 (en) * | 2013-02-21 | 2016-10-12 | Toma Biosciences Inc | METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS |
| CN114959098A (zh) * | 2016-07-01 | 2022-08-30 | 嘉士伯有限公司 | 通过应用混合分裂方法筛选生物群体内突变体的方法 |
| WO2022192732A1 (en) * | 2021-03-12 | 2022-09-15 | Gt Molecular, Llc | Multiplexed genotyping assays with a single probe using fluorescent amplitude tuning |
-
2022
- 2022-10-25 WO PCT/US2022/078666 patent/WO2023076908A1/en not_active Ceased
- 2022-10-25 AU AU2022375767A patent/AU2022375767A1/en active Pending
- 2022-10-25 JP JP2024521169A patent/JP2024538973A/ja active Pending
- 2022-10-25 US US18/049,563 patent/US20230250467A1/en active Pending
- 2022-10-25 EP EP22888438.3A patent/EP4423297A4/en active Pending
- 2022-10-25 CA CA3234121A patent/CA3234121A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20230250467A1 (en) | 2023-08-10 |
| CA3234121A1 (en) | 2023-05-04 |
| JP2024538973A (ja) | 2024-10-28 |
| EP4423297A1 (en) | 2024-09-04 |
| WO2023076908A1 (en) | 2023-05-04 |
| EP4423297A4 (en) | 2025-09-24 |
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