AU2022273817B2 - Enzyme variants and uses thereof - Google Patents

Enzyme variants and uses thereof Download PDF

Info

Publication number
AU2022273817B2
AU2022273817B2 AU2022273817A AU2022273817A AU2022273817B2 AU 2022273817 B2 AU2022273817 B2 AU 2022273817B2 AU 2022273817 A AU2022273817 A AU 2022273817A AU 2022273817 A AU2022273817 A AU 2022273817A AU 2022273817 B2 AU2022273817 B2 AU 2022273817B2
Authority
AU
Australia
Prior art keywords
ala
gly
pro
ser
leu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2022273817A
Other languages
English (en)
Other versions
AU2022273817A1 (en
Inventor
Adam Michael Damry
Colin John Jackson
Jake Wesley SAUNDERS
Matthew Spence
Vanessa VONGSOUTHI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Samsara Eco Pty Ltd
Original Assignee
Samsara Eco Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2021901431A external-priority patent/AU2021901431A0/en
Application filed by Samsara Eco Pty Ltd filed Critical Samsara Eco Pty Ltd
Publication of AU2022273817A1 publication Critical patent/AU2022273817A1/en
Application granted granted Critical
Publication of AU2022273817B2 publication Critical patent/AU2022273817B2/en
Priority to AU2023233075A priority Critical patent/AU2023233075A1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00Recovery or working-up of waste materials
    • C08J11/04Recovery or working-up of waste materials of polymers
    • C08J11/10Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44Polycarboxylic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/62Carboxylic acid esters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/50Reuse, recycling or recovery technologies
    • Y02W30/62Plastics recycling; Rubber recycling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2022273817A 2021-05-13 2022-05-13 Enzyme variants and uses thereof Active AU2022273817B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2023233075A AU2023233075A1 (en) 2021-05-13 2023-09-19 Enzyme variants and uses thereof

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2021901431A AU2021901431A0 (en) 2021-05-13 Enzyme variants and uses thereof
AU2021901431 2021-05-13
PCT/AU2022/050455 WO2022236377A1 (en) 2021-05-13 2022-05-13 Enzyme variants and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2023233075A Division AU2023233075A1 (en) 2021-05-13 2023-09-19 Enzyme variants and uses thereof

Publications (2)

Publication Number Publication Date
AU2022273817A1 AU2022273817A1 (en) 2023-04-13
AU2022273817B2 true AU2022273817B2 (en) 2023-06-22

Family

ID=84027760

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2022273817A Active AU2022273817B2 (en) 2021-05-13 2022-05-13 Enzyme variants and uses thereof
AU2023233075A Pending AU2023233075A1 (en) 2021-05-13 2023-09-19 Enzyme variants and uses thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2023233075A Pending AU2023233075A1 (en) 2021-05-13 2023-09-19 Enzyme variants and uses thereof

Country Status (10)

Country Link
US (2) US20240150739A1 (https=)
EP (1) EP4267730A4 (https=)
JP (1) JP2024525117A (https=)
KR (1) KR20240006686A (https=)
CN (1) CN117396601A (https=)
AU (2) AU2022273817B2 (https=)
BR (1) BR112023023499A2 (https=)
CA (1) CA3218464A1 (https=)
MX (1) MX2023013222A (https=)
WO (1) WO2022236377A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4577645A4 (en) * 2022-08-26 2026-01-14 Samsara Eco Pty Ltd VARIANTS OF ENZYMES AND THEIR USES

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402037A (zh) * 2018-11-07 2019-03-01 深伦生物科技(深圳)有限公司 一种用于pet塑料降解的基因工程菌
WO2022182196A1 (ko) * 2021-02-26 2022-09-01 고려대학교 산학협력단 폴리에틸렌테레프탈레이트 재활용을 위한 pet의 화학적 및 생물학적 통합 분해공정

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102261596B1 (ko) * 2020-01-06 2021-06-07 경북대학교 산학협력단 말단을 가지는 폴리에틸렌 테레프탈레이트 고분자에 대한 외부 가수분해 활성을 가지는 MHETase 변이체
AU2022335930A1 (en) * 2021-09-06 2024-03-07 Samsara Eco Pty Limited Recycling of polyester

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402037A (zh) * 2018-11-07 2019-03-01 深伦生物科技(深圳)有限公司 一种用于pet塑料降解的基因工程菌
WO2022182196A1 (ko) * 2021-02-26 2022-09-01 고려대학교 산학협력단 폴리에틸렌테레프탈레이트 재활용을 위한 pet의 화학적 및 생물학적 통합 분해공정

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PALM GOTTFRIED J., REISKY LUKAS, BöTTCHER DOMINIQUE, MüLLER HENRIK, MICHELS EMIL A. P., WALCZAK MIRIAM C., BERNDT LEONA,: "Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate", NATURE COMMUNICATIONS, vol. 10, no. 1, 12 April 2019 (2019-04-12), pages 1 - 10, XP055827642, DOI: 10.1038/s41467-019-09326-3 *

Also Published As

Publication number Publication date
MX2023013222A (es) 2024-04-09
US20250136957A1 (en) 2025-05-01
JP2024525117A (ja) 2024-07-10
AU2023233075A1 (en) 2023-11-09
WO2022236377A1 (en) 2022-11-17
AU2022273817A1 (en) 2023-04-13
EP4267730A1 (en) 2023-11-01
KR20240006686A (ko) 2024-01-15
US20240150739A1 (en) 2024-05-09
EP4267730A4 (en) 2024-07-03
BR112023023499A2 (pt) 2024-02-20
CN117396601A (zh) 2024-01-12
CA3218464A1 (en) 2022-11-17

Similar Documents

Publication Publication Date Title
KR102056250B1 (ko) 재조합 세포, 및 이소프렌의 생산 방법
US12460188B2 (en) Esterases and uses thereof
CN110551752A (zh) xCas9n-epBE碱基编辑系统及其在基因组碱基替换中的应用
US20230399628A1 (en) Novel esterases and uses thereof
AU2022273817B2 (en) Enzyme variants and uses thereof
CN110656122B (zh) 一种提高去甲四环素产量的方法
US9803209B2 (en) Bacterial mutants with improved transformation efficiency
KR101608078B1 (ko) 이산화탄소 유래 숙신산 생산을 위한 균주 및 이를 이용한 이산화탄소 유래 숙신산 생산 방법
CN101040043B (zh) 突变型扩环酶
WO2024040304A1 (en) Enzyme variants and uses thereof
CN113122516B (zh) 一种植物epsps突变体及其在植物中的应用
CN109232726A (zh) 蛋白质OsVPE2在调控植物液泡无机磷输出能力中的应用
CN116814587A (zh) 一种聚酯降解酶突变体及其在聚酯塑料降解中的应用
CN109485707B (zh) 蛋白质OsVPE1在调控植物液泡无机磷输出能力中的应用
CN107674889A (zh) 一种酶反应合成1,2,4‑丁三醇的方法
CN107815435A (zh) 具有增强的纤维素生产能力的葡糖醋杆菌
CN114591996B (zh) 一种凝结芽孢杆菌h-1的表达载体及其构建方法与应用
CN115466319B (zh) 高粱SbMS1蛋白及其编码基因与应用
KR20170129474A (ko) 증가된 셀룰로스 생산능을 갖는 글루콘아세토박터 속 미생물, 그를 이용하여 셀룰로스를 생산하는 방법 및 상기 미생물을 생산하는 방법
CN107988126A (zh) 包含增加丙酮酸磷酸二激酶活性的基因修饰的重组微生物及其用途
KR20180001974A (ko) 증가된 셀룰로스 생산능을 갖는 글루콘아세토박터 속 미생물, 그를 이용하여 셀룰로스를 생산하는 방법 및 상기 미생물을 생산하는 방법

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)