CA3218464A1 - Enzyme variants and uses thereof - Google Patents

Enzyme variants and uses thereof Download PDF

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Publication number
CA3218464A1
CA3218464A1 CA3218464A CA3218464A CA3218464A1 CA 3218464 A1 CA3218464 A1 CA 3218464A1 CA 3218464 A CA3218464 A CA 3218464A CA 3218464 A CA3218464 A CA 3218464A CA 3218464 A1 CA3218464 A1 CA 3218464A1
Authority
CA
Canada
Prior art keywords
amino acid
seq
polypeptide
corresponds
mhetase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3218464A
Other languages
English (en)
French (fr)
Inventor
Jake Wesley SAUNDERS
Matthew Spence
Vanessa VONGSOUTHI
Adam Michael Damry
Colin John Jackson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Samsara Eco Pty Ltd
Original Assignee
Samsara Eco Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2021901431A external-priority patent/AU2021901431A0/en
Application filed by Samsara Eco Pty Ltd filed Critical Samsara Eco Pty Ltd
Publication of CA3218464A1 publication Critical patent/CA3218464A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3218464A 2021-05-13 2022-05-13 Enzyme variants and uses thereof Pending CA3218464A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2021901431A AU2021901431A0 (en) 2021-05-13 Enzyme variants and uses thereof
AU2021901431 2021-05-13
PCT/AU2022/050455 WO2022236377A1 (en) 2021-05-13 2022-05-13 Enzyme variants and uses thereof

Publications (1)

Publication Number Publication Date
CA3218464A1 true CA3218464A1 (en) 2022-11-17

Family

ID=84027760

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3218464A Pending CA3218464A1 (en) 2021-05-13 2022-05-13 Enzyme variants and uses thereof

Country Status (10)

Country Link
US (2) US20240150739A1 (https=)
EP (1) EP4267730A4 (https=)
JP (1) JP2024525117A (https=)
KR (1) KR20240006686A (https=)
CN (1) CN117396601A (https=)
AU (2) AU2022273817B2 (https=)
BR (1) BR112023023499A2 (https=)
CA (1) CA3218464A1 (https=)
MX (1) MX2023013222A (https=)
WO (1) WO2022236377A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4577645A4 (en) * 2022-08-26 2026-01-14 Samsara Eco Pty Ltd VARIANTS OF ENZYMES AND THEIR USES

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402037A (zh) * 2018-11-07 2019-03-01 深伦生物科技（深圳）有限公司 一种用于pet塑料降解的基因工程菌
KR102261596B1 (ko) * 2020-01-06 2021-06-07 경북대학교 산학협력단 말단을 가지는 폴리에틸렌 테레프탈레이트 고분자에 대한 외부 가수분해 활성을 가지는 MHETase 변이체
CN116981725A (zh) * 2021-02-26 2023-10-31 高丽大学校产学协力团 用于回收聚对苯二甲酸乙二醇酯（pet）的pet化学和生物集成降解方法
AU2022335930A1 (en) * 2021-09-06 2024-03-07 Samsara Eco Pty Limited Recycling of polyester

Also Published As

Publication number Publication date
MX2023013222A (es) 2024-04-09
US20250136957A1 (en) 2025-05-01
JP2024525117A (ja) 2024-07-10
AU2023233075A1 (en) 2023-11-09
WO2022236377A1 (en) 2022-11-17
AU2022273817A1 (en) 2023-04-13
EP4267730A1 (en) 2023-11-01
KR20240006686A (ko) 2024-01-15
US20240150739A1 (en) 2024-05-09
EP4267730A4 (en) 2024-07-03
AU2022273817B2 (en) 2023-06-22
BR112023023499A2 (pt) 2024-02-20
CN117396601A (zh) 2024-01-12

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