AU2021106196A4 - Substrate with rice husk and cotton seed husk for pleurotus geesteranus cultivation and method for preparing same - Google Patents
Substrate with rice husk and cotton seed husk for pleurotus geesteranus cultivation and method for preparing same Download PDFInfo
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- 239000010903 husk Substances 0.000 title claims abstract description 67
- 239000000758 substrate Substances 0.000 title claims abstract description 54
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 41
- 235000009566 rice Nutrition 0.000 title claims abstract description 41
- 241000222350 Pleurotus Species 0.000 title claims abstract description 31
- 229920000742 Cotton Polymers 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims description 14
- 240000007594 Oryza sativa Species 0.000 title abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 240000008042 Zea mays Species 0.000 claims abstract description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 12
- 235000005822 corn Nutrition 0.000 claims abstract description 12
- 235000013312 flour Nutrition 0.000 claims abstract description 12
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims abstract description 11
- 235000011941 Tilia x europaea Nutrition 0.000 claims abstract description 11
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 11
- 239000010440 gypsum Substances 0.000 claims abstract description 11
- 239000004571 lime Substances 0.000 claims abstract description 11
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 241000209094 Oryza Species 0.000 claims description 38
- 238000002156 mixing Methods 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 10
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 50
- 238000003306 harvesting Methods 0.000 abstract description 24
- 230000001502 supplementing effect Effects 0.000 abstract description 16
- 241000233866 Fungi Species 0.000 abstract description 10
- 239000000463 material Substances 0.000 abstract description 10
- 238000003825 pressing Methods 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 240000000359 Triticum dicoccon Species 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
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- Mushroom Cultivation (AREA)
Abstract
The present disclosure relates to edible fungus cultivation, and discloses a substrate
for Pleurotus geesteranus cultivation. The substrate includes rice husk, cotton seed
husk, wheat bran, corn flour, lime, and gypsum. This cultivation substrate has the
characteristics of simple preparation, abundant source of materials, fast growth of
mycelium. The new substrate of the present disclosure is rich in nutrient with a
guarantee for the good growth of Pleurotus geesteranu, which also solves the
problem of the shortage of raw materials for the Pleurotus geesteranus cultivation in
southern China.
Wheat bran 16%
Corn flour 5%
Gypsum 1.5%
Lime 2%
Adding Adding
0%~75% 0%~75% SMixing well to get the
Rice husk ivin subtrate 4 - Cotton seed husk
cultivation substrate
Controlling the water
content around 65%
Bagging and sterilizing
Figure 1
Bagging the cultivated - Inoculating - Cultivating mycelium -> Pressing buds
substrate and sterilizing FI
Supplementing water Cultivating mycelium Harvesting the first Fruitingmanagement
crop of mushroom
Harvesting the second
crop of mushroom Cultivating mycelium -> Supplementingwater
Harvesting the third
Supplementing water * Cultivating mycelium crp of m hr Fruiting management crop of mushroom
Fruitingmanage n Harvesting the
fourth
Fruiting management crop of mushroom
Figure 2
1/1
Description
Wheat bran 16% Corn flour 5% Gypsum 1.5% Lime 2%
Adding Adding 0%~75%SMixing well to get the 0%~75% Rice husk ivin subtrate 4 - Cotton seed husk cultivation substrate
Controlling the water content around 65%
Bagging and sterilizing
Figure 1
Bagging the cultivated - Inoculating - Cultivating mycelium -> Pressing buds
FI substrate and sterilizing
Supplementing water Cultivating mycelium Harvesting the first Fruitingmanagement crop of mushroom
Harvesting the second crop of mushroom Cultivating mycelium -> Supplementingwater
Harvesting the third Supplementing water * Cultivating mycelium crp of m hr Fruiting management crop of mushroom
Fruitingmanage n Harvesting the fourth Fruiting management crop of mushroom
Figure 2
1/1
[0001] The present disclosure relates to a substrate with rice husk and cotton seed husk as main materials for Pleurotus geesteranus cultivation and method for
preparing the same.
[0002] Pleurotus geesteranus has a pleasing appearance with a tender and crisp taste, and delicious. Fresh Pleurotus geesteranus is rich in protein and contains 8
essential amino acids necessary for the human body. Cultivation substrate is the
foundation for the production of Pleurotus geesteranus. Large scale production of
Pleurotus geesteranus needs to consume a huge amount of cultivation substrates.
With the vigorous development of the edible fungus industry in recent years, the price
of traditional Pleurotus geesteranus cultivation substrates such as cotton seed husk
and sawdust has been rising year by year in southern China with poor quality and low
output. Therefore, the research and development of large-quantity, easy-to-obtain and
low-cost alternative substrates is the key to further promoting cultivation of Pleurotus
geesteranusin southern China.
[0003] Pleurotus geesteranus is wood-rot fungi, and the main carbon sources used are lignin and cellulose. Rice, as one of the main food crops in China, has the
annual output exceeding 2x108 tons. Rice husk accounts for 20% of the weight of the
rice with the annual output of 4x107 tons. At present, the utilization of rice husk is
mainly as a fuel energy. However, the burning of a large amount of rice husk can
cause severe pollution to the environment, such as increasing the content of PM2.5 in the air, and even causing smog. If rice husk can be used as a cultivation material for edible fungi, it will not only increase an efficient and green utilization of the rice husk, but also provide a large amount of cheap and easy-to-obtain raw materials for the cultivation of edible fungi. This can promote the development of the edible fungus industry, bring us economic benefits, and reduce environmental pollution.
[0004] So far, there has not any literature report on the utilization of rice husk as a raw material for the Pleurotus geesteranus cultivation. The key problem is that there
is no suitable formula of the cultivation substrate and the mature and high-yield
cultivation method.
[0005] The purpose of the present disclosure is to develop a Pleurotus geesteranus cultivation substrate with high yield, low price and simple cultivation
method. The present disclosure provides a new cultivation substrate using cotton seed
husk and rice husk, a method for preparing the same, and a method for cultivating
Pleurotus geesteranus using the cultivation substrate. The method of using this
substrate to cultivate Pleurotusgeesteranus is simple and easy to master, and does not
have any requirements for equipment and site with low pollution and high yield. At
the same time, it is a reasonable utilization way to turn waste into treasure, which can
not only increase the income of mushroom farmers, but also protect the environment.
[0006] The technical solutions of the present disclosure to solve the
aforementioned technical problems are as follows:
[0007] A substrate with rice husk and cotton seed husk as main materials for Pleurotusgeesteranus cultivation, which includes the following components by mass
percentage: rice husk 0%~75%, cotton seed husk 0%~75%, wheat bran 16.5%, corn
flour 5%, lime 2%, gypsum 1.5%.
[0008] A method for preparing the substrate including steps of:
[0009] Step 1. mixing the following components by mass percentage: rice husk
%~75%, cotton seed husk 0%~75%, wheat bran 16.5%, corn flour 5%, lime 2% and
gypsum 1.5%;
[0010] Step 2. mixing the components well to obtain the cultivation substrate and
putting them into a bag, the water content of the cultivation substrate is 60%- 6 5 %,
and the weight of each bag of the cultivation substrate is 400~500 g;
[0011] Step 3. sterilizing with 121°C high pressure steam for 100 min;
[0012] Step 4. cooling down.
[0013] Compared with the prior art, the present invention has the following positive effects:
[0014] (1) Rice husk as the by-product in rice processing with high yield, wide
source, and low price, is the waste recirculation in rice production.
[0015] (2) The investment and the cost is low, and the substrate preparation
method is simple and easy, and it can be dispersed prepared or large-scale production.
[0016] (3) Good product quality, significant economic benefits, and strong market
competitiveness.
[0017] (4) It can solve the problem of lack of Pleurotus geesteranus cultivation
substrate with high price, and increase benefits for mushroom farmers. At the same
time, the ecological environment is protected.
[0018] Figure 1 shows the preparation flow chart of the new cultivation substrate
for Pleurotus geesteranus.
[0019] Figure 2 shows the flow chart of Pleurotus geesteranus cultivation with
the new substrate.
[0020] The present disclosure will be further described below with reference to
the figures and examples, but the content of the present disclosure is not limited to the following examples.
[0021] The present disclosure aims to solve the problems of the traditional cotton
seed husk that cannot be easily obtained locally with the rising price and cost year by
year as the cultivation material for Pleurotus geesteranus in southern China.
Meanwhile, the rich resource of rice husk has not been effectively applied, and
usually only be used as fuel for a long time which resulting in the waste of resources
and pollution to the ecological environment. The successful utilization of rice husk as
the main material for the Pleurotusgeesteranus cultivation makes it possible to take
advantages of rice husk to lower the cost of Pleurotus geesteranus cultivation and
provides a new way for the recycling and reuse of crop waste. Repeated experiments
have proved that rice husk can be used as the main material for the Pleurotus
geesteranus cultivation. The effect of different mass ratios of rice husk and cotton
seed husk in cultivating Pleurotusgeesteranus is listed in Table 1.
[0022] Table 1 effect of Pleurotus geesteranus cultivation with different mass
ratios of rice husk and cotton seed husk Cotton seed Rice Wheat Corn Gypsu Lime Biological husk % husk % bran % flour % m% % efficiency
% 75 0 16.5 5 1.5 2 74.98 67.5 7.5 16.5 5 1.5 2 47.76 60 15 16.5 5 1.5 2 54.59 52.5 22.5 16.5 5 1.5 2 51.64 45 30 16.5 5 1.5 2 50.96 37.5 37.5 16.5 5 1.5 2 55.59 30 45 16.5 5 1.5 2 43.29 22.5 52.5 16.5 5 1.5 2 55.87 15 60 16.5 5 1.5 2 37.86 7.5 67.5 16.5 5 1.5 2 33.82 0 75 16.5 5 1.5 2 30.25
[0023] Example 1
[0024] A new method for cultivating Pleurotus geesteranus with rice husk and
cotton seed husk as substrate includes the following steps:
[0025] Step 1. mixing the following components by mass percentage: 15% of rice
husk, 60% of cotton seed husk, 1 6 .5 % of wheat bran, 5% of corn flour, 2% of lime
and 1.5% of gypsum;
[0026] Step 2. mixing the components well to obtain the cultivation substrate and
putting them into a bag, the water content of the cultivation substrate is 60%- 6 5 %,
and the weight of each bag of the cultivation substrate is 400~500 g;
[0027] Step 3. sterilizing with 121°C high pressure steam for 100 min;
[0028] Step 4. cooling down and inoculating: cooling the fungus bag to room
temperature and inoculating, the inoculation amount is 20-30 mycelium balls per bag
of cultivation substrate;
[0029] Step 5. cultivating mycelium: cultivating the mycelium for 20-30 days
under the condition of temperature 25°C~30°C and air humidity 30%-50%;
[0030] Step 6. pressing bud and opening the bag, and using the temperature
difference to stimulate it in dark environment under the condition of air humidity
%~80%;
[0031] Step 7. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0032] Step 8. harvesting the first crop of mushroom;
[0033] Step 9. cultivating the mycelium in a dark environment for 3~4 days under
the condition of temperature 25°C~30°C and air humidity 30%~50%;
[0034] Step 10. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70480%;
[0035] Step 11. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0036] Step 12. harvesting the second crop of mushroom;
[0037] Step 13. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%-50%;
[0038] Step 14. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[0039] Step 15. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0040] Step 16. harvesting the third crop of mushroom;
[0041] Step 17. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%~50%;
[0042] Step 18. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[0043] Step 19. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0044] Step 20. harvesting the fourth crop of mushroom;
[0045] Table 2 effect of Pleurotus geesteranus cultivation with 15% rice husk and
60% cotton seed husk
Index Average daily Mushroom wet weight wet wet wet wet Conversion growth of growing of the first weight of weight of weight of weight of rates
% mycelium (cm) cycle(d) crop of the second the third the fourth the four mushroom crop of crop of crop of crops of (g) mushroom mushroom mushroom mushroom zcessin (g) (g) (g) (g) 1 0.257143 31 49.33 32.00 26.12 23.27 130.72 52.28 2 0.357143 41 45.91 38.50 16.00 17.84 118.25 47.30 3 1.071429 23 54.59 41.50 35.08 27.15 158.32 63.32 4 1.014286 36 25.10 35.86 35.51 29.23 125.70 50.28 5 0.357143 33 55.35 32.94 36.94 24.16 149.39 59.75
[0046] Example 2
[0047] A new method for cultivating Pleurotus geesteranus with rice husk and
cotton seed husk as substrate includes the following steps:
[0048] Step 1. mixing the following components by mass percentage: 22.5% of
rice husk, 52.5% of cotton seed husk, 16.5% of wheat bran, 5% of corn flour, 2% of lime and 1.5% of gypsum;
[0049] Step 2. mixing the components well to obtain the cultivation substrate and
putting them into a bag, the water content of the cultivation substrate is 60%-65%,
and the weight of each bag of the cultivation substrate is 400~500g;
[0050] Step 3. sterilizing with 121°C high pressure steam for 100 min;
[0051] Step 4. cooling down and inoculating: cooling the fungus bag to room
temperature and inoculating, the inoculation amount is 20-30 mycelium balls per bag
of cultivation substrate;
[0052] Step 5. cultivating mycelium: cultivating the mycelium for 20-30 days in
dark environment under the condition of temperature 25°C~30°C and air humidity
%~50%;
[0053] Step 6. pressing bud and opening the bag, and using the temperature
difference to stimulate it under the condition of air humidity 70%~80%;
[0054] Step 7. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0055] Step 8. harvesting the first crop of mushroom;
[0056] Step 9. cultivating the mycelium in a dark environment for 3~4 days under
the condition of temperature 25°C~30°C and air humidity 30%~50%;
[0057] Step 10. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[0058] Step 11. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0059] Step 12. harvesting the second crop of mushroom;
[0060] Step 13. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%~50%;
[0061] Step 14. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[0062] Step 15. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0063] Step 16. harvesting the third crop of mushroom;
[0064] Step 17. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%~50%;
[0065] Step 18. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[0066] Step 19. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0067] Step 20. harvesting the fourth crop of mushroom;
[0068] Table 3 effect of Pleurotus geesteranus cultivation with 22.5% rice husk
and 52.5% cotton seed husk as main materials
Index Average Mushroom wet wet wet wet wet Conversion daily growing weight of weight of weight of weight of weight of rates
% growth of cycle(d) the first the second the third the fourth the four hyphae crop of crop of crop of crop of crops of (cm) mushroom mushroom mushroom mushroom mushroom Processin (g) (g) (g) (g) (g) 1 1.071429 30 74.75 17.25 33.96 24.97 150.93 60.37 2 0.242857 42 62.74 14.22 38.42 37.86 153.06 61.22 3 0.514286 30 24.02 44.90 41.89 19.24 130.05 52.02 4 1.142857 28 38.00 34.01 25.11 36.57 133.69 53.47 5 1.071429 42 26.34 28.95 30.40 20.56 106.25 42.50
[0069] Example 3
[0070] A new method for cultivating Pleurotus geesteranus with rice husk and
cotton seed husk as substrate includes the following steps:
[0071] Step 1. mixing the following components by mass percentage: 37.5% of
rice husk, 37.5% of cotton seed husk, 16.5% of wheat bran, 5% of corn flour, 2% of
lime and 1.5% of gypsum;
[0072] Step 2. mixing the components well to obtain the cultivation substrate and
putting them into a bag, the water content of the cultivation substrate is 60%- 6 5 %,
and the weight of each bag of the cultivation substrate is 400~500 g;
[0073] Step 3. sterilizing with 121°C high pressure steam for 100 min;
[0074] Step 4. cooling down and inoculating: cooling the fungus bag to room
temperature and inoculating, the inoculation amount is 20-30 mycelium balls per bag
of cultivation substrate;
[0075] Step 5. cultivating mycelium: cultivating the mycelium for 20-30 days in
dark environment under the condition of temperature 25°C~30°C and air humidity
%~50%;
[0076] Step 6. pressing bud and opening the bag, and using the temperature
difference to stimulate it under the condition of air humidity 70%~80%;
[0077] Step 7. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0078] Step 8. harvesting the first crop of mushroom;
[0079] Step 9. cultivating the mycelium in a dark environment for 3~4 days under
the condition of temperature 25°C~30°C and air humidity 30%~50%;
[0080] Step 10. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70480%;
[0081] Step 11. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0082] Step 12. harvesting the second crop of mushroom;
[0083] Step 13. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%-50%;
[0084] Step 14. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70480%;
[0085] Step 15. fruiting management, cultivating for 2~3 days under the condition of temperature 15°C~25°C and air humidity 85%~90%;
[00861 Step 16. harvesting the third crop of mushroom;
[0087] Step 17. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%~50%;
[0088] Step 18. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[0089] Step 19. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[0090] Step 20. harvesting the fourth crop of mushroom;
[0091] Table 4. effect of Pleurotusgeesteranus cultivation with 37.5% rice husk
and 37.5% cotton seed husk as main materials
Index Average Mushroom wet wet wet wet wet Conversion daily growing weight of weight of weight of weight of weight of rates
% growth of cycle (d) the first the second the third the fourth the four hyphae crop of crop of crop of crop of crops of (cm) mushroom mushroom mushroom mushroom mushroom Processin (g) (g) (g) (g) (g) 1 0.214286 31 60.83 15.35 25.68 30.55 132.41 52.96 2 0.3 30 70.42 37.42 34.00 16.89 158.73 63.49 3 0.457143 28 66.30 26.00 25.36 15.21 132.87 53.14 4 0.342857 31 79.83 28.92 21.74 8.15 138.74 55.49 0.371429 33 68.45 27.00 27.04 9.68 132.17 52.86
[0092] Example 4
[0093] A new method for cultivating Pleurotus geesteranus with rice husk and
cotton seed husk as substrate includes the following steps:
[0094] Step 1. mixing the following components by mass percentage: 52.5% of
rice husk, 22.5% of cotton seed husk, 16.5% of wheat bran, 5% of corn flour, 2% of
lime and 1.5% of gypsum;
[0095] Step 2. mixing the components well to obtain the cultivation substrate and putting them into a bag, the water content of the cultivation substrate is 60%- 6 5 %, and the weight of each bag of the cultivation substrate is 400~500g;
[0096] Step 3. sterilizing with 121°C high pressure steam for 100 min;
[0097] Step 4. cooling down and inoculating: cooling the fungus bag to room
temperature and inoculating, the inoculation amount is 20-30 mycelium balls per bag
of cultivation substrate;
[0098] Step 5. cultivating mycelium: cultivating the mycelium for 20-30 days in
dark environment under the condition of temperature 25°C~30°C and air humidity
%~50%;
[0099] Step 6. pressing bud and opening the bag, and using the temperature
difference to stimulate it under the condition of air humidity 70%~80%;
[00100] Step 7. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[00101] Step 8. harvesting the first crop of mushroom;
[00102] Step 9. cultivating the mycelium in a dark environment for 3~4 days under
the condition of temperature 25°C~30°C and air humidity 30%~50%;
[00103] Step 10. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[00104] Step 11. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[00105] Step 12. harvesting the second crop of mushroom;
[00106] Step 13. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%-50%;
[00107] Step 14. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70480%;
[00108] Step 15. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[00109] Step 16. harvesting the third crop of mushroom;
[00110] Step 17. cultivating the mycelium in a dark environment for 3~4 days
under the condition of temperature 25°C~30°C and air humidity 30%~50%;
[00111] Step 18. supplementing water, cultivating for 3~5 days under a large
temperature difference and the air humidity of 70~80%;
[00112] Step 19. fruiting management, cultivating for 2~3 days under the condition
of temperature 15°C~25°C and air humidity 85%~90%;
[00113] Step 20. harvesting the fourth crop of mushroom;
[00114] Table 5 effect of Pleurotus geesteranus cultivation with 52.5% rice husk
and 22.5% cotton seed husk as main materials
Index Average Mushroom wet wet wet wet wet Conversion daily growing weight of weight of weight of weight of weight of rates
% growth of cycle(d) the first the second the third the fourth the four hyphae crop of crop of crop of crop of crops of (cm) mushroom mushroom mushroom mushroom mushroom Processin (g) (g) (g) (g) (g) 1 1.071429 28 39.37 61.00 21.88 9.21 131.46 52.58 2 0.428571 32 74.32 19.58 29.54 10.92 134.36 53.74 3 0.4 38 68.28 35.28 31.26 11.62 146.44 58.57 4 0.371429 25 80.38 40.00 30.88 11.00 162.26 64.90 5 0.371429 30 56.00 34.18 18.46 15.22 123.86 49.54
Claims (2)
- Claims 1. A substrate with rice husk and cotton seed husk for Pleurotus geesteranuscultivation comprising following components by mass percentage: rice husk 0%-75%,cotton seed husk 0%-75%, wheat bran 16.5%, corn flour 5%, lime 2%, gypsum 1.5%.
- 2. A method for preparing a substrate comprising steps of:step 1. mixing the following components by mass percentage: rice husk 0%~75%,cotton seed husk 0%~75%, wheat bran 16.5%, corn flour 5%, lime2% and gypsum1.5%;step 2. mixing the components well to obtain the cultivation substrate and puttingthem into a bag, the water content of the cultivation substrate is 60%-65%, and theweight of each bag of the cultivation substrate is 400~500g;step 3. sterilizing with 121°C high pressure steam for 100 min;step 4. cooling down.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2021109043214 | 2021-08-06 | ||
| CN202110904321 | 2021-08-06 |
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