AU2019369192B2 - Methods and compositions - Google Patents

Methods and compositions

Info

Publication number
AU2019369192B2
AU2019369192B2 AU2019369192A AU2019369192A AU2019369192B2 AU 2019369192 B2 AU2019369192 B2 AU 2019369192B2 AU 2019369192 A AU2019369192 A AU 2019369192A AU 2019369192 A AU2019369192 A AU 2019369192A AU 2019369192 B2 AU2019369192 B2 AU 2019369192B2
Authority
AU
Australia
Prior art keywords
amino acid
unnatural amino
polypeptide
dap
suitably
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2019369192A
Other languages
English (en)
Other versions
AU2019369192A1 (en
Inventor
Jason Chin
Nicolas HUGUENIN-DEZOT
Mohan MAHESH
Duy P. Nguyen
Shan Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
United Kingdom Research and Innovation
Original Assignee
United Kingdom Research and Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by United Kingdom Research and Innovation filed Critical United Kingdom Research and Innovation
Publication of AU2019369192A1 publication Critical patent/AU2019369192A1/en
Priority to AU2025205622A priority Critical patent/AU2025205622A1/en
Application granted granted Critical
Publication of AU2019369192B2 publication Critical patent/AU2019369192B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D317/00—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms
    • C07D317/08—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3
    • C07D317/44—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3 ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D317/46—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3 ortho- or peri-condensed with carbocyclic rings or ring systems condensed with one six-membered ring
    • C07D317/48—Methylenedioxybenzenes or hydrogenated methylenedioxybenzenes, unsubstituted on the hetero ring
    • C07D317/62—Methylenedioxybenzenes or hydrogenated methylenedioxybenzenes, unsubstituted on the hetero ring with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to atoms of the carbocyclic ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K11/00—Depsipeptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K11/02—Depsipeptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof cyclic, e.g. valinomycins ; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22069—SARS coronavirus main proteinase (3.4.22.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2019369192A 2018-10-26 2019-10-24 Methods and compositions Active AU2019369192B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2025205622A AU2025205622A1 (en) 2018-10-26 2025-07-18 Methods and Compositions

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1817444.1 2018-10-26
GBGB1817444.1A GB201817444D0 (en) 2018-10-26 2018-10-26 Methods and compositions
PCT/GB2019/053023 WO2020084307A1 (en) 2018-10-26 2019-10-24 Methods and compositions

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2025205622A Division AU2025205622A1 (en) 2018-10-26 2025-07-18 Methods and Compositions

Publications (2)

Publication Number Publication Date
AU2019369192A1 AU2019369192A1 (en) 2021-05-13
AU2019369192B2 true AU2019369192B2 (en) 2025-08-14

Family

ID=64560429

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2019369192A Active AU2019369192B2 (en) 2018-10-26 2019-10-24 Methods and compositions
AU2025205622A Pending AU2025205622A1 (en) 2018-10-26 2025-07-18 Methods and Compositions

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2025205622A Pending AU2025205622A1 (en) 2018-10-26 2025-07-18 Methods and Compositions

Country Status (8)

Country Link
US (2) US12286657B2 (https=)
EP (1) EP3870224A1 (https=)
JP (2) JP7633156B2 (https=)
CN (2) CN120607506A (https=)
AU (2) AU2019369192B2 (https=)
CA (1) CA3116567A1 (https=)
GB (1) GB201817444D0 (https=)
WO (1) WO2020084307A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2014DN09922A (https=) 2012-05-18 2015-08-14 Medical Res Council
GB201817444D0 (en) 2018-10-26 2018-12-12 Res & Innovation Uk Methods and compositions
GB201906775D0 (en) 2019-05-14 2019-06-26 Res & Innovation Uk Synthetic genome
CN114195665A (zh) * 2021-12-14 2022-03-18 武汉世吉药业有限公司 一种高纯度ncaa的中和酸化工艺及设备
EP4328308A1 (en) * 2022-08-22 2024-02-28 Technische Universität Berlin Modified trna-synthetase for the incorporation of non-canonical amino acids

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014036492A1 (en) * 2012-08-31 2014-03-06 Sutro Biopharma, Inc. Modified amino acids comprising an azido group

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5275566B2 (ja) 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
AU2004321117B2 (en) 2003-12-18 2010-09-02 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
CA2580840A1 (en) 2004-09-21 2006-03-30 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
EP1807515A4 (en) 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US8431558B2 (en) 2004-11-01 2013-04-30 The Regents Of The University Of California Compositions and methods for modification of biomolecules
JP2007037445A (ja) 2005-08-02 2007-02-15 Institute Of Physical & Chemical Research ｔＲＮＡ合成方法、核酸、アミノアシルｔＲＮＡ合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法
US20090081669A1 (en) 2006-02-01 2009-03-26 Encode Bio, Inc. Fluorescent Assays Using Orthogonal tRNA - Aminoacyl Synthetase Pairs
US20080096819A1 (en) 2006-05-02 2008-04-24 Allozyne, Inc. Amino acid substituted molecules
US20090110662A1 (en) 2007-04-30 2009-04-30 Intezyne Technologies, Inc. Modification of biological targeting groups for the treatment of cancer
JP5590649B2 (ja) 2007-09-20 2014-09-17 独立行政法人理化学研究所 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法
GB0721291D0 (en) 2007-10-30 2007-12-12 Medical Res Council Methods and compositions
GB2470770A (en) 2009-06-04 2010-12-08 Medical Res Council Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs
GB0917242D0 (en) 2009-10-01 2009-11-18 Medical Res Council Acetyl lysine incorporation with tRNA synthetase
GB0917240D0 (en) 2009-10-01 2009-11-18 Medical Res Council Incorporation of methyl lysine into poiypeptide
US8697688B2 (en) 2010-04-15 2014-04-15 Seattle Genetics Inc. Pyrrolobenzodiazepines used to treat proliferative diseases
DK2563753T6 (en) 2010-04-27 2016-04-04 Synaffix Bv Fused cyclooctynforbindelser and their use in metal-free click-reactions
US20120077186A1 (en) 2010-05-07 2012-03-29 Oregon Health & Science University Use of cysteine-derived suppressor trnas for non-native amino acid incorporation
WO2011156686A2 (en) 2010-06-11 2011-12-15 The Regents Of The University Of Colorado, A Body Corporate Method for synthesizing a cyclic multivalent peptide using a thiol-mediated reaction
DK2670767T3 (en) 2011-02-03 2018-03-26 European Molecular Biology Laboratory Non-naturally occurring amino acids comprising a cyclooctynyl or transcyclooctynyl analog group and uses thereof
GB201110815D0 (en) 2011-06-24 2011-08-10 Medical Res Council Incorporation of subtituted lysines into polypeptides
WO2013036748A1 (en) 2011-09-09 2013-03-14 Berry, Lana, L. Bicyclo[6.1.0]non-4-yne reagents for chemical modification of oligonucleotides
GB201201100D0 (en) 2012-01-20 2012-03-07 Medical Res Council Polypeptides and methods
IN2014DN09922A (https=) 2012-05-18 2015-08-14 Medical Res Council
SG10201702387YA (en) 2012-09-24 2017-04-27 Medimmune Ltd Cell lines
EP3087088B1 (en) * 2013-12-23 2022-05-25 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
GB2528227A (en) 2014-03-14 2016-01-20 Medical Res Council Cyclopropene amino acids and methods
GB201419109D0 (en) 2014-10-27 2014-12-10 Medical Res Council Incorporation of unnatural amino acids into proteins
GB201613135D0 (en) 2016-07-29 2016-09-14 Medical Res Council Genome editing
WO2019028419A1 (en) * 2017-08-03 2019-02-07 Synthorx, Inc. CYTOKINE CONJUGATES FOR THE TREATMENT OF PROLIFERATIVE AND INFECTIOUS DISEASES
GB201817444D0 (en) 2018-10-26 2018-12-12 Res & Innovation Uk Methods and compositions
GB201906775D0 (en) 2019-05-14 2019-06-26 Res & Innovation Uk Synthetic genome
CN117881779A (zh) 2021-05-28 2024-04-12 英国研究与创新署 微生物及其用途

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014036492A1 (en) * 2012-08-31 2014-03-06 Sutro Biopharma, Inc. Modified amino acids comprising an azido group

Also Published As

Publication number Publication date
WO2020084307A1 (en) 2020-04-30
AU2025205622A1 (en) 2025-08-07
CA3116567A1 (en) 2020-04-30
US20220010296A1 (en) 2022-01-13
CN113226375A (zh) 2021-08-06
CN120607506A (zh) 2025-09-09
JP2025023942A (ja) 2025-02-19
AU2019369192A1 (en) 2021-05-13
EP3870224A1 (en) 2021-09-01
JP2022505464A (ja) 2022-01-14
US20250283066A1 (en) 2025-09-11
US12286657B2 (en) 2025-04-29
CN113226375B (zh) 2025-05-13
JP7633156B2 (ja) 2025-02-19
GB201817444D0 (en) 2018-12-12

Similar Documents

Publication Publication Date Title
AU2019369192B2 (en) Methods and compositions
US20250340916A1 (en) Method for synthesizing peptides in cell-free translation system
Wexselblatt et al. Design, synthesis and structure–activity relationship of novel Relacin analogs as inhibitors of Rel proteins
JP7073250B2 (ja) チオール基をアミノ基近傍に有するアミノ酸をｎ末端に持つ非環状ペプチド－核酸複合体、そのライブラリー、およびそれから誘導される環状ペプチド－核酸複合体ライブラリーの製造方法
JP5807025B2 (ja) 蛍光プローブ
Basak et al. Structure–kinetic relationship studies for the development of long residence time LpxC inhibitors
US9376700B2 (en) Methods of modifying N-termini of a peptide or protein using transferases
US20240409524A1 (en) Compound having photocleavable moiety, conjugate, and screening method using same
US10067136B1 (en) Photoactivatable probes and uses thereof
KR20250051803A (ko) 비모이어티 의존적 티오에테르 설치 도구로서의 papb
Guesmi et al. Mechanochemical Promoted Heterocycles: A Solvent-free Route to Triazole Carbohydrates as Glycogen Phosphorylase Inhibitors
Hulce et al. An in vivo active carbamate-based dual inhibitor of lysophospholipase 1 (LYPLA1) and lysophospholipase 2 (LYPLA2)
Bag et al. Intramolecular cyclization of isothiocyanyl amino acids/peptide: arrival at unnatural thioxoimidazolidinyl/thioxooxazolidinyl amino acids
Hann Chemical Probes to Trap Ubiquitin Conjugation Cascade Intermediates
Sangsuwan et al. Diethylphosphonate-containing aminoacyl-adenosine analog as inhibitor of bacterial tRNA-dependent transamidase
Albrecht Elucidation of the Diadenosine Triphosphate Interaction Network by Chemical Proteomics
Haas Towards new tools in chemical biology-investigations on phosphate chemistry of magic spot nucleotides and aryne intermediates
Schramma et al. Investigations into the Mechanism and Substrate Scope of AgaB and the Chemo-Enzymatic Total Synthesis of Streptide Natural Products
Groff Photocontrol of biological activity
Wever Studies towards the chemoenzymatic syntheses of thiopeptides and thiomarinol and visible light catalyzed O-glycosylation of thioglycosides
Pham Novel inhibitors of the tRNA-dependent amidotransferase of" Helicobacter pylori": Peptides generated by phage display and dipeptide-like compounds
JP2001048896A (ja) 新規ジアデノシンテトラリン酸およびその製造方法

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)