AU2016218945B2 - EpCAM aptamers and conjugates thereof - Google Patents

EpCAM aptamers and conjugates thereof Download PDF

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Publication number
AU2016218945B2
AU2016218945B2 AU2016218945A AU2016218945A AU2016218945B2 AU 2016218945 B2 AU2016218945 B2 AU 2016218945B2 AU 2016218945 A AU2016218945 A AU 2016218945A AU 2016218945 A AU2016218945 A AU 2016218945A AU 2016218945 B2 AU2016218945 B2 AU 2016218945B2
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Prior art keywords
aptamer
epcam
cells
brain
aptamer conjugate
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AU2016218945A
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AU2016218945A1 (en
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Sarah SHIGDAR
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Deakin University
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Deakin University
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Priority claimed from AU2015900437A external-priority patent/AU2015900437A0/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/51—Physical structure in polymeric form, e.g. multimers, concatemers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2016218945A 2015-02-11 2016-02-11 EpCAM aptamers and conjugates thereof Active AU2016218945B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2015900437A AU2015900437A0 (en) 2015-02-11 Aptamer conjugate
AU2015900437 2015-02-11
PCT/AU2016/050085 WO2016127216A1 (en) 2015-02-11 2016-02-11 Epcam aptamers and conjugates thereof

Publications (2)

Publication Number Publication Date
AU2016218945A1 AU2016218945A1 (en) 2017-08-03
AU2016218945B2 true AU2016218945B2 (en) 2021-08-26

Family

ID=56613973

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016218945A Active AU2016218945B2 (en) 2015-02-11 2016-02-11 EpCAM aptamers and conjugates thereof

Country Status (7)

Country Link
US (1) US10577610B2 (enExample)
EP (1) EP3256593B1 (enExample)
JP (1) JP6978317B2 (enExample)
AU (1) AU2016218945B2 (enExample)
CA (1) CA2975190C (enExample)
ES (1) ES2796504T3 (enExample)
WO (1) WO2016127216A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3185910A4 (en) * 2014-08-29 2018-01-10 Children's Medical Center Corporation Methods and compositions for the treatment of cancer
JP6661645B2 (ja) 2014-10-15 2020-03-11 シティ・オブ・ホープＣｉｔｙ ｏｆ Ｈｏｐｅ トランスフェリン受容体（ｔｆｒ）に対するｒｎａアプタマー
US11219635B2 (en) * 2016-02-19 2022-01-11 City Of Hope Bi-specific aptamer
US11332747B2 (en) * 2017-02-24 2022-05-17 Augusta University Research Institute, Inc. Bispecific aptamer for treating cancer
EP3664814A4 (en) * 2017-08-11 2021-05-05 City of Hope Rna aptamers against transferrin receptor (tfr)
CN112955554A (zh) * 2018-07-26 2021-06-11 奥拉廷加Tnp生物 基于适体的car t细胞开关
MX2021001006A (es) * 2018-07-26 2021-07-15 Aratinga Bio Tnp Interruptor de celulas t-car basado en aptamero.
KR102294694B1 (ko) * 2019-07-18 2021-08-30 국립암센터 신규한 dna 앱타머 및 이의 용도
CN110592093B (zh) * 2019-09-10 2023-08-25 上海交通大学医学院附属仁济医院 一种能够识别EpCAM蛋白的核酸适体及其制备方法与应用
CN112843261B (zh) * 2021-03-10 2022-11-01 中国人民解放军空军军医大学 靶向EpCAM的放射性配合物及其制备方法
JP7359457B2 (ja) * 2021-10-11 2023-10-11 タグシクス・バイオ株式会社 自己免疫性疾患治療薬

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103387988A (zh) * 2012-09-24 2013-11-13 厦门大学 上皮细胞粘附分子的核酸适体EpCAM Ccut及其制备方法
WO2014068408A2 (en) * 2012-10-23 2014-05-08 Caris Life Sciences Switzerland Holdings, S.A.R.L. Aptamers and uses thereof
WO2014197455A1 (en) * 2013-06-03 2014-12-11 University Of Florida Research Foundation, Incorporated Devices and methods for isolating cells

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070178451A1 (en) * 2001-08-02 2007-08-02 Molian Deng Nucleic acid sequences from Chlorella sarokiniana and uses thereof
MX2007002772A (es) 2004-09-07 2008-03-05 Archemix Corp Aptameros para el factor de von willebrand y su uso como agentes terapeuticos para la enfermedad trombotica.
US20110052697A1 (en) 2006-05-17 2011-03-03 Gwangju Institute Of Science & Technology Aptamer-Directed Drug Delivery
EP2159286A1 (en) 2008-09-01 2010-03-03 Consiglio Nazionale Delle Ricerche Method for obtaining oligonucleotide aptamers and uses thereof
WO2013003898A1 (en) 2011-07-07 2013-01-10 Clinical Genomics Pty. Ltd Method for detection of cancer in a patient
US9439973B2 (en) * 2012-04-25 2016-09-13 Albert Einstein College Of Medicine, Inc. Transferrin receptor aptamers and aptamer-targeted delivery
HK1213941A1 (zh) * 2012-08-02 2016-07-15 迪肯大学 用於检测癌症干细胞的epcam适配子
SG11201500666TA (en) 2012-08-02 2015-02-27 Univ Deakin Cd133 aptamers for detection of cancer stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103387988A (zh) * 2012-09-24 2013-11-13 厦门大学 上皮细胞粘附分子的核酸适体EpCAM Ccut及其制备方法
WO2014068408A2 (en) * 2012-10-23 2014-05-08 Caris Life Sciences Switzerland Holdings, S.A.R.L. Aptamers and uses thereof
WO2014197455A1 (en) * 2013-06-03 2014-12-11 University Of Florida Research Foundation, Incorporated Devices and methods for isolating cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MCCONNELL, E. et al., "Aptamers as promising molecular recognition elements for diagnostics and therapeutics in the central nervous system", Nucleic Acid Therapeutics, (2014), vol. 24, no. 6, pages 388 - 404 *
SONG, Y. et al., "Selection of DNA aptamers against epithelial cell adhesion molecule for cancer cell imaging and circulating tumor cell capture", Analytical Chemistry, (2013), vol. 85, no. 8, pages 4141 - 4149 *

Also Published As

Publication number Publication date
WO2016127216A1 (en) 2016-08-18
AU2016218945A1 (en) 2017-08-03
US20180037892A1 (en) 2018-02-08
CA2975190A1 (en) 2016-08-18
EP3256593B1 (en) 2020-03-25
US10577610B2 (en) 2020-03-03
JP2018506284A (ja) 2018-03-08
EP3256593A4 (en) 2018-12-05
JP6978317B2 (ja) 2021-12-08
ES2796504T3 (es) 2020-11-27
EP3256593A1 (en) 2017-12-20
CA2975190C (en) 2023-06-06

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