AU2015218576B2 - Methods and compositions for nuclease-mediated targeted integration - Google Patents

Methods and compositions for nuclease-mediated targeted integration Download PDF

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AU2015218576B2
AU2015218576B2 AU2015218576A AU2015218576A AU2015218576B2 AU 2015218576 B2 AU2015218576 B2 AU 2015218576B2 AU 2015218576 A AU2015218576 A AU 2015218576A AU 2015218576 A AU2015218576 A AU 2015218576A AU 2015218576 B2 AU2015218576 B2 AU 2015218576B2
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cell
nuclease
nucleases
aav
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AU2015218576A1 (en
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Michael C. Holmes
Thomas Wechsler
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Sangamo Therapeutics Inc
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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AU2015218576A 2014-02-24 2015-02-24 Methods and compositions for nuclease-mediated targeted integration Ceased AU2015218576B2 (en)

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US201461943865P 2014-02-24 2014-02-24
US61/943,865 2014-02-24
PCT/US2015/017302 WO2015127439A1 (en) 2014-02-24 2015-02-24 Methods and compositions for nuclease-mediated targeted integration

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AU2015218576A1 AU2015218576A1 (en) 2016-09-01
AU2015218576B2 true AU2015218576B2 (en) 2020-02-27

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EP (1) EP3110454B1 (OSRAM)
JP (2) JP6606088B2 (OSRAM)
AU (1) AU2015218576B2 (OSRAM)
CA (1) CA2939942A1 (OSRAM)
WO (1) WO2015127439A1 (OSRAM)

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