AU2014305873B2 - LincRNA-deficient non-human animals - Google Patents

LincRNA-deficient non-human animals Download PDF

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Publication number
AU2014305873B2
AU2014305873B2 AU2014305873A AU2014305873A AU2014305873B2 AU 2014305873 B2 AU2014305873 B2 AU 2014305873B2 AU 2014305873 A AU2014305873 A AU 2014305873A AU 2014305873 A AU2014305873 A AU 2014305873A AU 2014305873 B2 AU2014305873 B2 AU 2014305873B2
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Australia
Prior art keywords
mouse
incrna
pint
nucleic acid
cell
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AU2014305873A
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English (en)
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AU2014305873A1 (en
Inventor
David Frendewey
Guochun Gong
Ka-Man Venus Lai
John Rinn
David M. Valenzuela
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
Harvard University
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Regeneron Pharmaceuticals Inc
Harvard University
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Publication of AU2014305873A1 publication Critical patent/AU2014305873A1/en
Priority to AU2017200183A priority Critical patent/AU2017200183C1/en
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Publication of AU2014305873B2 publication Critical patent/AU2014305873B2/en
Priority to AU2018200281A priority patent/AU2018200281B2/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A01K2217/077—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out heterozygous knock out animals displaying phenotype
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Analytical Chemistry (AREA)
  • Transplantation (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AU2014305873A 2013-08-07 2014-08-07 LincRNA-deficient non-human animals Active AU2014305873B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2017200183A AU2017200183C1 (en) 2013-08-07 2017-01-11 Lincrna-deficient non-human animals
AU2018200281A AU2018200281B2 (en) 2013-08-07 2018-01-12 Lincrna-deficient non-human animals

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361863147P 2013-08-07 2013-08-07
US61/863,147 2013-08-07
PCT/US2014/050178 WO2015021298A2 (en) 2013-08-07 2014-08-07 Lincrna-deficient non-human animals

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2017200183A Division AU2017200183C1 (en) 2013-08-07 2017-01-11 Lincrna-deficient non-human animals

Publications (2)

Publication Number Publication Date
AU2014305873A1 AU2014305873A1 (en) 2016-02-04
AU2014305873B2 true AU2014305873B2 (en) 2017-08-31

Family

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Application Number Title Priority Date Filing Date
AU2014305873A Active AU2014305873B2 (en) 2013-08-07 2014-08-07 LincRNA-deficient non-human animals
AU2017200183A Active AU2017200183C1 (en) 2013-08-07 2017-01-11 Lincrna-deficient non-human animals
AU2018200281A Active AU2018200281B2 (en) 2013-08-07 2018-01-12 Lincrna-deficient non-human animals

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Application Number Title Priority Date Filing Date
AU2017200183A Active AU2017200183C1 (en) 2013-08-07 2017-01-11 Lincrna-deficient non-human animals
AU2018200281A Active AU2018200281B2 (en) 2013-08-07 2018-01-12 Lincrna-deficient non-human animals

Country Status (19)

Country Link
US (3) US9554564B2 (enExample)
EP (2) EP3030653A4 (enExample)
JP (6) JP6998656B2 (enExample)
KR (3) KR102138723B1 (enExample)
CN (2) CN105518132B (enExample)
AU (3) AU2014305873B2 (enExample)
BR (1) BR112016002385A2 (enExample)
CA (1) CA2917714A1 (enExample)
DK (1) DK3178928T3 (enExample)
ES (1) ES2812242T3 (enExample)
HK (1) HK1220990A1 (enExample)
IL (2) IL294443A (enExample)
MX (3) MX369452B (enExample)
MY (2) MY182065A (enExample)
NZ (1) NZ716168A (enExample)
RU (1) RU2721855C2 (enExample)
SG (2) SG11201600036QA (enExample)
WO (1) WO2015021298A2 (enExample)
ZA (1) ZA201600170B (enExample)

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US11064685B2 (en) * 2017-02-27 2021-07-20 Regeneron Pharmaceuticals, Inc. Non-human animal models of retinoschisis
US20200246488A1 (en) * 2017-07-17 2020-08-06 The Broad Institute, Inc. Compositions and methods for treating inflammatory bowel diseases
IL313387A (en) 2017-09-19 2024-08-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
US11932858B2 (en) 2017-12-14 2024-03-19 Donald Danforth Plant Science Center Homologous recombination via transcriptional activation
CN108728545A (zh) * 2018-06-25 2018-11-02 汕头大学医学院附属肿瘤医院 结直肠癌长链非编码rna-hotair分子标志物及其应用
CN108950001B (zh) * 2018-08-31 2020-08-11 青岛泱深生物医药有限公司 一种与直肠腺癌发生发展相关的分子标志物
CN108866198B (zh) * 2018-08-31 2020-08-11 青岛泱深生物医药有限公司 标志物loc105376380在直肠腺癌诊断和治疗中的应用
CN108950000B (zh) * 2018-08-31 2020-08-04 青岛泱深生物医药有限公司 lncRNA在直肠腺癌诊疗中的应用
CN111218451B (zh) * 2020-02-05 2021-08-10 华中农业大学 一种提高猪肌肉量的方法
RU2752663C1 (ru) * 2020-05-18 2021-07-29 ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "СберМедИИ" Способ квантификации статистического анализа альтернативного сплайсинга в данных рнк-сек
WO2022138421A1 (ja) * 2020-12-22 2022-06-30 公立大学法人大阪 Ｘ染色体ノックインモデル動物
USD1010297S1 (en) * 2021-06-30 2024-01-09 Puma SE Shoe
CN113999873B (zh) * 2021-12-31 2022-05-20 北京市疾病预防控制中心 一种基因修饰的非人动物的构建方法及其应用

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Also Published As

Publication number Publication date
EP3030653A2 (en) 2016-06-15
WO2015021298A2 (en) 2015-02-12
ES2812242T3 (es) 2021-03-16
RU2721855C2 (ru) 2020-05-25
DK3178928T3 (da) 2020-08-24
AU2018200281B2 (en) 2020-10-08
CN112715485A (zh) 2021-04-30
EP3178928A1 (en) 2017-06-14
JP2022125255A (ja) 2022-08-26
EP3030653A4 (en) 2017-07-26
US20230189771A1 (en) 2023-06-22
CN105518132B (zh) 2021-02-05
RU2016107434A (ru) 2017-09-15
WO2015021298A3 (en) 2015-04-02
MY198884A (en) 2023-10-02
MX2016001682A (es) 2017-04-13
US11547100B2 (en) 2023-01-10
JP2020108409A (ja) 2020-07-16
MX369452B (es) 2019-11-08
IL243581B (en) 2022-08-01
CN105518132A (zh) 2016-04-20
AU2017200183C1 (en) 2023-04-27
CA2917714A1 (en) 2015-02-12
AU2014305873A1 (en) 2016-02-04
MY182065A (en) 2021-01-18
MX2019013309A (es) 2020-07-28
JP6192865B2 (ja) 2017-09-06
NZ716168A (en) 2022-05-27
US20170071173A1 (en) 2017-03-16
SG11201600036QA (en) 2016-02-26
IL243581A0 (en) 2016-03-31
ZA201600170B (en) 2022-03-30
JP2024164230A (ja) 2024-11-26
EP3178928B1 (en) 2020-06-10
BR112016002385A2 (pt) 2017-09-12
SG10201803766UA (en) 2018-06-28
AU2018200281A1 (en) 2018-02-01
MX2022013409A (es) 2022-11-14
US9554564B2 (en) 2017-01-31
KR102138723B1 (ko) 2020-07-29
US20150047062A1 (en) 2015-02-12
JP2016526924A (ja) 2016-09-08
HK1220990A1 (zh) 2017-05-19
JP2017099407A (ja) 2017-06-08
AU2017200183A1 (en) 2017-02-02
AU2017200183B2 (en) 2018-02-01
KR20160035585A (ko) 2016-03-31
JP6998656B2 (ja) 2022-02-04
KR20170012567A (ko) 2017-02-02
KR101853466B1 (ko) 2018-04-30
IL294443A (en) 2022-09-01
JP2019037253A (ja) 2019-03-14
KR102350200B1 (ko) 2022-01-14
KR20200090977A (ko) 2020-07-29

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