AU2013332956A1

AU2013332956A1 - Inspection chip

Info

Publication number: AU2013332956A1
Application number: AU2013332956A
Authority: AU
Inventors: Yumiko Oshika; Chisato Yoshimura
Original assignee: Brother Industries Ltd
Current assignee: Brother Industries Ltd
Priority date: 2012-10-15
Filing date: 2013-10-15
Publication date: 2015-06-04
Also published as: JP2014081247A; WO2014061635A1

Abstract

Provided is an inspection chip that improves quantification precision. In the inspection chip (2), a virtual plane (H1) that is parallel to a quantitative surface (T) forms an angle (A) with a sample supply unit wall surface (119). A virtual plane (H2) that is parallel to the quantitative surface (T) forms an angle (B) with a first passage wall surface (120). A virtual plane (H3) that is parallel to the quantitative surface (T) forms an angle (C) with a second passage wall surface (122). A virtual plane (H4) that is parallel to the quantitative surface (T) forms an angle (D) with a holding section wall surface (118). The inspection chip (2) is configured so as to satisfy the following relationships: Angle (A) < Angle (B) < Angle (C); Angle (A) < Angle (D); Angle (D) < Angle (B). By satisfying these relationships, it is possible to accurately perform quantification without causing a shortage of a sample when quantifying said sample using a sample quantification unit (114).

Description

Description Title of Invention 5 INSPECTION CHIP TECHNICAL FIELD [0001] The present invention relates to an inspection chip for performing a 0 chemical, medical, or biological inspection of an inspection object. BACKGROUND ART [0002] Conventionally, there have been known inspection chips for inspecting 5 specimens such as biological materials or chemical materials. For example, an inspection chip disclosed in Patent Document I has a quantifying portion which has a V shape as seen from the front side. In this inspection chip, a centrifugal force is applied with respect to the inlet direction of the V-shaped quantifying portion, whereby a specimen or a reagent is injected into the quantifying portion. Next, :0 without changing the direction of the centrifugal force, in the quantifying portion, the specimen or the reagent is quantified. Thereafter, the direction of the centrifugal force is changed, and the quantified specimen or the reagent is used in an inspection. Citation List 5 Patent Literature [0003] Patent Document 1: Japanese Patent Application Laid-Open No. 2009-121912 0 SUM MARY OF T HE INVENTION Problems that the Invention is to Solve [0004] 5 At the quantifying portion of the inspection chip disclosed in Patent Document 1, there is a quantification plane which is a virtual plane where the specimen or the reagent overflows from the quantifying portion. In the quantifying portion, the specimen or the reagent of a volume to the quantification plane is quantified. If a centrifugal force is applied vertically to the quantification plane 0 during injection of the specimen or the reagent into the quantifying portion, due to impact during the injection, the specimen or the reagent scatters to the outside of the quantifying portion. As a result, there is a possibility that, when the injection finishes, the specimen or the reagent injected into the quantifying portion is less than a predetermined quantity. Therefore, there is a problem in which the quantification 5 accuracy decreases. [0005] An object of the present invention is to provide an inspection chip improving the quantification accuracy. :0 Means for Solving the Problems [0006] According to an aspect of the this disclosure, an inspection chip, into which a specimen and a reagent that are liquids are injected and which is rotated around a predetermined first axis such that a centrifugal force is applied, and which is 5 rotated around a second axis different from the first axis such that the direction of the centrifugal force is changed, comprising: a quantifying portion that quantifies the specimen or the reagent; a supplying portion that supplies the specimen or the reagent to the quantifying portion; a first guide portion that guides the specimen or the reagent toward a mixing portion in which the specimen and the reagent are mixed; a 0 second guide portion that guides the specimen or the reagent toward a surplus portion 2 which stores the specimen or the reagent as surplus in the quantifying portion; a first connection portion that is a connection portion of the quantifying portion with the first guide portion connected to the mixing portion; and a second connection portion that is a connection portion of the quantifying portion with the second guide portion 5 connected to the surplus portion, wherein a first angle, which is defined by a direction parallel to a quantification plane connecting the first connection portion and the second connection portion and the extension direction of the wall surface of the supplying portion on the first guide portion side, a second angle, which is defined by a direction parallel to the quantification plane and the extension direction of the wall 0 surface of the first guide portion connected to the first connection portion, and a third angle, which is defined by a direction parallel to the quantification plane and the extension direction of the wall surface of the second guide portion connected to the second connection portion, have the following relation: the first angle < the second angle < the third angle. 5 0007] According to the inspection chip of the above described aspect, during injection of the specimen or the reagent into the quantifying portion, if the centrifugal force is applied to the quantification plane in a direction in which, of angles defined by the quantification plane and the centrifugal force directed to the quantification :0 plane, the second connection portion side becomes an acute angle, it is possible to inject the specimen or the reagent much than the capacity of the quantifying portion. Here, since there is the relation of the first angle < the second angle < the third angle, during movement of the specimen or the reagent in the inspection chip, the specimen or the reagent overflowing out of the quantifying portion does not flow from the first 5 guide portion into the mixing portion and flows from the second guide portion into the surplus portion. Also, during quantification, even if the liquid level is temporally depressed, since the specimen or the reagent much than the capacity of the quantifing portion has been injected, it is possible to improve the quantification accuracy. 0 [0008] 3 The inspection chip may includes a third guide portion that connects the supplying portion and the quantifying portion, and the leading end of the wall surface of the third guide portion on the first guide portion side may be provided on the first guide portion side beyond a position facing the second connection portion. In this 5 case, during injection of the specimen or the reagent into the quantifying portion, since the leading end of the wall surface of the third guide portion on the first guide portion side is provided on the first guide portion side beyond a position facing the second connection portion, it is possible to prevent the specimen or the reagent from flowing from the third guide portion toward the second guide portion, and it is 0 possible to surely inject the specimen or the reagent into the quantifying portion. Therefore, it is possible to prevent the specimen or the reagent injected into the quantifying portion from becoming less than a predetermined quantity and to improve the quantification accuracy. [0009] 5 The inspection chip may further a holding portion that is connected to the supplying portion, and holds the specimen or the reagent supplied to the inspection chip, and the leading end of a holding-portion wall surface which separates the supplying portion and the holding portion may be positioned on the first guide portion side beyond the third guide portion in a direction parallel to the quantification plane. :0 In this case, since the leading end of the holding-portion wall surface is positioned on the first guide portion side beyond the third guide portion in a direction parallel to the quantification plane, when moving the specimen or the reagent from the supplying portion to the holding portion, it is possible to prevent the specimen or the reagent from flowing into the third guide portion and flowing into the quantifying portion. 5 Therefore, during movement of the specimen or the reagent from the supplying portion to the holding portion, it is possible to prevent a decrease in the specimen or the reagent. Therefore, it is possible to prevent the specimen or the reagent injected into the quantifying portion from becoming less than the predetermined quantity and to improve the quantification accuracy. 0 [0010] 4 A fourth angle defined by a direction parallel to the quantification plane and the extension direction of the holding-portion wall surface may be larger than the first angle. In this case, when moving the specimen or the reagent from the holding portion to the supplying portion, it is possible to prevent the specimen or the reagent 5 from flowing into the third guide portion. Therefore, it is possible to prevent the specimen or the reagent from flowing from the third guide portion into the quantifying portion. Therefore, during movement of the specimen or the reagent from the holding portion to the supplying portion, it is possible to prevent a decrease in the specimen or the reagent. Therefore, it is possible to prevent the specimen or 0 the reagent injected into the quantifying portion from becoming less than the predetermined quantity and to improve the quantification accuracy. [0011] The second angle may be larger than the fourth angle. In this case, when moving the specimen or the reagent from the holding portion to the supplying portion, 5 even if the specimen or the reagent flows into the third guide portion, it is possible to prevent the specimen or the reagent from flowing from the first guide portion toward the mixing portion. Therefore, it is possible to prevent the specimen or the reagent injected into the quantifying portion from becoming less than the predetermined quantity and to improve the quantification accuracy. :0 [0012] A step may be formed at the bottom of a passage between the third guide portion and the quantifying portion. In this case, during injection of the specimen or the reagent from the third guide portion into the quantifying portion, the liquid is smoothly guided into the quantifying portion by the step. Therefore, it is possible to 5 prevent the injected specimen or reagent from spreading and obstructing the entrance of the quantifying portion to cause mixing of bubbles in the quantifying portion. Therefore, it is possible to prevent a shortage of the quantified specimen or reagent and to improve the quantification accuracy. [0013] 0 The depth of the third guide portion may be shallower than the depth of the 5 quantifying portion and the depth of the passage between the third guide portion and the quantifying portion. During injection of the specimen or the reagent from the third guide portion into the quantifying portion, the specimen or the reagent flows from the third guide portion whose passage depth is shallow to the deep passage 5 between the third guide portion and the quantifying portion and the deep quantifying portion. Therefore, it is possible to prevent the injected specimen or reagent from spreading and obstructing the entrance of the quantifying portion to cause mixing of bubbles in the quantifying portion. Therefore, it is possible to prevent a shortage of the quantified specimen or reagent and to improve the quantification accuracy. 0 [0014] A neck portion whose depth varies may be formed between the quantifying portion and the passage between the third guide portion and the quantifying portion. In this case, the specimen or the reagent can flow toward the quantifying portion while smoothly changing the flow direction at the neck portion. Therefore, it is 5 possible to prevent a shortage of the quantified specimen or reagent and to improve the quantification accuracy. BRIEF DESCRIPTION OF THE DRAWINGS :0 [0015] FIG. I is a rear view of an inspection device I in which inspection chips 2 are in a normal state. FIG. 2 is a rear view of the inspection device I in which the inspection 5 chips 2 is a rotated state from the normal state. FIG. 3 is a plan view of the inspection device 1 shown in FIG. 1. FIG. 4 is a perspective view of an inspection chip 2. FIG. 5 is a front view of the inspection chip 2 before centrifugal processing. 0 FIG. 6 is an enlarged front view of a specimen quantifying portion 114 and 6 the vicinity thereof. FIG. 7 is a cross-sectional view along a line Y-Y of FIG. 6 as seen from an arrow direction. FIG. 8 is a front view of the inspection chip 2 which is revolved with a 5 rotation angle of 0-degree. FIG. 9 is a front view of the inspection chip 2 which is revolved with a rotation angle of 85-degree. FIG. 10 is an enlarged front view of the specimen quantifying portion 114 of the inspection chip 2 of FIG. 9 which is revolved with the rotation angle of 0 85-degree and the vicinity thereof. FIG. 11 is a front view of the inspection chip 2 which is revolved with the rotation angle of 85-degree. FIG. 12 is a front view of the inspection chip 2 which is revolved with a rotation angle of 90-degree. 5 FIG. 13 is an enlarged front view of the specimen quantifying portion 114 of the inspection chip 2 which is revolved with the rotation angle of 90-degree and the vicinity thereof. FIG. 14 is a front view of the inspection chip 2 which is revolved with the rotation angle of 0-degree. :0 FIG. 15 is an enlarged front view of the specimen quantifying portion 114 of the inspection chip 2 which is revolved with the rotation angle of 90-degree and the vicinity thereof. FIG. 16 is a front view of an inspection chip 102 of a second embodiment. FIG. 17 is a cross-sectional view along a line Z-Z of FIG. 16 as seen from 5 an arrow direction. FIG. 18 is a timing chart illustrating the revolution speed, rotation angle, and specimen movement of the inspection chip 2. 0 EMBODIMENT FOR CARRYING OUT THE INVENTION 7 [0016] Embodiments of the present invention will be described with reference to drawings. The drawings to refer to are used to describe the technical features which 5 the present invention can adopt and are merely illustrative examples. [0017] <1. OUTLINE STRUCTURE OF INSPECTION SYSTEM 3> A first embodiment of this disclosure will be described. The outline structure of an inspection system 3 will be described with reference to FIGS. I and 2. 0 The inspection system 3 of the first embodiment includes inspection chips 2 which can store a specimen and reagents which are liquids, and an inspection device I which performs an inspection using the inspection chips 2. The inspection device 1 can apply a centrifugal force to the inspection chips 2 by rotation around a vertical axis separated from the inspection chip 2. The inspection device I can rotate the 5 inspection chips 2 around a horizontal axis, thereby switching a centrifugal direction which is the direction of a centrifugal force to be applied to the inspection chips 2. [0018] <2. DETAILED STRUCTURE OF INSPECTION DEVICE 1> The detailed structure of the inspection device I will be described with :0 reference to FIGS. I to 3. in the following description, upper side, lower side, right side, left side, near side on a paper and far side on a paper of FIGS. I and 2 are referred to as upper side, lower side, right side, left side, rear side and front side of the inspection device 1, respectively. The upper side, lower side, right side and left side, near side on a paper and far side on a paper of FIG. 3 are referred to as the front 5 side, lower side, right side, left side, upper side and lower side of the inspection device 1, respectively. In the present embodiment, a direction of the vertical axis is a vertical direction, and a direction of the horizontal axis is a speed direction when the inspection chips 2 rotate around the vertical axis. Also, in order to facilitate understanding, in FIGS. I and 2, an upper casing 30 is shown by a virtual line, and in 0 FIG. 3, a state where the top plate of the upper casing 30 has been removed is shown. 8 [0019] As shown in FIGS. I to 3, the inspection device 1 includes the upper casing 30, a lower casing 31, a turntable 33, an angle changing mechanism 34, and a control device 90. The turntable 33 is a disc-like rotator which is provided on the 5 upper surface side of the lower casing 31. The inspection chips 2 are held over the turntable 33. The angle changing mechanism 34 is a driving mechanism provided on the turntable 33. This driving mechanism rotates the inspection chips 2 around the horizontal axes. The upper casing 30 is fixed to the upper side of the lower casing 31 and contains a measuring unit 7 which performs optical measurement on the 0 inspection chips 2. The control device 90 is a controller which controls various processes of the inspection device 1. [0020] The detailed structure of the lower casing 31 will be described. As shown in FIGS. I and 2, the lower casing 31 has a box-shaped frame structure which is a 5 combination of frame materials. At the top of the lower casing 31, a top plate 32 which is a rectangular plate material is provided. On the top plate 32, the turntable 33 is provided so as to be rotatable. Inside the lower casing 31, the driving mechanism for rotating the turntable 33 around the vertical axis is provided as follows. :0 [0021] On the left side of the inside of the lower casing 31, a main spindle motor 35 which supplies a driving force for rotating the turntable 33 is installed. A spindle 36 of the main spindle motor 35 protrudes upward, and a pulley 37 is fixed thereto. At the central portion of the lower casing 31, a vertical main spindle 57 is provided so 5 as to extend upward from the inside of the lower casing 31. The main spindle 57 passes through the top plate 32 and protrudes upward from the lower casing 31. The upper end portion of the main spindle 57 is connected to the central portion of the turntable 33. [0022] 0 The main spindle 57 is rotatably held by a support member 53 provided 9 immediately below the top plate 32. Below the support member 53, a pulley 38 is fixed to the main spindle 57. Over the pulleys 37 and 38, a belt 39 is stretched. If the main spindle motor 35 rotates the spindle 36, a driving force is transmitted to the main spindle 57 through the pulley 37, the belt 39, and the pulley 38. In this case, in 5 consistent with the rotation of the main spindle 57, the turntable 33 rotates around the main spindle 57. [0023] At the right side of the inside of the lower casing 31, a guide rail 56 is installed so as to extend in the vertical axis inside the lower casing 31. A T-shaped 0 plate 48 can move in the vertical direction along the guide rail 56 inside the lower casing 3 1. In a surface of the T-shaped plate 48 on the front side, that is, on the far side on the paper of FIGS. 1 and 2, a groove 80 being long in a left-right direction is formed. [0024] 5 The above described main spindle 57 is a hollow cylinder. An inner shaft 40 is a shaft which is movable in the vertical direction inside the main spindle 57. The upper end portion of the inner shaft 40 is connected to a rack gear 43 (to be described below) through the main spindle 57. At the left end portion of the T-shaped plate 48, a bearing 41 is provided. Inside the bearing 41, the lower portion :0 of the inner shaft 40 is rotatably held. [0025] On the front side of the T-shaped plate 48, a stepping motor 51 for moving the T-shaped plate 48 up and down is fixed. A spindle 58 of the stepping motor 51 protrudes toward the rear side, that is, the side toward the viewer of FIGS. I and 2. 5 At the leading end of the spindle 58, a disc-like cam plate 59 is fixed. On the rear surface of the cam plate 59, a columnar protrusion 70 is formed. The leading end portion of the protrusion 70 is inserted into the above described groove 80. The protrusion 70 can slide in the groove 80. If the stepping motor 51 rotates the spindle 58, the protrusion 70 moves up and down in consistent with rotation of the cam plate 0 59. In this case, in consistent with the protrusion 70 inserted in the groove 80, the 10 T-shaped plate 48 moves up and down along the guide rail 56. [0026] The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 includes a pair of L-shaped plates 60 5 fixed to the top surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed to the vicinity of the center of the turntable 33, and the upper end portion of each L-shaped plate extends outward in the radial direction of the turntable 33. Between the pair of L-shaped plates 60, the rack gear 43 fixed to the inner shaft 40 is provided. The rack gear 43 is a plate-shaped member made of a 0 metal and being long in the vertical direction, and both end surfaces thereof have gear teeth. [0027] On the leading end side of the extension direction of each L-shaped plate 60, a horizontal pivot 46 having a gear 45 is rotatably supported. The pivots 46 are 5 fixed to the inspection chips 2 through mounting holders (not shown). Therefore, in consistent with rotation of the gears 45, the inspection chips 2 also rotate around the pivots 46. Between the gears 45 and the rack gear 43, there are interposed pinion gears 44 supported so as to be rotatable around horizontal axes by the L-shaped plates 60. The pinion gears 44 are engaged with the gears 45 and the rack gear 43. In :0 consistent with vertical movement of the rack gear 43, the pinion gears 44 and the gears 45 are rotated and the inspection chips 2 rotate around the pivots 46. [0028] In the present embodiment, as the main spindle motor 35 rotates the turntable 33, the inspection chips 2 rotate around the main spindle 57 which is a 5 vertical spindle, whereby centrifugal forces are applied to the inspection chips 2. Rotation of the inspection chips 2 around the vertical axis is referred to as revolution. Meanwhile, as the stepping motor 51 moves the inner shaft 40 up and down, the inspection chips 2 rotate around the pivots 46 which are horizontal shafts, whereby the directions of the centrifugal forces to be applied to the inspection chips 2 vary 0 relatively. Rotation of each inspection chip 2 around a horizontal axis is referred to 11 as rotation. [0029] As shown in FIG. 1, in a state where the T-shaped plate 48 has descended to the lowest end of a movable range, the rack gear 43 also descends to the lower end 5 of a movable range. In this case, each inspection chip 2 becomes a normal state in which a rotation angle is 0-degree. As shown in FIG. 2, in the state where the T-shaped plate 48 has ascended to the highest end of the movable range, the rack gear 43 also ascends to the highest end of the movable range. In this case, each inspection chip 2 becomes a state where it has rotated 180-degree around a horizontal 0 axis from the normal state. That is, in the present embodiment, an angle range in which each inspection chip 2 can rotate is from the rotation angle of 0-degree to the rotation angle of 180-degree. [0030] The detailed structure of a upper casing 30 will be described. As shown 5 in FIG. 3, the upper casing 30 has a box-shaped frame structure which is a combination of frame materials and is provided on the left portion of the top plate 32. More specifically, the upper casing 30 is provided outside a range in which the inspection chips 2 are rotated, as seen from the main spindle 57 which is the rotation center of the turntable 33. :0 [0031] A measuring unit 7 provided inside the upper casing 30 includes a light source 71 which emits a measuring right, and an optical sensor 72 which detects the measuring right emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front side and rear side of the turntable 33, on the 5 outside of the rotation range of the inspection chips 2. In the present embodiment, in the revolution range of the inspection chips 2, the left position of the main spindle 57 is a measurement position where each inspection chip 2 is irradiated with the measuring right. In a case where an inspection chip 2 is at the measurement position, the measuring right connecting the light source 71 and the optical sensor 72 intersects 0 with the front and rear surfaces of the inspection chip 2 substantially at a right angle. 12 [0032] <2 STRUCTURE OF INSPECTION CHIP 2> The detailed structure of an inspection chip 2 of the first embodiment will be described with reference to FIGS. 4 to 7. In the following description, the upper 5 side, lower side, left lower side, right upper side, right lower side and left upper side of FIG. 4 are referred to as the upper side, lower side, front side, rear side, right side and left side of the inspection chip 2, respectively. FIGS. 5 to 7 show front views of the inspection chip 2 from which a sheet 29 has been removed. FIGS. 8 to 16 (to be described below) are the same. 0 [0033] As shown in FIG. 4, the inspection chip 2 has, for example, a square shape as seen from the front side and has a transparent synthetic resin plate material 20 having a predetermined thickness, as a main body. The front surface of the plate material 20 is sealed by the sheet 29 made of a thin plate made from a transparent 5 synthetic resin. Between the plate material 20 and the sheet 29, a liquid passage 25 in which liquid sealed in the inspection chip 2 can flow is formed. The liquid passage 25 is a recess formed with a predetermined depth on the front surface side of the plate material 20 and extends in a direction perpendicular to a front-rear direction which is the thickness direction of the plate material 20. That is, the sheet 29 seals :0 the passage formation surface of the plate material 20. [0034] The liquid passage 25 includes a specimen holding portion 111, a specimen quantifying portion 114, a specimen surplus portion 116, a reagent holding portion 131, a reagent quantifying portion 134, a reagent surplus portion 136, a reagent 5 holding portion 151, a reagent quantifying portion 154, a reagent surplus portion 156, and a mixing portion 170. As shown in FIG. 5, the specimen holding portion 111 is a portion, where a specimen 10 is injected and stored. The specimen holding portion 111 is a recess, which is formed between a left side portion 23 and a holding-portion wall surface 118 which is extended toward right oblique upside from the left side 0 portion 23 and which is opened upwardly. The specimen 10 of the present 13 embodiment is, for example, blood. The reagent holding portion 131 is a portion, where a first reagent II is injected and stored. The reagent holding portion 131 is a recess, which is formed between a partition 125 (to be described below) and a holding-portion wall surface 138 which is extended obliquely upward from the 5 partition 125 and which is opened upwardly. The reagent holding portion 151 is a portion, where a second reagent 12 is injected and stored. The reagent holding portion 151 is a recess, which is formed between a partition 145 (to be described below) and a holding-portion wall surface 158 which is extended obliquely upward from the partition 145 and which is opened upwardly. 0 [0035] As shown in FIG. 5, the specimen holding portion 111, the reagent holding portion 131, and the reagent holding portion 151 are formed side by side in the left-right direction on the front surface of the plate material 20, along an upper side portion 21 which is the upper wall surface of the inspection chip 2. Among the 5 specimen holding portion 111, the reagent holding portion 131, and the reagent holding portion 151, the specimen holding portion 111 is the closest to the left side portion 23 which is the left wall surface of the inspection chip 2. The reagent holding portion 151 is the closest to a right side portion 22 which is the right wall surface of the inspection chip 2. The reagent holding portion 131 is positioned :0 between the specimen holding portion 111 and the reagent holding portion 151 Also, the lower wall surface of the inspection chip 2 is a lower side portion 24. [0036] The sheet 29 has a specimen injection hole (not shown) for injecting the specimen 10 into the specimen holding portion 111. For example, the specimen 10 5 contained in a tool (not shown) may be injected from the specimen injection hole by an operation of a user. That is, with using a known method, the specimen 10 may be injected into the specimen holding portion IlI through a reagent injection hole. Similarly, although not shown, the sheet 29 has a reagent injection hole for injecting the first reagent 11 into the reagent holding portion 131, and a reagent injection hole 0 for injecting the first reagent 12 into the reagent holding portion 151. Also, these 14 injection holes may be, for example, holes formed in the upper side portion 21. [0037] A specimen supplying portion 112 is a supply passage of the specimen 10 extending downward from the right upper portion of the specimen holding portion 111. 5 The lower portion of the specimen supplying portion 112 is connected to a specimen guiding portion 113 formed with a narrow passage. Between the specimen supplying portion 112 and the reagent holding portion 131, the partition 125 is formed so as to extend downward from the upper side portion 21. A specimen-supplying-portion wall surface 119 is formed toward left oblique downside from the partition 125. 0 Below the specimen guiding portion 113, the specimen quantifying portion 114 is provided. The specimen quantifying portion 114 is a portion which quantifies the specimen 10 and is a recess with being opened upwardly. [0038] From the connection portion of the specimen guiding portion 113 and the 5 specimen quantifying portion 114, a first passage 117 extends toward the right side and a second passage 115 extends toward the left side. The second passage 115 extends to a specimen surplus portion 116 provided on the left lower side of the specimen quantifying portion 114. That is, the second passage 115 is curved as seen from the front side such that the passage formation direction changes. Therefore, in :0 the second passage 115, in order for the specimen 10 to move between the specimen quantifying portion 114 and the specimen surplus portion 116, it is necessary to apply external forces having a plurality of different directions to the specimen 10. That is, the specimen 10 stored in the specimen surplus portion 116 is difficult to flow back to the specimen quantifying portion 114 through the second passage 115. 5 [0039] The specimen quantifying portion 114 includes two quantification end portion 121 and quantification end portion 123 by which the liquid level of the specimen 10 is formed in a case where the specimen 10 is quantified. The quantification end portion 121 is the connection portion of the specimen quantifying 0 portion 114 and the first passage 117. The quantification end portion 123 is the 15 connection portion of the specimen quantifying portion 114 and the second passage 115. A plane which connects the quantification end portion 121 and the quantification end portion 123 is a quantification plane T shown in FIG. 6. A centrifugal force is applied from the specimen guiding portion 113 toward the inside 5 of the recess of the specimen quantifying portion 114, whereby the same volume of specimen 10 as the volume of the inside of the recess of the specimen quantifying portion 114 is quantified. The details of the quantifying method will be described below. From the quantification end portion 121, a first passage wall surface 120 which forms the first passage 117 toward right oblique upside is provided. From the 0 quantification end portion 123, a second passage wall surface 122 which forms the second passage 115 toward left oblique downside is provided. [0040] The specimen surplus portion 116 is a portion, where the specimen 10 overflowing out of the specimen quantifying portion 114 is stored and is a recess 5 extending toward the right side from the lower portion of the second passage 115. The first passage 117 extends to the mixing portion 170 (to be described below) provided on the right lower side of the specimen quantifying portion 114. That is, in the first passage 117, the passage formation direction changes for the same reason as that of the second passage 115. :0 [0041] A reagent surplus portion 132 is a supply passage of the first reagent 11 extending downward from the right upper portion of the reagent holding portion 131. The lower end portion of the reagent surplus portion 132 is connected to a reagent guiding portion 133 formed with a narrow passage. Between the reagent surplus 5 portion 132 and the reagent holding portion 151, the partition 145 is formed so as to extend downward from the upper side portion 21. From the partition 145, a reagent-supplying-portion wall surface 139 is formed toward left oblique downside. Below the reagent guiding portion 133, the reagent quantifying portion 134 is provided. The reagent quantifying portion 134 is a portion which quantifies the first 0 reagent 11 and is a recess with being opened upwardly. A centrifugal force is 16 applied from the reagent guiding portion 133 toward the inside of the recess of the reagent quantifying portion 134., whereby the same volume of first reagent II as the volume of the inside of the recess of the reagent quantifying portion 134 is quantified. [0042] 5 From the connection portion of the reagent guiding portion 133 and the reagent quantifying portion 134, a third passage 137 extends toward the right side and a fourth passage 135 extends toward the left side. The third passage 137 is connected to the mixing portion 170 (to be described below) provided on the right lower side of the reagent quantifying portion 134. That is, in the third passage 137, 0 the passage formation direction changes for the same reason as that of the first passage 117. The fourth passage 135 extends to the reagent surplus portion 136 provided on the left lower side of the reagent quantifying portion 134. That is, in the fourth passage 135, the passage formation direction changes for the same reason as that of the second passage 115. The reagent surplus portion 136 is a portion, where 5 the first reagent 11 overflowing out of the reagent quantifying portion 134 is stored and is a recess extending toward the right side from the lower end portion of the fourth passage 135. [0043] The reagent quantifying portion 134 includes two quantification end :0 portion 141 and quantification end portion 143 by which the liquid level of the first reagent II is formed in a case where the liquid is quantified. The quantification end portion 141 is the connection portion of the reagent quantifying portion 134 and the third passage 137. The quantification end portion 143 is the connection portion of the reagent quantifying portion 134 and the fourth passage 135. A plane which 5 connects the quantification end portion 141 and the quantification end portion 143 is a quantification plane. A centrifugal force is applied from the reagent guiding portion 133 toward the inside of the recess of the reagent quantifying portion 134, whereby the same volume of first reagent 11 as the volume of the inside of the recess of the reagent quantifying portion 134 is quantified. From the quantification end portion 0 141, a third passage wall surface 140 which forms the third passage 137 toward right 17 oblique upside is provided. From the quantification end portion 143, a fourth passage wall surface 142 which forms the fourth passage 135 toward left oblique downside is provided. [0044] 5 A reagent supplying portion 152 is a supply passage of the second reagent 12 extending downward from the right upper portion of the reagent holding portion 151. The lower end portion of the reagent surplus portion 152 is connected to a reagent guiding portion 153 formed with a narrow passage. Below the reagent guiding portion 153, the reagent quantifying portion 154 is provided. From the right 0 side portion 22, a reagent-suppl ying-portion wall surface 159 is formed toward left oblique downside. The reagent quantifying portion 154 is a portion, which quantifies the second reagent 12 and is a recess with being opened upwardly. A centrifugal force is applied from the reagent guiding portion 153 toward the inside of the recess of the reagent quantifying portion 154, whereby the same volume of the 5 second reagent 21 as the volume of the inside of the recess of the reagent quantifying portion 154 is quantified. [0045] From the connection portion of the reagent guiding portion 153 and the reagent quantifying portion 154, a fifth passage 157 extends toward the right side and :0 a sixth passage 155 extends toward the left side. The sixth passage 155 extends to the reagent surplus portion 156 provided on the left lower side of the reagent quantifying portion 154. That is, in the sixth passage 155, the passage formation direction changes for the same reason as that of the second passage 115. The reagent surplus portion 156 is a portion, where the second reagent 12 overfl owing out of the 5 reagent quantifying portion 154 is stored and is a recess extending toward the right side from the lower end portion of the sixth passage 155. The fifth passage 157 extends to the mixing portion 170 (to be described below) provided on the right lower side of the reagent quantifying portion 154. That is, in the fifth passage 157, the passage formation direction changes for the same reason as that of the second passage 0 115. 18 [0046] The reagent quantifying portion 154 includes two quantification end portion 161 and quantification end portion 163 by which the liquid level of the second reagent 12 is formed in a case where the liquid is quantified. The quantification end 5 portion 161 is the connection portion of the reagent quantifying portion 154 and the fifth passage 157. The quantification end portion 163 is the connection portion of the reagent quantifying portion 154 and the sixth passage 155. A plane which connects the quantification end portion 161 and the quantification end portion 163 is a quantification plane. A centrifugal force is applied from the reagent guiding portion 0 153 toward the inside of the recess of the reagent quantifying portion 154, whereby the same volume of second reagent 12 as the volume of the inside of the recess of the reagent quantifying portion 154 is quantified. From the quantification end portion 161, a fifth passage wall surface 160 which forms the fifth passage 157 toward right oblique upside is provided. From the quantification end portion 163, a sixth passage 5 wall surface 162 which forms the fifth passage 155 toward left oblique downside is provided. [0047] The mixing portion 170 is a rectangular recess provided at the right lower portion of the inspection chip 2 and having the top opened. In the mixing portion :0 170, the specimen 10 introduced from the first passage 117, the first reagent 11 introduced from the third passage 137, and the second reagent 12 introduced from the fifth passage 157 are mixed, whereby a mixed liquid 13 shown in FIG. 15 is generated. The generated mixed liquid 13 is optically measured by a measuring right passing through the mixing portion 170. 5 [0048] With reference to FIG. 6, with respect to virtual planes HI to H4 parallel to the quantification plane T which connects the quantification end portion 121 and the quantification end portion 123, the inclination angle relation of the holding-portion wall surface 118, the specimen-supplying-portion wall surface 119, the first passage 0 wall surface 120, and the second passage wall surface 122 will be described. An 19 angle defined by the virtual plane H1 parallel to the quantification plane T and the specimen-supplying-portion wall surface 119 is referred to as an angle A. An angle defined by the virtual plane H2 parallel to the quantification plane T and the first passage wall surface 120 is referred to as an angle B. An angle defined by the 5 virtual plane 1H3 parallel to the quantification plane ' and the second passage wall surface 122 is referred to as an angle C. An angle defined by the virtual plane 114 parallel to the quantification plane T and the holding-portion wall surface 118 is referred to as an angle D. The inspection chip 2 is configured so as to satisfy the following relations: 0 Angle A < Angle B < Angle C Angle A < Angle D Angle D < Angle B [0049] The specimen-supplying-portion wall surface 119 and the first passage 5 wall surface 120 are formed such that the angle A becomes smaller than the angle B. Due to this angle relation, the inclination angle of the first passage wall surface 120 relative to the virtual plane 1-12 parallel to the quantification plane T becomes larger than the inclination angle of the specimen-supplying-portion wall surface 119 relative to the virtual plane HI parallel to the quantification plane T. Therefore, even when :0 introducing the specimen 10 from the specimen supplying portion 112 into the specimen guiding portion 113 and injecting the specimen 10 into the specimen quantifying portion 114, in case of performing injection by a centrifugal force, an angle defined by the direction of the centrifugal force and the specimen-supplying-portion wall surface 119 is larger than an angle defined by the 5 direction of the centrifugal force and the first passage wall surface 120. Therefore, it is possible to prevent the specimen 10 from flowing along the first passage wall surface 120 and flowing into the mixing portion 170. Also, in case of performing injection by gravity, an angle defined by the direction of the gravity and the specimen-supplying-portion wall surface 119 is larger than an angle defined by the 0 direction of the gravity and the first passage wall surface 120. Therefore, it is 20 possible to prevent the specimen 10 from flowing along the first passage wall surface 120 and flowing into the mixing portion 170. Also, the inclination angle relation of the reagent-supplying-portion wall surface 139 and the third passage wall surface 140 and the inclination angle relation of the reagent-supplying-portion wall surface 159 5 and the fifth passage wall surface 160 are the same as the above described relation. [0050] Also, the first passage wall surface 120 and the second passage wall surface 122 are formed such that the angle B becomes smaller than the angle C. Due to this angle relation, the inclination angle of the second passage wall surface 122 0 relative to the virtual plane -3 parallel to the quantification plane T becomes larger than the inclination angle of the first passage wall surface 120 relative to the virtual plane H2 parallel to the quantification plane T. Therefore, in case of performing injection by a centrifugal force, an angle defined by the direction of the centrifugal force and the second passage wall surface 122 is larger than an angle defined by the 5 direction of the centrifugal force and the first passage wall surface 120. Therefore, the specimen 10 overflowing out of the specimen quantifying portion 114 flows along the second passage wall surface 122 from the second passage 115 into the specimen surplus portion 116, without flowing along the first passage wall surface 120 and flowing into the mixing portion 170. Also, in case of performing injection by gravity, :0 an angle defined by the direction of the gravity and the second passage wall surface 122 is larger than an angle defined by the direction of the gravity and the first passage wall surface 120. Therefore, the specimen 10 overflowing out of the specimen quantifying portion 114 flows along the second passage wall surface 122 from the second passage 115 into the specimen surplus portion 116, without flowing along the 5 first passage wall surface 120 and flowing into the mixing portion 170. Also, the inclination angle relation of the third passage wall surface 140 and the fourth passage wall surface 142, and the effect thereof are the same as described above. The inclination angle relation of the fifth passage wall surface 160 and the sixth passage wall surface 162, and the effect thereof also are the same as described above. 0 [0051] 21 Also, the holding-portion wall surface 118 and the specimen-supplying-portion wall surface 119 are formed such that the angle A becomes smaller than the angle D. Due to this angle relation, the inclination angle of the holding-portion wall surface 118 relative to the virtual plane H4 parallel to the 5 quantification plane T becomes larger than the inclination angle of the specimen-supplying-portion wall surface 119 relative to the virtual plane HI parallel to the quantification plane T. When moving the specimen from the specimen holding portion 111 to the specimen supplying portion 112 by a centrifugal force, an angle defined by the direction of the centrifugal force and the holding-portion wall surface 0 118 is larger than an angle defined by the direction of the centrifugal force and the specimen-supplying-portion wall surface 119. Therefore, when moving the specimen 10 from the specimen holding portion 111 to the specimen supplying portion 112, it is possible to prevent the specimen 10 from flowing into the specimen guiding portion 113. Also, when moving the specimen from the specimen holding portion 5 111 to the specimen supplying portion 112 by gravity, an angle defined by the direction of the gravity and the holding-portion wall surface 118 is larger than an angle defined by the direction of the gravity and the specimen-supplying-portion wall surface 119. Therefore, when moving the specimen 10 from the specimen holding portion 111 to the specimen supplying portion 112, it is possible to prevent the :0 specimen 10 from flowing into the specimen guiding portion 113. The inclination angle relation of the holding-portion wall surface 138 and the reagent-supplying-portion wall surface 139, and the effect thereof also are the same as described above. The inclination angle relation of the holding-portion wall surface 158 and the reagent-supplying-portion wall surface 159, and the effect thereof also are 5 the same as described above. [0052] Also, the holding-portion wall surface 118 and the first passage wall surface 120 are formed such that the angle D becomes smaller than the angle B. Due to this angle relation, the inclination angle of the first passage wall surface 120 0 relative to the virtual plane H2 parallel to the quantification plane T becomes larger 22 than the inclination angle of the holding-portion wall surface 118 relative to the virtual plane 114 parallel to the quantification plane T. When moving the specimen from the specimen holding portion III to the specimen supplying portion 112 by a centrifugal force, an angle defined by the direction of the centrifugal force and the 5 first passage wall surface 120 is larger than an angle defined by the direction of the centrifugal force and the holding-portion wall surface 118. Therefore, when moving the specimen 10 from the specimen holding portion 111 to the specimen supplying portion 112, even if the specimen 10 flows into the specimen guiding portion 113 and leaks into the first passage, it is possible to prevent the specimen 10 from flowing 0 along the first passage wall surface 120 toward the mixing portion 170. Also, when moving the specimen from the specimen holding portion I1 to the specimen supplying portion 112 by gravity, an angle defined by the direction of the gravity and the first passage wall surface 120 is larger than an angle defined by the direction of the gravity and the holding-portion wall surface 118. Therefore, when moving the 5 specimen 10 from the specimen holding portion 111 to the specimen supplying portion 112, even if the specimen 10 flows into the specimen guiding portion 113 and leaks into the first passage, it is possible to prevent the specimen 10 from flowing along the first passage wall surface 120 toward the mixing portion 170. Also, the inclination angle relation of the holding-portion wall surface 138 and the third passage wall :0 surface 140, and the effect thereof also are the same as described above. The inclination angle relation of the holding-portion wall surface 158 and the fifth passage wall surface 160, and the effect thereof also are the same as described above. [0053] Further, as shown in FIG. 6, the leading end 113A of a wall surface of the 5 specimen guiding portion 113 positioned on the first passage (117) side is formed on the first passage (117) side, from a position facing the quantification end portion 123, in the left-right direction which is the direction of a virtual plane parallel to the quantification plane T. Therefore, it is possible to prevent the specimen 10 which is injected from the specimen guiding portion 113 into the specimen quantifying portion 0 114 from flowing into the second passage 115 without entering the specimen 23 quantifying portion 114. If the specimen 10 is injected toward the second passage 115, bubbles accumulated in the specimen quantifying portion 114 of the second passage (115) side are discharged, and the quantification accuracy decreases. In order to avoid this, the specimen 10 is injected toward the first passage 117. 5 Therefore, it is possible to prevent a shortage of the quantity of the specimen 10 in the specimen quantifying portion 114. Also, the reagent guiding portion 133 and the reagent guiding portion 153 have the same structure as described above, and can achieve the same effects. [0054] 0 Also, as shown in FIG. 6, the leading end 118A of the holding-portion wall surface 118 is positioned on the first passage (117) side beyond the specimen guiding portion 113 in the left-right direction. Therefore, when applying a centrifugal force X in an arrow direction shown in FIG. 8, thereby moving the specimen 10 from the specimen holding portion 111 to the specimen supplying portion 112, since the 5 leading end 118A of the holding-portion wall surface 118 is positioned on the downstream side of the centrifugal force from the specimen guiding portion 113, it is possible to prevent the specimen 10 from flowing into the specimen guiding portion 113 and flowing into the specimen quantifying portion 114. [0055] :0 Next, with reference to the cross-sectional view of FIG. 7, the shapes of the specimen guiding portion 113, and the bottom 124A of a passage 124 between the specimen guiding portion 113 and the specimen quantifying portion 114 will be described. The bottom 124A of the passage 124 between the specimen guiding portion 113 and the specimen quantifying portion 114 is a flat surface having the same 5 depth as the bottom 114A of the specimen quantifying portion 114. Further, the depth LI of the specimen guiding portion 113 is shallower than the depth L2 of the bottom 124A of the passage 124. [0056] <3. THE OTHER STRUCTURE OF INSPECTION CHIP 2> 0 As shown in FIG. 1, the pivots 46 extending from the L-shaped plates 60 24 are connected vertically to the centers of the rear surfaces of the plate materials 20 through the mounting holders (not shown). According to rotation of the pivots 46., the inspection chips 2 rotate on the pivots 46. In a case where an inspection chip 2 is in the normal state shown in FIGS. 4 to 6, the upper side portion 21 and the lower side 5 portion 24 are disposed so as to be perpendicular to the direction of gravity G, and the right side portion 22 and the left side portion 23 are disposed in parallel to the direction of gravity G, and the left side portion 23 is disposed on the main spindle (57) side beyond the right side portion 22. In a case where an inspection chip 2 in the normal state is disposed at the measurement position, the measuring right 0 connecting the light source 71 and the optical sensor 72 vertically passes through the mixing portion 170 as seen from the above. [0057] <4. EXAMPLE OF INSPECTION METHOD> With reference to FIGS. 5 to 15, and 18, an inspection method using the 5 inspection device I and an inspection chip 2 will be described. Also, control on the rotation, revolution, and rotation speed of the inspection chip 2 shown in FIG. 18 is performed by whether each of times TI to T5 has elapsed. Information on the times TI to T5 is stored in advance in a storage device (not shown) of the control device 90. If the user attaches the inspection chip 2 to a pivot 46, and inputs a process start :0 command to the control device 90, the following measuring operation is performed. The input time of the process start command is a time TO of the timing chart shown in FIG. 18. Also, the inspection device I can simultaneously inspect two inspection chips 2. However, hereinafter, for convenience of explanation, a procedure of inspecting one inspection chip 2 will be described. In the following description, the 5 normal state of the inspection chip 2 shown in FIG. 5 means that the rotation angle is 0-degree, and a state where the inspection chip has rotated 85-degree counterclockwise from the normal state is referred to as the rotation angle of 85-degree. A state where the inspection chip has further rotated 5-degree counterclockwise from the rotation angle of 85-degree is referred to as the rotation 0 angle of 90-degree. 25 [0058] First, based on an instruction of the control device 90, from the time TO, the main spindle motor 35 starts driving of the turntable 33. As a result, the inspection chip 2 with the rotation angle of 0-degree revolves. The main spindle 5 motor 35 increases the rotation speed of the turntable 33 based on an instruction of the control device 90. If the rotation speed reaches 3000 rpm, the main spindle motor 35 maintains that rotation speed. Hereinafter, the state where the turntable 33 is rotating at the rotation speed of 3000 rpm will be referred to as high-speed driving. Also, the rotation speed during high-speed driving is not limited to 3000 rpm, and 0 may be any other rotation speed. During a period from the time TO to the time TI, as shown in FIG. 8, from the left side portion 23 toward the right side portion 22, the centrifugal force X acts on the inspection chip 2. Due to the action of the centrifugal force X, the specimen 10 moves from the specimen holding portion 1II to the specimen supplying portion 112. Similarly, the first reagent 11 moves from the 5 reagent holding portion 131 to the reagent surplus portion 132. The second reagent 12 moves from the reagent holding portion 151 to the reagent supplying portion 152. The time TI is set to a time sufficient for moving the specimen 10, the first reagent 11 and the second reagent 12 as described above and is stored in advance in the storage device (not shown) of the control device 90. :0 [0059] At the time TI, according to driving control on the stepping motor 51 based on an instruction of the control device 90, as shown in FIG. 9, the inspection chip 2 revolving according to high-speed driving is rotated 85-degree counterclockwise as seen from the front side. Therefore, the rotation angle of the 5 inspection chip 2 changes into 85-degree. As a result, a centrifugal force X1 acts on the inspection chip 2 such that an angle defined by the upper side portion 21 and the direction of the centrifugal force becomes 85-degree. Here, as shown in FIG. 10, of angles defined by the quantification plane T and the direction of the acting centrifugal force XI, an angle E on the quantification end portion (123) side is an acute angle and 0 an angle F adjacent to the angle E is an obtuse angle. Since the rotation angle of the 26 inspection chip 2 is 85-degree, the angle E becomes 85-degree. As shown in FIG. 9, due to the action of the centrifugal force XI, the specimen 10 flows into the specimen quantifying portion 114 through the specimen guiding portion 113. Similarly, the first reagent 11 flows into the reagent quantifying portion 134 through the reagent 5 guiding portion 133. The second reagent 12 flows into the reagent quantifying portion 154 through the reagent guiding portion 153. [0060] Next, during a period from the time TI to the time T2, based on an 0 instruction of the control device 90, the main spindle motor 35 decreases the rotation speed of the turntable 33. If the rotation speed reaches 1000 rpm, the main spindle motor 35 maintains that rotation speed. Hereinafter, the state where the turntable 33 is rotating at the rotation speed of 1000 rpm will be referred to as low-speed driving Also, the rotation speed during low-speed driving is not limited to 1000 rpm, as long 5 as it is lower than the rotation speed during high-speed driving, and may be any other rotation speed. The centrifugal force X during low-speed driving is weaker than the centrifugal force X during high-speed driving. That is, the centrifugal force X at the time T2 is weaker than the centrifugal force X during start of injection of the individual liquids into the specimen quantifying portion 114, the reagent quantifying :0 portion 134, and the reagent quantifying portion 154. Therefore, during start of injection into the specimen quantifying portion 114, the reagent quantifying portion 134, and the reagent quantifying portion 154, the individual liquids are efficiently injected by the storing centrifugal force, and during finishing of the injection, the centrifugal force weakens, whereby, it is possible to prevent mixing of bubbles and 5 suppress depression of the liquid level of each liquid. The time T2 is set to a time sufficient for moving the specimen 10, the first reagent 11 and the second reagent 12 to the individual quantifying portions and is stored in advance in the storage device (not shown) of the control device 90. [0061] 0 As shown in FIG. 10, the specimen guiding portion 113 guides and 27 supplies the specimen 10 toward the specimen quantifying portion 114. The first passage 117 may be positioned in the direction of the centrifugal force X1 at the downstream-side leading end 113A of the specimen guiding portion 113. In this case, of angles defined by the direction of the centrifugal force X1 and the extension 5 direction J of the specimen guiding portion 113, an angle K on the specimen quantifying portion (114) side is an acute angle. [0062] As shown in FIG. 10, the specimen 10 exceeding a predetermined quantity in the specimen quantifying portion 114 overflows into the second passage 115 and is 0 stored in the specimen surplus portion 116. Here, since the centrifugal force X1 acts such that the angle E on the quantification end portion (123) side becomes an acute angle, the specimen 10 becomes the total amount of the volume of the specimen quantifying portion 114 and a quantity overflowing over the quantification end portion 121 toward the first passage wall surface 120. This is the same even in the 5 reagent quantifying portion 134 and the reagent quantifying portion 154. That is, during the period from the time Ti to the time T2 shown in FIG. 18, the main spindle motor 35 drives the turntable 33 at a low speed, thereby making the centrifugal force weaker than the centrifugal force during high-speed driving. This is because if the centrifugal force is strong, the liquid level is depressed in the direction of the :0 centrifugal force, and thus the quantity of the total amount of the specimen 10 decreases. [0063] Next, at the time T2 shown in FIG. 18, according to driving control on the stepping motor 51 based on an instruction of the control device 90, the inspection chip 5 2 revolving according low-speed driving is rotated 5-degree counterclockwise as seen from the front side. Therefore, as shown in FIG. 12, the rotation angle of the inspection chip 2 changes into 90-degree. As shown in FIG. 13, with respect to an acting centrifugal force X2 relative to the quantification plane T, an angle G on the quantification end portion (123) side becomes 90-degree and an angle H adjacent to 0 the angle G also becomes 90-degree. Therefore, as shown in FIG. 13, the specimen 28 10 exceeding the quantification plane T and overflowing over the quantification end portion 121 toward the first passage wall surface 120 overflows into the second passage 115. The overflowed specimen 10 is stored in the specimen surplus portion 116. As a result, the predetermined amount of specimen 10 is accurately quantified. 5 Fere, the volume surrounded by the quantification plane T and the wall forming the specimen quantifying portion 114 is a desired quantity. The specimen 10 of the volume quantified in the specimen quantifying portion 114 is mixed with the first reagent 11 and the second reagent 12, whereby the mixed liquid is generated. This mixed liquid is optically measured. If the volume quantified in the specimen holding 0 portion 111 is deviated from a desired volume, the inspection accuracy based on optical measurement decreases. Similarly, the first reagent 11 exceeding a predetermined quantity overflows into the fourth passage 135. The overflowing first reagent 11 is stored in the reagent surplus portion 136. As a result, a desired quantity of first reagent 11 is accurately quantified. Also, in the reagent quantifying 5 portion 154, the second reagent 12 exceeding a predetermined quantity overflows into the sixth passage 155. The overflowing second reagent 12 is stored in the reagent surplus portion 156. As a result, a desired quantity of second reagent 12 is accurately quantified. [0064] :0 Next, during a period from the time T2 to the time T3 shown in FIG. 18, based on an instruction of the control device 90, the main spindle motor 35 drives the turntable 33 at a high speed. As a result, the inspection chip 2 with the rotation angle of 90-degree revolves. According to this high-speed driving, a viscous specimen such as blood is more accurately quantified by a centrifugal force stronger 5 than the centrifugal force during low-speed driving. The time T3 is set to a time sufficient for quantifying the specimen 10, the first reagent 11 and the second reagent 12 in the individual quantifying portions and is stored in advance in the storage device (not shown) of the control device 90. [0065] 0 Next, at the time T3 shown in FIG. 18, according to driving control on the 29 stepping motor 51 based on an instruction of the control device 90, as shown in FIG. 14, the inspection chip 2 being revolving is rotated 90-degree clockwise as seen from the front side. As a result, the rotation angle of the inspection chip 2 returns to 0-degree, and the centrifugal force X acts on the inspection chip 2 from the left side 5 portion 23 toward the right side portion 22. Due to the action of the centrifugal force X, the specimen 10 quantified in the specimen quantifying portion 114 moves to the first passage 117. Meanwhile, since the specimen surplus portion 116 is a recess being closed at the right side, the surplus specimen 10 remains in the specimen surplus portion 116. 0 [0066] Similarly, the first reagent 11 quantified in the reagent quantifying portion 134 moves to the fourth passage 135. Meanwhile, since the reagent surplus portion 136 is a recess being closed at the right side, the surplus first reagent 11 remains in the reagent surplus portion 136. The second reagent 12 quantified in the reagent 5 quantifying portion 154 moves to the fifth passage 157. Meanwhile, since the reagent surplus portion 156 is a recess being closed at the right side, the surplus second reagent 12 remains in the reagent surplus portion 156. [0067] Next, at the time T4 shown in FIG. 18, according to driving control on the :0 stepping motor 51 based on an instruction of the control device 90, as shown in FIGT. 15, the inspection chip 2 being revolving is rotated 90-degree counterclockwise as seen from the front side. As a result, the rotation angle of the inspection chip 2 changes into 90-degree, the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the 5 centrifugal force X, the specimen 10 flows from the first passage 117 into the mixing portion 170. The first reagent 11 flows from the third passage 137 into the mixing portion 170. The second reagent 12 flows from the third passage 157 into the mixing portion 170. Meanwhile, the surplus specimen 10, the surplus first reagent 11, and the surplus second reagent 12 remain in the specimen surplus portion 116, the reagent 0 surplus portion 136, and the reagent surplus portion 156 as described above. The 30 specimen 10, the first reagent 11, and the second reagent 12 introduced into the mixing portion 170 is mixed by action of the centrifugal force X, whereby the mixed liquid 13 is generated. [0068] 5 Next, at the time T5 shown in FIG. 18, according to driving control on the stepping motor 51 based on an instruction of the control device 90, the inspection chip 2 being revolving is rotated 90-degree clockwise as seen from the front side. As a result, the rotation angle of the inspection chip 2 changes into 0-degree. Thereafter, according to driving control on the main spindle motor 35, the main spindle motor 35 0 is decelerated, and the main spindle motor 35 stops. Therefore, the revolution of the inspection chip 2 finishes. [0069] After performance of the above described centrifugal processing, according to driving control on the main spindle motor 35, the inspection chip 2 is 5 rotated to the angle of the measurement position. If the light source 71 emits a measuring right, the measuring right passes through the mixed liquid 13 stored in the mixing portion 170. Based on the amount of change of the measuring right received by the optical sensor 72, optical measurement on the mixed liquid 13 is performed, and measurement data is acquired. Next, based on the acquired measurement data, :0 the measurement result of the specimen 10 is calculated. The inspection result of the specimen 10 based on the measurement result is displayed. Thereafter, the main process is finished. [0070] <5. MAIN ACTIONS/EFFEC 1S OF FIRST FMBO(1) DIME NT> 5 As described above, according to an inspection chip 2 and the inspection device I of the first embodiment, the inspection device I can use an inspection chip 2 into which the specimen 10, the first reagent 11, and the second reagent 12 which are liquids have been injected. In the inspection chip 2, the specimen quantifying portion 114 can contain a predetermined quantity of specimen 10 introduced into the 0 inspection chip 2. The specimen 10 exceeding the predetermined quantity overflows 31 from the specimen quantifying portion 114, moves in the second passage 115 and is stored in the specimen surplus portion 116. During injection of the specimen into the specimen quantifying portion 114, the rotation angle of the inspection chip 2 is set to 85-degree, and the centrifugal force X acting on the quantification plane T acts 5 such that the angle E on the quantification end portion (123) side becomes an acute angle of 85-degree. Therefore, since the centrifugal force is not applied in a direction perpendicular to the quantification plane T, it is possible to suppress the liquid level from being depressed during injection of the specimen or a reagent. Also, the specimen 10 becomes the total amount of the volume of the specimen 0 quantifying portion 114 and the amount overflowing over the quantification end portion 121 toward the first passage wall surface 120. Therefore, it is possible to inject the specimen or a reagent much than the capacity of the specimen quantifying portion 114. This is the same even in the reagent quantifying portion 134 and the reagent quantifying portion 154. Thereafter, since the rotation angle of the 5 inspection chip 2 is set to 90-degree, and quantification is performed, during quantification, the centrifugal force is applied from a direction perpendicular to the quantification plane T. However, for example, even if the liquid level is depressed, since the liquid much than the volume of the specimen quantifying portion 114 is injected, it is possible to accurately perform quantification without a shortage of the :0 liquid quantity. [0071] During quantification, the turntable 33 rotates at the rotation speed of 3000 rpm, and the centrifugal force stronger than that during start of injection of the specimen into the specimen quantifying portion 114 is applied to the quantification 5 plane T. Therefore, it is possible to perform quantification in a short time. In this case, during injection of the specimen or a reagent is superfluously injected into a quantifying portion. Therefore, during quantification, quantification is performed by a strong centrifugal force, and even if the liquid level is depressed, the quantification accuracy does not decrease. 0 [0072] 32 According to an angle difference between the direction of the centrifugal force acting on the quantification plane during injection of the specimen into the specimen quantifying portion 114 and the direction of the centrifugal force acting on the quantification plane during quantification, during quantification, a revolution 5 controller (not shown) controls the rotation speed of the main spindle motor 35. Therefore, since the quantity of the specimen or a reagent superfluously injected into the quantifying portion according to the angle difference between the corresponding centrifugal forces varies, a strong centrifugal force is applied according to the quantity superfluously injected, whereby it is possible to perform quantification in a 0 short time. [0073] During injection of the specimen into the specimen quantifying portion 114, the revolution controller (not shown) drives the main spindle motor 35 at a high speed, and then performs low-speed driving at a rotation speed lower than that during 5 high-speed driving. Therefore, it is possible to decrease the injection time, and it is possible to prevent the injected specimen or reagent from excessively flowing into the mixing portion or a surplus portion. [0074] The first passage 117 is positioned on the direction side to which the :0 centrifugal force X1 is directed, at the downstream side leading end 113A of the specimen guiding portion 113 supplying the specimen or a reagent toward the specimen quantifying portion 114, and of angles defined by the direction to which the centrifugal force X1 is directed and the extension direction J of the specimen guiding portion 113, the angle K of the specimen quantifying portion 114 becomes an acute 5 angle. Therefore, if the specimen or a reagent is poured onto the first passage wall surface 120 of the first passage 117 so as to flow into the specimen quantifying portion 114, it is possible to generate convection in the specimen quantifying portion 114. Therefore, the specimen or the reagent injected into the specimen quantifying portion 114 becomes homogeneous. 0 [0075] 33 <6. SECOND EMBODIMENT OF INSPECTION CHIP> Next, with reference to FIGS. 16 and 17, an inspection chip 102 which is a second embodiment of the inspection chip will be described. Here, only differences of the inspection chip 102 of the secondary electron from the inspection chip 2 of the 5 first embodiment will be described, and parts having structures identical to those of the inspection chip 2 are denoted by the same reference symbols, and will not be described. [0076] As shown in FIGS. 16 and 17, in the inspection chip 102, the depth L2 of 0 the passage 124 between the specimen guiding portion 113 and the specimen quantifying portion 114 is deeper than the depth L3 of the specimen quantifying portion 114 and the depth Li of the specimen guiding portion 113. The depth LI of the specimen guiding portion 113 is the deepest. Also, from the bottom 124A of the passage 124, a step 126 which is an inclined surface is formed toward the bottom 5 114A of the specimen quantifying portion 114. Further, between the bottom 124A of the passage 124 and the step 126, a neck portion 127 whose depth varies is formed. Even in a portion between the reagent guiding portion 133 and the reagent quantifying portion 134, a step 146 is formed. Even in a portion between the reagent guiding portion 153 and the reagent quantifying portion 154, a step 166 is formed. :0 [0077] <7. MAIN ACTIONS/EFFECTS OF SECOND EMBODIMENT> As described above, according to the inspection chip 102 of the second embodiment, from the bottom 124A of the passage 124 between the specimen guiding portion 113 and the specimen quantifying portion 114, the step 126 which is an 5 inclined surface is formed toward the bottom 114A of the specimen quantifying portion 114. Therefore, during injection of the specimen from the specimen guiding portion 113 into the specimen quantifying portion 114, it is possible to prevent the liquid from spreading in the left-right direction and obstructing the entrance of the specimen quantifying portion 114 to cause mixing of bubbles in the quantifying 0 portion. Therefore, it is possible to prevent a shortage of the quantified specimen. 34 [0078] Also, in the inspection chip 102, the depth LI of the specimen guiding portion 113 is shallower than the depth L2 of the passage 124 between the specimen guiding portion 113 and the specimen quantifying portion 114 and the depth L3 of the 5 specimen quantifying portion 114. Therefore, since the specimen flows from the specimen guiding portion 113 whose passage depth is shallow into the passage 124 and the specimen quantifying portion 114 whose passage depths are deep, during injection of the specimen from the specimen guiding portion 113 into the specimen quantifying portion 114, it is possible to prevent the liquid from spreading and 0 obstructing the entrance of the specimen quantifying portion 114 to cause mixing of bubbles in the quantifying portion. [0079] Also, in the inspection chip 102, between the bottom 124A of the passage 124 and the step 126, the neck portion 127 whose depth varies is formed. Therefore, 5 the specimen can flow into the specimen quantifying portion 114 while smoothly changing the flow direction. Also, even in the passage between the reagent guiding portion 133 and the reagent quantifying portion 134, and the passage between the reagent guiding portion 153 and the reagent quantifying portion 154, the same actions and effects are achieved. :0 [0080] <8. OTHERS> In the above described embodiments, each of the specimen holding portion 111, the reagent holding portion 131, and the reagent holding portion 151 is an example of a "holding portion" of this disclosure. Each of the holding-portion wall 5 surface 118, the holding-portion wall surface 138, and the holding-portion wall surface 158 is an example of a "holding-portion wall surface" of this disclosure. Each of the specimen supplying portion 112, the reagent surplus portion 132, and the reagent supplying portion 152 is an example of a "supplying portion" of this disclosure. Each of the specimen quantifying portion 114, the reagent quantifying 0 portion 134, and the reagent quantifying portion 154 is an example of a "quantifying 35 portion" of this disclosure. Each of the first passage 117, the third passage 137, and the fifth passage 157 is an example of a "first guide portion" of this disclosure. Each of the second passage 115, the fourth passage 135, and the sixth passage 155 is an example of a "second guide portion" of this disclosure. Each of the specimen 5 guiding portion 113, the reagent guiding portion 133, and the reagent guiding portion 153 is an example of a "third guide portion" of this disclosure. Each of the specimen surplus portion 116, the reagent surplus portion 136, and the reagent surplus portion 156 is an example a "surplus portion" of this disclosure. The mixing portion 170 is an example of a "mixing portion" of this disclosure. Each of the quantification end 0 portion 121, the quantification end portion 141, and the quantification end portion 161 is an example of a "first connection portion" of this disclosure. Each of the quantification end portion 123, the quantification end portion 143, and the quantification end portion 163 is an example of a "second connection portion" of this disclosure. The quantification plane T is an example of a "quantification plane" of 5 this disclosure. Each of the step 126, the step 146, and the step 166 is an example of a "step" of this disclosure. The neck portion 127 is an example of a "neck portion" of this disclosure. [0081] The angle A is an example of a "first angle" of this disclosure. The angle :0 B is an example of a "second angle" of this disclosure. The angle C is an example of a "third angle" of this disclosure. The angle D is an example of a "fourth angle" of this disclosure. The specimen 10 is an example of a "specimen" of this disclosure. Each of the first reagent 11 and the second reagent 12 is an example of a "reagent" of this disclosure. 5 [0082] This disclosure is not limited to the above described embodiments, and various modifications can be made. The inspection device I and the inspection chips 2 of the above described embodiments are merely illustrative, and the structure, shape, processing, and the like of each of them can be modified. For example, the rotation 0 angle of each inspection chip 2 for applying a first centrifugal force to the 36 quantification plane T is not limited to 85-degree, and may be an arbitrary acute angle such as 80-degree, 75-degree, 70-degree, or 65-degree. Also, the rotation angle of each inspection chip 2 for applying a second centrifugal force to the quantification plane T is not limited to 90-degree. That is, the rotation angle of each inspection 5 chip 2 needs only to be set such that a second centrifugal force is applied to the quantification plane in a direction in which the absolute value of the difference between two adjacent angles of angles defined by the quantification plane T and the second centrifugal force directed to the quantification plane T becomes smaller than the absolute value of the difference between two adjacent angles of angles defined by 0 the quantification plane T and the first centrifugal force directed to the quantification plane T. [0083] Also, as shown in FIG. 10, of the angles defined by the direction of the centrifugal force X1 and the extension direction J of the specimen guiding portion 113, 5 the angle K of the specimen quantifying portion 114 may be 0-degree, not an acute angle. Description of Reference Numerals and Symbols I INSPECTION DEVICE 0 2 INSPECTION CHIP 7 MEASURING PORTION 10 SPECIMEN II FIRST REAGENT 12 SECOND REAGENT 513 MIXED LIQUID 114 SPECIMEN QUANTIFYING PORTION 115 SECOND PASSAGE 117 FIRST PASSAGE 116 SPECIMEN SURPLUS PORTION 0 121, 141, 161 QUANTIFICATION END PORTION 37 123, 143, 163 QUANTIFICATION END PORTION 127 NECK PORTION 134 REAGENT QUANTIFYING PORTION 154 REAGENT QUANTIFYING PORTION 5 170 MIXING PORTION 126, 146, 166 STEP 38

Claims

1. An inspection chip, into which a specimen and a reagent that are liquids are injected and which is rotated around a predetermined first axis such that a 5 centrifugal force is applied, and which is rotated around a second axis different from the first axis such that the direction of the centrifugal force is changed, comprising: a quantifying portion that quantifies the specimen or the reagent; a supplying portion that supplies the specimen or the reagent to the quantify ying portion; 0 a first guide portion that guides the specimen or the reagent toward a mixing portion in which the specimen and the reagent are mixed; a second guide portion that guides the specimen or the reagent toward a surplus portion which stores the specimen or the reagent as surplus in the quantifying portion; 5 a first connection portion that is a connection portion of the quantifying portion with the first guide portion connected to the mixing portion; and a second connection portion that is a connection portion of the quantifying portion with the second guide portion connected to the surplus portion, wherein :0 a first angle, which is defined by a direction parallel to a quantification plane connecting the first connection portion and the second connection portion and the extension direction of the wall surface of the supplying portion on the first guide portion side, a second angle, which is defined by a direction parallel to the 5 quantification plane and the extension direction of the wall surface of the first guide portion connected to the first connection portion, and a third angle, which is defined by a direction parallel to the quantification plane and the extension direction of the wall surface of the second guide portion connected to the second connection portion, have the following relation: 0 the first angle < the second angle < the third angle. 39

2. The inspection chip according to claim I, further comprising: a third guide portion that connects the supplying portion and the quantifying portion, 5 wherein the leading end of the wall surface of the third guide portion on the first guide portion side is provided on the first guide portion side beyond a position facing the second connection portion.

3. The inspection chip according to claim 2, further comprising: 0 a holding portion that is connected to the supplying portion and holds the specimen or the reagent supplied to the inspection chip, wherein the leading end of a holding-portion wall surface which separates the supplying portion and the holding portion is positioned on the first guide portion side beyond the third guide portion in a direction parallel to the quantification plane. 5

4. The inspection chip according to claim 3, wherein a fourth angle defined by a direction parallel to the quantification plane and the extension direction of the holding-portion wall surface is larger than the first angle. :0

5. The inspection chip according to claim 4, wherein the second angle is larger than the fourth angle.

6. The inspection chip according to claim 2, 5 wherein a step is formed at the bottom of a passage between the third guide portion and the quantifying portion.

The inspection chip according to claim 2,: wherein the depth of the third guide portion is shallower than the depth of 0 the quantifying portion and the depth of the passage between the third guide portion 40 and the quantifying portion.

8. The inspection chip according to claim 2, wherein a neck portion, whose depth varies, is formed between the 5 quantifying portion and the passage between the third guide portion and the quantifying portion. 41