JP6036666B2 - Inspection chip - Google Patents

Description

  The present invention relates to an inspection chip having flow paths formed on a front surface and a back surface, and provided with a hole portion that connects the front surface flow path and the back surface flow path.

  2. Description of the Related Art Conventionally, a test chip is known in which a flow path is formed on a front surface and a back surface, and a hole that connects the front surface flow path and the back surface flow path is provided. For example, the microchip described in Patent Document 1 includes a channel formed on the front surface and the back surface, and a through hole that connects the channel on the front surface and the channel on the back surface. In the test using the microchip, the liquid reagent measured in the measuring unit and the plasma component measured in the sample measuring unit are mixed in one mixing unit. The mixed liquid mixture passes through the through hole and moves to another mixing portion. Also, the liquid reagent held in the liquid reagent holding part moves to the other mixing part through the same through hole as the through hole through which the mixed liquid has passed, and is mixed with the mixed liquid.

JP 2009-109467 A

  For example, when one reagent that moves in the flow path on the front surface and another reagent that moves in the flow path on the back surface merge through a through hole, the other reagent can pass through the path through which one reagent passes. There is sex. In this case, one reagent remaining on the path may react with another reagent, which may affect the measurement result.

  An object of the present invention is to provide a test chip that reduces the possibility that two reagents will react and affect the measurement result before flowing into the mixing section.

A test chip according to the present invention is formed on a first plate surface of a plate material, and a concave first reagent quantification unit for quantifying a first reagent, and a second plate surface opposite to the first plate surface in the plate material A second reagent quantification unit that is quantified in the second reagent, and the first reagent quantified in the first reagent quantification unit and the second reagent quantified in the second reagent quantification unit are mixed together Formed on the first plate surface, a first connection channel connecting the first reagent quantification unit and the mixing unit, and formed on the second plate surface, and the second reagent quantification. A second connection channel extending from the portion toward the mixing unit, and a hole for joining the second connection channel to the first connection channel, wherein the first connection channel is a concave first a first wall extending in the mixing portion side opposite to the surface of the mixing portion of the reagent quantitation section, be opposed to the first wall Is formed by the second wall, one end of the second wall side of the hole is characterized by being formed along the second wall.

  In this case, the first reagent quantified in the first reagent quantification unit moves along the first wall surface facing the first reagent quantification unit and flows into the mixing unit. Moreover, since the one end part of the hole is formed along the second wall surface side, when the second reagent merges from the second connection channel to the first connection channel via the hole, the second wall surface And flows into the mixing section. For this reason, compared with the case where the path | route where the 2nd reagent flows immediately after joining a 1st connection flow path through a hole overlaps the path | route where a 1st reagent flows, the path | route and 2nd reagent which a 1st reagent flow are The area where the flowing path overlaps can be shortened. Therefore, the area where the first reagent and the second reagent can react before flowing into the mixing section can be shortened, and the liquid after the first reagent and the second reagent react with each other flows into the mixing section, and the inspection is performed. The possibility of affecting the results can be reduced.

  The test chip is connected to the first reagent quantification unit and the reagent guide unit for guiding the first reagent to the first reagent quantification unit, and is located on the first reagent quantification unit side from the hole, A reagent quantification surface connecting an end of the first reagent quantification unit on the side of the mixing unit and an end of the first reagent quantification unit opposite to the side of the mixing unit is parallel to the reagent quantification surface and the mixing unit A reagent channel wall surface inclined toward the first reagent guide part from the reagent virtual surface extending to the side, and is pulled in a direction perpendicular to the reagent channel wall surface from the end of the reagent channel wall on the mixing unit side The virtual line may intersect the first wall surface.

  The end of the inspection chip that faces the one end may be along the first wall surface.

  The test chip is connected to the hole in the second connection channel and receives the second reagent quantified in the second reagent quantification unit; and the reagent receiver in the second connection channel. A third wall surface that is inclined toward the reagent receiving portion as it goes to the mixing portion, and an end portion that faces the one end portion in the hole portion may be along the third wall surface. .

  The test chip forms an inlet through which the second reagent flows into the reagent receiving part in the second connection flow path, and includes an inflow wall part facing the reagent receiving part, and the third wall surface is You may connect to the edge part on the opposite side of the said inflow port in an inflow wall part.

In the inspection chip, the first wall surface is disposed closer to the mixing unit than the hole portion, has a fourth wall surface including an end portion on the mixing unit side, and the second wall surface is more than the hole portion than the hole portion. It has a fifth wall surface disposed on the mixing portion side and including an end portion on the mixing portion side, and the first connection channel is located between the central portions of the fourth wall surface and the fifth wall surface. A reagent inflow port located between the first region and the end of the fourth wall surface and the fifth wall surface on the side of the mixing unit, and through which the first reagent and the second reagent flow into the mixing unit; A width between the fourth wall surface and the fifth wall surface in the first region is greater than a width between the fourth wall surface and the fifth wall surface in the second region. May be larger.

In the inspection chip, the first wall surface is disposed closer to the mixing unit than the hole portion, has a fourth wall surface including an end portion on the mixing unit side, and the second wall surface is more than the hole portion than the hole portion. A fifth wall surface disposed on the mixing portion side and including an end portion on the mixing portion side and a sixth wall surface connected to the hole portion side with respect to the fifth wall surface, the first connection flow path, A third region located between an end of the fourth wall surface on the hole side and the sixth wall surface, and a fourth region located between the respective center portions of the fourth wall surface and the fifth wall surface. And the width between the fourth wall surface and the fifth wall surface in the fourth region may be larger than the width between the fourth wall surface and the sixth wall surface in the third region. .

  The inspection chip is provided on the side of the mixing unit with respect to the hole or the hole, and the width of the channel in the depth direction perpendicular to the direction in which the first connection channel extends is narrowed toward the mixing unit. An inclined surface is provided, and the width in the depth direction on the mixing unit side of the inclined surface of the first connection channel is that of the first connection channel on the first reagent quantitative unit side of the hole. It may be larger than the width in the depth direction.

It is a figure which shows the structure of the test | inspection system 3 containing the test | inspection apparatus 1 and the control apparatus 90. FIG. It is a front view of the test | inspection chip 2. FIG. It is a rear view of the test | inspection chip 2. FIG. It is the II sectional view taken on the line in FIG. It is a flowchart of a centrifugation process. It is a state transition diagram of the test | inspection chip 2 in a centrifugation process. FIG. 7 is a state transition diagram of the test chip 2 continued from FIG. 6. FIG. 8 is a state transition diagram of the test chip 2 continued from FIG. 7. FIG. 4 is a front view of the test chip 2 showing a state in which the first reagent 18 flows from the reagent quantitative unit 134A to the mixing unit 80. 4 is a front view of the test chip 2 showing a state in which the second reagent 19 flows to the mixing unit 80. FIG. FIG. 6 is a front view of the inspection chip 2 showing a path through which the first reagent 18 and the second reagent 19 flow into the mixing unit 80. 2 is a front view of an inspection chip 200. FIG. 4 is a rear view of the inspection chip 200. FIG.

  DESCRIPTION OF EMBODIMENTS Embodiments embodying the present invention will be described with reference to the drawings. FIG. 1 shows a plane of the inspection apparatus 1 constituting the inspection system 3 and functional blocks inside the control apparatus 90.

<1. Schematic structure of inspection system 3>
A schematic structure of the inspection system 3 will be described with reference to FIG. The inspection system 3 of the present embodiment includes an inspection chip 2 that can store a sample and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2. When the inspection device 1 rotates the inspection chip 2 around the vertical axis A <b> 1 separated from the inspection chip 2, centrifugal force acts on the inspection chip 2. When the inspection apparatus 1 rotates the inspection chip 2 around the horizontal axis A2, the centrifugal direction, which is the direction of the centrifugal force acting on the inspection chip 2, is switched. In addition, since the inspection system 3 and the inspection apparatus 1 of this embodiment have a known structure as described in JP 2012-78107 A, in the following description, an outline of the structure of the inspection apparatus 1 will be described. To do.

<2. Structure of the inspection apparatus 1>
The structure of the inspection apparatus 1 will be described with reference to FIG. In the following description, the upper side, the lower side, the right side, the left side, the front side of the paper surface, and the rear side of the paper surface in FIG. . In the present embodiment, the direction of the vertical axis A1 is the vertical direction of the inspection apparatus 1, and the direction of the horizontal axis A2 is the direction of the speed when the inspection chip 2 is rotated about the vertical axis A1. FIG. 1 shows a state where the top plate of the upper housing 30 of the inspection apparatus 1 is removed.

  As shown in FIG. 1, the inspection apparatus 1 includes an upper housing 30, a lower housing 31, an upper plate 32, a turntable 33, an angle changing mechanism 34, and a control device 90. The turntable 33 is a disk rotatably provided on the upper side of an upper plate 32 described later. The inspection chip 2 is held above the turntable 33. The angle changing mechanism 34 is a drive mechanism provided on the turntable 33. The angle changing mechanism 34 rotates the inspection chip 2 around the horizontal axis A2. The upper housing 30 is fixed to an upper plate 32 described later, and a measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside. The control device 90 is a controller that controls various processes of the inspection device 1.

  A schematic structure of the lower housing 31 will be described. The lower housing 31 has a box-shaped frame structure in which frame members are combined. An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 31. A drive mechanism that rotates the turntable 33 around the vertical axis A1 is provided in the lower housing 31 as follows.

  A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side in the lower housing 31. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.

  The main shaft 57 is rotatably held by a support member (not shown) provided immediately below the upper plate 32. A pulley 38 is fixed to the main shaft 57 below the support member. A belt 39 is stretched over the pulley 37 and the pulley 38. When the main shaft motor 35 rotates the shaft 36, the driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39, and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

  A guide rail (not shown) extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31. A T-shaped plate (not shown) is movable in the vertical direction in the lower housing 31 along the guide rail.

  The aforementioned main shaft 57 is a cylindrical body having a hollow inside. An inner shaft (not shown) is a shaft that can move in the vertical direction inside the main shaft 57. The upper end portion of the inner shaft passes through the main shaft 57 and is connected to the rack gear 43. A bearing (not shown) is provided at the left end of the T-shaped plate. Inside the bearing, the lower end portion of the inner shaft is rotatably held.

  A stepping motor 51 for moving the T-shaped plate up and down is fixed in front of the T-shaped plate. The shaft 58 of the stepping motor 51 protrudes rearward, that is, downward in FIG. A disc-shaped cam plate (not shown) is fixed to the tip of the shaft 58. A cylindrical projection (not shown) is provided on the rear surface of the cam plate. The tip of the protrusion is inserted into a groove (not shown). The protrusion can slide in the groove. When the stepping motor 51 rotates the shaft 58, the protrusion moves up and down in conjunction with the rotation of the cam plate. At this time, the T-shaped plate moves up and down along the guide rail in conjunction with the protrusion inserted in the groove.

  The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 (not shown) fixed to the inner shaft is provided between the pair of L-shaped plates 60. The rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.

  On the front end side in the extending direction of each L-shaped plate 60, a horizontal support shaft 46 having a gear 45 is rotatably supported. The support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis (not shown) is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 is rotated about the support shaft 46.

  In the present embodiment, as the main shaft motor 35 rotationally drives the turntable 33, the inspection chip 2 rotates around the main shaft 57 that is a vertical axis, and a centrifugal force acts on the inspection chip 2. The rotation around the vertical axis A1 of the inspection chip 2 is referred to as revolution. On the other hand, as the stepping motor 51 moves the inner shaft up and down, the inspection chip 2 rotates about the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively. The rotation around the horizontal axis A2 of the inspection chip 2 is called autorotation.

  In a state where the T-shaped plate is lowered to the lowermost end of the movable range, the rack gear 43 is also lowered to the lowermost end of the movable range. At this time, the inspection chip 2 is in a steady state where the rotation angle is 0 degree. Further, in the state where the T-shaped plate is raised to the uppermost end of the movable range, the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the test | inspection chip 2 will be in the state rotated 180 degree | times centering on the horizontal axis line A2 from the steady state. That is, in this embodiment, the angle width that the test chip 2 can rotate is the rotation angle of 0 degrees to 180 degrees.

  The detailed structure of the upper housing 30 will be described. As shown in FIG. 1, the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.

  The measurement unit 7 provided inside the upper housing 30 includes a light source 71 that emits measurement light, and an optical sensor 72 that detects the measurement light emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. In the present embodiment, the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light. When the inspection chip 2 is at the measurement position, the measurement light connecting the light source 71 and the optical sensor 72 intersects the front surface and the rear surface of the inspection chip 2 substantially perpendicularly.

<3. Electrical configuration of control device 90>
The electrical configuration of the control device 90 will be described with reference to FIG. The control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for a user to input instructions to the control device 90, a hard disk device 95 for storing various data and programs, and a display 96 for displaying various information. As the control device 90, a personal computer may be used, or a dedicated control device may be used.

  Further, a revolution controller 97, a rotation controller 98, and a measurement controller 99 are connected to the CPU 91. The revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35. The rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51. The measurement controller 99 performs the optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the optical sensor 72 to the light source 71 and the optical sensor 72. The CPU 91 controls the revolution controller 97, the rotation controller 98, and the measurement controller 99.

<4. Structure of inspection chip 2>
With reference to FIG.2 and FIG.3, the detailed structure of the test | inspection chip 2 which concerns on this embodiment is demonstrated. In the following description, the upper, lower, left, right, front side, and back side of FIG. 2 are the upper, lower, left, right, front, and rear sides of the inspection chip 2, respectively. .

  As shown in FIGS. 2 and 3, the inspection chip 2 has a square shape when viewed from the front as an example, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness. As shown in FIG. 2, the front surface 201 of the plate member 20 is sealed with a sheet 291 made of a transparent synthetic resin thin plate. As shown in FIG. 3, the rear surface 202 opposite to the front surface 201 is sealed with a sheet 292 made of a transparent synthetic resin thin plate. As shown in FIGS. 2 and 3, a liquid flow path 25 is formed between the plate material 20 and the sheet 291 and between the plate material 20 and the sheet 292 so that the liquid sealed in the inspection chip 2 can flow. Has been. The liquid channel 25 is a recess formed at a predetermined depth on the front surface 201 side and the rear surface 202 side of the plate material 20, and extends in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate material 20. The sheets 291 and 292 seal the flow path forming surface of the plate material 20. The sheets 291 and 292 are not shown except for FIGS.

  The liquid channel 25 includes a sample quantitative channel 11, reagent quantitative channels 13, 15, a mixing unit 80, a measuring unit 81, and the like. The reagent fixed amount flow path 13 includes a first connection flow path 301. The reagent fixed amount flow path 15 includes a second connection flow path 331. As shown in FIG. 2, the mixing unit 80 is provided in the lower right portion of the front surface 201. The reagent fixed amount flow path 13 extends from the upper left part on the front surface 201 toward the mixing unit 80. The sample fixed amount flow path 11 extends from the upper right part on the front surface 201 toward the mixing part 80. As shown in FIG. 3, the reagent fixed amount flow path 15 extends from the upper left part on the rear surface 202 side toward the mixing unit 80. The mixing unit 80 is an area that includes a channel on the right side of an end 315 (described later) and an inlet 306 (described later) that is connected to a channel 117 (described later) and extends downward. The measurement unit 81 is a lower part of the mixing unit 80.

  A configuration common to the reagent quantitative channels 13 and 15 will be described. As shown in FIGS. 2 and 3, the reagent quantification channels 13 and 15 include the inlet 130, reagent holding unit 131, supply unit 132, reagent quantification unit 134, passage 137, guide unit 139, and surplus unit 136, respectively. including. The reagent holding part 131 is provided in the upper left part of the test chip 2. The reagent holding part 131 is a recess that opens upward. The injection port 130 penetrates the plate member 20 from the upper part of the reagent holding part 131 toward the upper side part 21 of the test chip 2. The inlet 130 is a part where the first reagent 18 or the second reagent 19 is injected into the reagent holding part 131. The reagent holding part 131 of the reagent fixed amount flow channel 13 is a part where the first reagent 18 injected from the injection port 130 of the reagent fixed amount flow channel 13 is stored. The reagent holding part 131 of the reagent fixed amount flow channel 15 is a part where the second reagent 19 injected from the injection port 130 of the reagent fixed amount flow channel 15 is stored. The second reagent 19 of the present embodiment is a reagent that is mixed after the first reagent 18 and a specimen 17A described later are mixed. In the following description, the first reagent 18 and the second reagent 19 are collectively referred to as “reagent 16” when not specified either.

  As shown in FIGS. 2 and 3, the supply unit 132 is a flow channel that extends downward from the upper right portion of the reagent holding unit 131. The lower end part of the supply part 132 is connected to the guide part 139 which is a passage having a narrow channel. A reagent quantitative unit 134 is provided below the guide unit 139. The guide unit 139 guides the reagent 16 to the reagent quantitative unit 134. The reagent quantification part 134 is a part where the reagent 16 is quantified, and is a concave part recessed in the lower left.

  The reagent quantification unit 134 is connected to the mixing unit 80 via the first connection channel 301 and is connected to the surplus unit 136 via the passage 137. The end on the mixing unit 80 side of the reagent quantitative unit 134 is referred to as a first end 141. The end of the reagent quantification unit 134 opposite to the mixing unit 80 is referred to as a second end 142. That is, the passage 137 extends from the second end portion 142 toward the surplus portion 136. A surface connecting the first end portion 141 and the second end portion 142 is a reagent fixed amount surface 146. The reagent quantification surface 146 is a virtual surface that is the position of the upper surface of the reagent 16 when the reagent 16 is quantified by the reagent quantification unit 134. Therefore, the volume of the liquid channel 25 below the reagent quantification surface 146 is the quantification amount in the reagent quantification unit 134.

  A passage 137 extends obliquely downward to the left from the upper part of the reagent fixed amount unit 134. The passage 137 is a channel through which the reagent 16 overflowing from the reagent quantitative unit 134 moves. A surplus part 136 is provided at the lower left of the reagent quantitative unit 134. The surplus portion 136 is a portion in which the reagent 16 that has moved through the passage 137 is accommodated, and is a recess provided in the downward direction and the right direction from the lower end of the passage 137.

  The first connection channel 301 will be described. In the following description, the reagent quantitative unit 134 of the reagent quantitative channel 13 is referred to as a reagent quantitative unit 134A, and the reagent quantitative unit 134 of the reagent quantitative channel 15 is referred to as a reagent quantitative unit 134B. The first connection channel 301 is a channel that is formed on the front surface 201 and connects the reagent quantitative unit 134A and the mixing unit 80. The first end portion 141 of the reagent quantitative unit 134A is a place where the reagent quantitative unit 134A and the first connection channel 301 are connected. The first connection channel 301 extends obliquely upward to the right from the first end 141 of the reagent quantitative unit 134A, extends downward from the right end, and further extends to the right from the lower end. The first connection channel 301 is formed by a first wall surface 302 and a second wall surface 303. The first wall surface 302 is a wall surface facing the reagent quantitative unit 134A and extending toward the mixing unit 80 side.

  The first wall surface 302 extends from the lower end of the guide portion 139 to a right end portion 314 that forms an inflow port 306 described later. More specifically, the first wall surface 302 includes wall surfaces 302A, 302B, 302C, 302D, 302E, and 302F. The wall surface 302A extends obliquely upward to the right from the lower end of the guide portion 139. Wall surface 302B extends diagonally downward to the right from the right end of wall surface 302A. Wall surface 302C extends obliquely downward to the right from the right end of wall surface 302B. Wall surface 302D extends obliquely downward to the right from the lower end of wall surface 302C. The wall surface 302D is inclined to the right side from the wall surface 302C extending obliquely downward to the right. Wall surface 302E extends rightward from the lower end of wall surface 302D. The wall surface 302F extends obliquely upward to the right while being bent from the right end of the wall surface 302D. More specifically, the wall surface 302F extends upward from the right end portion of the wall surface 302D, bends slightly to the right at the bent portion 321 and extends obliquely upward to the right, and further bends to the right at the bent portion 322 and extends obliquely upward to the right. The wall surface 302F faces a wall surface 303C described later.

  The second wall surface 303 is a surface of the wall surface forming the first connection channel 301 that is connected to the first end portion 141 of the reagent quantitative unit 134A. The first wall surface 302 and the second wall surface 303 face each other. The second wall surface 303 extends from the first end 141 of the reagent quantitative unit 134A to the right end 313 that forms an inflow port 306 described later.

  Of the second wall surface 303, the wall surface connected to the first end portion 141 of the reagent quantitative unit 134 </ b> A is referred to as a reagent channel wall surface 308. That is, the reagent channel wall surface 308 is connected to the reagent quantitative unit 134A in the first connection channel 301 and forms a part of the first connection channel 301. The reagent channel wall surface 308 is inclined to the guide unit 139 side from a virtual surface 320 that extends the reagent metering surface 146 of the reagent metering channel 13 parallel to the reagent metering surface 146 to the right side that is the mixing unit 80 side. More specifically, the reagent channel wall surface 308 extends obliquely upward to the right from the first end portion 141, bends slightly upward at the bent portion 309, and extends obliquely upward to the right. A virtual line 311 drawn from the end portion 310 of the reagent channel wall surface 308 on the mixing unit 80 side in a direction perpendicular to the reagent channel wall surface 308 intersects the wall surface 302C of the first wall surface 302.

  The second wall surface 303 includes a wall surface 303A, a wall surface 303B, and a wall surface 303C at the bottom. The wall surface 303 </ b> A is a wall surface extending vertically to the right of the surplus portion 136 of the reagent fixed amount flow path 13. The wall surface 303B is a wall surface extending in the right direction from the lower end of the wall surface 303A. The right end portion of the wall surface 303 </ b> B is located on the lower left side of the mixing unit 80. The wall surface 303C is a wall surface extending obliquely upward to the right from the right end portion of the wall surface 303B. Wall surface 303C faces wall surface 302F.

  The inflow port 306 is formed by the right end portion 313 of the wall surface 303C and the right end portion 314 of the first wall surface 302 positioned above the right end portion 313. The inflow port 306 is located on the left side of the mixing unit 80 and is a part for allowing the reagent 16 to flow into the mixing unit 80.

  A confluence hole portion 351 is provided at the center in the left-right direction at the lower end of the first connection channel 301. The merge hole 351 is a hole that penetrates the plate member 20 in the front-rear direction and joins the second connection channel 331 to the first connection channel 301. Details of the merge hole 351 will be described later.

  The first connection channel 301 is provided with regions 501, 502, and 503 on the mixing unit 80 side from the merging hole 351. A region 501 is a region between the right end portion of the wall surface 302E and the wall surface 303B. A region 502 is a region between the central portion of the wall surface 302F and the central portion of the wall surface 303C. The region 502 is located closer to the mixing unit 80 than the region 501. The flow path width L2 in the region 502 is larger than the flow path width L1 in the region 501.

  A region 503 is a region where the inflow port 306 is formed. That is, the region 503 is a region between the right end 313 of the wall surface 303C and the right end 314 of the wall surface 302F. The region 503 is located closer to the mixing unit 80 than the region 502. The flow path width L2 in the region 502 is larger than the flow path width L3 in the region 503.

  The second connection channel 331 will be described. As shown in FIG. 3, the second connection channel 331 is a channel that is formed on the rear surface 202 and extends from the reagent quantitative unit 134 </ b> B toward the mixing unit 80 and connects the reagent quantitative unit 134 </ b> B and the mixing unit 80. The second connection channel 331 includes four reagent receiving portions 341, 342, 343, and 344. The reagent receiving parts 341 to 344 are parts that receive the second reagent 19 quantified by the reagent quantifying part 134B. The reagent receiving part 341 is a concave part that is located on the right side of the reagent fixed quantity part 134B and opens to the left. The reagent receiving part 342 is a recessed part that is located on the lower left side of the reagent receiving part 341 and opens upward. The reagent receiving portion 343 is a concave portion that is located to the right of the reagent receiving portion 342 and opens to the left. The reagent receiving part 344 is a recessed part that is located below the reagent receiving part 343 and opens upward.

  The second connection channel 331 extends obliquely upward to the right from the reagent determination unit 134B and is connected to the reagent receiver 341, and extends obliquely downward to the left from the reagent receiver 341 and is connected to the reagent receiver 342. The second connection channel 331 extends obliquely upward to the right from the reagent receiver 342 and is connected to the reagent receiver 343, and extends obliquely downward to the left from the reagent receiver 343 and is connected to the reagent receiver 344. The right end portion of the reagent receiving portion 344 is connected to the merge hole portion 351 and is connected to the first connection flow path 301 on the front surface 201 side.

  The lower end portion of the reagent receiving portion 343 is formed by a wall portion 345 extending obliquely downward to the left. The wall part 345 faces the reagent receiving part 344. The left end 346 of the wall 345 forms an inlet 347 through which the second reagent 19 flows from the reagent receiver 343 to the reagent receiver 344.

  A wall surface 349 is connected to an end portion 348 of the lower surface of the wall portion 345 opposite to the inlet 347. Wall surface 349 faces reagent receiver 344. The wall surface 349 is inclined downward toward the reagent receiving portion 344 side toward the right side that is the mixing portion 80 side. The wall surface 349 is along the wall surface 302D shown in FIG.

  The merging hole portion 351 and the peripheral structure of the merging hole portion 351 will be described. As shown in FIG. 2, the lower end portion 352 on the second wall surface 303 side in the merging hole portion 351 extends in the left-right direction along the wall surface 303 </ b> B of the second wall surface 303. An upper end portion 353 that faces the lower end portion 352 extends obliquely downward to the right along the wall surface 302D of the first wall surface 302. Further, as shown in FIG. 3, the upper end portion 353 is along the wall surface 349 of the second connection channel 331.

  As shown in FIG. 2, the left end portion 354 of the merge hole portion 351 extends downward from the left portion of the wall surface 302D. As shown in FIG. 4, the left end 354 is formed by the right end of the partition 361 that separates the first connection channel 301 and the second connection channel 331. As shown in FIG. 2, the right end portion 355 of the merge hole portion 351 extends downward from the right end portion of the wall surface 302D. As shown in FIG. 4, the right end 355 is formed by the left end of an inclined surface 505 described later.

  An inclined surface 505 is provided on the mixing portion 80 side of the merge hole portion 351. As shown in FIG. 4, the inclined surface 505 is formed to be inclined forward from the rear end of the inspection chip 2 toward the right side. For this reason, the inclined surface 505 narrows the width of the channel in the depth direction orthogonal to the direction in which the first connection channel 301 extends as it goes toward the mixing unit 80 side. Of the first connection channel 301, the right-side depth L 4 that is on the mixing unit 80 side from the inclined surface 505 is greater than the left-side width L 5 that is on the reagent determination unit 134 A side from the merging hole 351. large.

  The specimen quantification channel 11 will be described. As shown in FIG. 2, the sample fixed amount flow path 11 includes an injection port 110, a sample holding unit 111, a first supply unit 112, a first guide unit 113, a separation unit 124, a passage 125, a passage 127, and a first surplus portion. 126, the second supply unit 123, the sample determination unit 114, the passage 115, the passage 117, and the second surplus unit 116. The sample holding unit 111 is provided on the right side of the supply unit 132 of the reagent fixed amount flow channel 13. The sample holder 111 is a recess that opens upward. The injection port 110 penetrates the plate member 20 from the upper part of the specimen holding part 111 toward the upper side part 21 of the test chip 2. The injection port 110 is a part where the sample 17 is injected into the sample holding unit 111. The sample holding unit 111 is a part where the sample 17 injected from the injection port 110 is stored. The specimen 17 of the present embodiment is a liquid containing components such as blood, plasma, blood cells, bone marrow, urine, vaginal tissue, epithelial tissue, tumor, semen, saliva, or foodstuff. The first supply unit 112 is a flow path that extends downward from the upper right portion of the specimen holding unit 111. The lower end part of the 1st supply part 112 is connected with the 1st guide part 113 which is a channel | path with which the flow path was narrowly formed.

  A separation unit 124 is provided below the first guide unit 113. The first guide unit 113 guides the sample 17 to the separation unit 124. The separation unit 124 is a part where components contained in the specimen 17 are separated. The separation part 124 is a recess that opens upward and tilts diagonally downward to the right. The separation unit 124 centrifuges the specimen 17 into a component having a small specific gravity and a component having a large specific gravity by the action of centrifugal force. In the following description, as shown in FIG. 6C, a component having a small specific gravity of the sample 17 separated in the separation unit 124 is referred to as a sample 17A, and a component having a large specific gravity is referred to as a sample 17B.

  The connecting channel 120 extends obliquely upward to the right from the center in the vertical direction on the right side surface of the separation unit 124, and the upper end of the connecting channel 120 is connected to the upper end of the component holding unit 121. The component holding unit 121 is a storage unit that holds the sample 17A and a part of the sample 17B separated by the separation unit 124. Further, the width of the flow path of the connection flow path 120 is narrower than the width of the flow path of the passage 127 described later. Therefore, the specimen 17A flows out to the passage 127 before flowing into the connection channel 120. Therefore, the possibility that the sample 17A flows into the component holding unit 121 before the passage 127 can be reduced.

  From the upper part of the separation part 124, the passage 125 extends obliquely to the left and the passage 127 extends obliquely upward to the right. The passage 125 extends to the first surplus portion 126 provided on the lower left side of the separation portion 124. The first surplus portion 126 is a portion where the specimen 17 overflowing from the separation portion 124 is stored, and is a recess provided in the right direction and the downward direction from the lower end portion of the passage 125.

  The passage 127 is connected to the second supply unit 123. The second supply unit 123 is a flow path that extends downward from the upper right portion of the passage 127. The lower end of the 2nd supply part 123 is connected with the 2nd guide part 128 which is a channel | path with which the flow path was narrowly formed. Below the second guide unit 128, a sample quantitative unit 114 is provided. The second guide unit 128 guides the sample 17A to the sample determination unit 114. The sample quantification unit 114 is a part that quantifies the sample 17A, and is a recess that opens upward.

  The sample quantification unit 114 is connected to the mixing unit 80 through the passage 117 and is connected to the second surplus unit 116 through the passage 115. The end of the sample determination unit 114 on the mixing unit 80 side is referred to as a first sample end 118. The end of the sample determination unit 114 opposite to the mixing unit 80 is referred to as a second sample end 119. That is, the passage 115 extends from the second specimen end 119 to the second surplus portion 116. A surface connecting the first sample end portion 118 and the second sample end portion 119 is a sample determination surface 129. The sample quantification surface 129 is a virtual surface serving as the position of the upper surface of the sample 17A when the sample 17A is quantified by the sample quantification unit 114. Therefore, the volume of the liquid channel 25 below the sample quantification surface 129 is the quantification amount in the sample quantification unit 114.

  From the upper part of the sample determination unit 114, the passage 115 extends obliquely to the left and the passage 117 extends obliquely upward to the right. A second surplus part 116 is provided at the lower left of the sample quantification part 114. The passage 115 is connected to the second surplus portion 116. The second surplus part 116 is a part where the specimen 17A overflowing from the specimen quantification part 114 is stored. The second surplus portion 116 is a recess provided in the right direction from the lower end portion of the passage 115. The passage 117 is connected to the mixing unit 80. A wall surface provided on the mixing unit 80 side of the sample quantitative unit 114 and connected to the sample quantitative unit 114 is referred to as a sample flow channel wall surface 312.

  The mixing unit 80 extends downward on the right side of the end portion 315 and the inlet 306 on the mixing unit 80 side of the sample determination unit 114. The mixing unit 80 is connected to the sample quantifying unit 114 via the passage 117. The mixing unit 80 is connected to the reagent quantitative unit 134A via the first connection channel 301. The mixing unit 80 is connected to the reagent quantification unit 134B via the second connection channel 331. In the mixing unit 80, the sample 17A quantified in the sample quantification unit 114, the first reagent 18 quantified in the reagent quantification unit 134A, and the second reagent 19 quantified in the reagent quantification unit 134B are mixed. When optical measurement described later is performed, the measurement light is transmitted to the measurement unit 81 that forms the lower part of the mixing unit 80.

<5. Other structures of inspection chip 2>
As shown in FIG. 1, the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates around the support shaft 46. When the inspection chip 2 is in the steady state shown in FIGS. 2 and 3, the upper side 21 and the lower side 24 are orthogonal to the direction of gravity G, the right side 22 and the left side 23 are parallel to the direction of gravity G, and The left side portion 23 is disposed closer to the main shaft 57 than the right side portion 22. In a state where the inspection chip 2 in the steady state is arranged at the measurement position, the inspection apparatus 1 performs inspection by optical measurement by allowing the measurement light connecting the light source 71 and the optical sensor 72 to pass through the measurement unit 81.

<6. Example of inspection method>
An inspection method using the inspection apparatus 1 and the inspection chip 2 will be described. As shown in FIG. 2, the sample 17 is injected from the injection port 110 and placed in the sample holding unit 111. The first reagent 18 is injected from the inlet 130 of the reagent quantitative flow path 13 and is disposed in the reagent holding part 131 of the reagent quantitative flow path 13. As shown in FIG. 3, the second reagent 19 is injected from the inlet 130 of the reagent quantitative channel 15 and is arranged in the reagent holding part 131 of the reagent quantitative channel 15. The arrangement method of the first reagent 18, the second reagent 19, and the specimen 17 is not limited. For example, holes are opened at positions corresponding to the sample holding unit 111 and the reagent holding unit 131 in the sheets 291 and 292, and the user injects the sample 17, the first reagent 18, and the second reagent 19 from the holes, You may seal and seal. In addition, the first reagent 18 and the second reagent 19 may be arranged in advance in the reagent holding portions 131 of the reagent quantitative flow paths 13 and 15 and sealed with sheets 291 and 292, respectively. In this case, a hole may be opened in the sheet 291 at a position corresponding to the sample holding part 111 of the sample fixed amount flow path 11, and the user may inject the sample 17 from the hole, and further seal and seal.

  The user attaches the inspection chip 2 to a mounting holder (not shown) and inputs a processing start command from the operation unit 94. As a result, the CPU 91 executes the centrifugal process shown in FIG. 5 based on the control program stored in the ROM 93. The inspection apparatus 1 can inspect two inspection chips 2 at the same time. For convenience of explanation, a procedure for inspecting one inspection chip 2 will be described below. In the following description, the steady state of the inspection chip 2 shown in FIGS. 2 and 3 is referred to as a rotation angle of 0 degree, and the state rotated 90 degrees counterclockwise from the steady state is referred to as a rotation angle of 90 degrees. In the following description, when the CPU 91 rotates the inspection chip 2 from 0 degree to 90 degrees, the inspection chip 2 rotates counterclockwise as viewed from the front. Further, when the CPU 91 rotates the inspection chip 2 from 90 degrees to 90 degrees, the inspection chip 2 rotates clockwise as viewed from the front.

  As shown in FIG. 5, the CPU 91 reads the motor drive information stored in advance in the HDD 95, sets the drive information of the spindle motor 35 in the revolution controller 97, and sets the drive information of the stepping motor 51 in the rotation controller 98. (S1). At this time, the test chip 2 is in a steady state and has a rotation angle of 0 degree as shown in FIGS. Next, the CPU 91 shown in FIG. 1 controls the revolution controller 97 to start driving the spindle motor 35 (S2). As a result, the inspection chip 2 having a rotation angle of 0 degrees revolves. The spindle motor 35 increases the rotation speed of the turntable 33 to the speed V based on an instruction from the revolution controller 97. The speed V is, for example, 3000 rpm. When the turntable 33 is rotated at this speed V, a centrifugal force X of several hundred G acts on the inspection chip 2. The CPU 91 maintains the rotation speed of the spindle motor 35 at the speed V (S3). As shown in FIG. 6A, centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22. The reagent 16 moves from the reagent holding unit 131 to the supply unit 132 by the action of the centrifugal force X. The sample 17 moves from the sample holding unit 111 to the first supply unit 112. In the following description, the rotation speed of the turntable 33 is assumed to be constant at the speed V, but the value of the speed V may be changed during the centrifugal process.

  Next, the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 90 degrees as shown in FIG. 6B (S4). As a result, the centrifugal force X acts on the test chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the reagent 16 flows from the supply unit 132 to the reagent quantitative unit 134 via the guide unit 139. The excess reagent 16 in the reagent quantification unit 134 flows to the surplus unit 136 via the passage 137. The centrifugal force X acts in the direction perpendicular to the reagent fixed amount surface 146. Thereby, the reagent 16 for the capacity of the reagent quantification unit 134 is quantified. In addition, the specimen 17 flows from the first supply unit 112 to the separation unit 124 via the first guide unit 113. The excess specimen 17 in the separation unit 124 flows to the first surplus part 126 through the passage 125. Therefore, the sample 17 corresponding to the volume of the separation unit 124 remains in the separation unit 124. The capacity of the separation part 124 is the capacity of the liquid flow path 25 below the virtual surface 148 extending in the right direction from the end part 147 on the passage 125 side in the separation part 124 shown in FIG.

  The CPU 91 maintains the rotation speed of the spindle motor 35 at the speed V for a predetermined time (S5). Thereby, the centrifugal force X acts on the test chip 2 from the upper side portion 21 toward the lower side portion 24 for a predetermined time. Thereby, as shown in FIG. 6C, in the separation unit 124, the components of the sample 17 are separated into the sample 17A and the sample 17B. For example, when the specimen 17 is blood, blood cells having a large specific gravity accumulate on the side in which the centrifugal force X acts, and plasma having a small specific gravity accumulates on the side opposite to the direction in which the centrifugal force X acts. That is, the specimen 17B, which is a blood cell in blood, and the specimen 17A, which is plasma, are separated.

  Next, the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51, and as shown in FIG. 7D, rotates the inspection chip 2 up to a rotation angle of 0 degrees (S6). As a result, the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.

  The flow of the first reagent 18 in the process in which the posture of the test chip 2 changes from the state shown in FIG. 6C to the state shown in FIG. FIG. 9 shows the flow of the first reagent 18 when the centrifugal force X is applied in the direction perpendicular to the portion on the right side of the bent portion 309 in the reagent channel wall surface 308. At this time, the first reagent 18 flows toward the wall surface 302 </ b> C of the first wall surface 302 along the virtual line 311 by the action of the centrifugal force X. The first reagent 18 flows along the wall surface 302C, the wall surface 302D, and the wall surface 302E of the first wall surface 302. The first reagent 18 flows from the right end portion of the wall surface 302E to the wall surface 303C, and flows into the mixing unit 80 via the inflow port 306.

  When the posture of the test chip 2 changes to the state shown in FIG. 7D, the first reagent 18 moves to the mixing unit 80, and the second reagent 19 quantified in the reagent quantification unit 134B moves to the reagent receiving unit 341. It becomes a state. In addition, the specimen 17A moves to the second supply unit 123 through the passage 127. The sample 17A remaining in the separation unit 124 and a part of the sample 17B move to the component holding unit 121 via the connection channel 120.

  Next, the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 up to a rotation angle of 90 degrees as shown in FIG. 7E (S7). As a result, the centrifugal force X acts from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the specimen 17A flows from the second supply section 123 to the specimen quantification section 114 via the second guide section 128. The sample 17A remaining in the sample determination unit 114 flows to the second surplus unit 116 via the passage 115. Thereby, the sample 17A corresponding to the volume of the sample quantification unit 114 is quantified. Further, the second reagent 19 held in the reagent receiving part 341 moves to the reagent receiving part 342.

  Next, the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to the rotation angle of 0 degrees as shown in FIG. 7F (S8). As a result, the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22. Due to the action of the centrifugal force X, the sample 17A quantified in the sample quantification unit 114 flows into the mixing unit 80 via the passage 117. Further, the first reagent 18 and the specimen 17A are mixed by the action of the centrifugal force X, and the first mixed liquid 261 is generated. Further, the second reagent 19 moves from the reagent receiving part 342 to the reagent receiving part 343.

  Next, the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51, and as shown in FIG. 8G, rotates the inspection chip 2 to a rotation angle of 90 degrees (S9). As a result, the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24. In the process in which the posture of the test chip 2 changes to the state shown in FIG. 8G, when the centrifugal force X acts in the direction perpendicular to the wall portion 345, the second reagent 19 passes through the path 831 in FIG. As shown in FIG. 4, the reagent is moved from the reagent receiving part 343 to the reagent receiving part 344 via the inflow port 347. The second reagent 19 that has moved to the reagent receiving part 344 flows into the first connection channel 301 formed in the front surface 201 via the junction hole part 351. As shown in FIG. 2, since the lower end 352 of the merging hole 351 is along the wall surface 303B of the second wall surface 303, the second reagent 19 flows into the first connection channel 301 along the wall surface 303B. Although not shown in FIG. 8G, the second reagent 19 that has flowed into the first connection channel 301 via the merge hole 351 may spread over the wall surface 303B by the action of the centrifugal force X. .

  Next, the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 0 degrees as shown in FIG. 8H (S10). As a result, the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22. In the process of changing the posture of the test chip 2 to the state shown in FIG. 8H, when the centrifugal force X is applied in the direction perpendicular to the wall surface 303C as shown in FIG. 10, the second reagent 19 moves from the wall surface 303C. It moves to the mixing unit 80 via the inflow port 306 and merges with the first mixed liquid 261. As shown in FIG. 8H, the second mixed liquid 262 in which the first reagent 18, the second reagent 19, and the specimen 17A are mixed is generated by the action of the centrifugal force X.

  Next, the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 90 degrees as shown in FIG. 8I (S11). As a result, the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the second mixed liquid 262 moves to the measuring unit 81.

  Although not shown in FIG. 8, after S <b> 11 is executed, the CPU 91 controls the rotation controller 98 to drive the stepping motor 51. The CPU 91 rotates the inspection chip 2 until the rotation angle is 0 degree (S12). Further, the CPU 91 controls the revolution controller 97 to stop the rotation of the spindle motor 35 (S12). Therefore, the revolution of the inspection chip 2 is completed. Centrifugation is terminated.

  After execution of the centrifugal process, the CPU 91 controls the revolution controller 97 to rotate and move the inspection chip 2 to the angle of the measurement position. When the measurement controller 99 shown in FIG. 1 causes the light source 71 to emit light, the measurement light passes through the second mixed liquid 262 stored in the measurement unit 81. The CPU 91 performs optical measurement of the second liquid mixture 262 based on the change amount of the measurement light received by the optical sensor 72, and acquires measurement data. CPU91 calculates the measurement result of the 2nd liquid mixture 262 based on the acquired measurement data. The inspection result of the second mixed liquid 262 based on the measurement result is displayed on the display 96 shown in FIG. In addition, the measuring method of the 2nd liquid mixture 262 is not restricted to an optical measurement, Another method may be sufficient.

<7. Main actions and effects of this embodiment>
As described above, the measurement in the present embodiment is performed. FIG. 11 shows a path 841 in which the first reagent 18 quantified in the reagent quantification unit 134A moves to the mixing unit 80, and the second reagent 19 that has flowed into the first connection channel 301 through the merging hole 351. A route 842 that moves to 80 is indicated by a bold line. In the present embodiment, as shown in the path 841 of FIGS. 9 and 11, the first reagent 18 quantified in the reagent quantification unit 134A moves along the first wall surface 302 facing the reagent quantification unit 134A. , Flows into the mixing unit 80. Further, the lower end portion 352 of the merge hole portion 351 is formed along the wall surface 303 </ b> B of the second wall surface 303. For this reason, the second reagent 19 flows from the second connection channel 331 into the first connection channel 301 through the junction hole 351 as shown in the path 842 of FIG. 8 (G), FIG. 9 and FIG. When it does, it moves along the wall surface 303 </ b> B of the second wall surface 303 and flows into the mixing unit 80. For this reason, as shown in FIG. 11, the area where the path 841 through which the first reagent 18 flows and the path 842 through which the second reagent 19 flows are limited to a partial area 843 of the wall surface 303C. Therefore, the path of the first reagent 18 and the path of the second reagent 19 immediately after flowing into the first connection channel 301 through the merge hole 351 overlap the path of the first reagent 18. The region where the path of the second reagent 19 overlaps can be shortened. The case where the path through which the second reagent 19 immediately after flowing into the first connection channel 301 through the junction hole 351 overlaps the path through which the first reagent 18 flows is, for example, the lower end 352 of the junction hole 351. However, it is a case where it is not formed along the wall surface 303 </ b> B of the second wall surface 303. Therefore, the region where the first reagent 18 remaining in the path through which the first reagent 18 flows and the second reagent 19 flowing to the mixing unit 80 after the first reagent 18 can react can be shortened. Therefore, the possibility that the second reagent 19 that has reacted with the first reagent 18 remaining in the path of the first reagent 18 after flowing the first reagent 18 flows into the mixing unit 80 and affects the test result can be reduced.

  As shown in FIG. 9, the first reagent 18 quantified in the reagent quantification unit 134A flows along the imaginary line 311 when a centrifugal force is applied in a direction perpendicular to the reagent channel wall surface. Since the virtual line 311 intersects with the first wall surface 302, the first reagent 18 hits the first wall surface 302. Therefore, the test chip 2 can flow the first reagent 18 along the first wall surface 302 more reliably than when the virtual line 311 does not intersect the first wall surface 302. Therefore, the region where the first reagent 18 remaining in the path through which the first reagent 18 flows and the second reagent 19 can react can be shortened more reliably. Therefore, the possibility that the second reagent 19 that has reacted with the first reagent 18 remaining in the path of the first reagent 18 after flowing the first reagent 18 flows into the mixing unit 80 and affects the test result can be reduced.

  If the upper end 353 of the merging hole 351 is not along the first wall surface 302, the first reagent 18 moving along the first wall 302 moves along the upper wall 353 and moves to the second wall 303. There is a possibility of approaching the side or contacting the second wall surface 303. Therefore, the second reagent 19 moving along the second wall surface 303 and the first reagent 18 may react. In the present embodiment, since the upper end portion 353 is along the first wall surface 302, even when the first reagent 18 moving along the first wall surface 302 travels along the upper end portion 353, the first reagent 18 is the first reagent 18. It moves along the wall surface 302. Therefore, compared with the case where the upper end part 353 is not along the 1st wall surface 302, possibility that the 1st reagent 18 will contact the 2nd wall surface 303 can be reduced. Therefore, the possibility that the second reagent 19 after reacting with the first reagent 18 flows into the mixing unit 80 and affects the inspection result can be further reduced.

  Further, as in the inspection chip 200 shown in FIGS. 12 and 13, a merging hole portion 800 is provided at a position different from the merging hole portion 351 of the inspection chip 2 shown in FIGS. 2 and 3. It is assumed that 301 and the second connection flow path 331 are connected. As shown in FIG. 12, the lower end portion 801 of the merging hole portion 800 is along the wall surface 303 </ b> B of the second wall surface 303. As shown in FIG. 13, the upper end portion 802 of the merge hole portion 800 is along the lower surface of the wall portion 345 extending obliquely downward to the left. Therefore, the upper end portion 802 is inclined obliquely downward to the left.

  In this case, as in the path 851 shown in FIGS. 12 and 13, the first reagent 18 moving along the first wall surface 302 travels along the upper end 802 and moves toward the second connection channel 331, and the wall 345. In some cases, the second connection flow path 331 may flow backward along. As a result, the first reagent 18 may fall to the reagent receiving part 344 in the second connection channel 331. In particular, for example, after the first mixed liquid 261 is generated, the revolution is stopped before the second reagent 19 is mixed, and so-called blind measurement is performed in which optical measurement is performed on the first mixed liquid 261. In this case, the centrifugal force X does not act on the inspection chip 2 and only the gravity G acts. The upper end part 802 and the wall part 345 are inclined obliquely downward to the left. When the blind measurement is performed, the gravity G acts in the downward direction of the test chip 2, so that the first reagent 18 remaining on the first wall surface 302 easily moves along the path 851. For example, when the first reagent 18 and the specimen 17A are mixed in the mixing unit 80, the gravity G is changed when the revolution and the stop of the revolution are switched and agitated. Only may work. Even in this case, the first reagent 18 remaining on the first wall surface 302 is likely to move along the path 851. The first reagent 18 that has moved along the path 851 easily falls from the right part of the wall part 345 to the reagent receiving part 344 due to the action of gravity G. For this reason, for example, in the second connection flow path 331, there is a possibility that the second reagent 19 held in the reagent receiving part 344 and the dropped first reagent 18 react.

  Further, as shown in FIG. 9, the first reagent 18 flows into the mixing unit 80 along the first wall surface 302. If the inclination of the wall surface 302 </ b> C is closer to the vertical direction than the state shown in FIG. 2, the first reagent 18 tends to remain on the wall surface 302 </ b> C guided through the first wall surface 302 to the mixing unit 80. After the first reagent 18 flows into the mixing unit 80 as shown in FIG. 7D, the test chip 2 is rotated to the rotation angle 90 as shown in FIG. It acts on the lower side portion 24. At this time, due to the action of the centrifugal force X, the first reagent 18 remaining on the wall surface 302C moves along the path 851 shown in FIGS. 12 and 13, and further falls to the reagent receiving portion 344, and the second reagent. 19 may react.

  In the present embodiment, as shown in FIG. 3, the wall surface 349 of the second connection channel 331 is inclined toward the reagent receiving part 344 toward the mixing part 80, and the upper end part 353 of the confluence hole part 351 is , Along the wall surface 349. For this reason, even when the first reagent 18 travels along the upper end 353 of the merging hole portion 351, the first reagent 18 is likely to move toward the mixing portion 80, and thus it is difficult for the first reagent 18 to flow back through the second connection channel 331. Therefore, the possibility that the first reagent 18 flows backward to the second connection channel 331 as shown by the path 851 in FIG. 13 and contacts the second reagent 19 on the second connection channel 331 side can be reduced. Therefore, the possibility that the second reagent 19 after reacting with the first reagent 18 flows into the mixing unit 80 and affects the inspection result can be further reduced.

  Further, as shown in FIG. 3, the wall surface 349 is connected to an end 348 opposite to the inflow port 347 on the lower surface of the wall portion 345, and a merge hole portion 351 is formed on the wall surface 349 side. Therefore, there is another surface between the wall portion 345 and the wall surface 349, and the wall surface 349 and the merge hole portion 351 are merged compared to the case where the wall portion 349 and the merge hole portion 351 are on the left side that is the mixing portion 80 side from the position shown in FIG. The position of the hole 351 is close to the inflow port 347. Therefore, the second reagent 19 flowing into the reagent receiving part 344 from the inflow port 347 flows out from the joining hole part 351 earlier along the path 831 in FIG. Therefore, the liquid level of the second reagent 19 flowing into the reagent receiving part 344 can be lowered more quickly. Therefore, the possibility that the second reagent 19 that has flowed into the reagent receiving part 344 flows backward through the inlet 347 can be reduced. Therefore, when the second reagent 19 flows backward, the amount of the second reagent 19 flowing into the mixing unit 80 is reduced, and the possibility of affecting the test result can be reduced.

  As shown in FIG. 2, in the first connection channel 301, the channel width L <b> 2 in the region 502 is larger than the channel width L <b> 3 in the region 503. For this reason, when the reagent 16 or the sample 17 once flowing into the mixing unit 80 tries to flow backward toward the region 502, surface tension is generated in the region 503, and the reagent 16 or the sample 17 becomes difficult to move. Therefore, the possibility that the reagent 16 or the sample 17 that has flowed into the mixing unit 80 flows back to the region 502 can be reduced, and the possibility that the second mixed liquid 262 in the mixing unit 80 is reduced to affect the measurement result can be reduced.

  The flow path width L2 in the region 502 is larger than the flow path width L1 in the region 501. For this reason, for example, a larger amount of the second reagent 19 than the second reagent 19 shown in FIG. 8G flows into the first connection channel 301 via the merge hole 351 and moves toward the region 502. In this case, surface tension is generated in the region 501, and the second reagent 19 becomes difficult to move. Therefore, it is possible to reduce the possibility that the second reagent 19 that has flowed into the first connection channel 301 via the merge hole portion 351 flows to the mixing portion 80 side due to the momentum that has flowed from the merge hole portion 351. Therefore, for example, when the second reagent 19 flows into the mixing unit 80 before the specimen 17 and the first reagent 18 are uniformly mixed in the mixing unit 80, the second reagent 19 flows into the mixing unit 80 by mistake. The possibility can be reduced, and the possibility of affecting the measurement result can be reduced.

  As shown in FIG. 4, in the first connection channel 301, the width L4 in the depth direction on the mixing unit 80 side from the inclined surface 505 is in the depth direction on the reagent quantitative unit 134 </ b> A side from the merging hole portion 351. It is larger than the width L5. For this reason, compared with the case where the width L4 is equal to or less than the width L5, the first reagent 18 that has flowed through the first connection channel 301 is less likely to hit the inclined surface 505. Therefore, the possibility that the first reagent 18 spreads by hitting the inclined surface 505 can be reduced. Therefore, when the 2nd reagent 19 goes to the mixing part 80 along the inclined surface 505, possibility that it will react with the 1st reagent 18 remaining on the inclined surface 505 can be reduced. Therefore, it is possible to further reduce the possibility that the second reagent 19 that has reacted with the first reagent 18 remaining on the inclined surface 505 flows into the mixing unit 80 and affects the inspection result.

  In the above embodiment, the front surface 201 is an example of the “first plate surface” in the present invention. The rear surface 202 is an example of the “second plate surface” in the present invention. The reagent quantification unit 134A is an example of the “first reagent quantification unit” in the present invention. The reagent quantification unit 134B is an example of the “second reagent quantification unit” in the present invention. The merge hole 351 is an example of the “hole” in the present invention. The lower end 352 is an example of the “one end” in the present invention. The upper end 353 is an example of the “end facing the one end” in the present invention. The guide part 139 of the reagent fixed amount flow channel 13 is an example of the “reagent guide part” of the present invention. The reagent receiver 344 is an example of the “reagent receiver” in the present invention. The wall surface 349 is an example of the “third wall surface” in the present invention. The wall portion 345 is an example of the “inflow wall portion” in the present invention. The inlet 347 is an example of the “inlet” in the present invention. The inlet 306 is an example of the “reagent inlet” in the present invention. The region 501 is an example of the “third region” in the present invention. The area 502 is an example of the “first area” and the “fourth area” in the present invention. The region 503 is an example of the “second region” in the present invention.

  In addition, this invention is not limited to said embodiment, A various change is possible. For example, the second reagent 19 has flowed into the mixing unit 80 after the first reagent 18, but is not limited thereto. The inspection chip 2 may be formed such that the first reagent 18 and the second reagent 19 flow into the mixing unit 80 at the same timing. For example, by forming a plurality of reagent receiving parts that receive the first reagent 18 in a path upstream from the reagent quantifying part 134A and delaying the timing at which the first reagent 18 flows into the reagent quantifying part 134A, The two reagents 19 may flow into the mixing unit 80 at the same timing. Even in this case, since the region where the path 841 through which the first reagent 18 flows and the path 842 through which the second reagent 19 flows is limited to a partial region 843 of the wall surface 303C, the first reagent 18 and the second reagent 19 The first reagent 18 and the second reagent 19 flow into the mixing unit 80 before the reaction of the first reagent 18 is completed. Therefore, the possibility that the reagent after the reaction of the first reagent 18 and the second reagent 19 flows into the mixing unit 80 and affects the test result can be reduced.

  Further, the merge hole portion 351 passes through a wall portion between the first connection channel 301 and the second connection channel 331 in order to merge the first connection channel 301 and the second connection channel 331. What is necessary is not to penetrate the plate member 20 in the front-rear direction. In this case, the inclined surface that narrows the width of the flow path in the depth direction is provided at the position of the merging hole 351, more specifically, the inclined surface is provided on the rear side of the merging hole 351 as it goes toward the mixing unit 80 side. Also good. In this case, since the rear portion of the merge hole portion 351 is formed with an inclined surface, the merge hole portion does not penetrate the plate member 20 in the front-rear direction.

  Further, the inclined surface 505 may not be provided. For example, the right end portion 355 of the merge hole portion 351 may not be formed by the left end of the inclined surface 505 but may be formed by a wall portion in the front-rear direction. Further, the width L4 of the first connection channel 301 shown in FIG. 3 may be equal to or smaller than the width L1 of the flow path in the region 501. The flow path width L2 in the region 502 may be equal to or smaller than the flow path width L3 in the region 503. In addition, the measurement unit 81 is a lower part of the mixing unit 80, but may be provided separately from the mixing unit 80.

  Further, another surface may exist between the wall portion 345 and the wall surface 349 shown in FIG. 3, and the wall surface 349 and the merge hole portion 351 may be on the left side that is closer to the mixing portion 80 than the position shown in FIG. . If the first reagent 18 does not contact the wall surface 303 </ b> B in the process in which the first reagent 18 shown in FIG. 9 flows from the first connection channel 301 to the mixing unit 80, the upper end 353 is moved downward from the first wall surface 302. May be along the first wall surface 302 in a state of being separated from each other or not parallel to the first wall surface 302. In addition, the upper end portion 353 of the merge hole portion 351 may not be along the first wall surface 302. Moreover, the lower end part 352 of the merging hole part 351 should just follow the 2nd wall surface 303, for example, the merging hole part 800 shown in FIG.12 and FIG.13 may be a "hole" of this invention. Further, in the process in which the second reagent 19 shown in FIGS. 8 (G) and 8 (H) flows into the first connection channel 301 and the mixing unit 80, the second reagent 19 does not contact the first wall surface 302. The lower end 352 may be along the second wall surface 303 in a state of being separated upward from the second wall surface 303 or not being parallel to the second wall surface 303.

2,200 Test chip 17, 17A, 17B Specimen 18 First reagent 19 Second reagent 20 Plate material 80 Mixing unit 134, 134A, 134B Reagent quantification unit 139 Guide unit 146 Reagent quantification surface 201 Front surface 202 Rear surface 301 First connection channel 302 First wall surface 303 Second wall surface 306, 347 Inlet 308 Reagent channel wall surface 311 Virtual line 320 Virtual surface 331 Second connection channel 341, 342, 343, 344 Reagent receiving part 345 Wall part 351, 800 Merge hole part 352 801 Lower end 353, 802 Upper end 501, 502, 503 area

Claims (8)

A concave first reagent quantification part formed on the first plate surface of the plate material and quantified by the first reagent;
Formed on the second plate surface opposite to the first plate surface in the plate material, a second reagent quantification unit for quantifying the second reagent;
A mixing unit in which the first reagent quantified in the first reagent quantification unit and the second reagent quantified in the second reagent quantification unit are mixed;
A first connection channel formed on the first plate surface and connecting the first reagent fixed amount unit and the mixing unit;
A second connection channel formed on the second plate surface and extending from the second reagent quantification part to the mixing part side;
A hole for joining the second connection flow path to the first connection flow path,
The first connection channel includes a first wall surface facing the mixing unit side of the concave first reagent quantification unit and extending to the mixing unit side; a second wall surface facing the first wall surface; Formed by
One end of the hole on the second wall surface side is formed along the second wall surface. A reagent guide for guiding the first reagent to the first reagent quantification unit;
Connected to the first reagent quantification part, located on the first reagent quantification part side from the hole, and on the mixing part side in the first reagent quantification part and the mixing part side in the first reagent quantification part And a reagent flow path wall surface that is inclined to the first reagent guide part side from a reagent virtual surface that extends to the mixing part side in parallel with the reagent quantitative surface. ,
2. The test chip according to claim 1, wherein an imaginary line drawn from an end of the reagent channel wall surface on the mixing unit side in a direction perpendicular to the reagent channel wall surface intersects the first wall surface. .   The inspection chip according to claim 1, wherein an end of the hole facing the one end is along the first wall surface. A reagent receiver connected to the hole in the second connection channel and receiving the second reagent quantified in the second reagent quantification unit;
In the second connection flow path, facing the reagent receiving portion, the third wall surface inclined toward the reagent receiving portion side toward the mixing portion,
The inspection chip according to claim 1, wherein an end portion of the hole portion that faces the one end portion is along the third wall surface. Forming an inflow port through which the second reagent flows into the reagent receiving part in the second connection channel, and an inflow wall part facing the reagent receiving part,
The inspection chip according to claim 4, wherein the third wall surface is connected to an end portion of the inflow wall portion opposite to the inflow port. The first wall surface is
It is arranged on the mixing part side from the hole part, and has a fourth wall surface including an end part on the mixing part side,
The second wall surface is
It is arranged on the mixing unit side from the hole, and has a fifth wall surface including an end on the mixing unit side,
The first connection flow path is
A first region located between the respective center portions of the fourth wall surface and the fifth wall surface ;
A second region that is located between the ends of the fourth wall surface and the fifth wall surface on the side of the mixing unit and that forms a reagent inlet through which the first reagent and the second reagent flow into the mixing unit And
The width between the fourth wall surface and the fifth wall surface in the first region is larger than the width between the fourth wall surface and the fifth wall surface in the second region. The inspection chip according to any one of 1 to 5. The first wall surface is
It is arranged on the mixing part side from the hole part, and has a fourth wall surface including an end part on the mixing part side,
The second wall surface is
A fifth wall surface disposed on the mixing portion side from the hole portion and including an end portion on the mixing portion side and a sixth wall surface connected to the hole portion side with respect to the fifth wall surface;
The first connection flow path is
A third region located between an end of the fourth wall surface on the hole side and the sixth wall surface ;
A fourth region located between the center of each of the fourth wall surface and the fifth wall surface ,
The width between the fourth wall surface and the fifth wall surface in the fourth region is larger than the width between the fourth wall surface and the sixth wall surface in the third region. The inspection chip according to any one of 1 to 5 . Provided on the side of the mixing unit from the hole or the hole, and as it goes toward the mixing unit, an inclined surface that narrows the width of the channel in the depth direction perpendicular to the direction in which the first connection channel extends,
Of the first connection channel, the width in the depth direction closer to the mixing unit than the inclined surface is greater than the depth direction of the first connection channel closer to the first reagent quantitative unit than the hole. The inspection chip according to claim 1, wherein the inspection chip is larger than a width.

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