WO2014133126A1

WO2014133126A1 - Test chip

Info

Publication number: WO2014133126A1
Application number: PCT/JP2014/055042
Authority: WO
Inventors: 由美子大鹿; 千里吉村
Original assignee: ブラザー工業株式会社
Priority date: 2013-02-28
Filing date: 2014-02-28
Publication date: 2014-09-04
Also published as: JP2014167452A

Abstract

Provided is a test chip that allows a specimen or reagent, which is retained inside a liquid introduction means by capillary forces, to flow into a fluid circuit at an appropriate time. A specimen introduction port that accommodates capillaries inserted from an opening (301A) is provided to the test chip (2). A specimen holding part (111), which holds a specimen, is provided to the specimen introduction port, on the opposite side to the opening (301A). The specimen held by the specimen holding part (111) is able to flow into a specimen supply part (112), whereby the specimen is guided towards a measurement part (190), via a flow passage inlet (120). The flow passage inlet (120) is provided closer to the specimen introduction port than the specimen holding part (111) in the direction of the specimen holding part (111) from the specimen introduction port.

Description

Inspection chip

The present disclosure relates to a test chip for performing a chemical, medical, or biological test of a test object.

Conventionally, a test chip capable of inserting a liquid introducing means for holding a specimen or a reagent inside by a capillary force is known. For example, the microfluidic chip disclosed in Patent Document 1 has a housing portion for housing a capillary. An opening for inserting a capillary capable of taking a liquid sample into the housing is formed on the upper surface or the lower surface of the microfluidic chip. The container is connected to a fluid circuit formed in the microfluidic chip. When a centrifugal force is applied to the capillary housed in the housing portion, the liquid sample flows out of the capillary and is introduced into the fluid circuit.

JP 2009-14450 A

As described above, in a microchip into which a capillary can be inserted, an inlet of a fluid circuit may be provided on the downstream side in the direction of inserting the capillary with respect to the accommodating portion. In this case, when the user inserts the capillary into the container, a part of the specimen or reagent held in the capillary may flow into the inlet of the fluid circuit. That is, there is a possibility that the specimen or reagent flows into the fluid circuit at an unintended timing. In this case, for example, the specimen or reagent may not be accurately mixed with other liquids, and the inspection accuracy may deteriorate.

An object of the present disclosure is to provide a test chip in which a specimen or a reagent held by a capillary force inside a liquid introduction unit can flow into a fluid circuit at an appropriate timing.

The inspection chip according to one aspect of the present disclosure has an opening into which a liquid introduction unit that holds a specimen or a reagent inside by a capillary force can be inserted, and is a portion in which the liquid introduction unit inserted from the opening is accommodated. An introduction port, and a holding unit which is provided on the opposite side of the opening with respect to the introduction port, and is a portion where the sample or the reagent flowing out from the liquid introduction means accommodated in the introduction port is held; A fluid circuit that is a flow path for guiding the specimen or the reagent toward a site where the liquid containing the specimen or the reagent is measured, and the specimen or the reagent held in the holding section are A channel inlet that is an opening that flows into the fluid circuit, and the channel inlet is provided closer to the inlet than the holder in an injection direction that is a direction from the inlet to the holder. That features To.

The inspection chip according to the above aspect is provided with an introduction port for accommodating the liquid introduction means inserted from the opening. A holding part for holding a specimen or a reagent is provided on the side opposite to the opening with respect to the introduction port. The specimen or reagent held in the holding section can flow into the fluid circuit through which the specimen or reagent is guided toward the measurement site via the flow path inlet. The flow path inlet is provided closer to the introduction port than the holding part in the injection direction. Thereby, the specimen or reagent flowing out from the liquid introduction means accommodated in the introduction port is held in the holding unit before flowing into the flow path inlet. As a result, the specimen or reagent flowing out from the liquid introduction means can move from the holding unit to the flow path inlet at an appropriate timing and flow into the fluid circuit.

A tilted surface that is provided between the introduction port and the holding portion and that is tilted with respect to the injection direction may be provided.

The inspection chip has a first surface and a second surface opposite to the first surface, the introduction port is provided on the first surface side, the holding portion, the fluid circuit, and the flow path The inlet is provided on the second surface side, and the inclined surface is inclined in a direction from the first surface toward the second surface so that the first surface side and the second surface side communicate with each other. Also good.

A capillary part that is provided between the introduction port and the holding unit and that holds the sample or the reagent flowing out from the liquid introduction unit accommodated in the introduction port by capillary force may be provided. .

A taper that is provided at an end of the capillary portion on the inlet side and is inclined with respect to the injection direction may be provided.

The introduction port and the capillary section communicate with the injection direction, and a cross section of the capillary section in a direction orthogonal to the injection direction is smaller than a cross section of the liquid introduction means in a direction orthogonal to the injection direction. Also good.

Provided between an end of the capillary part on the holding part side and the holding part, the connecting part being a flow path for the sample or the reagent, the introduction port, the capillary part, and the connection The flow path communicates with the injection direction, and the cross section of the capillary section in the direction orthogonal to the injection direction is smaller than the cross section of the introduction port in the direction orthogonal to the injection direction, and the injection direction of the introduction port The cross section in the direction perpendicular to the cross section may be smaller than the cross section in the direction perpendicular to the injection direction of the connection channel.

The inlet side wall part which is a wall part extended toward the said flow path inlet from the said holding | maintenance part may be provided, and the said inclined surface may incline toward the said inlet side wall part.

The holding unit may hold the specimen or the reagent by capillary force.

A step that is provided closer to the holding portion than the end of the inclined surface on the second surface side and projects in the second surface in the opposite direction to the first surface, or the opposite direction in the second surface. You may provide the constriction part which is a taper which inclines toward.

The inspection chip has a first surface and a second surface facing the first surface, and a width of the flow path inlet in a direction in which the first surface and the second surface are opposed is a width of the holding portion. It may be smaller than the width of the first surface and the second surface in the facing direction.

The inspection chip has a first surface and a second surface opposite to the first surface, and includes an inlet side wall portion that is a wall portion extending from the holding portion toward the flow channel inlet, A virtual direction extending from the end of the injection direction in parallel with the first surface and the second surface and extending perpendicular to the injection direction is a virtual direction, and the virtual direction and the inlet Of the angles formed with the side wall portions, the angle formed between the end portion of the introduction port in the injection direction and the flow channel inlet may be 90 degrees or less.

It is a figure which shows the structure of the test | inspection system 3 containing the test | inspection apparatus 1 and the control apparatus 90. FIG. It is the perspective view seen from the upper right side of the test | inspection chip 2. FIG. It is a front view of the test | inspection chip 2. FIG. It is a rear view of the test | inspection chip 2. FIG. FIG. 5 is a cross-sectional view taken along the line II of FIG. 3 and FIG. 4. FIG. 3 is an enlarged view of the back surface of the inspection chip 2 into which capillaries 401 to 406 are inserted. It is a front view of the test | inspection chip 2 revolved in 90 autorotation angles. It is a front view of the test | inspection chip 2 revolved in the autorotation angle 0 degree after FIG. It is a front view of the test | inspection chip 2 revolved in 90 autorotation angles after FIG. It is a front view of the test | inspection chip 2 revolved in the autorotation angle 0 degree after FIG. It is a front view of the test | inspection chip 2 after measurement operation | movement.

Embodiments embodying the present disclosure will be described with reference to the drawings. FIG. 1 shows a plane of the inspection apparatus 1 constituting the inspection system 3 and functional blocks inside the control apparatus 90.

<1. Schematic structure of inspection system 3>
An embodiment of the present disclosure will be described. A schematic structure of the inspection system 3 will be described with reference to FIG. The inspection system 3 of the present embodiment includes an inspection chip 2 that can store a sample and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2. When the inspection device 1 rotates the inspection chip 2 around the vertical axis A 1 separated from the inspection chip 2, centrifugal force acts on the inspection chip 2. When the inspection apparatus 1 rotates the inspection chip 2 around the horizontal axis A2, the centrifugal direction, which is the direction of the centrifugal force acting on the inspection chip 2, is switched. Note that the inspection system 3 and the inspection apparatus 1 of the present embodiment have a known structure as described in JP 2012-78107 A, and therefore, in the following description, an outline of the structure of the inspection apparatus 1 will be described. To do.

<2. Structure of the inspection apparatus 1>
The structure of the inspection apparatus 1 will be described with reference to FIG. In the following description, the upper, lower, right, left, front side, and back side of FIG. 1 are defined as the front, rear, right, left, upper, and lower sides of the inspection apparatus 1, respectively. . In the present embodiment, the direction of the vertical axis A1 is the vertical direction of the inspection apparatus 1, and the direction of the horizontal axis A2 is the direction of the speed when the inspection chip 2 is rotated about the vertical axis A1. FIG. 1 shows a state where the top plate of the upper housing 30 of the inspection apparatus 1 is removed.

As shown in FIG. 1, the inspection apparatus 1 includes an upper housing 30, a lower housing 31, an upper plate 32, a turntable 33, an angle changing mechanism 34, and a control device 90. The turntable 33 is a disk rotatably provided on the upper side of an upper plate 32 described later. The inspection chip 2 is held above the turntable 33. The angle changing mechanism 34 is a drive mechanism provided on the turntable 33. The angle changing mechanism 34 rotates the inspection chip 2 around the horizontal axis A2. The upper housing 30 is fixed to an upper plate 32 described later, and a measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside. The control device 90 is a controller that controls various processes of the inspection device 1.

The schematic structure of the lower housing 31 will be described. The lower housing 31 has a box-shaped frame structure in which frame members are combined. An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 31. A drive mechanism that rotates the turntable 33 around the vertical axis A1 is provided in the lower housing 31 as follows.

A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side of the lower housing 31. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.

The main shaft 57 is rotatably held by a support member (not shown) provided immediately below the upper plate 32. A pulley 38 is fixed to the main shaft 57 below the support member. A belt 39 is stretched over the pulley 37 and the pulley 38. When the main shaft motor 35 rotates the shaft 36, the driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39, and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

A guide rail (not shown) extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31. A T-shaped plate (not shown) is movable in the vertical direction in the lower housing 31 along the guide rail.

The above-described main shaft 57 is a hollow cylindrical body. An inner shaft (not shown) is a shaft that can move in the vertical direction inside the main shaft 57. The upper end portion of the inner shaft passes through the main shaft 57 and is connected to the rack gear 43. A bearing (not shown) is provided at the left end of the T-shaped plate. Inside the bearing, the lower end portion of the inner shaft is rotatably held.

A stepping motor 51 for moving the T-shaped plate up and down is fixed in front of the T-shaped plate. The shaft 58 of the stepping motor 51 protrudes rearward, that is, downward in FIG. A disc-shaped cam plate (not shown) is fixed to the tip of the shaft 58. A cylindrical projection (not shown) is provided on the rear surface of the cam plate. The tip of the protrusion is inserted into a groove (not shown). The protrusion can slide in the groove. When the stepping motor 51 rotates the shaft 58, the protrusion moves up and down in conjunction with the rotation of the cam plate. At this time, the T-shaped plate moves up and down along the guide rail in conjunction with the protrusion inserted in the groove.

The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 (not shown) fixed to the inner shaft is provided between the pair of L-shaped plates 60. The rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.

A horizontal support shaft 46 having a gear 45 is rotatably supported at the distal end side in the extending direction of each L-shaped plate 60. The support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis (not shown) is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 rotates about the support shaft 46.

In this embodiment, as the main shaft motor 35 rotates and drives the turntable 33, the inspection chip 2 rotates around the main shaft 57, which is a vertical axis, and centrifugal force is applied to the inspection chip 2. The rotation around the vertical axis A1 of the inspection chip 2 is referred to as revolution. On the other hand, as the stepping motor 51 moves the inner shaft up and down, the inspection chip 2 rotates about the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively. The rotation around the horizontal axis A2 of the inspection chip 2 is called autorotation.

When the T plate is lowered to the lowermost end of the movable range, the rack gear 43 is also lowered to the lowermost end of the movable range. At this time, the inspection chip 2 is in a steady state where the rotation angle is 0 degree. Further, in the state where the T-shaped plate is raised to the uppermost end of the movable range, the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the test | inspection chip 2 will be in the state rotated 180 degree | times centering on the horizontal axis line A2 from the steady state. That is, in this embodiment, the angular width that the test chip 2 can rotate is the rotation angle of 0 degrees to 180 degrees.

The detailed structure of the upper housing 30 will be described. As shown in FIG. 1, the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.

The measurement unit 7 provided in the upper housing 30 includes a light source 71 that emits measurement light, and an optical sensor 72 that detects the measurement light emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. In the present embodiment, the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light. When the inspection chip 2 is at the measurement position, the measurement light connecting the light source 71 and the optical sensor 72 intersects the surface of the inspection chip 2 substantially perpendicularly.

<3. Electrical configuration of control device 90>
The electrical configuration of the control device 90 will be described with reference to FIG. The control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for a user to input instructions to the control device 90, a hard disk device 95 for storing various data and programs, and a display 96 for displaying various information. As the control device 90, a personal computer may be used, or a dedicated control device may be used.

The revolution controller 97, the rotation controller 98, and the measurement controller 99 are connected to the CPU 91. The revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35. The rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51. The measurement controller 99 performs optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the optical sensor 72 to the light source 71 and the optical sensor 72. The CPU 91 controls the revolution controller 97, the rotation controller 98 and the measurement controller 99.

<4. Structure of inspection chip 2>
The detailed structure of the test chip 2 according to the present embodiment will be described with reference to FIGS. In the following description, the upper, lower, lower left, upper right, lower right, and upper left in FIG. 2 are the upper, lower, left, right, front, and rear of the test chip 2, respectively.

As shown in FIG. 2, the inspection chip 2 has a square shape when viewed from the front as an example, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness. The front surface 25 of the plate member 20 is sealed with a sheet 27 made of a transparent synthetic resin thin plate. The rear surface 26 of the plate 20 is sealed with a sheet 28 made of a transparent synthetic resin thin plate. The

sheets

27 and 28 are not shown except for FIG.

As shown in FIG. 3, the test chip 2 of the present embodiment has a sample or reagent flow path formed on both the front surface 25 and the rear surface 26. Between the plate member 20 and the sheet 27, a liquid flow path 100 is formed in which the liquid sealed in the inspection chip 2 can flow. The liquid flow path 100 is a recess formed at a predetermined depth on the front surface 25 side of the plate material 20 and extends in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate material 20. As shown in FIG. 4, between the plate member 20 and the sheet 28, a liquid introduction part 300 for injecting a liquid into the inspection chip 2 and a liquid flow path 200 through which the liquid injected into the inspection chip 2 can flow. And are formed. The liquid introduction part 300 and the liquid flow path 200 are recesses formed at a predetermined depth on the rear surface 26 side of the plate material 20 and extend in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate material 20.

<4-1. Detailed Structure of Liquid Channel 100>
The detailed structure of the liquid channel 100 will be described with reference to FIG. The liquid flow channel 100 includes a sample quantitative flow channel 110, reagent

quantitative flow channels

130 and 150, a common flow channel 180, and a measurement unit 190. The sample quantitative channel 110 is on the left side of the test chip 2, the reagent quantitative channel 130 is on the right side of the sample quantitative channel 110, the reagent quantitative channel 150 is on the right side of the reagent quantitative channel 130, and the test chip 2. It is provided in the right part of. The measurement unit 190 is provided in the lower right part of the inspection chip 2. The common flow channel 180 is provided between the sample quantitative flow channel 110 and the reagent

quantitative flow channels

130 and 150 and the measurement unit 190.

The sample quantification channel 110 includes a sample holding unit 111, a sample supply unit 112, a sample guide unit 113, a sample quantification unit 114, a channel 115, a sample surplus unit 116, and a channel 117. A guide hole 311 that penetrates the plate member 20 in the front-rear direction is formed in the upper left portion of the specimen fixed amount flow channel 110. Below the guide hole 311, an inclined surface 312 that is a surface that communicates the rear surface 26 and the front surface 25 is provided. As will be described later, the specimen 61 can flow into the specimen quantitative channel 110 from the specimen inlet 301 shown in FIG. 4 by moving through the guide hole 311 along the inclined surface 312.

The end on the front surface 25 side of the inclined surface 312 is an end 312A. Below the end 312 A, a specimen holding unit 111 that is a recess opening upward is provided. The sample holding unit 111 is a part where the sample 61 that has flowed into the sample fixed amount flow channel 110 through the guide hole 311 is held. A wall portion extending in the upper right direction from the specimen holding portion 111 is an inlet side wall portion 121. A flow path inlet 120 that communicates with the sample supply unit 112 is provided at the upper right end of the inlet side wall 121. The channel inlet 120 is an opening through which the sample 61 held in the sample holding unit 111 flows into the sample supply unit 112. A step that protrudes forward from the front surface 25 is provided across the vertical direction of the flow path inlet 120. Therefore, the channel width in the front-rear direction of the channel inlet 120 is smaller than the channel width in the front-rear direction of the specimen holding unit 111.

The specimen supply unit 112 is a flow path that extends downward from the flow path inlet 120. The flow path inlet 120 is provided closer to the sample inlet 301 than the sample holder 111 in the first injection direction. The first injection direction is a direction in which the capillary 401 is inserted into the sample introduction port 301. In other words, the first injection direction is a direction from the sample introduction port 301 toward the sample holding unit 111, that is, a downward direction. The flow path inlet 120 of this embodiment is provided above the guide hole 311 and the inclined surface 312.

The lower end of the sample supply unit 112 is connected to a sample guide unit 113 having a narrow channel. Below the sample guide unit 113, a sample quantitative unit 114 is provided. The specimen quantification unit 114 is a part that quantifies the specimen 61, and is a recess that is recessed in the lower left. From the part where the sample guide unit 113 and the sample determination unit 114 communicate with each other, the passage 115 extends to the lower left and the passage 117 extends to the upper right. The passage 115 extends to the sample surplus part 116 provided below the sample determination unit 114. The specimen surplus part 116 is a part where the specimen 61 overflowing from the specimen quantification part 114 is stored, and is a concave part provided in the right direction from the lower end part of the passage 115. The upper right end of the passage 117 is connected to a common channel 180 described later.

The reagent fixed amount flow path 130 includes a reagent holding unit 131, a reagent supply unit 132, a reagent guide unit 133, a reagent fixed amount unit 134, a passage 135, a reagent surplus portion 136, and a passage 137. A guide hole 313 that penetrates the plate member 20 in the front-rear direction is formed in the upper left portion of the reagent fixed amount flow path 130. An inclined surface 314 that is a surface that communicates the rear surface 26 and the front surface 25 is provided below the guide hole 313. As will be described later, the first reagent 62 can flow into the reagent quantitative flow channel 130 from the reagent inlet 303 shown in FIG. 4 by moving through the guide hole 313 along the inclined surface 314.

The end on the front surface 25 side of the inclined surface 314 is an end 314A. Below the end 314A, there is provided a reagent holding part 131 which is a recess opening upward. The reagent holding part 131 is a part that holds the first reagent 62 that has flowed into the reagent fixed amount flow path 130 via the guide hole 313. A wall portion extending in the upper right direction from the reagent holding portion 131 is an inlet side wall portion 141. A flow path inlet 140 communicating with the reagent supply unit 132 is provided at the upper right end of the inlet side wall 141. The flow path inlet 140 is an opening through which the first reagent 62 held in the reagent holding unit 131 flows into the reagent supply unit 132. The reagent supply unit 132 is a channel extending downward from the channel inlet 140. The channel inlet 140 is provided closer to the reagent inlet 303 than the reagent holding part 131 in the second injection direction. The second injection direction is the direction in which the capillary 403 is inserted into the reagent introduction port 303, in other words, the direction from the reagent introduction port 303 toward the reagent holding unit 131, that is, the lower left direction. The channel inlet 140 of the present embodiment is provided above the guide hole 313 and the inclined surface 314.

The inlet side wall 141 has a smaller inclination angle with respect to the vertical direction of the inspection chip 2 than the above-described inlet side wall 121 and the inlet side wall 161 described later. As an example, the inclination angle of the inlet side wall 121 and the inlet side wall 161 with respect to the vertical direction is 30 degrees, while the inclination angle of the inlet side wall 141 with respect to the vertical direction is 10 degrees. The inclination angle with respect to the vertical direction of the inlet

side wall portions

221, 241, 261 described later shown in FIG.

The lower end of the reagent supply unit 132 is connected to a reagent guide unit 133 having a narrow channel. A reagent quantitative unit 134 is provided below the reagent guide unit 133. The reagent quantification unit 134 is a part that quantifies the first reagent 62, and is a recess that is recessed in the lower left direction. A passage 135 extends to the lower left and a passage 137 extends to the upper right from a portion where the reagent guide 133 and the reagent quantitative unit 134 communicate with each other. The passage 135 extends to the reagent surplus portion 136 provided below the reagent fixed amount portion 134. The reagent surplus part 136 is a part in which the first reagent 62 overflowing from the reagent quantitative part 134 is stored, and is a concave part provided in the right direction from the lower end part of the passage 135. The upper right end of the passage 137 is connected to a common channel 180 described later.

The reagent quantitative flow path 150 includes a reagent holding part 151, a reagent supply part 152, a reagent guide part 153, a reagent quantitative part 154, a passage 155, a reagent surplus part 156, and a passage 157. A guide hole 315 that penetrates the plate member 20 in the front-rear direction is formed in the upper left portion of the reagent fixed amount flow path 150. Under the guide hole 315, an inclined surface 316 that is a surface that connects the rear surface 26 and the front surface 25 is provided. As will be described later, the second reagent 63 can flow into the reagent fixed amount flow path 150 from the reagent introduction port 305 shown in FIG. 4 by moving through the guide hole 315 along the inclined surface 316.

The end on the front surface 25 side of the inclined surface 316 is an end 316A. A reagent holding portion 151 that is a recess opening upward is provided below the end portion 316A. The reagent holding unit 151 is a part that holds the second reagent 63 that has flowed into the reagent fixed amount flow path 150 through the guide hole 315. The wall portion extending from the reagent holding portion 151 in the upper right direction is the inlet side wall portion 161. A flow path inlet 160 communicating with the reagent supply unit 152 is provided at the upper right end of the inlet side wall 161. The channel inlet 160 is an opening through which the second reagent 63 held in the reagent holding unit 151 flows into the reagent supply unit 152. The reagent supply unit 152 is a channel that extends downward from the channel inlet 160. The channel inlet 160 is provided closer to the reagent inlet 305 than the reagent holding part 151 in the third injection direction. The third injection direction is a direction in which the capillary 405 is inserted into the reagent introduction port 305, in other words, a direction from the reagent introduction port 305 toward the reagent holding unit 151, that is, a downward direction. The channel inlet 160 of the present embodiment is provided above the guide hole 315 and the inclined surface 316.

A narrowed portion 165 is provided closer to the reagent holding portion 151 than the end portion 316A on the front surface 25 side of the inclined surface 316. As shown in FIG. 2, the narrowed portion 165 is a step that protrudes forward in the front surface 25. Due to this step, the flow path width in the front-rear direction of the portion where the narrowed portion 165 is provided is narrower than the portion where the narrowed portion 165 is not provided. The narrowed portion 165 is provided from the lower position 165A of the end portion 316A to the lower end portion 151A of the reagent holding portion 151. The narrowed portion 165 may be a taper inclined forward in the front surface 25.

The lower end of the reagent supply unit 152 is connected to a reagent guide unit 153 having a narrow channel. A reagent quantitative unit 154 is provided below the reagent guide unit 153. The reagent quantification unit 154 is a part that quantifies the second reagent 63, and is a recess that is recessed downward to the left. A passage 155 extends to the lower left and a passage 157 extends to the upper right from a portion where the reagent guide unit 153 and the reagent quantitative unit 154 communicate with each other. The passage 155 extends to a reagent surplus portion 156 provided below the reagent fixed amount portion 154. The reagent surplus part 156 is a part in which the second reagent 63 overflowing from the reagent quantitative part 154 is stored, and is a concave part provided in the right direction from the lower end part of the passage 155. The upper right end of the passage 157 is connected to a common channel 180 described later.

The common flow path 180 is a flow path that connects the

passages

117, 137, and 157 and the measurement unit 190. The common flow path 180 includes

guide walls

181, 182 and 183. The guide wall 181 is a wall portion provided on the right side of the passage 117 and extending in the lower right direction. The guide wall 182 is a wall portion provided on the right side of the passage 137 and extending in the lower right direction. The guide wall 183 is a wall provided on the right side of the passage 137 and extending downward to the measurement unit 190. The measurement unit 190 is a concave portion provided in the lower right portion of the common channel 180 and recessed downward. The measurement unit 190 is a part where the liquid mixture 64 shown in FIG. 11, which is a liquid containing the specimen 61, the first reagent 62, and the second reagent 63, is stored and measured. When optical measurement to be described later is performed, measurement light is transmitted through the measurement unit 190.

<4-2. Detailed Structure of Liquid Introducing Unit 300>
The detailed structure of the liquid introduction unit 300 will be described with reference to FIGS. FIG. 5 shows the capillaries 405 accommodated in the reagent introduction port 305 with phantom lines. FIG. 6 is an enlarged view of the upper portion of the rear surface 26 of the inspection chip 2 in which the capillaries 401 to 406 are accommodated. As shown in FIG. 4, the liquid introduction unit 300 includes

sample introduction ports

301 and 302 and

reagent introduction ports

303, 304, 305, and 306.

As shown in FIG. 6, the sample introduction port 301 is provided at the upper left part of the rear surface 26 and extends downward from the upper side portion 21 to the approximate center of the test chip 2 in the vertical direction. The upper end portion of the sample introduction port 301 is an opening 301 A formed in the upper side portion 21. The capillary 301 can be inserted into the opening 301A. The sample introduction port 301 is a part in which the capillary 401 inserted from the opening 301A is accommodated. The capillary 401 is a hollow tube made of, for example, glass that can take in the specimen 61, and holds the specimen 61 inside by capillary force.

The sample introduction port 302 is provided on the rear surface 26 on the right side of the sample introduction port 301 and extends downward from the upper side portion 21 to the approximate center of the test chip 2 in the vertical direction. An upper end portion of the sample introduction port 302 is an opening 302 A formed in the upper side portion 21. Through the opening 302A, the capillary 402 can be inserted. The sample introduction port 302 is a part in which the capillary 402 inserted from the opening 302A is accommodated. The capillary 402 is a hollow tube made of, for example, glass that can take in the specimen 81, and holds the specimen 81 inside by capillary force. As an example, the

specimens

61 and 81 are blood.

The reagent introduction port 303 is provided on the upper side of the substantially central portion of the rear surface 26 and extends from the upper side portion 21 to the lower left to the approximate center of the test chip 2 in the vertical direction. An upper end portion of the reagent introduction port 303 is an opening 303 A formed in the upper side portion 21. Through the opening 303A, the capillary 403 can be inserted. The reagent introduction port 303 is a part in which the capillary 403 inserted from the opening 303A is accommodated. The capillary 403 is a hollow tube made of, for example, glass that can take in the first reagent 62, and holds the first reagent 62 inside by capillary force. As an example, the first reagent 62 is a reagent for measuring glucose in blood.

The reagent introduction port 304 is provided on the rear surface 26 on the right side of the reagent introduction port 303, and extends downward from the upper side portion 21 to the approximate center of the test chip 2 in the vertical direction. The upper end portion of the reagent introduction port 304 is an opening 304 A formed in the upper side portion 21. The capillary 304 can be inserted into the opening 304A. The reagent introduction port 304 is a part in which the capillary 404 inserted from the opening 304A is accommodated. The capillary 404 is a hollow tube made of, for example, glass that can take in the first reagent 82, and holds the first reagent 82 inside by capillary force. As an example, the first reagent 82 is a reagent for measuring total cholesterol in blood.

The reagent introduction port 305 is provided at the upper right portion of the rear surface 26 and extends downward from the upper side portion 21 to the approximate center of the test chip 2 in the vertical direction. An upper end portion of the reagent introduction port 305 is an opening 305 A formed in the upper side portion 21. The capillary 405 can be inserted into the opening 305A. The reagent introduction port 305 is a part in which the capillary 405 inserted from the opening 305A is accommodated. The capillary 405 is a hollow tube made of, for example, glass that can take in the second reagent 63, and holds the second reagent 63 inside by capillary force. An example is a reagent for measuring glucose in blood.

The reagent inlet 306 is provided on the rear surface 26 on the right side of the reagent inlet 305 and is recessed downward from the upper side 21. An upper end portion of the reagent introduction port 306 is an opening 306 A formed in the upper side portion 21. The capillary 406 can be inserted into the opening 306A. The opening width that is the length in the left-right direction of the reagent introduction port 306 is larger than the opening width that is the length in the left-right direction of the

sample introduction ports

301 and 302 and the

reagent introduction ports

303, 304, and 305. The reagent introduction port 306 is a part in which the capillary 406 inserted from the opening 306A is accommodated. The capillary 406 is a hollow tube made of, for example, glass that can take in the second reagent 83, and holds the second reagent 83 inside by capillary force. As an example, the second reagent 83 is a reagent for measuring total cholesterol in blood.

As shown in FIG. 5, a guide hole 315 is provided from the lower end of the reagent introduction port 305 toward the front. The inclined surface 316 is a surface inclined downward on the front side that forms the lower surface of the reagent introduction port 305 and the inner lower surface that forms the guide hole 315. The inclined surface 316 connects the reagent introduction port 305 on the rear surface 26 side and the reagent quantitative flow path 150 on the front surface 25 side. That is, the inclined surface 316 is provided in the flow path between the reagent introduction port 305 and the reagent holding unit 151, and is inclined with respect to the downward direction that is the third injection direction.

As shown in FIGS. 3 and 4, a guide hole 311 is provided from the lower end of the sample introduction port 301 toward the front. The inclined surface 312 is a surface inclined downward on the front side that forms the lower surface of the sample introduction port 301 and the inner lower surface forming the guide hole 311. The inclined surface 312 connects the sample inlet 301 on the rear surface 26 side and the sample quantitative flow channel 110 on the front surface 25 side. That is, the inclined surface 312 is provided in the flow path between the sample introduction port 301 and the sample holding unit 111 and is inclined with respect to the downward direction that is the first injection direction.

The inclined surface 314 is a surface inclined downward in the front direction that forms the lower surface of the reagent inlet 303 and the inner lower surface forming the guide hole 313. The inclined surface 314 connects the reagent introduction port 303 on the rear surface 26 side and the reagent fixed amount flow channel 130 on the front surface 25 side. That is, the inclined surface 314 is provided in the flow path between the reagent introduction port 303 and the reagent holding part 131 and is inclined with respect to the lower right direction that is the second injection direction.

<4-3. Detailed Structure of Liquid Channel 200>
With reference to FIG. 4, the detailed structure of the liquid flow path 200 is demonstrated. The liquid flow path 200 includes a sample quantitative flow path 210, reagent

quantitative flow paths

230 and 250, a common flow path 280, and a measurement unit 290. The sample quantification channel 210 is provided on the left side of the test chip 2 and has a shape substantially corresponding to the sample quantification channel 110 shown in FIG. The reagent quantitative channel 230 is provided on the right side of the sample quantitative channel 110 and has a shape substantially corresponding to the reagent quantitative channel 130 shown in FIG. The reagent quantitative channel 250 is provided on the right side of the reagent quantitative channel 130 and on the right part of the test chip 2 and has a shape substantially corresponding to the reagent quantitative channel 150 shown in FIG. The measuring unit 290 is provided at the lower right portion of the inspection chip 2 and has a shape substantially corresponding to the measuring unit 190 shown in FIG. The common flow path 280 is provided between the sample quantitative flow path 210 and the reagent

quantitative flow paths

230 and 250 and the measurement unit 290, and has a shape substantially corresponding to the common flow path 180 shown in FIG. Have.

The sample quantification channel 210 includes a sample holding unit 211, a sample supply unit 212, a sample guide unit 213, a sample quantification unit 214, a channel 215, a sample surplus unit 216, and a channel 217. The lower end portion of the sample introduction port 302 is connected to the upper left end portion of the sample fixed amount flow path 210. Below the sample introduction port 302, an inclined surface 222, which is a wall surface inclined in the lower right direction, is provided. Below the inclined surface 222, a sample holder 211 that is a concave portion that opens upward is provided.

As will be described later, the specimen 81 can flow from the specimen introduction port 302 into the specimen quantitative channel 210 by moving to the lower right along the inclined surface 222. That is, the inclined surface 222 is provided between the sample introduction port 302 and the sample holder 211 and is inclined with respect to the downward direction, which is the fourth injection direction. The fourth injection direction is the direction in which the capillary 402 is inserted into the sample introduction port 302, in other words, the direction from the sample introduction port 302 toward the sample holding unit 211, that is, the downward direction. The inclined surface 222 is inclined toward an inlet side wall portion 221 described later. That is, the inlet side wall portion 221 is provided on an extension line in the direction in which the inclined surface 222 extends.

The specimen holding unit 211 is a part that holds the specimen 81 that has flowed into the specimen quantitative channel 210. A wall portion extending in the upper right direction from the specimen holding portion 211 is an inlet side wall portion 221. A flow path inlet 220 communicating with the sample supply unit 212 is provided at the upper right end of the inlet side wall 221. The channel inlet 220 is an opening through which the sample 81 held in the sample holding unit 211 flows into the sample supply unit 212. The sample supply unit 212 is a channel extending downward from the channel inlet 220. The channel inlet 220 is provided closer to the sample introduction port 302 than the sample holder 211 in the fourth injection direction. The flow path inlet 220 of this embodiment is provided above the inclined surface 222.

The lower end of the sample supply unit 212 is connected to a sample guide unit 213 having a narrow channel. Below the sample guide unit 213, a sample determination unit 214 is provided. The specimen quantification unit 214 is a part that quantifies the specimen 81, and is a recess that is recessed in the lower left direction. A passage 215 extends to the lower left and a passage 217 extends to the upper right from a portion where the sample guide unit 213 and the sample determination unit 214 communicate with each other. The passage 215 extends to the sample surplus part 216 provided below the sample determination unit 214. The specimen surplus part 216 is a part where the specimen 81 overflowing from the specimen quantification part 214 is stored, and is a concave part provided in the right direction from the lower end part of the passage 215. The upper right end of the passage 217 is connected to a common channel 280 described later.

The reagent fixed amount flow path 230 includes a reagent holding unit 231, a reagent supply unit 232, a reagent guide unit 233, a reagent fixed amount unit 234, a passage 235, a reagent surplus portion 236, and a passage 237. A lower end portion of the reagent introduction port 304 is connected to the upper left portion of the reagent fixed amount flow channel 230. A reagent holding portion 231 that is a recess opening upward is provided at the lower right of the reagent introduction port 304. A wall portion extending in the upper right direction from the reagent holding portion 231 is an inlet side wall portion 241. The inlet side wall 241 is located below the reagent introduction port 304.

As will be described later, the first reagent 82 can flow from the reagent introduction port 304 into the reagent fixed amount flow channel 230 by moving to the lower left along the inlet side wall portion 241. That is, the inlet side wall part 241 is provided between the reagent introduction port 304 and the reagent holding part 231 and is inclined with respect to the downward direction which is the fifth injection direction. The fifth injection direction is a direction in which the capillary 404 is inserted into the reagent introduction port 304, in other words, a direction from the reagent introduction port 304 toward the reagent holding unit 231, that is, a downward direction.

The reagent holding part 231 is a part where the first reagent 82 that has flowed into the reagent fixed amount flow channel 230 is held. A flow path inlet 240 communicating with the reagent supply unit 232 is provided at the upper right end of the inlet side wall 241. The channel inlet 240 is an opening through which the first reagent 82 held in the reagent holding unit 231 flows into the reagent supply unit 232. The reagent supply unit 232 is a channel that extends downward from the channel inlet 240. The flow path inlet 240 is provided closer to the reagent introduction port 304 than the reagent holding part 231 in the fifth injection direction. The flow path inlet 240 of the present embodiment is provided above the portion of the inlet side wall 241 where the lower end of the capillary 404 contacts.

The lower end of the reagent supply unit 232 is connected to a reagent guide unit 233 having a narrow channel. A reagent quantitative unit 234 is provided below the reagent guide unit 233. The reagent quantification unit 234 is a part that quantifies the first reagent 82, and is a recess that is recessed downward and to the left. From the part where the reagent guide unit 233 and the reagent quantitative unit 234 communicate with each other, the passage 235 extends to the lower left and the passage 237 extends to the upper right. The passage 235 extends to the reagent surplus portion 236 provided below the reagent fixed amount portion 234. The reagent surplus portion 236 is a portion in which the first reagent 82 overflowing from the reagent quantitative portion 234 is stored, and is a concave portion provided in the right direction from the lower end portion of the passage 235. The upper right end of the passage 237 is connected to a common channel 280 described later.

The reagent fixed amount flow channel 250 includes a reagent holding unit 251, a reagent supply unit 252, a reagent guide unit 253, a reagent fixed amount unit 254, a channel 255, a reagent surplus unit 256, a channel 257, a capillary unit 262, and a connection channel 263. The capillary portion 262 is provided at the upper left end portion of the reagent fixed amount flow channel 250 and extends vertically below the reagent introduction port 306. A taper 268 is provided at the upper end of the capillary portion 262. Within the taper 268, the width of the channel in the left-right direction decreases toward the bottom. The capillary portion 262 and the reagent introduction port 306 communicate with each other in the vertical direction via the taper 268. A taper 269 is provided at the lower end of the capillary portion 262. Within the taper 269, the channel width in the left-right direction decreases downward. The connection channel 263 extends downward from the lower end of the taper 269. Below the connection channel 263, a reagent holding part 251 which is a concave part opening upward is provided. A protruding wall 264 that is a wall portion protruding from the lower end portion of the right wall of the connection channel 263 to the lower side of the channel inlet 260 is provided between the connection channel 263 and a channel inlet 260 described later. .

As will be described later, the second reagent 83 can flow into the reagent fixed amount flow channel 250 from the reagent introduction port 306 by moving downward along the taper 268. That is, the taper 268 is provided between the reagent introduction port 306 and the reagent holding part 251 and is inclined with respect to the downward direction which is the sixth injection direction. The sixth injection direction is a direction in which the capillary 406 is inserted into the reagent introduction port 306, in other words, a direction from the reagent introduction port 306 toward the reagent holding unit 251, that is, a downward direction.

The capillary part 262 is a part that is provided between the reagent introduction port 306 and the reagent holding part 251 and holds the second reagent 83 flowing out from the capillary 406 housed in the reagent introduction port 306 by capillary force. The connection channel 263 is a channel provided between the capillary part 262 and the reagent holding part 251. The reagent introduction port 306, the capillary part 262, and the connection channel 263 communicate with each other in the sixth injection direction.

As shown in FIG. 6, the outer diameter L1 which is the length in the left-right direction of the capillary 406 is smaller than the opening width L2 in the left-right direction of the reagent introduction port 306. The left and right flow path width L3 of the capillary portion 262 is smaller than the left and right opening width L2 of the reagent introduction port 306. That is, the cross section of the capillary portion 262 in the direction orthogonal to the sixth injection direction is smaller than the cross section of the capillary 406 in the direction orthogonal to the sixth injection direction, and further in the direction orthogonal to the sixth injection direction of the reagent inlet 306. Smaller than cross section. The left and right channel width L4 of the connection channel 263 is larger than the outer diameter L1 which is the length in the left and right direction of the capillary 406, and larger than the left and right opening width L2 of the reagent introduction port 306. That is, the cross section of the reagent introduction port 306 in the direction orthogonal to the sixth injection direction is smaller than the cross section of the connection channel 263 in the direction orthogonal to the sixth injection direction.

As shown in FIG. 4, the reagent holding unit 251 is a part where the second reagent 83 that has flowed into the reagent fixed amount flow channel 250 is held. A wall portion extending in the upper right direction from the reagent holding portion 251 is an inlet side wall portion 261. A flow path inlet 260 communicating with the reagent supply unit 252 is provided at the upper right end of the inlet side wall 261. The flow path inlet 260 is an opening through which the second reagent 83 held in the reagent holding unit 251 flows into the reagent supply unit 252. The reagent supply unit 252 is a channel that extends downward from the channel inlet 260. The channel inlet 260 is provided closer to the reagent inlet 306 than the reagent holding part 251 in the sixth injection direction. The channel inlet 260 of the present embodiment is provided above the lower end portion of the protruding wall 264.

The lower end of the reagent supply unit 252 is connected to a reagent guide unit 253 having a narrow channel. A reagent quantitative unit 254 is provided below the reagent guide unit 253. The reagent quantification unit 254 is a part that quantifies the second reagent 83, and is a recess that is recessed in the lower left direction. A passage 255 extends to the lower left and a passage 257 extends to the upper right from a portion where the reagent guide unit 253 and the reagent quantitative unit 254 communicate with each other. The passage 255 extends to the reagent surplus portion 256 provided below the reagent fixed amount portion 254. The reagent surplus portion 256 is a portion in which the second reagent 83 overflowing from the reagent fixed amount portion 254 is stored, and is a concave portion provided in the right direction from the lower end portion of the passage 255. The upper right end of the passage 257 is connected to a common channel 280 described later.

The common flow path 280 is a flow path that connects the

passages

217, 237, and 257 and the measurement unit 290. The common flow path 280 includes

guide walls

281, 282, and 283. The guide wall 281 is a wall portion provided on the right side of the passage 217 and extending in the lower right direction. The guide wall 282 is a wall portion provided on the right side of the passage 237 and extending in the lower right direction. The guide wall 283 is a wall portion provided on the right side of the passage 237 and extending downward to the measurement unit 290. The measurement unit 290 is a concave portion provided in the lower right portion of the common flow path 280 and recessed downward. The measurement unit 290 is a part where the liquid mixture 84 shown in FIG. 11 which is a liquid containing the specimen 81, the first reagent 82, and the second reagent 83 is stored and measured. When optical measurement to be described later is performed, measurement light is transmitted through the measurement unit 290.

<4-4. Other structures of inspection chip 2>
As shown in FIG. 1, the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates around the support shaft 46. When the inspection chip 2 is in the steady state shown in FIGS. 3 and 4, the upper side 21 and the lower side 24 are orthogonal to the direction of gravity G, the right side 22 and the left side 23 are parallel to the direction of gravity G, and The left side portion 23 is disposed closer to the main shaft 57 than the right side portion 22. In a state where the inspection chip 2 in the steady state is arranged at the measurement position, the inspection apparatus 1 performs inspection by optical measurement by allowing the measurement light connecting the light source 71 and the optical sensor 72 to pass through the

measurement units

190 and 290. . At this time, the

measurement units

190 and 290 are in a state of being aligned in the traveling direction of the measurement light. That is, the front-rear direction in which the

measurement units

190 and 290 are arranged is parallel to the traveling direction of the measurement light in the optical measurement.

<5. Example of inspection method>
An inspection method using the inspection apparatus 1 and the inspection chip 2 will be described with reference to FIGS. 7 to 11, portions of the liquid introduction unit 300 and the liquid channel 200 that do not correspond to the liquid channel 100 in the front-rear direction are indicated by dotted lines.

As shown in FIG. 6, the user inserts the capillary 401 holding the sample 61 into the opening 301A. At this time, the lower end portion 401A, which is the end portion in the first injection direction of the capillary 401 accommodated in the sample introduction port 301, is positioned by contacting the inclined surface 312. The inclined surface 312 is a surface that is provided between the sample introduction port 301 and the sample holder 111 and is inclined with respect to the first injection direction. The capillary 401 accommodated in the sample introduction port 301 is prevented from coming into contact with the inclined surface 312 and entering the sample holding unit 111. Therefore, the sample 61 flowing out from the positioned capillary 401 is likely to flow into the sample holding unit 111. Therefore, since the sample 61 is more reliably held in the sample holding unit 111, the sample 61 is less likely to move to the flow channel inlet 120 when the capillary 401 is inserted into the sample introduction port 301. Therefore, the possibility that the sample 61 flows out to the sample holding unit 111 and further flows into the sample supply unit 112 via the flow path inlet 120 at an unintended timing is suppressed. The intended timing, that is, the appropriate timing is the timing at which the measurement operation shown in FIG.

The user inserts the capillary 402 in which the specimen 81 is held in the opening 302A. At this time, the lower end portion 402 A that is the end portion of the capillary 402 accommodated in the sample introduction port 302 in the second injection direction is positioned by contacting the inclined surface 222. The inclined surface 222 is a surface that is provided between the sample introduction port 302 and the sample holding unit 211 and is inclined with respect to the second injection direction. The capillary 402 accommodated in the sample introduction port 302 is prevented from coming into contact with the inclined surface 222 and entering the sample holding unit 211. Accordingly, the sample 81 flowing out from the positioned capillary 402 is likely to flow into the sample holding unit 211. Therefore, since the sample 81 is more reliably held in the sample holding unit 211, the sample 81 is less likely to move to the flow channel inlet 220 when the capillary 402 is inserted into the sample introduction port 302. Therefore, the possibility that the sample 81 flows out to the sample holding unit 211 and further flows into the sample supply unit 212 via the flow path inlet 220 at an unintended timing is suppressed.

The user inserts the capillary 403 in which the first reagent 62 is held in the opening 303A. At this time, the left lower end portion 403A, which is the end portion in the third injection direction of the capillary 403 accommodated in the reagent introduction port 303, is positioned by contacting the inclined surface 314. The inclined surface 314 is provided between the reagent introduction port 303 and the reagent holding unit 131 and is an inclined surface with respect to the third injection direction. The capillary 403 accommodated in the reagent introduction port 303 is prevented from coming into contact with the inclined surface 314 and entering the reagent holding unit 131. Accordingly, the first reagent 62 flowing out from the positioned capillary 403 is likely to flow into the reagent holding unit 131. Therefore, since the first reagent 62 is more reliably held in the reagent holding part 131, the first reagent 62 is less likely to move to the channel inlet 140 when the capillary 403 is inserted into the reagent introduction port 303. Therefore, the possibility that the first reagent 62 flows out to the reagent holding unit 131 and further flows into the reagent supply unit 132 through the flow path inlet 140 at an unintended timing is suppressed.

The user inserts the capillary 404 in which the first reagent 82 is held in the opening 304A. At this time, the lower end 404A, which is the end in the fourth injection direction of the capillary 404 accommodated in the reagent introduction port 304, is positioned by contacting the inlet side wall 241. The inlet side wall portion 241 is a surface that is provided between the reagent introduction port 304 and the reagent holding portion 231 and is inclined with respect to the fourth injection direction. The capillary 404 accommodated in the reagent introduction port 304 is prevented from coming into contact with the inlet side wall 241 and entering the reagent holding unit 231. Therefore, the first reagent 82 flowing out from the positioned capillary 404 is likely to flow into the reagent holding unit 231. Therefore, since the first reagent 82 is more reliably held in the reagent holding unit 231, the first reagent 82 is less likely to move to the channel inlet 240 when the capillary 404 is inserted into the reagent introduction port 304. Therefore, the possibility that the first reagent 82 flows out to the reagent holding unit 231 and further flows into the reagent supply unit 232 via the flow path inlet 240 at an unintended timing is suppressed.

The user inserts the capillary 405 in which the second reagent 63 is held in the opening 305A. At this time, the lower end portion 405A, which is the end portion in the fifth injection direction of the capillary 405 accommodated in the reagent introduction port 305, is positioned by contacting the inclined surface 316. The inclined surface 316 is a surface that is provided between the reagent introduction port 305 and the reagent holding unit 151 and is inclined with respect to the fifth injection direction. The capillary 405 accommodated in the reagent inlet 305 is prevented from coming into contact with the inclined surface 316 and entering the reagent holding unit 151. Therefore, the second reagent 63 flowing out from the positioned capillary 405 is likely to flow into the reagent holding unit 151. Therefore, since the second reagent 63 is more reliably held in the reagent holding part 151, the second reagent 63 is unlikely to move to the flow path inlet 160 when the capillary 405 is inserted into the reagent introduction port 305. Therefore, the possibility that the second reagent 63 flows out to the reagent holding unit 151 and further flows into the reagent supply unit 152 via the flow path inlet 160 at an unintended timing is suppressed.

The user inserts the capillary 406 in which the second reagent 83 is held in the opening 306A. The cross section of the capillary portion 262 in the direction orthogonal to the sixth injection direction is smaller than the cross section of the capillary 406 in the direction orthogonal to the sixth injection direction. Therefore, the lower end portion 406A, which is the end portion in the sixth injection direction of the capillary 406 accommodated in the reagent introduction port 306, is positioned by contacting the taper 268. The capillary 406 accommodated in the reagent introduction port 306 is prevented from coming into contact with the taper 268 and entering the reagent holding unit 251. Therefore, the second reagent 83 flowing out from the positioned capillary 406 tends to flow into the reagent holding unit 251. Therefore, since the second reagent 83 is more reliably held in the reagent holding unit 251, the second reagent 83 is unlikely to move to the flow path inlet 260 when the capillary 406 is inserted into the reagent introduction port 306. Therefore, the possibility that the second reagent 83 flows out to the reagent holding unit 251 and further flows into the reagent supply unit 252 via the flow path inlet 260 at an unintended timing is suppressed.

The taper 268 is a surface that is provided between the reagent introduction port 306 and the reagent holding unit 251 and is inclined with respect to the sixth injection direction. The second reagent 83 that flows out from the capillary 406 flows smoothly into the capillary portion 262 along the taper 268. Therefore, since the second reagent 83 is more reliably held in the capillary portion 262, the second reagent 83 is unlikely to move to the flow path inlet 260 when the capillary 406 is inserted into the reagent inlet 306. Therefore, the possibility that the second reagent 83 flows out to the reagent holding unit 251 and further flows into the reagent supply unit 252 via the flow path inlet 260 at an unintended timing is suppressed.

The second reagent 83 supplied into the reagent introduction port 306 is sucked by the capillary force generated in the capillary part 262 and held in the capillary part 262. Therefore, when the capillary 406 is accommodated in the reagent introduction port 306, the second reagent 83 flowing out from the capillary 406 is prevented from flowing into the flow path inlet 260. Therefore, the possibility that the second reagent 83 flows out to the reagent holding unit 251 and further flows into the reagent supply unit 252 via the flow path inlet 260 at an unintended timing is suppressed.

The flow path width L3 that is a cross section in the direction orthogonal to the sixth injection direction of the capillary portion 262 is the opening width L2 that is a cross section in the direction orthogonal to the sixth injection direction of the reagent introduction port 306 and the first flow path 263. It is smaller than the channel width L4 which is a cross section in a direction orthogonal to the six injection directions. That is, among the opening width L2 of the reagent inlet 306, the flow path width L3 of the capillary section 262, and the flow path width L4 of the connection flow path 263, the flow path width L3 of the capillary section 262 is the smallest. Therefore, the capillary force generated in the capillary portion 262 is larger than the capillary force generated in the reagent introduction port 306 and the capillary force generated in the connection channel 263. Accordingly, the second reagent 83 flowing out from the capillary 406 is held in the capillary portion 262 in preference to the reagent introduction port 306 and the connection channel 263. Therefore, the possibility that the second reagent 83 flows out to the reagent holding unit 251 and further flows into the reagent supply unit 252 via the flow path inlet 260 at an unintended timing is suppressed.

The opening width L2 which is a cross section in the direction orthogonal to the sixth injection direction of the reagent introduction port 306 is smaller than the flow path width L4 which is a cross section in the direction orthogonal to the sixth injection direction of the connection flow path 263. That is, the opening width L2 of the reagent introduction port 306 is smaller than the channel width L4 of the connection channel 263. Therefore, the capillary force generated in the reagent introduction port 306 is larger than the capillary force generated in the connection channel 263. Therefore, the surplus second reagent 83 that cannot be held by the capillary portion 262 is held not in the connection channel 263 but in the reagent introduction port 306. Therefore, before the measurement operation described later is executed, the surplus second reagent 83 is prevented from flowing from the connection channel 263 to the channel inlet 260. As described above, the second reagent 83 flowing out from the capillary 406 is held on the reagent inlet 306 side with respect to the connection channel 263. As a result, the possibility that the second reagent 83 flows into the reagent supply unit 252 via the flow path inlet 260 at an unintended timing is suppressed.

Next, as shown in FIG. 1, when the inspection chip 2 is attached to the spindle 46 and a processing start command is input to the control device 90, the following measurement operation is executed. The inspection apparatus 1 can inspect two inspection chips 2 at the same time. For convenience of explanation, a procedure for inspecting one inspection chip 2 will be described below. In the following description, the steady state of the inspection chip 2 shown in FIGS. 3 and 4 is defined as a rotation angle of 0 degree, and the state rotated 90 degrees counterclockwise from the steady state is defined as a rotation angle of 90 degrees.

First, based on an instruction from the CPU 91, the rotation controller 98 drives and controls the stepping motor 51, whereby the inspection chip 2 in a steady state rotates 90 degrees counterclockwise when viewed from the front. Based on the instruction from the CPU 91, the revolution controller 97 controls the spindle motor 35 to start driving the turntable 33. In the present embodiment, the front direction of the test chip 2 is the direction of revolution. Based on the instruction from the CPU 91, the revolution controller 97 controls the spindle motor 35 to increase the rotation speed of the turntable 33. When the rotational speed reaches, for example, 3000 rpm, the spindle motor 35 maintains this rotational speed. Thereby, as shown in FIG. 7, the test | inspection chip 2 whose autorotation angle is 90 degree | times is revolved. Centrifugal force X acts on the inspection chip 2 from the upper side 21 toward the lower side 24. Due to the action of the centrifugal force X, the position of the liquid changes in the inspection chip 2 as follows.

With reference to FIG. 7, the change in the liquid position in the liquid channel 100 will be described. Due to the action of the centrifugal force X, the sample 61 flows downward from the capillary 401 accommodated in the sample introduction port 301 shown in FIG. An inclined surface 312 is provided that is inclined in a direction from the front surface 25 toward the rear surface 26 and communicates the front surface 25 side and the rear surface 26 side. The specimen 61 flows from the specimen introduction port 301 into the specimen quantitative channel 110 by moving through the guide hole 311 along the inclined surface 312. The sample 61 moves downward from the end 312 A of the inclined surface 312 and is held by the sample holding unit 111. Therefore, since the sample 61 is more reliably held in the sample holding unit 111, the sample 61 is less likely to move to the flow channel inlet 120 when the capillary 401 is inserted into the sample introduction port 301. Therefore, the possibility that the sample 61 flows out to the sample holding unit 111 and further flows into the sample supply unit 112 via the flow path inlet 120 at an unintended timing is suppressed.

The flow channel inlet 120 is provided closer to the sample introduction port 301 than the sample holding unit 111 in the first injection direction from the sample introduction port 301 toward the sample holding unit 111. Thus, the sample 61 flowing out from the capillary 401 accommodated in the sample introduction port 301 is held in the sample holding unit 111 before flowing into the flow path inlet 120. Therefore, the specimen 61 is unlikely to move to the flow path inlet 120 when the capillary 401 is inserted into the specimen introduction port 301. As a result, the sample 61 flowing out from the capillary 401 can be moved from the sample holding unit 111 to the flow channel inlet 120 at an appropriate timing and can flow into the sample supply unit 112. As a result, the possibility that the sample 61 flows into the sample supply unit 112 via the channel inlet 120 at an unintended timing is suppressed.

As shown in FIG. 2, the flow path width L5, which is the width in the direction in which the front surface 25 and the rear surface 26 of the flow path inlet 120 face each other, is a flow that is the width in the direction in which the front surface 25 and the rear surface 26 of the specimen holder 111 face each other. It is smaller than the road width L6. Accordingly, when the sample 61 moves from the sample holding unit 111 to the flow channel inlet 120 side at an unintended timing, it is difficult for the sample 61 to pass through the flow channel inlet 120. As a result, the sample can be moved from the sample holding unit 111 to the flow path inlet 120 at an appropriate timing and can flow into the sample supply unit 112.

The first reagent 62 flows downward from the capillary 403 accommodated in the reagent introduction port 303 shown in FIG. An inclined surface 314 that is inclined in a direction from the front surface 25 toward the rear surface 26 and communicates the front surface 25 side and the rear surface 26 side is provided. The first reagent 62 flows through the guide hole 313 along the inclined surface 314 and flows into the reagent fixed amount flow path 130 from the reagent introduction port 303. The first reagent 62 moves downward from the end 314 A of the inclined surface 314 and is held by the reagent holding unit 131. Therefore, since the first reagent 62 is more reliably held in the reagent holding part 131, the first reagent 62 is less likely to move to the channel inlet 140 when the capillary 403 is inserted into the reagent introduction port 303. Therefore, the possibility that the first reagent 62 flows out to the reagent holding unit 131 and further flows into the reagent supply unit 132 through the flow path inlet 140 at an unintended timing is suppressed.

The flow path inlet 140 is provided closer to the reagent introduction port 303 than the reagent holding unit 131 in the third injection direction from the reagent introduction port 303 toward the reagent holding unit 131. Thus, the first reagent 62 flowing out from the capillary 403 accommodated in the reagent introduction port 303 is held in the reagent holding unit 131 before flowing into the flow path inlet 140. Therefore, the first reagent 62 is difficult to move to the flow path inlet 140 when the capillary 403 is inserted into the reagent introduction port 303. Thus, the user can move the first reagent 62 flowing out from the capillary 403 from the reagent holding unit 131 to the flow path inlet 140 at an appropriate timing and flow into the reagent supply unit 132. As a result, the possibility that the first reagent 62 flows into the reagent supply unit 132 via the flow path inlet 140 at an unintended timing is suppressed.

The guide hole 313 is located at the lower left end which is the end of the reagent introduction port 303 in the third injection direction. In FIG. 7, the imaginary line extending from the guide hole 313 toward the inlet side wall 141 indicates the imaginary direction K. The virtual direction K is a virtual direction extending in parallel with the front surface 25 and the rear surface 26 from the end of the reagent introduction port 303 in the third injection direction and extending perpendicularly to the third injection direction. Of the angles formed by the virtual direction K and the inlet side wall 141, the angle θ formed between the guide hole 313 and the flow path inlet 140 is 90 degrees or less.

The first reagent 62 that has flowed out of the capillary 403 accommodated in the reagent introduction port 303 may move in the virtual direction K due to, for example, vibration of the test chip 2. When the angle θ is 90 degrees or less, the first reagent 62 moved in the virtual direction K is guided to the reagent holding unit 131 provided on the side opposite to the flow path inlet 140 by the inlet side wall 141. Therefore, the first reagent 62 flowing out from the capillary 403 is suppressed from moving to the flow path inlet 140 beyond the inlet side wall portion 141. As a result, the possibility that the first reagent 62 flows into the reagent supply unit 132 via the flow path inlet 140 at an unintended timing is suppressed.

By the action of the centrifugal force X, the second reagent 63 flows downward from the capillary 405 accommodated in the reagent inlet 305 shown in FIG. An inclined surface 316 that is inclined in a direction from the front surface 25 toward the rear surface 26 and that communicates the front surface 25 side and the rear surface 26 side is provided. The second reagent 63 flows through the guide hole 315 along the inclined surface 316 and flows into the reagent fixed amount flow path 150 from the reagent introduction port 305. The second reagent 63 moves downward from the end 316A of the inclined surface 316. The second reagent 63 moves downward from the end portion 316A of the inclined surface 316 and is held by the reagent holding portion 151. Therefore, since the second reagent 63 is more reliably held in the reagent holding part 151, the second reagent 63 is unlikely to move to the flow path inlet 160 when the capillary 405 is inserted into the reagent introduction port 305. Therefore, the possibility that the second reagent 63 flows out to the reagent holding unit 151 and further flows into the reagent supply unit 152 via the flow path inlet 160 at an unintended timing is suppressed.

A step that protrudes in the direction opposite to the front surface 25 on the rear surface 26 from the end portion 316A on the front surface 25 side of the inclined surface 316 or an inclination in the opposite direction to the front surface 25 on the rear surface 26. A constricted portion 165 is provided. Since the narrow portion 165 has a small flow path width in the front-rear direction in which the front surface 25 and the rear surface 26 face each other, a capillary force is generated. The second reagent 63 moving downward from the end portion 316A is sucked by the capillary force generated in the narrowed portion 165 and guided to the reagent holding portion 151. That is, the second reagent 63 guided to the front surface 25 side by the inclined surface 316 flows into the reagent holding portion 151 by the capillary force generated in the narrowed portion 165. As a result, the possibility that the second reagent 63 moved from the rear surface 26 side to the front surface 25 side flows into the reagent supply unit 152 through the flow path inlet 160 at an unintended timing is suppressed.

The flow channel inlet 160 is provided closer to the reagent introduction port 305 than the reagent holding unit 151 in the fifth injection direction from the reagent introduction port 305 toward the reagent holding unit 151. Thereby, the second reagent 63 flowing out from the capillary 405 accommodated in the reagent introduction port 305 is held in the reagent holding unit 151 before flowing into the flow path inlet 160. Therefore, the second reagent 63 is difficult to move to the flow path inlet 160 when the capillary 405 is inserted into the reagent inlet 305. As a result, the user can move the second reagent 63 flowing out from the capillary 405 from the reagent holding unit 151 to the flow channel inlet 160 at an appropriate timing and flow into the reagent supply unit 152. As a result, the possibility that the second reagent 63 flows into the reagent supply unit 152 via the flow path inlet 160 at an unintended timing is suppressed.

The lower part of the constriction part 165 is provided in the reagent holding part 151. A capillary force resulting from the constriction 165 is also generated in the reagent holding unit 151. The reagent holding unit 151 holds the second reagent 63 that has flowed out of the capillary 405 due to the capillary force resulting from the constriction 165. Accordingly, the second reagent 63 held by the reagent holding unit 151 is prevented from flowing out of the reagent holding unit 151 due to, for example, vibration of the test chip 2. As a result, the possibility that the second reagent 63 flows into the reagent supply unit 152 via the flow path inlet 160 at an unintended timing is suppressed.

With reference to FIG. 4 and FIG. 7, the change of the liquid position in the liquid flow path 200 will be described. Due to the action of the centrifugal force X, the sample 81 flows downward from the capillary 402 accommodated in the sample introduction port 302 shown in FIG. The sample 81 flows into the sample determination channel 210 and moves to the lower right along the inclined surface 222. Since the inclined surface 222 is inclined toward the inlet side wall 221, the specimen 81 is guided toward the inlet side wall 221 by the inclined surface 222. The sample 81 moves to the lower left along the inlet side wall portion 221 and is held by the sample holding portion 211. That is, the inclined surface 222 guides the specimen 81 that has flowed out of the capillary 402 toward the inlet side wall portion 221 that guides the specimen 81 toward the specimen holding portion 211. Therefore, the possibility that the sample 81 flows into the sample supply unit 212 via the flow path inlet 220 at an unintended timing is suppressed.

The flow path inlet 220 is provided closer to the sample introduction port 302 than the sample holding unit 211 in the second injection direction from the sample introduction port 302 toward the sample holding unit 211. Thus, the sample 81 flowing out from the capillary 402 accommodated in the sample introduction port 302 is held in the sample holding unit 211 before flowing into the flow path inlet 220. Therefore, the specimen 81 is unlikely to move to the flow path inlet 220 when the capillary 402 is inserted into the specimen introduction port 302. As a result, the user can move the sample 81 flowing out from the capillary 402 from the sample holding unit 211 to the flow channel inlet 220 at an appropriate timing and flow into the sample supply unit 212. As a result, the possibility that the sample 81 flows into the sample supply unit 212 via the flow path inlet 220 at an unintended timing is suppressed.

The first reagent 82 flows downward from the capillary 404 accommodated in the reagent introduction port 304 shown in FIG. 6 by the action of the centrifugal force X. As a result, the first reagent 82 flows into the reagent fixed amount flow channel 230, moves to the lower right along the inlet side wall portion 241, and is then held in the reagent holding portion 231. The channel inlet 240 is provided closer to the reagent introduction port 304 than the reagent holding unit 231 in the fourth injection direction from the reagent introduction port 304 toward the reagent holding unit 231. Thus, the first reagent 82 flowing out from the capillary 404 accommodated in the reagent introduction port 304 is held in the reagent holding unit 231 before flowing into the flow path inlet 240. Therefore, the first reagent 82 is unlikely to move to the flow path inlet 240 when the capillary 404 is inserted into the reagent inlet 304. As a result, the user can move the first reagent 82 flowing out from the capillary 404 from the reagent holding unit 231 to the flow path inlet 240 at an appropriate timing and flow into the reagent supply unit 232. As a result, the possibility that the first reagent 82 flows into the reagent supply unit 232 via the flow path inlet 240 at an unintended timing is suppressed.

The second reagent 83 held in the capillary part 262 flows downward by the action of the centrifugal force X. The second reagent 83 moves downward via the connection channel 263 and is held by the reagent holding unit 251. A protruding wall 264 is provided between the connection channel 263 and the channel inlet 260 so as to protrude below the channel inlet 260. Even when the second reagent 83 existing in the connection channel 263 moves to the channel inlet 260 side due to vibration of the test chip 2, for example, the protruding wall 264 prevents the second reagent 83 from flowing into the channel inlet 260. Is done.

The flow path inlet 260 is provided closer to the reagent inlet 306 than the reagent holder 251 in the sixth injection direction from the reagent inlet 306 toward the reagent holder 251. Thus, the second reagent 83 flowing out from the capillary 406 accommodated in the reagent introduction port 306 is held in the reagent holding unit 251 before flowing into the flow path inlet 260. Therefore, the second reagent 83 is unlikely to move to the flow path inlet 260 when the capillary 406 is inserted into the reagent inlet 306. As a result, the user can move the second reagent 83 flowing out of the capillary 406 from the reagent holding unit 251 to the flow path inlet 260 at an appropriate timing and flow into the reagent supply unit 252. As a result, the possibility that the second reagent 83 flows into the reagent supply unit 252 via the flow path inlet 260 at an unintended timing is suppressed.

Next, the rotation controller 98 drives and controls the stepping motor 51 based on an instruction from the CPU 91, so that the inspection chip 2 that is revolving is rotated 90 degrees clockwise as viewed from the front. Thereby, as shown in FIG. 8, the test | inspection chip 2 whose autorotation angle is 0 degree | times is revolved. A centrifugal force acts on the test chip 2 from the left side 23 toward the right side 22. Due to the action of the centrifugal force X, the position of the liquid changes in the inspection chip 2 as follows.

With reference to FIG. 4 and FIG. 8, the change of the liquid position in the liquid flow path 100 and the liquid flow path 200 is demonstrated. In the sample quantification channel 110, the sample 61 moves to the upper right along the inlet side wall 121 by the action of the centrifugal force X, and flows into the sample supply unit 112 through the channel inlet 120. In the reagent fixed amount flow path 130, the first reagent 62 moves to the upper right along the inlet side wall portion 141 by the action of the centrifugal force X, and flows into the reagent supply portion 132 via the flow path inlet 140. In the reagent fixed amount flow path 150, the second reagent 63 moves to the upper right along the inlet side wall portion 161 by the action of the centrifugal force X, and flows into the reagent supply portion 152 through the flow path inlet 160. In the sample quantification channel 210, the sample 81 moves to the upper right along the inlet side wall 221 by the action of the centrifugal force X, and flows into the sample supply unit 212 through the channel inlet 220. In the reagent fixed amount flow channel 230, the first reagent 82 moves to the upper right along the inlet side wall 241 by the action of the centrifugal force X, and flows into the reagent supply unit 232 through the flow channel inlet 240. In the reagent fixed amount flow channel 250, the second reagent 83 moves to the upper right along the inlet side wall portion 261 by the action of the centrifugal force X, and flows into the reagent supply portion 252 through the flow channel inlet 260.

Next, the rotation controller 98 drives and controls the stepping motor 51 based on an instruction from the CPU 91, so that the inspection chip 2 that is revolving is rotated 90 degrees counterclockwise when viewed from the front. Thereby, as shown in FIG. 9, the test | inspection chip 2 whose autorotation angle is 90 degree | times is revolved. A centrifugal force acts on the inspection chip 2 from the upper side 21 toward the lower side 24. Due to the action of the centrifugal force X, the position of the liquid changes in the inspection chip 2 as follows.

4 and 9, changes in the liquid position in the liquid channel 100 and the liquid channel 200 will be described. In the sample quantification channel 110, the sample 61 is supplied to the sample quantification unit 114 via the sample guide unit 113 by the action of the centrifugal force X. The surplus specimen 61 overflowing from the specimen quantification part 114 flows into the specimen surplus part 116 via the passage 115. As a result, the sample 61 corresponding to the volume of the sample quantitative unit 114 remains in the sample quantitative unit 114. In the reagent fixed amount flow path 130, the first reagent 62 is supplied to the reagent fixed amount section 134 via the reagent guide section 133 by the action of the centrifugal force X. The surplus first reagent 62 overflowing from the reagent quantitative unit 134 flows into the reagent surplus unit 136 via the passage 135. The first reagent 62 corresponding to the volume of the reagent quantitative unit 134 remains in the reagent quantitative unit 134. In the reagent quantitative flow path 150, the second reagent 63 is supplied to the reagent quantitative unit 154 through the reagent guide unit 153 by the action of the centrifugal force X. The surplus second reagent 63 overflowing from the reagent quantification unit 154 flows into the reagent surplus unit 156 via the passage 155. The second reagent 63 corresponding to the capacity of the reagent quantitative unit 154 remains in the reagent quantitative unit 154. In the sample quantification channel 210, the sample 81 is supplied to the sample quantification unit 214 via the sample guide unit 213 by the action of the centrifugal force X. The surplus sample 81 overflowing from the sample quantification unit 214 flows into the sample surplus unit 216 via the passage 215. As a result, the sample 81 corresponding to the volume of the sample quantitative unit 214 remains in the sample quantitative unit 214. In the reagent quantitative flow channel 230, the first reagent 82 is supplied to the reagent quantitative unit 234 via the reagent guide unit 233 by the action of the centrifugal force X. The surplus first reagent 82 overflowing from the reagent quantitative unit 234 flows into the reagent surplus unit 236 via the passage 235. The first reagent 82 corresponding to the volume of the reagent quantitative unit 234 remains in the reagent quantitative unit 234. In the reagent quantitative flow channel 250, the second reagent 83 is supplied to the reagent quantitative unit 254 via the reagent guide unit 253 by the action of the centrifugal force X. The surplus second reagent 83 overflowing from the reagent quantification unit 254 flows into the reagent surplus unit 256 via the passage 255. The second reagent 83 corresponding to the capacity of the reagent quantitative unit 254 remains in the reagent quantitative unit 254.

Next, the rotation controller 98 drives and controls the stepping motor 51 based on an instruction from the CPU 91, so that the inspection chip 2 that is revolving is rotated 90 degrees clockwise as viewed from the front. Thereby, as shown in FIG. 10, the test | inspection chip 2 whose autorotation angle is 0 degree | times is revolved. A centrifugal force acts on the test chip 2 from the left side 23 toward the right side 22. Due to the action of the centrifugal force X, the position of the liquid changes in the inspection chip 2 as follows.

With reference to FIG. 4 and FIG. 10, the change of the liquid position in the liquid flow path 100 and the liquid flow path 200 is demonstrated. In the sample quantification channel 110, the sample 61 remaining in the sample quantification unit 114 flows into the common channel 180 via the passage 117 by the action of the centrifugal force X. At this time, the specimen 61 moves to the lower right along the guide wall 181, and further moves to the right to the guide wall 183. The surplus specimen 61 that has flowed into the specimen surplus section 116 is held in the specimen surplus section 116. In the reagent fixed amount flow channel 130, the first reagent 62 remaining in the reagent fixed amount portion 134 flows into the common flow channel 180 through the passage 137 by the action of the centrifugal force X. At this time, the first reagent 62 moves to the lower right along the guide wall 182, and further moves to the right to the guide wall 183. The surplus first reagent 62 that has flowed into the reagent surplus portion 136 is held in the reagent surplus portion 136. In the reagent fixed amount flow channel 150, the second reagent 63 remaining in the reagent fixed amount portion 154 flows into the common flow channel 180 through the passage 157 by the action of the centrifugal force X, and moves rightward to the guide wall 183. The surplus second reagent 63 that has flowed into the reagent surplus portion 156 is held in the reagent surplus portion 156. The specimen 61, the first reagent 62, and the second reagent 63 that have moved to the guide wall 183 are mixed by the action of the centrifugal force X, and a mixed liquid 64 is generated. In the sample quantification channel 210, the sample 81 remaining in the sample quantification unit 214 flows into the common channel 280 via the passage 217 by the action of the centrifugal force X. At this time, the specimen 81 moves to the lower right along the guide wall 281, and further moves to the right to the guide wall 283. The surplus specimen 81 that has flowed into the specimen surplus part 216 is held in the specimen surplus part 216. In the reagent fixed amount flow channel 230, the first reagent 82 remaining in the reagent fixed amount portion 234 flows into the common flow channel 280 through the passage 237 by the action of the centrifugal force X. At this time, the first reagent 82 moves to the lower right along the guide wall 282, and further moves to the right to the guide wall 283. The surplus first reagent 82 that has flowed into the reagent surplus portion 236 is held in the reagent surplus portion 236. In the reagent fixed amount flow channel 250, the second reagent 83 remaining in the reagent fixed amount portion 254 flows into the common flow channel 280 through the passage 257 by the action of the centrifugal force X and moves to the right to the guide wall 283. The surplus second reagent 83 that has flowed into the reagent surplus portion 256 is held in the reagent surplus portion 256. The specimen 81, the first reagent 82, and the second reagent 83 that have moved to the guide wall 283 are mixed by the action of the centrifugal force X, and a mixed solution 84 is generated.

Then, based on the instruction from the CPU 91, the revolution controller 97 drives the spindle motor 35 to decelerate. The revolution controller 97 stops the spindle motor 35 after rotating the inspection chip 2 to the angle of the measurement position. Thereby, as shown in FIG. 11, the revolution of the test | inspection chip 2 whose autorotation angle is 0 degree | times is stopped. Gravity G acts on the inspection chip 2 from the upper side 21 toward the lower side 24. In the liquid channel 100, the mixed solution 64 is moved and stored in the measurement unit 190 by the action of gravity G. As shown in FIGS. 4 and 11, in the liquid flow path 200, the mixed solution 84 is moved and stored in the measurement unit 290 by the action of gravity G.

As shown in FIG. 1, when the inspection chip 2 is at the measurement position, the

measurement units

190 and 290 are arranged in the traveling direction of the measurement light emitted by the light source 71. When the measurement controller 99 emits the light source 71 based on an instruction from the CPU 91, the measurement light passes through the mixed solution 64 stored in the measurement unit 190 and the mixed solution 84 stored in the measurement unit 290. The CPU 91 measures the

mixed liquids

64 and 84 based on the change amount of the measurement light received by the optical sensor 72. In the present embodiment, different components are measured from the

mixed liquids

64 and 84 based on the wavelength of the light transmitted through the

mixed liquids

64 and 84.

Specifically, the mixed solution 64 is a mixed solution for measuring glucose and reacts with a light component having a wavelength of 340 nm. The mixed solution 84 is a mixed solution for measuring total cholesterol and reacts with a light component having a wavelength of 650 nm. The optical sensor 72 detects a light component having a wavelength of 340 nm through a bandpass filter (not shown). The CPU 91 measures glucose from the mixed solution 64 based on the light component having a wavelength of 340 nm detected by the optical sensor 72. The optical sensor 72 detects a light component having a wavelength of 650 nm through a bandpass filter (not shown). The CPU 91 measures total cholesterol from the mixed solution 84 based on the light component with a wavelength of 650 nm detected by the optical sensor 72. The CPU 91 displays the measurement result on the display 96. In addition, the measuring method of the

liquid mixtures

64 and 84 is not restricted to an optical measurement, Other methods may be used.

<6. Other>
In the above-described embodiment, the

sample introduction ports

301 and 302 and the

reagent introduction ports

303, 304, 305, and 306 respectively correspond to “introduction ports” of the present disclosure. The

sample holding units

111 and 211 and the

reagent holding units

131, 151, 231, and 251 correspond to the “holding unit” of the present disclosure. The

sample supply units

112 and 212 and the

reagent supply units

132, 152, 232, and 252 each correspond to a “fluid circuit” of the present disclosure. The

inclined surfaces

222, 312, 314, 316, the inlet side wall portion 241, and the taper 268 each correspond to an “inclined surface” of the present disclosure. The rear surface 26 corresponds to a “first surface” of the present disclosure. The front surface 25 corresponds to a “second surface” of the present disclosure.

(1) The liquid channels provided in the front surface 25 are not limited to the three channels of the sample quantification channel 110 and the

reagent quantification channels

130 and 150, and may be one channel or a plurality of channels. The liquid channels provided on the rear surface 26 are not limited to the three channels of the sample quantification channel 210 and the

reagent quantification channels

230 and 250, and may be one channel or a plurality of channels. A liquid channel may be provided only on one of the front surface 25 and the rear surface 26.

The inlets of the liquid inlet 300 are not limited to the six inlets of the

specimen inlets

301 and 302 and the

reagent inlets

303, 304, 305, and 306. One inlet may supply liquid to two or more liquid flow paths. The introduction port of the liquid introduction unit 300 may be provided on the front surface 25 or may be provided on both the front surface 25 and the rear surface 26.

(2) At least one of the liquid channels provided on the front surface 25 or the rear surface 26 may have any one of the sample

quantitative channels

110 and 210 and the reagent

quantitative channels

130, 150, 230, and 250. For example, it is assumed that the test chip 2 has the sample quantitative channel 110, the reagent quantitative channel 130, and the sample inlet 301 formed on the front surface 25 and the reagent inlet 303 formed on the rear surface 26. In the test chip 2, the sample determination channel 110 may be supplied with the sample 61 via the inclined surface 312, and the sample holding unit 111 may have a constriction 165. In the reagent fixed amount flow path 130, the capillary 403 may be positioned by an inclined surface 222 inclined toward the inlet side wall portion 141.

(3) The

specimens

61 and 81 are not limited to blood. The

specimens

61 and 81 may be, for example, a liquid containing components such as plasma, blood cells, bone marrow, urine, vaginal tissue, epithelial tissue, tumor, semen, saliva, or foodstuff. The

first reagent

62 and 82 and the

second reagent

63 and 83 may be appropriately changed according to the

specimens

61 and 81 and the inspection purpose. The inspection chip 2 may not include the

sheets

27 and 28. For example, the inspection chip 2 in which the

liquid flow paths

100 and 200 and the liquid introduction part 300 are directly formed inside the plate member 20 may be used.

2 Test chip 25 Front surface 26

Rear surface

61, 81

Sample

62, 82

First reagent

63, 83

Second reagent

111, 211

Sample holding unit

112, 212

Sample supply unit

120, 140, 160, 220, 240, 260

Channel inlet

121 , 141, 161, 221, 241, 261 Inlet

side wall part

131, 151, 231, 251

Reagent holding part

132, 152, 232, 252 Reagent supply part 165

Narrow part

190, 290 Measuring

part

222, 312, 314, 316 Inclined surface 262 Capillary portion 263

Connection channel

301, 302

Sample introduction port

303, 304, 305, 306

Reagent introduction port

401, 402, 403, 404, 405, 406 Capillary

Claims

An opening through which a liquid introducing means for holding a specimen or a reagent inside by a capillary force can be inserted, and an inlet that is a part in which the liquid introducing means inserted from the opening is accommodated;
A holding portion which is provided on the opposite side of the opening with respect to the inlet and is a part for holding the specimen or the reagent flowing out from the liquid introducing means accommodated in the inlet;
A fluid circuit that is a flow path through which the specimen or the reagent is guided toward a site where the liquid containing the specimen or the reagent is measured;
A flow path inlet that is an opening through which the specimen or the reagent held in the holding unit flows into the fluid circuit;
The inspection chip, wherein the flow path inlet is provided closer to the introduction port than the holding unit in an injection direction which is a direction from the introduction port toward the holding unit.
The inspection chip according to claim 1, further comprising an inclined surface that is provided between the introduction port and the holding portion and is an inclined surface with respect to the injection direction.
The inspection chip has a first surface and a second surface facing the first surface,
The introduction port is provided on the first surface side,
The holding portion, the fluid circuit, and the flow path inlet are provided on the second surface side,
The inspection chip according to claim 2, wherein the inclined surface is inclined in a direction from the first surface toward the second surface to communicate the first surface side with the second surface side. .
A capillary portion provided between the introduction port and the holding portion and configured to hold the sample or the reagent flowing out from the liquid introduction means accommodated in the introduction port by a capillary force; The test chip according to claim 1, wherein the test chip is a test chip.
The inspection chip according to claim 4, further comprising a taper provided at an end of the capillary portion on the introduction port side and inclined with respect to the injection direction.
The introduction port and the capillary portion communicate with the injection direction,
The test chip according to claim 4 or 5, wherein a cross section of the capillary section in a direction orthogonal to the injection direction is smaller than a cross section of the liquid introducing means in a direction orthogonal to the injection direction.
Provided between the capillary part and the holding part, provided with a connection flow path that is a flow path of the sample or the reagent,
The introduction port, the capillary part, and the connection channel communicate with the injection direction,
The cross section of the capillary section in the direction orthogonal to the injection direction is smaller than the cross section of the introduction port in the direction orthogonal to the injection direction,
The inspection chip according to any one of claims 4 to 6, wherein a cross section of the introduction port in a direction orthogonal to the injection direction is smaller than a cross section of the connection channel in a direction orthogonal to the injection direction. .
An inlet side wall part that is a wall part extending from the holding part toward the flow path inlet;
The inspection chip according to claim 2, wherein the inclined surface is inclined toward the inlet side wall portion.
The test chip according to any one of claims 1 to 8, wherein the holding unit holds the specimen or the reagent by capillary force.
A step that is provided closer to the holding portion than the end of the inclined surface on the second surface side and protrudes in the opposite direction to the first surface on the second surface or the opposite direction on the second surface. The inspection chip according to claim 3, further comprising a constriction portion that is a taper inclined toward the surface.
The inspection chip has a first surface and a second surface facing the first surface,
The width of the flow path inlet in the direction in which the first surface and the second surface face each other is smaller than the width in the direction in which the first surface and the second surface of the holding portion face each other. The inspection chip according to any one of 1 to 10.
The inspection chip has a first surface and a second surface facing the first surface,
An inlet side wall part that is a wall part extending from the holding part toward the flow path inlet;
A virtual direction that extends in parallel with the first surface and the second surface from the end of the injection port in the injection direction and extends perpendicular to the injection direction is a virtual direction,
Of the angles formed between the virtual direction and the inlet side wall, the angle formed between the end of the inlet in the injection direction and the flow path inlet is 90 degrees or less. The inspection chip according to claim 1.