WO2015080191A1

WO2015080191A1 - Inspection device, inspection method, and inspection program

Info

Publication number: WO2015080191A1
Application number: PCT/JP2014/081336
Authority: WO
Inventors: 由美子大鹿; 千里吉村
Original assignee: ブラザー工業株式会社
Priority date: 2013-11-29
Filing date: 2014-11-27
Publication date: 2015-06-04
Also published as: JP2015105889A

Abstract

This invention provides an inspection device, an inspection method, and an inspection program that make it possible to mix two different liquids into a homogenous mixture. This inspection device rotates an inspection chip that has a mixing section inside which an analyte is mixed with a reagent as a result of centrifugal force from said rotation acting on the analyte and the reagent. A CPU in the inspection device rotates (S75) the inspection chip at a given velocity of revolution (Vc), then stops (S77) the rotation of the inspection chip in the direction of revolution, then rotates (S79) the inspection chip at a different velocity of revolution (Ve), and then stops (S81) the rotation of the inspection chip in the direction of revolution again.

Description

Inspection device, inspection method, and inspection program

The present disclosure relates to an inspection apparatus, an inspection method, and an inspection program for performing a chemical, medical, or biological inspection of an inspection object.

Conventionally, an inspection apparatus for inspecting a specimen such as a biological substance or a chemical substance is known. For example, the microchip disclosed in Patent Document 1 includes a mixing unit having at least a first wall and a second wall. One end portions of the first wall and the second wall are connected to each other. The first wall and the second wall extend in different directions. A centrifugal force X in a direction perpendicular to the second wall and a centrifugal force Y in a direction perpendicular to the first wall are alternately applied to the microchip. When the centrifugal force X is applied, the two types of liquid introduced into the mixing unit are pressed against the inner wall surface of the second wall and stretched. Next, when the centrifugal force Y is applied, the two types of liquid stretched on the inner wall surface of the second wall once gather and contract at the connection portion between the first wall and the second wall, It is stretched to the inner wall surface of one wall. Thereby, two or more types of liquids are mixed.

Japanese Unexamined Patent Publication No. 2009-281779

In the microchip disclosed in Patent Document 1, two types of liquids are pressed against the inner wall surfaces of the first wall and the second wall by centrifugal force. Accordingly, the two types of liquids may be immobilized on the inner wall surfaces of the first wall and the second wall, and may be difficult to move. In this case, there is a problem that it is difficult to uniformly mix two kinds of liquids.

An object of the present disclosure is to provide an inspection apparatus, an inspection method, and an inspection program capable of uniformly mixing two kinds of liquids.

The inspection apparatus according to the first aspect of the present disclosure rotates the inspection chip including a mixing unit in which the sample and the reagent are mixed, and causes the centrifugal force generated by the rotation to act on the sample and the reagent. An inspection apparatus for mixing the reagent in the mixing unit, wherein the inspection chip is rotated, the inspection chip is rotated, the rotation is stopped, and the inspection chip is stopped from rotating. A control unit for rotating the chip is provided.

If the sample and reagent are mixed without stopping the rotation of the test chip, the sample and reagent spread in the direction perpendicular to the direction of the centrifugal force even if the direction of the centrifugal force changes. Hard to be done. On the other hand, in the first aspect, the inspection apparatus stops the inspection chip after rotating the inspection chip. As a result, the specimen and the reagent spreading in the direction orthogonal to the direction of the centrifugal force can spread in a direction orthogonal to the direction of gravity different from the direction of the centrifugal force. Furthermore, the inspection apparatus rotates the inspection chip again after stopping the rotation of the inspection chip. As a result, the specimen and the reagent that have spread in the direction perpendicular to the direction of gravity can spread in the direction perpendicular to the direction of the centrifugal force. Therefore, the specimen and the reagent can be mixed more uniformly by spreading in two directions perpendicular to the centrifugal force and two directions perpendicular to the gravity direction.

The control unit of the inspection apparatus rotates the inspection chip at a first rotation speed, stops the rotation after rotating the inspection chip at the first rotation speed, and stops the rotation of the inspection chip. Later, the inspection chip may be rotated at a second rotational speed different from the first rotational speed.

The control unit of the inspection apparatus may rotate the inspection chip at the second rotation speed that is slower than the first rotation speed after stopping the rotation of the inspection chip.

In the test apparatus, the test chip includes a sample quantification unit for quantifying the sample and a plurality of reagent quantification units for quantifying each of a plurality of reagents, and the control unit is quantified by the sample quantification unit. The test chip may be rotated after the sample and the plurality of reagents quantified by each of the plurality of reagent quantification units are injected into the mixing unit.

The test method according to the second aspect of the present disclosure includes rotating the test chip including a mixing unit in which the sample and the reagent are mixed, and causing the centrifugal force generated by the rotation to act on the sample and the reagent. An inspection method for mixing the reagent in the mixing unit, wherein the inspection chip is rotated, the inspection chip is rotated, the rotation is stopped, and the inspection chip is stopped from rotating. A control step for rotating the chip is provided. According to the 2nd aspect, there can exist an effect similar to a 1st aspect.

The test program according to the third aspect of the present disclosure rotates the test chip including a mixing unit in which the sample and the reagent are mixed, and causes the centrifugal force generated by the rotation to act on the sample and the reagent. After the inspection chip is rotated by the computer of the inspection apparatus that mixes the reagent in the mixing unit, the inspection chip is rotated, the rotation is stopped, and the rotation of the inspection chip is stopped, then the inspection A control step for rotating the chip is executed. According to the 3rd aspect, there can exist an effect similar to a 1st aspect.

1st aspect WHEREIN: The said test | inspection apparatus may be equipped with the holder which supports the said test | inspection chip along the extending direction of the flow path containing the said mixing part in the centrifugal direction by rotation of the said test | inspection chip along the gravity direction. . As a result, when the rotation of the test chip is stopped, the area in which the specimen and the reagent move in the direction of gravity increases, and the mixture can be mixed more uniformly.

In the first aspect, the control unit rotates the inspection chip in a state where the holder supports the inspection chip in a posture in which the opening direction of the mixing unit formed in a concave shape is opposite to the direction of gravity. After rotating at a speed and rotating the inspection chip, the rotation may be stopped, and after stopping the rotation of the inspection chip, the inspection chip may be rotated at a second rotation speed. Thereby, when the rotation of the test chip is stopped, it is possible to reduce the outflow of the specimen and the reagent moving along the direction of gravity from the mixing unit.

It is a figure which shows the structure of the test | inspection system 3 containing the test | inspection apparatus 1 and the control apparatus 90. FIG. It is the top view which looked at the test | inspection chip 2 from the front surface 201 side. It is the top view which looked at the test | inspection chip 2 from the rear surface 202 side. It is a flowchart of a centrifugation process. It is a flowchart of a centrifugation process, Comprising: FIG. 4 is a continuation. It is a state transition diagram of the test | inspection chip 2 in a centrifugation process. It is a state transition diagram of the test | inspection chip 2 in a centrifugation process. It is a state transition diagram of the test | inspection chip 2 in a centrifugation process. It is a state transition diagram of the test | inspection chip 2 in a centrifugation process.

Embodiments that embody the present disclosure will be described with reference to the drawings. 1 shows a plane of the inspection apparatus 1 constituting the inspection system 3 and functional blocks inside the control apparatus 90.

<1. Schematic structure of inspection system 3>
A schematic structure of the inspection system 3 will be described with reference to FIG. The inspection system 3 of the present embodiment includes an inspection chip 2 that can store a sample and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2. When the inspection device 1 rotates the inspection chip 2 around the vertical axis A 1 separated from the inspection chip 2, centrifugal force acts on the inspection chip 2. When the inspection apparatus 1 rotates the inspection chip 2 around the horizontal axis A2, the centrifugal direction, which is the direction of the centrifugal force acting on the inspection chip 2, is switched. Note that the inspection system 3 and the inspection apparatus 1 of the present embodiment have a known structure as described in Japanese Patent Application Laid-Open No. 2012-78107. Therefore, in the following description, an outline of the structure of the inspection apparatus 1 will be described. To do.

<2. Structure of the inspection apparatus 1>
The structure of the inspection apparatus 1 will be described with reference to FIG. In the following description, the upper side, the lower side, the right side, the left side, the front side of the paper surface, and the back side of the paper surface in FIG. 1 are defined as the front side, the rear side, the right side, the left side, the upper side, and the lower side, respectively. . In the present embodiment, the direction of the vertical axis A1 is the vertical direction of the inspection apparatus 1, and the direction of the horizontal axis A2 is the direction of the speed when the inspection chip 2 is rotated about the vertical axis A1. FIG. 1 shows a state where the top plate of the upper housing 30 of the inspection apparatus 1 is removed.

As shown in FIG. 1, the inspection apparatus 1 includes an upper housing 30, a lower housing 31, an upper plate 32, a turntable 33, an angle changing mechanism 34, and a control device 90. The turntable 33 is a disk rotatably provided on the upper side of an upper plate 32 described later. The inspection chip 2 is held above the turntable 33. The angle changing mechanism 34 is a drive mechanism provided on the turntable 33. The angle changing mechanism 34 rotates the inspection chip 2 around the horizontal axis A2. The upper housing 30 is fixed to an upper plate 32 described later, and a measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside. The control device 90 is a controller that controls various processes of the inspection device 1.

The schematic structure of the lower housing 31 will be described. The lower housing 31 has a box-shaped frame structure in which frame members are combined. An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 31. A drive mechanism that rotates the turntable 33 around the vertical axis A1 is provided in the lower housing 31 as follows.

A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side of the lower housing 31. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.

The main shaft 57 is rotatably held by a support member (not shown) provided immediately below the upper plate 32. A pulley 38 is fixed to the main shaft 57 below the support member. A belt 39 is stretched over the pulley 37 and the pulley 38. When the main shaft motor 35 rotates the shaft 36, the driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39, and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

A guide rail (not shown) extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31. A T-shaped plate (not shown) is movable in the vertical direction in the lower housing 31 along the guide rail.

The above-described main shaft 57 is a hollow cylindrical body. An inner shaft (not shown) is a shaft that can move in the vertical direction inside the main shaft 57. The upper end portion of the inner shaft passes through the main shaft 57 and is connected to the rack gear 43. A bearing (not shown) is provided at the left end of the T-shaped plate. Inside the bearing, the lower end portion of the inner shaft is rotatably held.

A stepping motor 51 for moving the T-shaped plate up and down is fixed in front of the T-shaped plate. The shaft 58 of the stepping motor 51 protrudes rearward, that is, downward in FIG. A disc-shaped cam plate (not shown) is fixed to the tip of the shaft 58. A cylindrical projection (not shown) is provided on the rear surface of the cam plate. The tip of the protrusion is inserted into a groove (not shown). The protrusion can slide in the groove. When the stepping motor 51 rotates the shaft 58, the protrusion moves up and down in conjunction with the rotation of the cam plate. At this time, the T-shaped plate moves up and down along the guide rail in conjunction with the protrusion inserted in the groove.

The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 (not shown) fixed to the inner shaft is provided between the pair of L-shaped plates 60. The rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.

A horizontal support shaft 46 having a gear 45 is rotatably supported at the distal end side in the extending direction of each L-shaped plate 60. The support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis (not shown) is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 is rotated about the support shaft 46.

In this embodiment, as the main shaft motor 35 rotates and drives the turntable 33, the inspection chip 2 rotates about the main shaft 57, which is a vertical axis, and a centrifugal force acts on the inspection chip 2. The rotation around the vertical axis A1 of the inspection chip 2 is called revolution, and the rotation direction of revolution is called the revolution direction. On the other hand, as the stepping motor 51 moves the inner shaft up and down, the inspection chip 2 rotates about the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively. The rotation of the inspection chip 2 around the horizontal axis A2 is called rotation, and the rotation direction of rotation is called the rotation direction.

When the T plate is lowered to the lowermost end of the movable range, the rack gear 43 is also lowered to the lowermost end of the movable range. At this time, the inspection chip 2 is in a steady state where the rotation angle is 0 degree. Further, in the state where the T-shaped plate is raised to the uppermost end of the movable range, the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the test | inspection chip 2 will be in the state rotated 180 degree | times centering on the horizontal axis line A2 from the steady state. That is, in this embodiment, the angular width that the test chip 2 can rotate is the rotation angle of 0 degrees to 180 degrees.

The detailed structure of the upper housing 30 will be described. As shown in FIG. 1, the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.

The measurement unit 7 provided in the upper housing 30 includes a light source 71 that emits measurement light, and an optical sensor 72 that detects the measurement light emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. In the present embodiment, the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light. When the inspection chip 2 is at the measurement position, the measurement light connecting the light source 71 and the optical sensor 72 intersects the front surface and the rear surface of the inspection chip 2 substantially perpendicularly.

<3. Electrical configuration of control device 90>
The electrical configuration of the control device 90 will be described with reference to FIG. The control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for a user to input instructions to the control device 90, a hard disk device 95 for storing various data and programs, and a display 96 for displaying various information. As the control device 90, a personal computer may be used, or a dedicated control device may be used.

Further, a revolution controller 97, a rotation controller 98, and a measurement controller 99 are connected to the CPU 91. The revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35. The rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51. The measurement controller 99 performs optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the optical sensor 72 to the light source 71 and the optical sensor 72. The CPU 91 controls the revolution controller 97, the rotation controller 98, and the measurement controller 99.

<4. Structure of inspection chip 2>
With reference to FIG.2 and FIG.3, the detailed structure of the test | inspection chip 2 which concerns on this embodiment is demonstrated. In the following description, the upper, lower, left, right, front side and back side of FIG. 2 are the upper, lower, left, right, front, and rear sides of the inspection chip 2, respectively. .

As shown in FIG. 2 and FIG. 3, the inspection chip 2 has a square shape when viewed from the front as an example, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness. As shown in FIG. 2, the front surface 201 of the plate member 20 is sealed with a sheet 291 made of a transparent synthetic resin thin plate. As shown in FIG. 3, the rear surface 202 opposite to the front surface 201 is sealed with a sheet 292 made of a transparent synthetic resin thin plate. As shown in FIGS. 2 and 3, a liquid flow path 25 is formed between the plate material 20 and the sheet 291 and between the plate material 20 and the sheet 292 so that the liquid sealed in the inspection chip 2 can flow. Has been. The liquid channel 25 is a recess formed at a predetermined depth on the front surface 201 side and the rear surface 202 side of the plate material 20, and extends in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate material 20. The

sheets

291 and 292 seal the flow path forming surface of the plate material 20. The

sheets

291 and 292 are not shown except for FIGS.

The liquid channel 25 includes the sample quantification channel 11, the

reagent quantification channels

13 and 15, the mixing unit 80, and the measurement unit 81. The reagent fixed amount flow path 13 includes a first connection flow path 301. The reagent fixed amount flow path 15 includes a second connection flow path 331. As shown in FIG. 2, the mixing unit 80 is provided in the lower right portion of the front surface 201. The reagent fixed amount flow path 13 extends from the upper left part on the front surface 201 toward the mixing unit 80. The sample fixed amount flow path 11 extends from the upper right part on the front surface 201 toward the mixing part 80. As shown in FIG. 3, the reagent fixed amount flow path 15 extends from the upper left part on the rear surface 202 side toward the mixing unit 80. The mixing unit 80 is an area that includes a channel on the right side of an end 315 (described later) and an inlet 306 (described later) that is connected to a channel 117 (described later) and extends downward. The measurement unit 81 is a lower part of the mixing unit 80.

The configuration common to the

reagent quantification channels

13 and 15 will be described. As shown in FIGS. 2 and 3, the

reagent quantification channels

13 and 15 include the inlet 130, the reagent holding unit 131, the supply unit 132, the reagent quantification unit 134, the passage 137, the guide unit 139, and the surplus unit 136, respectively. including. The reagent holding part 131 is provided in the upper left part of the test chip 2. The reagent holding part 131 is a recess that opens upward. The injection port 130 penetrates the plate member 20 from the upper part of the reagent holding part 131 toward the upper side part 21 of the test chip 2. The inlet 130 is a part where the first reagent 18 or the second reagent 19 is injected into the reagent holding part 131. The reagent holding part 131 of the reagent fixed amount flow channel 13 is a part where the first reagent 18 injected from the injection port 130 of the reagent fixed amount flow channel 13 is stored. The reagent holding part 131 of the reagent fixed amount flow channel 15 is a part where the second reagent 19 injected from the injection port 130 of the reagent fixed amount flow channel 15 is stored. In the following description, the first reagent 18 and the second reagent 19 are collectively referred to as “reagent 16” when not specified either.

2 and 3, the supply unit 132 is a flow channel extending downward from the upper right part of the reagent holding unit 131. The lower end part of the supply part 132 is connected to the guide part 139 which is a passage having a narrow channel. A reagent quantitative unit 134 is provided below the guide unit 139. The guide unit 139 opens toward the reagent quantitative unit 134. The reagent quantification part 134 is a part where the reagent 16 is quantified, and is a concave part recessed in the lower left.

The reagent quantification unit 134 is connected to the mixing unit 80 through the first connection channel 301 and is connected to the surplus unit 136 through the passage 137. The right end portion that is one end portion on the mixing unit 80 side of the reagent fixed amount unit 134 is referred to as a first end portion 141. The first end 141 of the reagent quantification unit 134 on the front surface 201 side communicates with a first connection channel 301 described later. The first end 141 of the reagent quantification unit 134 on the rear surface 202 side communicates with the second connection channel 331. The left end of the reagent quantification unit 134 opposite to the mixing unit 80 is referred to as a second end 142. That is, the passage 137 extends from the second end portion 142 toward the surplus portion 136. A surface connecting the first end portion 141 and the second end portion 142 is a fixed amount surface 146. The fixed amount surface 146 is a virtual surface that is the position of the upper surface of the reagent 16 when the reagent 16 is quantified by the reagent fixed amount unit 134. Therefore, the volume of the liquid flow path 25 below the fixed amount surface 146 is the fixed amount in the reagent fixed amount unit 134. A straight line extending from the second end 142 of the reagent quantitative unit 134 in a direction orthogonal to the quantitative surface 146 is defined as a virtual line 142A. Guide part 139 is arranged on virtual line 142A. The capacity of the reagent quantification unit 134 is larger than the capacity of the sample quantification unit 114 described later.

The passage 137 extends obliquely downward to the left from the upper part of the reagent quantitative unit 134. The passage 137 is a channel through which the reagent 16 overflowing from the reagent quantitative unit 134 moves. A surplus part 136 is provided at the lower left of the reagent quantitative unit 134. The surplus portion 136 is a portion in which the reagent 16 that has moved through the passage 137 is accommodated, and is a recess provided in the downward direction and the right direction from the lower end portion of the passage 137.

The first connection channel 301 will be described. In the following description, the reagent quantitative unit 134 of the reagent quantitative channel 13 is referred to as a reagent quantitative unit 134A, and the reagent quantitative unit 134 of the reagent quantitative channel 15 is referred to as a reagent quantitative unit 134B. The first connection channel 301 is a channel that is formed on the front surface 201 and connects the reagent quantitative unit 134A and the mixing unit 80. The first connection channel 301 extends obliquely upward to the right from the upper part of the reagent quantitative unit 134A, extends downward from the right end part, and further extends to the right from the lower end part. The first connection channel 301 is formed by a first wall surface 302 and a second wall surface 303. The first wall surface 302 is a wall surface facing the reagent quantitative unit 134A and extending toward the mixing unit 80 side. The first wall surface 302 extends from the lower end of the guide portion 139 to a right end portion 314 that forms an inflow port 306 described later. The second wall surface 303 is a wall portion facing the first wall surface 302. The second wall surface 303 extends from the first end 141 of the reagent quantitative unit 134A to the right end 313 that forms an inflow port 306 described later.

The first connection channel 301 includes a partial receiving part 304, a reagent receiving part 305, and an inflow port 306. The partial receiving unit 304 is a part that holds a part of the first reagent 18 quantified by the reagent quantification unit 134A. The partial receiver 304 is provided on the right side of the reagent quantitative unit 134A and on the reagent quantitative unit 134A side from a merging hole 351 described later. The partial receiving portion 304 is a concave portion that opens in the left direction from the first end portion 141 toward the second end portion 142. The capacity of the partial receiving unit 304 is smaller than the capacity of the reagent quantitative unit 134A.

Among the second wall surfaces 303, the wall surface connected to the first end portion 141 of the reagent quantitative unit 134 A is referred to as a reagent flow channel wall surface 308. That is, the reagent channel wall surface 308 is connected to the reagent quantitative unit 134A in the first connection channel 301 and forms a part of the first connection channel 301. The reagent channel wall surface 308 extends obliquely upward to the right from the first end portion 141, bends slightly upward at the bent portion 309, and extends obliquely upward to the right. The imaginary line 311 drawn in the direction perpendicular to the reagent channel wall surface 308 from the end 310 on the mixing channel 80 side in the reagent channel wall surface 308 is the first connection channel 301 on the reagent quantitative unit 134A side from the partial receiver 304. Intersects with

The reagent receiving unit 305 is provided below the partial receiving unit 304 between the partial receiving unit 304 and the mixing unit 80. The reagent receiving part 305 is a concave part having an upper opening on the partial receiving part 304 side, and is a part for holding the first reagent 18 that moves downward after being held by the partial receiving part 304. The reagent receiving part 305 is formed by the wall surface 303A, the wall surface 303B, and the wall surface 303C of the second wall surface 303. The wall surface 303 A is a wall surface extending vertically to the right of the surplus portion 136 of the reagent fixed amount flow path 13. The wall surface 303B is a wall surface extending in the right direction from the lower end of the wall surface 303A. The right end portion of the wall surface 303 B is located on the lower left side of the mixing unit 80. The wall surface 303C is a wall surface extending obliquely upward to the right from the right end portion of the wall surface 303B.

The inflow port 306 is formed by a right end 313 of the wall surface 303C and a right end 314 of the first wall 302 located above the right end 313. The inflow port 306 is located on the upper left side of the mixing unit 80 and is a part through which the reagent 16 flows into the mixing unit 80.

A confluence hole portion 351 is provided at the center in the left-right direction at the lower end of the first connection channel 301. The merge hole 351 is a hole that penetrates the plate member 20 in the front-rear direction and joins the second connection channel 331 to the first connection channel 301. Of the lower end portion 352 on the second wall surface 303 side in the joining hole portion 351 and the upper end portion 353 facing the end portion 352, the end portion 352 is left and right along the wall surface 303 B of the second wall surface 303. Extend in the direction. The end portion 353 is along the first wall surface 302.

The second connection channel 331 will be described. As shown in FIG. 3, the second connection channel 331 is a channel that is formed on the rear surface 202 and extends from the reagent quantitative unit 134 B toward the mixing unit 80 and connects the reagent quantitative unit 134 B and the mixing unit 80. The second connection channel 331 includes four

reagent receiving portions

341, 342, 343, and 344. The reagent receiving units 341 to 344 receive the second reagent 19 quantified by the reagent quantifying unit 134B. The reagent receiving part 341 is a concave part that is located on the right side of the reagent fixed quantity part 134B and opens to the left. The reagent receiving part 342 is a recessed part that is located on the lower left side of the reagent receiving part 341 and opens upward. The reagent receiving part 343 is a recessed part that is located on the lower right side of the reagent receiving part 342 and opens to the left. The reagent receiving part 344 is a recessed part that is located below the reagent receiving part 343 and opens upward.

The second connection channel 331 extends obliquely upward and to the right from the reagent determination unit 134B and connects to the reagent receiver 341, and extends downward and obliquely to the left from the reagent receiver 341 and connects to the reagent receiver 342. The second connection channel 331 extends obliquely upward to the right from the reagent receiving part 342, extends downward from the right end, and is connected to the reagent receiving part 343. The second connection channel 331 extends obliquely downward to the left from the reagent receiving part 343 and is connected to the reagent receiving part 344. The right end portion of the reagent receiving portion 344 is connected to the merge hole portion 351 and is connected to the first connection flow path 301 on the front surface 201 side.

The specimen quantification channel 11 will be described. As shown in FIG. 2, the sample fixed amount flow path 11 includes an injection port 110, a sample holding unit 111, a first supply unit 112, a first guide unit 113, a separation unit 124, a passage 125, a passage 127, and a first surplus portion. 126, the second supply unit 123, the sample determination unit 114, the passage 115, the passage 117, and the second surplus unit 116. The sample holding unit 111 is provided on the right side of the supply unit 132 of the reagent fixed amount flow channel 13. The sample holder 111 is a recess that opens upward. The injection port 110 penetrates the plate member 20 from the upper part of the specimen holding part 111 toward the upper side part 21 of the test chip 2. The injection port 110 is a part where the sample 17 is injected into the sample holding unit 111. The sample holding unit 111 is a part where the sample 17 injected from the injection port 110 is stored. The specimen 17 of the present embodiment is a liquid containing components such as blood, plasma, blood cells, bone marrow, urine, vaginal tissue, epithelial tissue, tumor, semen, saliva, or foodstuff. The first supply unit 112 is a flow path that extends downward from the upper right portion of the specimen holding unit 111. The lower end part of the 1st supply part 112 is connected with the 1st guide part 113 which is a channel | path with which the flow path was narrowly formed.

A separation unit 124 is provided below the first guide unit 113. The first guide unit 113 guides the sample 17 to the separation unit 124. The separation unit 124 is a part where components contained in the specimen 17 are separated. The separation part 124 is a recess that opens upward and tilts diagonally downward to the right. The separation unit 124 centrifuges the specimen 17 into a component having a small specific gravity and a component having a large specific gravity by the action of centrifugal force. In the following description, as shown in FIG. 7C, a component having a small specific gravity of the sample 17 separated in the separation unit 124 is referred to as a sample 17A, and a component having a large specific gravity is referred to as a sample 17B.

The connection channel 120 extends obliquely upward to the right from the central portion in the vertical direction on the right side surface of the separation unit 124, and the upper end of the connection channel 120 is connected to the upper end of the component holding unit 121. The component holding unit 121 is a storage unit that holds the sample 17A and a part of the sample 17B separated by the separation unit 124. Further, the width of the flow path of the connection flow path 120 is narrower than the width of the flow path of the passage 127 described later. Therefore, the specimen 17A flows out to the passage 127 before flowing into the connection channel 120. Therefore, the possibility that the sample 17A flows into the component holding unit 121 before the passage 127 can be reduced.

From the upper part of the separation part 124, the passage 125 extends obliquely to the left and the passage 127 extends obliquely upward to the right. The passage 125 extends to the first surplus portion 126 provided on the lower left side of the separation portion 124. The first surplus portion 126 is a portion where the specimen 17 overflowing from the separation portion 124 is stored, and is a recess provided in the right direction and the downward direction from the lower end portion of the passage 125.

The passage 127 is connected to the second supply unit 123. The second supply unit 123 is a flow path that extends downward from the upper right portion of the passage 127. The lower end of the 2nd supply part 123 is connected with the 2nd guide part 128 which is a channel | path with which the flow path was narrowly formed. Below the second guide unit 128, a sample quantitative unit 114 is provided. The second guide unit 128 opens toward the sample quantitative unit 114. The sample quantification unit 114 is a part that quantifies the sample 17A, and is a recess that opens upward. The sample quantification unit 114 is recessed obliquely downward to the left.

The specimen quantification unit 114 is connected to the mixing unit 80 through the passage 117 and is connected to the second surplus unit 116 through the passage 115. The right end portion, which is one end portion on the mixing unit 80 side of the sample determination unit 114, is referred to as a first end portion 114A. The first end 114A communicates with a passage 117 described later. The left end portion of the sample quantification unit 114 opposite to the mixing unit 80 is referred to as a second end portion 114B. That is, the passage 115 extends from the second end portion 114 B toward the second surplus portion 116. A surface connecting the first end portion 114A and the second end portion 114B of the sample quantification unit 114 is a quantification surface 114C. The fixed surface 114C is a virtual surface that is the position of the upper surface of the sample 17A when the sample 17A is quantified by the sample quantitative unit 114. Therefore, the volume of the liquid flow path 25 below the fixed surface 114C is the fixed amount in the sample fixed portion 114. A straight line extending from the first end 114A of the sample quantitative unit 114 in a direction orthogonal to the quantitative surface 114C is defined as a virtual line 114D. The second guide part 128 is disposed on the virtual line 114D. The capacity of the sample quantification unit 114 is smaller than the capacity of the reagent quantification unit 134.

From the upper part of the specimen quantification unit 114, the passage 115 extends obliquely to the left and the passage 117 extends obliquely upward to the right. A second surplus part 116 is provided at the lower left of the sample quantification part 114. The passage 115 is connected to the second surplus portion 116. The second surplus part 116 is a part where the specimen 17A overflowing from the specimen quantification part 114 is stored. The second surplus portion 116 is a recess provided in the right direction from the lower end portion of the passage 115. The passage 117 is connected to the mixing unit 80.

The mixing unit 80 extends downward on the right side of the end 315 and the inlet 306. The mixing unit 80 is connected to the sample quantifying unit 114 via the passage 117. The mixing unit 80 is connected to the reagent quantitative unit 134A via the first connection channel 301. The mixing unit 80 is connected to the reagent quantification unit 134B via the second connection channel 331. Of the inner wall surfaces of the mixing unit 80, the right wall surface 80A extends diagonally downward to the right from the upper end, bends downward and extends downward, bends to the left, and extends diagonally downward to the left. Of the inner wall surface of the mixing unit 80, the lower wall surface 80B is curved. In the mixing unit 80, the sample 17A quantified in the sample quantification unit 114, the first reagent 18 quantified in the reagent quantification unit 134A, and the second reagent 19 quantified in the reagent quantification unit 134B are mixed. When optical measurement described later is performed, the measurement light is transmitted to the measurement unit 81 that forms the lower part of the mixing unit 80.

<5. Other structures of inspection chip 2>
As shown in FIG. 1, the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates around the support shaft 46. When the inspection chip 2 is in the steady state shown in FIGS. 2 and 3, the upper side 21 and the lower side 24 are orthogonal to the direction of gravity G, the right side 22 and the left side 23 are parallel to the direction of gravity G, and The left side portion 23 is disposed closer to the main shaft 57 than the right side portion 22. In a state where the inspection chip 2 in the steady state is arranged at the measurement position, the inspection apparatus 1 performs inspection by optical measurement by allowing the measurement light connecting the light source 71 and the optical sensor 72 to pass through the measurement unit 81.

<6. Example of inspection method>
An inspection method using the inspection apparatus 1 and the inspection chip 2 will be described. As shown in FIG. 2, the sample 17 is injected from the injection port 110 and placed in the sample holding unit 111. The first reagent 18 is injected from the inlet 130 of the reagent quantitative flow path 13 and is disposed in the reagent holding part 131 of the reagent quantitative flow path 13. As shown in FIG. 3, the second reagent 19 is injected from the inlet 130 of the reagent quantitative channel 15 and is arranged in the reagent holding part 131 of the reagent quantitative channel 15. The arrangement method of the first reagent 18, the second reagent 19, and the specimen 17 is not limited. For example, holes are opened at positions corresponding to the sample holding unit 111 and the reagent holding unit 131 in the

sheets

291 and 292, and the user injects the sample 17, the first reagent 18, and the second reagent 19 from the holes. You may seal and seal. In addition, the first reagent 18 and the second reagent 19 may be disposed in advance in the reagent holding portions 131 of the reagent

quantitative flow paths

13 and 15 and sealed with

sheets

291 and 292 in advance. In this case, a hole may be opened in the sheet 291 at a position corresponding to the sample holding portion 111 of the sample fixed amount flow path 11, and the user may inject the sample 17 from the hole, and further seal and seal.

When the user attaches the inspection chip 2 to a mounting holder (not shown) and inputs a processing start command from the operation unit 94, the CPU 91 is shown in FIGS. 4 and 5 based on a control program stored in the ROM 93. Centrifugation is performed. Although the inspection apparatus 1 can inspect two inspection chips 2 simultaneously, a procedure for inspecting one inspection chip 2 will be described below for convenience of explanation.

In the following description, the steady state of the inspection chip 2 shown in FIG. 2 and FIG. 3 is referred to as a rotation angle of 0 degree, and the states rotated counterclockwise by 40 degrees, 85 degrees, and 90 degrees from the steady state are respectively rotated. The angle is 40 degrees, the rotation angle is 85 degrees, and the rotation angle is 90 degrees. The CPU 91 shown in FIG. 1 controls the revolution controller 97 so that the spindle motor 35 rotates the turntable 33 based on an instruction from the revolution controller 97, and the CPU 91 rotates the turntable 33. By the rotation of the turntable 33, the inspection chip 2 rotates in the revolution direction. When the CPU 91 controls the rotation controller 98, the stepping motor 51 rotates the inspection chip 2 based on an instruction from the rotation controller 98, and the CPU 91 rotates the inspection chip 2. By the rotation of the stepping motor 51, the inspection chip 2 rotates in the rotation direction.

The rotation speed of the inspection chip 2 that rotates in the revolving direction by the rotation of the turntable 33 is called a revolving speed, and is represented by V0, Vg, Vf, Vd, Ve, Vc, and Vm. Each unit of V0, Vg, Vf, Vd, Ve, Vc, and Vm is rpm. V0 is 0 rpm. A relationship of V0 <Vg <Vf <Vd <Ve <Vc <Vm is established among V0, Vg, Vf, Vd, Ve, Vc, and Vm. For example, Vg may be 10 rpm, Vf may be 100 rpm, Vd may be 500 rpm, Ve may be 1000 rpm, Vc may be 2000 rpm, and Vm may be 4000 rpm. For example, Ve and Vc may be the same, or Ve may be larger than Vc. Moreover, the rotation speed of the test | inspection chip 2 rotated in the autorotation direction by rotation of the stepping motor 51 is called autorotation speed, and is represented by V0, VA, VB, VC, VD. Each unit of VA to VD is rpm. A relationship of VA> VB> VC> VD> V0 is established between V0 and VA to VD.

<6-1. First step>
As shown in FIG. 4, the CPU 91 reads motor drive information stored in advance in the HDD 95, sets drive information of the spindle motor 35 in the revolution controller 97, and sets drive information of the stepping motor 51 in the rotation controller 98. (S11). The state of the inspection chip 2 is a rotation angle of 0 degrees as shown in FIGS. Next, the CPU 91 starts the revolution of the inspection chip 2 by starting the rotation of the turntable 33 (S13). The centrifugal force X in the direction from the left side 23 to the right side 22 of the test chip 2 shown in FIGS. 2 and 3 starts to act on the reagent 16 and the sample 17. As a result, the reagent 16 starts to flow from the reagent holding unit 131 to the supply unit 132, and the sample 17 starts to flow from the sample holding unit 111 to the first supply unit 112. Next, the CPU 91 increases the revolution speed from V0 to Vm (S15). For this reason, centrifugal force becomes large. By increasing the revolution speed from V0 to Vm, the CPU 91 can cause the sample 17 in the sample holding unit 111 to flow to the first supply unit 112 without remaining in the sample holding unit 111. Next, the CPU 91 increases the revolution speed to Vm and then holds the revolution speed at Vm (S15).

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S17). For this reason, centrifugal force becomes small. Next, after reducing the revolution speed to Vc, the CPU 91 holds the revolution speed at Vc (S17). The CPU 91 sends the sample 17 to the first supply unit 112 with the revolution speed set to Vm so that the sample holding unit 111 does not leave the liquid. However, when the revolution speed is set to Vm and the state of the test chip 2 is changed from 0 degree to 90 degrees in the rotation of the test chip 2 in the process of S19 described later, a strong centrifugal force acts on the reagent 16. For this reason, when the reagent 16 is injected from the supply unit 132 with respect to the reagent 16 injected into the reagent quantification unit 134, the reagent 16 injected into the reagent quantification unit 134 is scattered, and the quantification accuracy may be lowered. There is sex. For this reason, before changing the state of the test | inspection chip 2 from 0 degree of rotation angles to 90 degree | times in the process of S19 mentioned later, CPU61 reduces revolution speed from Vm to Vc, and makes centrifugal force small. Thereby, scattering of the reagent 16 injected into the reagent quantitative unit 134 can be reduced.

After the processing of S17 is executed, the reagent 16 that has flowed out of the reagent holding unit 131 is stored in the supply unit 132 as shown in FIG. The sample 17 flowing out from the sample holding unit 111 is stored in the first supply unit 112.

<6-2. Second step>
The CPU 91 rotates the inspection chip 2 with the rotation speed set to VA while maintaining the revolution speed at Vc, and changes the state of the inspection chip 2 from 0 degree to 90 degrees (S19). The centrifugal direction of the centrifugal force X changes from the direction from the left side 23 to the right side 22 of the test chip 2 shown in FIGS. 2 and 3 to the direction from the upper side 21 to the lower side 24. As a result, the reagent 16 starts to flow from the supply unit 132 to the reagent quantitative unit 134 via the guide unit 139, and the reagent 16 is injected into the reagent quantitative unit 134. The reagent 16 overflowing from the reagent quantitative unit 134 flows to the surplus unit 136 via the passage 137. In addition, the sample 17 starts to flow from the first supply unit 112 to the separation unit 124 via the first guide unit 113, and the sample 17 is injected into the separation unit 124. The specimen 17 overflowing from the separation part 124 flows to the first surplus part 126 via the passage 125.

After the process of S19 is executed, as shown in FIG. 6B, the centrifugal direction of the centrifugal force X is orthogonal to the fixed surface 146 and parallel to the virtual line 142A shown in FIG. When the centrifugal force X acts in the direction orthogonal to the quantification surface 146, the reagent 16 corresponding to the volume of the reagent quantification unit 134 is quantified. The capacity of the reagent quantification unit 134 is the capacity of the liquid channel 25 below the quantification surface 146. That is, when the centrifugal direction of the centrifugal force X is directed from the upper side 21 toward the lower side 24, the volume of the reagent 16 injected into the reagent quantitative unit 134 can be made the volume of the reagent quantitative unit 134. Further, the sample 17 corresponding to the volume of the separation unit 124 remains in the separation unit 124. The capacity of the separation part 124 is the capacity of the liquid flow path 25 below the virtual surface 148 extending in the horizontal direction from the end 147 on the passage 125 side of the separation part 124 shown in FIG.

Next, the CPU 91 increases the revolution speed from Vc to Vm (S21). For this reason, centrifugal force becomes large. The CPU 61 separates the specimen 17 injected into the separation unit 124 by increasing the revolution speed to Vm. Next, the CPU 91 increases the revolution speed to Vm and then holds the revolution speed at Vm (S21).

After the process of S21 is executed, the component of the specimen 17 is separated in the separation unit 124 as shown in FIG. For example, when the specimen 17 is blood, blood cells having a large specific gravity accumulate on the side in which the centrifugal force X acts, and plasma having a small specific gravity accumulates on the side opposite to the direction in which the centrifugal force X acts. That is, blood cells and plasma in the blood are separated. In the following description, a light component with a low specific gravity separated by the separation unit 124 is referred to as a sample 17A, and a component with a high specific gravity is referred to as a sample 17B.

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S23). For this reason, centrifugal force becomes small. Next, the CPU 91 reduces the revolution speed to Vc, and then holds the revolution speed at Vc (S23). When the revolution speed is set to Vm, when the state of the inspection chip 2 is changed from 90 degrees to 0 degrees in the process of S25 described later, the spindle motor 35 that supplies the driving force for rotating the turntable 33. The load on is increased. Therefore, the CPU 61 reduces the revolution speed from Vm to Vc before changing the state of the test chip 2 from 90 degrees to 0 degrees in the process of S25 described later in order to reduce the load on the spindle motor 35. .

<6-3. Third step>
The CPU 91 rotates the inspection chip 2 with the rotation speed set to VB, and rotates the state of the inspection chip 2 from the rotation angle of 90 degrees to 0 degrees (S25). The centrifugal direction changes from the direction from the upper side 21 to the lower side 24 of the test chip 2 shown in FIGS. 2 and 3 to the direction from the left side 23 to the right side 22. As a result, the first reagent 18 starts to flow from the reagent quantitative unit 134A to the partial receiver 304 side. Since the partial receiving unit 304 has a smaller capacity than the reagent quantitative unit 134A, a part of the first reagent 18 is stored in the partial receiving unit 304 and the rest overflows from the partial receiving unit 304. The first reagent 18 overflowing from the partial receiving portion 304 flows to the mixing portion 80 via the first wall surface 302, the wall surface 303C, and the inlet 306. In addition, the second reagent 19 starts to flow from the reagent quantitative unit 134B to the reagent receiving unit 341 side. Part of the sample 17A starts to flow from the separation unit 124 to the second supply unit 123 side. The sample 17A and the sample 17B remaining in the separation unit 124 start to flow toward the component holding unit 121 via the connection channel 120.

After the process of S25 is executed, the first reagent 18 is stored in the partial receiver 304 as shown in FIG. The first reagent 18 overflowing from the partial receiving unit 304 is stored in the mixing unit 80. The second reagent 19 is stored in the reagent receiver 341. A part of the specimen 17A is stored in the second supply unit 123. The sample 17A and the sample 17B remaining in the separation unit 124 are stored in the component holding unit 121.

Next, the CPU 91 increases the revolution speed from Vc to Vm (S27). For this reason, centrifugal force becomes large. Next, the CPU 91 increases the revolution speed to Vm and then holds the revolution speed at Vm (S27). By increasing the revolution speed from Vc to Vm, the CPU 91 can cause the first reagent 18 to flow to the partial receiving unit 304 without causing the reagent quantification unit 134A to remain liquid. Further, the CPU 91 can cause the second reagent 19 to flow to the reagent receiving unit 341 without causing the reagent quantification unit 134B to leave liquid.

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S29). For this reason, centrifugal force becomes small. Next, after reducing the revolution speed to Vc, the CPU 91 holds the revolution speed at Vc (S29). When the revolution speed is Vm and the state of the test chip 2 is changed from 0 degree to 85 degrees in the rotation of the test chip 2 in the process of S31 described later, a strong centrifugal force acts on the specimen 17A. For this reason, when the sample 17A is injected from the second supply unit 123 with respect to the sample 17A injected into the sample quantifying unit 114, the sample 17A injected into the sample quantifying unit 114 scatters, and the quantification accuracy decreases. there's a possibility that. Therefore, the CPU 61 reduces the revolution speed from Vm to Vc before changing the state of the test chip 2 from 0 degree to 85 degrees in the process of S31 described later. Thereby, scattering of the specimen 17A injected into the specimen quantification unit 114 can be reduced.

<6-4. Fourth step>
The CPU 91 rotates the inspection chip 2 with the rotation speed held at Vc while keeping the revolution speed at Vc, and changes the state of the inspection chip 2 from 0 degree to 85 degrees (S31). The centrifugal direction changes from the direction from the left side 23 to the right side 22 of the test chip 2 shown in FIGS. 2 and 3 to the direction inclined toward the right side 22 with respect to the direction from the upper side 21 to the lower side 24. To do. Thereby, the sample 17A stored in the second supply unit 123 starts to flow to the sample determination unit 114 via the second guide unit 128. Further, the first reagent 18 stored in the partial receiver 304 starts to flow toward the reagent receiver 305. The second reagent 19 stored in the reagent receiver 341 starts to flow toward the reagent receiver 342.

Next, the CPU 91 reduces the revolution speed from Vc to Vd (S33). For this reason, centrifugal force becomes small. Next, after reducing the revolution speed to Vd, the CPU 91 holds the revolution speed at Vd (S33). Even if the CPU 91 reduces the revolution speed from Vc to Vd after changing the state of the test chip 2 to the rotation angle of 90 degrees by the process of S35 described later, there is a possibility that the specimen 17A is not already left in the second supply unit 123. There is. Therefore, the CPU 91 decreases the revolution speed from Vc to Vd before changing the rotation angle to 90 degrees.

Next, the CPU 91 rotates the inspection chip 2 with the rotation speed as VD, and changes the state of the inspection chip 2 from a rotation angle of 85 degrees to 90 degrees (S35). The centrifugal direction changes from a direction inclined toward the right side 22 to a direction from the upper side 21 to the lower side 24 with respect to the direction from the upper side 21 to the lower side 24 of the test chip 2 shown in FIGS. To do. As a result, the sample 17A stored in the second supply unit 123 further flows into the sample determination unit 114. In addition, the specimen 17A overflowing from the specimen quantification unit 114 flows to the second surplus part 116. The first reagent 18 further flows to the reagent receiving unit 305 side. The second reagent 19 further flows to the reagent receiving part 342 side.

After the process of S35 is executed, as shown in FIG. 7E, the centrifugal direction of the centrifugal force X is orthogonal to the quantitative surface 114C and parallel to the virtual line 114D shown in FIG. The centrifugal force X acts in a direction perpendicular to the quantification surface 114C, whereby the sample 17A corresponding to the volume of the sample quantification unit 114 is quantified. The capacity of the specimen quantification unit 114 is the capacity of the liquid channel 25 below the quantification surface 114C. The first reagent 18 is stored in the reagent receiving unit 305. The second reagent 19 is stored in the reagent receiving part 342.

Next, the CPU 91 increases the revolution speed from Vd to Vm (S37). For this reason, centrifugal force becomes large. After increasing the revolution speed to Vm, the CPU 91 holds the revolution speed at Vm (S37). When the revolution speed is not increased from Vd to Vm, the centrifugal force acting on the specimen 17A is weak, and thus the specimen 17A may remain on the wall surface of the second guide portion 128. For this reason, the CPU 91 reduces the specimen 17A remaining on the wall surface of the second guide portion 128 by increasing the revolution speed to Vm.

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S39). For this reason, centrifugal force becomes small. After reducing the revolution speed to Vc, the CPU 91 holds the revolution speed at Vc (S39). The CPU 91 reduces the revolution speed to Vc in order to change the state of the test chip 2 from the rotation angle of 90 degrees to 40 degrees in the process of S41 to be described later and to send the specimen 17A from the specimen quantitative section 114 to the mixing section 80. Let

<6-5. Fifth process>
The CPU 91 rotates the inspection chip 2 with the rotation speed set to VB, and changes the state of the inspection chip 2 from a rotation angle of 90 degrees to 40 degrees (S41). As a result, the centrifugal direction of the test chip 2 shown in FIGS. 2 and 3 is from the direction from the upper side 21 to the lower side 24 to the direction from the left side 23 to the right side 22 toward the upper side 21. It changes to the inclined direction. Thereby, the sample 17A stored in the sample quantification unit 114 starts to flow from the sample quantification unit 114 to the mixing unit 80 side.

Next, the CPU 91 increases the revolution speed from Vc to Vm (S43). For this reason, centrifugal force becomes large. After increasing the revolution speed to Vm, the CPU 91 holds the revolution speed at Vm (S43). The CPU 91 can flow the sample 17A to the mixing unit 80 without causing the sample quantification unit 114 to remain liquid by increasing the revolution speed from Vc to Vm.

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S45). For this reason, centrifugal force becomes small. After reducing the revolution speed to Vc, the CPU 91 holds the revolution speed at Vc (S45). When the revolution speed is set to Vm, when the state of the inspection chip 2 is changed from the rotation angle 40 degrees to 0 degrees in the process of S47 described later, the spindle motor 35 that supplies the driving force for rotating the turntable 33. The load on is increased. Accordingly, the CPU 91 reduces the revolution speed from Vm to Vc before changing the state of the test chip 2 from the rotation angle of 40 degrees to 0 degrees in the process of S47 described later in order to reduce the load on the spindle motor 35.

Next, the CPU 91 rotates the inspection chip 2 with the rotation speed being VB, and changes the state of the inspection chip 2 from a rotation angle of 40 degrees to 0 degrees (S47). Thereby, the centrifugal direction changes from a direction inclined toward the upper side portion 21 to a direction from the left side portion 23 toward the right side portion 22 to a direction toward the right side portion 22 from the left side portion 23. Thereby, the first reagent 18 stored in the reagent receiving unit 305 starts to flow from the reagent receiving unit 305 to the mixing unit 80 side. After the process of S47 is executed, the first mixed liquid 261 is stored in the mixing unit 80 as shown in FIG. The second reagent 19 is stored in the reagent receiving unit 343.

Next, the CPU 91 increases the revolution speed from Vc to Vm (S49). For this reason, centrifugal force becomes large. After increasing the revolution speed to Vm, the CPU 91 holds the revolution speed at Vm (S49). By increasing the revolution speed from Vc to Vm, the CPU 91 can cause the first reagent 18 to flow through the mixing unit 80 without remaining in the reagent receiving unit 305.

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S51). For this reason, centrifugal force becomes small. After reducing the revolution speed to Vc, the CPU 91 holds the revolution speed at Vc (S51). When the revolution speed is set to Vm, when the state of the inspection chip 2 is changed from 0 degree to 90 degrees in the rotation angle in the process of S53 described later, the spindle motor 35 that supplies a driving force for rotating the turntable 33. The load on is increased. Accordingly, the CPU 91 reduces the revolution speed from Vm to Vc before changing the state of the inspection chip 2 from 0 degree to 90 degrees in the process of S53 described later in order to reduce the load on the spindle motor 35.

During the process of S41 to S51, the sample 17A in the sample quantification unit 114 flows from the sample quantification unit 114 to the mixing unit 80 and flows into the mixing unit 80. The specimen 17A merges with the first reagent 18 in the mixing unit 80. The first reagent 18 stored in the reagent receiving unit 305 flows into the mixing unit 80 from the right end 313 of the wall surface 303C. That is, the CPU 91 causes the first reagent 18 overflowing from the partial receiving unit 304 to flow into the mixing unit 80 in the third step. In the fourth step, the sample quantification unit 114 quantifies the sample 17A, and the first reagent 18 once stored in the partial receiving unit 304 is stored in the reagent receiving unit 305. In the fifth step, the sample 17A quantified by the sample quantification unit 114 and the first reagent 18 stored in the reagent receiving unit 305 are caused to flow into the mixing unit 80. As a result, the first reagent 18 and the sample 17A are mixed, and the first mixed liquid 261 is generated. Further, the second reagent 19 moves from the reagent receiving part 342 to the reagent receiving part 343.

<6-6. Sixth step>
The CPU 91 rotates the inspection chip 2 with the rotation speed set to VB, and changes the state of the inspection chip 2 from the rotation angle 0 degree to 90 degrees (S53). The centrifugal direction changes from the direction from the left side 23 to the right side 22 of the test chip 2 shown in FIGS. 2 and 3 to the direction from the upper side 21 to the lower side 24. Thereby, the second reagent 19 starts to flow from the reagent receiving part 343 toward the reagent receiving part 344 side. The second reagent 19 that has flowed to the reagent receiving portion 344 flows into the first connection flow path 301 formed on the front surface 201 via the junction hole portion 351. After the process of S53 is executed, the second reagent 19 is stored in the first connection channel 301 as shown in FIG.

Next, the CPU 91 increases the revolution speed from Vc to Vm (S55). For this reason, centrifugal force becomes large. After increasing the revolution speed to Vm, the CPU 91 holds the revolution speed at Vm (S55). By increasing the revolution speed from Vc to Vm, the CPU 91 causes the second reagent 19 to flow through the first connection channel 301 without remaining in the reagent receiving part 343, the reagent receiving part 344, and the junction hole part 351. be able to.

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S57). For this reason, centrifugal force becomes small. After reducing the revolution speed to Vc, the CPU 91 holds the revolution speed at Vc (S57). When the revolution speed is Vm and the state of the test chip 2 is changed from 90 degrees to 0 degrees in the rotation angle in the process of S59 described later, the spindle motor 35 that supplies a driving force for rotating the turntable 33. The load on is increased. Accordingly, the CPU 91 reduces the revolution speed from Vm to Vc before changing the state of the test chip 2 from 90 degrees to 0 degrees in the process of S59 described later in order to reduce the load on the spindle motor 35.

<6-7. Seventh process>
The CPU 91 rotates the inspection chip 2 with the rotation speed being VB, and changes the state of the inspection chip 2 from the rotation angle of 90 degrees to 0 degrees (S59). The centrifugal direction changes from the direction from the upper side 21 to the lower side 24 of the test chip 2 shown in FIGS. 2 and 3 to the direction from the left side 23 to the right side 22. Thereby, the 2nd reagent 19 in the 1st connection channel 301 begins to flow to the mixing part 80 side. As shown in FIG. 8 (G), the second reagent 19 merges with the first mixed liquid 261. Thereby, the 2nd liquid mixture 262 in which the 1st reagent 18, the 2nd reagent 19, and specimen 17A were mixed is generated.

Next, the CPU 91 increases the revolution speed from Vc to Vm (S61). For this reason, centrifugal force becomes large. After increasing the revolution speed to Vm, the CPU 91 holds the revolution speed at Vm (S61). The CPU 91 can cause the second reagent 19 to flow to the mixing unit 80 without remaining in the first connection channel 301 by increasing the revolution speed from Vc to Vm.

Next, the CPU 91 reduces the revolution speed from Vm to Vc (S63). For this reason, centrifugal force becomes small. CPU91 hold | maintains revolution speed to Vc, after reducing revolution speed to Vc (S63). When the revolution speed is set to Vm, when the state of the inspection chip 2 is changed from 0 degree to 90 degrees in the rotation angle in the process of S65 described later, the spindle motor 35 that supplies a driving force for rotating the turntable 33. The load on is increased. Accordingly, the CPU 91 reduces the revolution speed from Vm to Vc before changing the state of the inspection chip 2 from 0 degree to 90 degrees in the process of S65 described later in order to reduce the load on the spindle motor 35.

After the process of S63 is executed, the second mixed liquid 262 is stored in the mixing unit 80 as shown in FIG. Further, the second mixed liquid 262 is pressed against the wall surface 80 A side of the mixing unit 80 by the action of the centrifugal force X. For this reason, the second mixed liquid 262 spreads in the vertical direction along the wall surface 80A, contacts the entire wall surface 80A, and wets the entire wall surface 80A.

The CPU 91 rotates the inspection chip 2 with the rotation speed set to VB, and changes the state of the inspection chip 2 from the rotation angle 0 degree to 90 degrees (S65). The centrifugal direction changes from the direction from the left side 23 to the right side 22 of the test chip 2 shown in FIGS. 2 and 3 to the direction from the upper side 21 to the lower side 24. When the centrifugal force X acts on the second mixed liquid 262, the second mixed liquid 262 moves from the wall surface 80A to the wall surface 80B side in the mixing unit 80. Thereby, the mixing of the first reagent 18, the second reagent 19, and the specimen 17A in the second mixed liquid 262 is promoted. After the process of S65 is executed, the second mixed liquid 262 is stored on the wall surface 80B side in the mixing unit 80 as shown in FIG. The second mixed liquid 262 is pressed against the wall surface 80B by the action of the centrifugal force X. For this reason, the 2nd liquid mixture 262 contacts the wall surface 80B, and wets the wall surface 80B. The second mixed liquid 262 is brought into a contracted state in contact with the wall surface 80B as shown in FIG. 8 (I) from a state where it spreads along the wall surface 80A as shown in FIG. 8 (H). For this reason, the first reagent 18, the second reagent 19, and the specimen 17A included in the second mixed liquid 262 are further mixed.

Next, the CPU 91 rotates the inspection chip 2 with the rotation speed set to VB, and changes the state of the inspection chip 2 from the rotation angle of 90 degrees to 0 degrees (S67). Since the revolution speed of the test chip 2 is maintained at Vc, the centrifugal direction is from the direction from the upper side 21 to the lower side 24 of the test chip 2 shown in FIGS. 2 and 3 and from the left side 23 to the right side 22. It changes to the direction to go. Thereby, the 2nd liquid mixture 262 moves toward the wall surface 80A side from the wall surface 80B of the mixing part 80. FIG. After the process of S67 is executed, the second mixed liquid 262 is pressed against the wall surface 80A by the action of the centrifugal force X as shown in FIG. The second mixed liquid 262 spreads in the vertical direction along the wall surface 80A and contacts the entire wall surface 80A. The second mixed liquid 262 is brought into a state of expanding along the wall surface 80A as shown in FIG. 9 (J) from the state contracted by contacting the wall surface 80B as shown in FIG. 8 (I). For this reason, the first reagent 18, the second reagent 19, and the specimen 17A included in the second mixed liquid 262 are further mixed.

Next, the CPU 91 decreases the revolution speed from Vc to V0 by stopping the rotation of the spindle motor 35 (S69). Centrifugal force does not act on the second mixed liquid 262 in the mixing unit 80. For this reason, the force acting on the second mixed liquid 262 is only the gravity G in the direction from the upper side portion 21 toward the lower side portion 24. By the action of gravity G, the second mixed liquid 262 moves from the wall surface 80A of the mixing unit 80 toward the wall surface 80B. After the process of S69 is executed, the second mixed liquid 262 is stored on the wall surface 80B side of the mixing unit 80, as shown in FIG.

As shown in FIG. 5, the CPU 91 rotates the turntable 33 to increase the revolution speed from V0 to Ve (S71). As a result, the centrifugal force X in the direction from the left side 23 toward the right side 22 starts to act on the second mixed liquid 262 and gradually increases. Due to the action of the centrifugal force X, the second mixed liquid 262 moves from the wall surface 80B of the mixing unit 80 toward the wall surface 80A. After the process of S71 is executed, the second liquid mixture 262 spreads in the vertical direction along the wall surface 80A of the mixing unit 80 as shown in FIG. Ve that is the revolution speed when the process of S71 is executed is smaller than Vc that is the revolution speed when the process of S63 shown in FIG. 4 is executed. For this reason, the magnitude of the centrifugal force X acting on the second mixed liquid 262 by the process of S71 is smaller than the state after the processes of S63 and S67 shown in FIG. The force pressed against the wall surface 80A is weakened. For this reason, the extent to which the second mixed liquid 262 spreads in the vertical direction along the wall surface 80A is the state after the processing of S63 and S67, that is, the state shown in FIGS. 8 (H) and 9 (J). Smaller than. Accordingly, the region of the wall surface 80A that is in contact with the second mixed liquid 262 does not extend to the entire wall surface 80A, but remains in a part of the wall surface 80A.

Next, the CPU 91 reduces the revolution speed from Ve to V0 by stopping the rotation of the spindle motor 35 (S73). The centrifugal force acting on the second mixed liquid 262 in the mixing unit 80 is eliminated, and only the gravity G in the direction from the upper side 21 to the lower side 24 is obtained. By the action of gravity G, the second mixed liquid 262 moves from the wall surface 80A of the mixing unit 80 toward the wall surface 80B. As a result of the processing of S73 being performed, the second liquid mixture 262 is stored on the wall surface 80B side of the mixing unit 80 as shown in FIG.

Next, the CPU 91 rotates the turntable 33 and increases the revolution speed from V0 to Vc (S75). As a result, the centrifugal force X in the direction from the left side 23 toward the right side 22 starts to act on the second mixed liquid 262 and gradually increases. Next, the CPU 91 reduces the revolution speed from Vc to V0 by stopping the rotation of the spindle motor 35 (S77). The centrifugal force acting on the second mixed liquid 262 is eliminated, and only the gravity G in the direction from the upper side portion 21 to the lower side portion 24 is obtained. Next, the CPU 91 rotates the turntable 33 to increase the revolution speed from V0 to Ve (S79). As a result, the centrifugal force X in the direction from the left side 23 toward the right side 22 starts to act on the second mixed liquid 262 and gradually increases. Next, the CPU 91 decreases the revolution speed from Ve to V0 by stopping the rotation of the spindle motor 35 (S81). The centrifugal force acting on the second mixed liquid 262 is eliminated, and only the gravity G in the direction from the upper side portion 21 to the lower side portion 24 is obtained. The CPU 91 determines whether the processes of S75, S77, S79, and S81 have been repeated a total of 5 times (S83). If the CPU 91 determines that the processes of S75 to S81 have not been repeated a total of 5 times (S83: NO), the process returns to S75. The CPU 91 repeats the processes of S75 to S81 until the processes of S75 to S81 are repeated a total of 5 times.

During the process of S75 to S81, the state of the test chip 2 repeatedly changes to the states shown in FIGS. 9J, 9K, 9L, and 9K. . Accordingly, the second mixed liquid 262 reciprocates between the wall surface 80A and the wall surface 80B of the mixing unit 80. Note that the revolution speed Vc when the process of S75 is executed is different from the revolution speed Ve when the process of S79 is executed. For this reason, the force with which the second mixed liquid 262 is pressed against the wall surface 80A by the action of the centrifugal force X differs between when the process of S75 is executed and when the process of S79 is executed. The amount of movement of the second liquid mixture 262 when the process of S75 is executed is larger than the amount of movement of the second liquid mixture 262 when the process of S79 is executed. Since the second mixed liquid 262 is alternately pressed against the wall surface 80A by the centrifugal force X of different magnitudes, the movement when the second mixed liquid 262 reciprocates between the wall surface 80A and the wall surface 80B in the mixing unit 80. The amount varies alternately.

When the CPU 91 determines that the processes of S75 to S81 have been repeated a total of 5 times (S83: YES), it rotates the turntable 33 and increases the revolution speed from V0 to Ve (S85). As a result, the centrifugal force X in the direction from the left side 23 toward the right side 22 starts to act on the second mixed liquid 262 and gradually increases. Due to the action of the centrifugal force X, the second mixed liquid 262 moves from the wall surface 80B of the mixing unit 80 toward the wall surface 80A. Next, the CPU 91 decreases the revolution speed from Ve to V0 by stopping the rotation of the spindle motor 35 (S87). The centrifugal force acting on the second mixed liquid 262 is eliminated, and only the gravity G in the direction from the upper side portion 21 to the lower side portion 24 is obtained. By the action of gravity G, the second mixed liquid 262 moves from the wall surface 80A of the mixing unit 80 toward the wall surface 80B. Thereby, the second mixed liquid 262 further reciprocates between the wall surface 80A and the wall surface 80B of the mixing unit 80.

<6-8. Eighth process>
The CPU 91 rotates the turntable 33 and increases the revolution speed from V0 to Vm (S89). As a result, the centrifugal force X in the direction from the left side 23 toward the right side 22 starts to act on the second mixed liquid 262 and gradually increases. Thereby, the second mixed liquid 262 is collected on the wall surface 80A side of the mixing unit 80 by the strong centrifugal force X, and is fixed to the wall surface 80A side. Next, the CPU 91 reduces the revolution speed from Vm to Vc (S91). CPU91 hold | maintains revolution speed to Vc, after reducing revolution speed to Vc (S91). When the revolution speed is set to Vm, when the state of the inspection chip 2 is changed from 0 degree to 90 degrees in the rotation angle in the processing of S93 described later, the spindle motor 35 that supplies driving force for rotating the turntable 33. The load on is increased. Therefore, the CPU 91 reduces the revolution speed from Vm to Vc before changing the state of the inspection chip 2 from 0 degree to 90 degrees in the process of S93 described later in order to reduce the load on the spindle motor 35.

The CPU 91 rotates the inspection chip 2 with the rotation speed set to VB, and changes the state of the inspection chip 2 from the rotation angle 0 degree to 90 degrees (S93). The centrifugal direction changes from the direction from the left side 23 to the right side 22 of the test chip 2 shown in FIGS. 2 and 3 to the direction from the upper side 21 to the lower side 24. When the centrifugal force X acts on the second mixed liquid 262, the second mixed liquid 262 moves from the wall surface 80A toward the wall surface 80B along the inner wall surface in the mixing unit 80. Next, the CPU 91 increases the revolution speed from Vc to Vm (S95). For this reason, centrifugal force becomes large. After increasing the revolution speed to Vm, the CPU 91 holds the revolution speed at Vm (S95). By increasing the revolution speed from Vc to Vm, the CPU 91 can cause the second mixed liquid 262 to flow toward the wall surface 80B without causing the liquid mixture to remain on the wall surface 80A side.

CPU91 reduces revolution speed from Vm to Vf (S97). As a result, the centrifugal force X gradually decreases. Next, the CPU 91 reduces the revolution speed from Vf to V0 by stopping the rotation of the spindle motor 35 (S99). The centrifugal force acting on the second liquid mixture 262 is eliminated, and only the gravity G in the direction from the right side portion 22 to the left side portion 23 is obtained. Thereby, the 2nd liquid mixture 262 moves from the wall surface 80B of the mixing part 80 shown in FIG. 2 to the wall surface 80C, and wets the wall surface 80C with the 2nd liquid mixture 262.

Next, the CPU 91 rotates the inspection chip 2 with the rotation speed set to VB, and changes the state of the inspection chip 2 from the rotation angle of 90 degrees to 0 degrees (S101). Gravity G in the direction from the upper side 21 to the lower side 24 acts on the second mixed liquid 262. Thereby, the 2nd liquid mixture 26 moves to the wall surface 80B from the wall surface 80C of the mixing part 80, and is stored by the measurement part 81 shown in FIG.

Next, the CPU 91 rotates the turntable 33 to increase the revolution speed from V0 to Vg (S103). The CPU 91 moves the inspection chip 2 in the steady state to the measurement position. Thereby, it will be in the state in which the test | inspection by allowing measurement light to pass through the measurement part 81 is possible. Next, the CPU 91 decreases the revolution speed from Vg to V0 by stopping the rotation of the spindle motor 35 (S105). The centrifugal force X acting on the second liquid mixture 262 disappears, and only the gravity G in the direction from the upper side portion 21 toward the lower side portion 24 is obtained. The CPU 91 ends the centrifugation process.

When the measurement controller 99 shown in FIG. 1 causes the light source 71 to emit light after the centrifugal process is performed, the measurement light passes through the second mixed liquid 262 stored in the mixing unit 80. The CPU 91 performs optical measurement of the second liquid mixture 262 based on the change amount of the measurement light received by the optical sensor 72, and acquires measurement data. CPU91 calculates the measurement result of the 2nd liquid mixture 262 based on the acquired measurement data. The inspection result of the second mixed liquid 262 based on the measurement result is displayed on the display 96 shown in FIG. In addition, the measuring method of the 2nd liquid mixture 262 is not restricted to an optical measurement, Another method may be sufficient.

<7. Main actions and effects of this embodiment>
As described above, the CPU 91 of the inspection apparatus 1 illustrated in FIG. 1 performs the inspection chip in S71, S75, and S79 illustrated in FIG. 5 in order to mix the reagent 16 and the sample 17 illustrated in FIG. 2 is rotated in the revolution direction, and the rotation is stopped in S69 shown in FIG. 4 and in S73, S77, and S81 shown in FIG. When the sample 17A, the first reagent 18, and the second reagent 19 are mixed without stopping the rotation of the test chip 2, even if the direction of the centrifugal force X is changed, the second direction is perpendicular to the direction of the centrifugal force X. Since the two-mixed liquid 262 spreads, the specimen 17A, the first reagent 18, and the second reagent 19 are difficult to be mixed. On the other hand, after rotating the test chip 2 at the revolution speed Vc in S75 of FIG. 5, the CPU 91 stops the revolution of the test chip 2 in S77 of FIG. Further, after rotating the inspection chip 2 at the revolution speed Ve in S79 of FIG. 5, the CPU 91 stops the revolution of the inspection chip 2 in S81 of FIG. As a result, the second mixed liquid 262 spreading in the direction perpendicular to the direction of the centrifugal force X can spread in a direction perpendicular to the direction of gravity different from the direction of the centrifugal force X. Furthermore, after stopping the rotation of the inspection chip 2 at the revolution speed Vc, the CPU 91 rotates the inspection chip 2 at the revolution speed Ve. As a result, the second mixed liquid 262 spreading in the direction perpendicular to the gravity direction can spread in the direction perpendicular to the direction of the centrifugal force X. Therefore, the specimen 17A, the first reagent 18, and the second reagent 19 can be more uniformly mixed by spreading in the direction orthogonal to the centrifugal force X and the two directions orthogonal to the direction of gravity G.

The CPU 91 sets the revolution speed when the inspection chip 2 is rotated in the revolution direction in S63 shown in FIG. 4 and S75 shown in FIG. 5 to Vc, and the inspection chip 2 is rotated in the revolution direction in S71 and S79 shown in FIG. Let Ve be the revolution speed. In this way, the CPU 91 changes the magnitude of the centrifugal force X acting on the second mixed liquid 262 in the mixing unit 80 by alternately rotating the inspection chip 2 at different revolution speeds. When the centrifugal force X changes, the amount of movement of the second mixed liquid 262 in the mixing unit 80 changes, so that the first reagent 18, the second reagent 19, and the sample 17A included in the second mixed liquid 262 are mixed. Is promoted. Therefore, the CPU 91 can mix the first reagent 18, the second reagent 19, and the sample 17 A included in the second mixed solution 262 more uniformly.

In addition, when the 2nd liquid mixture 262 contacts the wall surfaces 80A and 80B of the mixing part 80 first, the 2nd liquid mixture 262 is easy to be fixed to the wall surfaces 80A and 80B. For this reason, when the 2nd liquid mixture 262 contacts the wall surfaces 80A and 80B again after the 2nd liquid mixture 262 once contacts the wall surfaces 80A and 80B of the mixing part 80, it contacts the wall surfaces 80A and 80B first. The second mixed liquid 262 that comes into contact again is easily slid and moved on the fixed second mixed liquid 262. In this case, if the second mixed liquid 262 simply moves between the wall surface 80A and the wall surface 80B of the mixing unit 80, only the second mixed liquid 262 that slides and moves the immobilized second mixed liquid 262 is the wall surface. Since it becomes easy to shrink by 80B, the 2nd liquid mixture 262 becomes difficult to be mixed uniformly. On the other hand, the CPU 91 includes a step of alternately rotating the inspection chips 2 at different revolution speeds and stopping the revolution of the inspection chips 2. Thereby, it can suppress that the 2nd liquid mixture 262 is fixed to wall

surface

80A, 80B of the mixing part 80. FIG. Therefore, the CPU 91 can mix the first reagent 18, the second reagent 19, and the sample 17 A included in the second mixed solution 262 more uniformly.

CPU91 first rotates the test | inspection chip 2 by Vc which is a relatively high revolution speed by the process of S63 shown in FIG. Accordingly, the second mixed liquid 262 is brought into contact with the entire wall surface 80 A of the mixing unit 80, and the entire wall surface 80 A is moistened with the second mixed liquid 262. Next, CPU91 rotates the test | inspection chip 2 with Ve which is a relatively slow revolution speed by the process of S71 shown in FIG. In this case, the second mixed liquid 262 contacts a part of the wall surface 80A. In this way, the CPU 91 can keep the region of the inner wall surface of the mixing unit 80 in contact with the second mixed solution 262 within the region initially wetted with the second mixed solution 262 by setting the revolution speed to Vc. . The second mixed liquid 262 in contact with the inner wall surface of the mixing unit 80 is easily fixed to the inner wall surface. On the other hand, the CPU 91 prevents the second mixed liquid 262 from protruding outside the entire wall surface 80 A initially wetted by the second mixed liquid 262, so that the second mixed liquid 262 moves in the mixing unit 80. It becomes easy. Accordingly, the CPU 91 moves the second mixed liquid 262 easily in the mixing unit 80, thereby further uniformly mixing the first reagent 18, the second reagent 19, and the sample 17A included in the second mixed liquid 262. Can do.

The CPU 91 causes the first reagent 18, the second reagent 19, and the sample 17A to flow into the mixing unit 80 by the processing up to S63 shown in FIG. 4, and then performs the first reagent by the processing of S71 to S87 shown in FIG. 18, the second reagent 19 and the specimen 17A are mixed. Thereby, CPU91 can mix the 1st reagent 18, the 2nd reagent 19, and the sample 17A uniformly.

<8. Other>
The present invention is not limited to the above embodiment, and various modifications can be made. In the above embodiment, the test chip 2 includes the reagent quantification unit 134A for quantifying the first reagent 18 on the front surface 201 and the reagent quantification unit 134B for quantifying the second reagent 19 on the rear surface 202. The test chip 2 may be configured to include only one reagent quantitative unit 134. In this case, the CPU 91 executes the process of the seventh step after the reagent 16 and the sample 17A quantified by any of the reagent quantification units 134 are flowed into the mixing unit 80, thereby performing the reagent 16 and the sample 17A. Mix.

In the above embodiment, the CPU 91 repeats the processes of S75 to S81 in FIG. The number of repetitions may be 4 times or less, or 6 times or more. The CPU 91 may execute the processes of S75 to S83 in FIG. 5 after the first mixed liquid 261 is generated in the mixing unit 80 by S41 to S51 shown in FIG. 4 and before the process of S53 is executed. . Thereby, the CPU 91 can uniformly mix the first reagent 18 and the sample 17A included in the first mixed liquid 261. As shown in S75 to S81 in FIG. 5, the CPU 91 rotates the inspection chip 2 in the revolution direction by alternately using two different revolution speeds. The CPU 91 may rotate the inspection chip 2 in the revolution direction using three or more different revolution speeds in order.

In the above embodiment, the CPU 91 does not execute the processes of S65 to S69 after flowing the first reagent 18, the second reagent 19, and the sample 17A into the mixing unit 80 by the process up to S63 in FIG. Also good. That is, the CPU 91 causes the first reagent 18, the second reagent 19, and the sample 17A to flow into the mixing unit 80, and then performs the processing of S71 to S83 shown in FIG. 5 without stopping the revolution of the test chip 2. The first reagent 18, the second reagent 19, and the specimen 17A in the second mixed liquid 262 may be mixed.

1 is an example of the “control unit” in the present invention. Vc is an example of the “first rotation speed” in the present invention. Ve is an example of the “second rotational speed” in the present invention.

DESCRIPTION OF SYMBOLS 1 Test | inspection apparatus 2 Test | inspection chip 3 Test | inspection system 16

Reagent

17, 17A, 17B Sample 18 1st reagent 19 2nd reagent 80 Mixing part 90 Control apparatus 91 CPU
114 Sample quantification part 114A First end part 114B Second end part 114C Quantification surface 114D Virtual line 128 Second guide part 134 Reagent quantification part 136 Surplus part 139 Guide part 141 First end part 141A Virtual line 142 Second end part 146 Quantification surface

Claims

A testing device that mixes the sample and the reagent in the mixing unit by rotating a test chip including a mixing unit in which the sample and the reagent are mixed and causing the centrifugal force generated by the rotation to act on the sample and the reagent. Because
Rotating the inspection chip;
After rotating the inspection chip, stop the rotation,
An inspection apparatus comprising: a control unit configured to rotate the inspection chip after stopping the rotation of the inspection chip.
The controller is
Rotating the inspection chip at a first rotation speed;
After rotating the inspection chip at the first rotation speed, stop the rotation,
The inspection apparatus according to claim 1, wherein after the rotation of the inspection chip is stopped, the inspection chip is rotated at a second rotation speed different from the first rotation speed.
The controller is
The inspection apparatus according to claim 2, wherein after the rotation of the inspection chip is stopped, the inspection chip is rotated at the second rotation speed that is slower than the first rotation speed.
The test chip includes a sample quantification unit for quantifying the sample, and a plurality of reagent quantification units for quantifying each of a plurality of reagents,
The controller is
The test chip is rotated after the sample quantified by the sample quantification unit and the plurality of reagents quantified by each of the plurality of reagent quantification units are injected into the mixing unit. The inspection apparatus according to any one of claims 1 to 3.
A test method in which a test chip including a mixing unit in which a sample and a reagent are mixed is rotated, and the sample and the reagent are mixed in the mixing unit by causing a centrifugal force generated by the rotation to act on the sample and the reagent. Because
Rotating the inspection chip;
After rotating the inspection chip, stop the rotation,
An inspection method comprising: a control step of rotating the inspection chip after stopping the rotation of the inspection chip.
A testing device that mixes the sample and the reagent in the mixing unit by rotating a test chip including a mixing unit in which the sample and the reagent are mixed and causing the centrifugal force generated by the rotation to act on the sample and the reagent. On the computer
Rotating the inspection chip;
After rotating the inspection chip, stop the rotation,
An inspection program for executing a control step of rotating the inspection chip after stopping the rotation of the inspection chip.