JP6011156B2 - Inspection chip - Google Patents

Description

  The present invention relates to a test chip for performing a chemical, medical, or biological test of a test object.

  Conventionally, an inspection chip for inspecting biological substances, chemical substances, and the like is known. For example, Patent Document 1 discloses a cassette in which a reagent container containing a reagent can be inserted. A foil cover for sealing the reagent is attached to the surface of the reagent container. When the reagent container is inserted into the cassette, the foil cover is peeled off from the reagent container. Thereby, the reagent accommodated in the reagent container can be supplied to the flow path formed inside the cassette.

JP 2009-92643 A

  However, if the foil cover is peeled off when the reagent container is inserted into the cassette, the reagent that has flowed out of the reagent container may flow out of the cassette. In addition, when the reagent container is inserted into the cassette, the reagent may flow out of the reagent container into the flow path by its own weight before the centrifuge processing of the cassette is performed by the inspection apparatus. At this time, there is a possibility that the reagent flows out into a channel different from the proper channel.

  The objective of this invention is providing the test | inspection chip which reduces a possibility that the reagent accommodated in the reagent container may flow out into the flow path different from an appropriate flow path.

One embodiment of the present invention is a test chip that can accommodate a specimen and a reagent that are liquids inside, and is mixed with the specimen and the reagent by being rotated about a predetermined axis. An insertion port into which a reagent container sealed with a sealing material can be inserted, a reference region in which the reagent container inserted into the insertion port is disposed, and a direction from the reference region toward the insertion port different in the first direction, wherein the reagent container including the container housing portion and the movable space from the reference area, protrude from a direction different from said first direction inside said space, before Symbol reagent containers and A protrusion capable of forming a hole in the sealing material, and protruding from the direction different from the first direction to the inside of the reference region, and when the reagent container is in the reference region, the reagent container Parts different from the sealing material By contacting the, and a regulating portion for regulating said reagent container is moved in a predetermined direction, said first direction, about said axis in a position away from the test chip, the reagent container When the inspection chip in the reference region is rotated, the direction of centrifugal force acting on the reagent container is parallel to the predetermined direction, and the predetermined direction is the rotation of the inspection chip around the axis. The reagent container is parallel to the direction of the Coriolis force acting on the reagent container in the container housing portion, and the reagent container is moved from the reference region by the centrifugal force when the test chip is rotated around the axis. And when the part different from the sealing material moves away from the restriction part in the first direction, the Coriolis force moves in the predetermined direction, and the protrusion part moves from the reference region. Serial in a first direction and said sealing member of said reagent containers to move in the predetermined direction, and forming said hole.

According to the said aspect, the reagent container inserted from the insertion port is arrange | positioned at the reference | standard area | region of a container accommodating part. When the reagent container is moved from the reference region in the first direction within the container housing portion, a hole is formed in the sealing material by the protrusion, and the reagent flows out from this hole. That is, the timing when the reagent flows out from the reagent container is not when the reagent container is inserted from the insertion port, but when the reagent container is moved from the reference region in the first direction. Accordingly, it is possible to reduce the possibility that the reagent stored in the reagent container flows out into a flow path different from the proper flow path. Moreover, it can control that the reagent container accommodated in the container accommodating part moves from a reference | standard area | region other than a desired timing because a control part contacts the site | part different from a sealing material.

The container holder is in said first direction in which the reagent container intersects the second direction is a direction that is inserted into the insertion opening may comprise between pre Kisora. In this case, since the reagent container arranged in the reference region is difficult to return to the insertion port, the possibility of the reagent container jumping out from the insertion port when rotating about a predetermined axis can be reduced.

The protrusion may be provided on the wall surface of the reagent container that faces the seal material moved in the first direction and the predetermined direction from the reference region, among the wall surfaces forming the container housing portion. In this case, the protrusion can reliably come into contact with the reagent container moved in the container housing portion, and a hole through which the reagent flows out can be formed in the sealing material.

The protrusion is provided on a first contact surface that is the wall surface that contacts the reagent container that has moved in the first direction and the predetermined direction from the reference region from the downstream side in the direction of the Coriolis force. Also good. In this case, when the inspection chip is rotated, a hole through which the reagent flows out can be formed in the sealing material of the reagent container that has moved downstream in the direction of the Coriolis force in the container housing portion.

The protrusion of the previous SL length of the second direction may be larger rectangular shape than the length of the first direction. In this case, even when the moving distance of the reagent container in the first direction in the container housing portion is short, the hole extending in the second direction can be formed by the protrusion, so that the fracture surface of the hole can be enlarged.

The protrusions toward the first direction, before Symbol projection height from the first contact surface may be increased. In this case, the reagent container accommodated in the container accommodating portion can be smoothly moved in the first direction.

The protrusion is provided on a second contact surface that is the wall surface that contacts the reagent container that has moved in the first direction and the predetermined direction from the reference region from the downstream side in the centrifugal force direction. Also good . In this case, when the test chip is rotated by centrifugation, a hole through which the reagent flows out can be formed in the seal material of the reagent container moved to the downstream side in the direction of the centrifugal force in the container housing portion.

  A flow path extending in the first direction from the container housing portion may include a reagent supply path through which the reagent that has flowed out of the hole can move. In this case, the reagent flowing out from the hole formed in the sealing material can be moved to the reagent supply path by centrifugal force.

It is a rear view of the inspection apparatus 1 in which the inspection chip 2 is in a steady state. It is a rear view of the test | inspection apparatus 1 in which the test | inspection chip 2 exists in a displacement state. It is a top view of the inspection apparatus 1 shown in FIG. It is a perspective view of the test | inspection chip 2 and the reagent unit 200 based on 1st embodiment. It is a front view of the test | inspection chip 2 before the centrifugation process based on 1st embodiment. It is a top view of the test | inspection chip 2 revolved by the autorotation angle 0 degree based on 1st embodiment. It is a longitudinal cross-sectional view of the test | inspection chip 2 revolved by the autorotation angle 0 degree based on 1st embodiment. FIG. 6 is a front view of the inspection chip 2 revolved at a rotation angle of 0 degree following FIG. 5. FIG. 9 is a front view of the inspection chip 2 revolved at a rotation angle of 90 degrees following FIG. 8. FIG. 10 is a front view of the inspection chip 2 revolved at a rotation angle of 0 degree following FIG. 9. It is a front view of the test | inspection chip 2 after a centrifugation process following FIG. It is a perspective view of the test | inspection chip 2 and the reagent unit 200 based on 2nd embodiment. It is a longitudinal cross-sectional view of the test | inspection chip 2 revolved by the autorotation angle 0 degree based on 2nd embodiment. It is a perspective view of the test | inspection chip 2 and the reagent unit 200 based on 3rd embodiment. It is a longitudinal cross-sectional view of the test | inspection chip 2 revolved by the autorotation angle 0 degree based on 3rd embodiment.

  Embodiments of the present invention will be described with reference to the drawings. The drawings to be referred to are used to explain technical features that can be adopted by the present invention, and are merely illustrative examples.

<1. First embodiment>
A first embodiment of the present invention will be described. A schematic structure of the inspection system 3 will be described with reference to FIGS. 1 and 2. The inspection system 3 of the present embodiment includes an inspection chip 2 that can store a specimen and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2. The inspection apparatus 1 can apply a centrifugal force to the inspection chip 2 by rotation about a vertical axis that is separated from the inspection chip 2. The inspection apparatus 1 can switch the centrifugal direction that is the direction of the centrifugal force applied to the inspection chip 2 by rotating the inspection chip 2 about the horizontal axis.

<1-1. Structure of the inspection apparatus 1>
With reference to FIGS. 1-3, the detailed structure of the inspection apparatus 1 is demonstrated. In the following description, the upper, lower, right, left, front side, and back side of FIGS. 1 and 2 are respectively the upper, lower, right, left, rear, and front sides of the inspection apparatus 1. To do. The upper, lower, right, left, front side, and back side of FIG. 3 are the front, lower, right, left, upper, and lower sides of the inspection apparatus 1, respectively. In the example shown in FIGS. 1 and 2, the direction of the vertical axis is the vertical direction. In the example shown in FIG. 3, the direction of the horizontal axis is the vertical direction. For easy understanding, FIG. 1 and FIG. 2 show the upper housing 30 with a virtual line, and FIG. 3 shows a state where the top plate of the upper housing 30 is removed.

  As shown in FIGS. 1 to 3, the inspection apparatus 1 includes an upper housing 30, a lower housing 31, a turntable 33, an angle changing mechanism 34, a control device 90, and the like. The turntable 33 is a disk-shaped rotating body that is provided on the upper surface side of the lower housing 31 and holds the inspection chip 2 upward. The angle changing mechanism 34 is a drive mechanism that is provided on the turntable 33 and rotates the inspection chip 2 around the horizontal axis. The upper housing 30 is fixed to the upper side of the lower housing 31, and the measurement unit 7 that performs optical measurement on the test chip 2 is provided inside. The control device 90 is a controller that controls centrifugal processing, measurement processing, and the like of the inspection device 1.

  The detailed structure of the lower housing 31 will be described. As shown in FIGS. 1 and 2, the lower housing 31 has a box-like frame structure in which frame members are combined. On the upper surface of the lower housing 31, an upper plate 32 that is a rectangular plate material in plan view is provided. A turntable 33 is rotatably provided above the upper plate 32. A drive mechanism for rotating the turntable 33 around the vertical axis is provided in the lower housing 31 as follows.

  A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side in the lower housing 31. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31 in plan view. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33 in plan view.

  The main shaft 57 is rotatably held by a support member 53 provided directly below the upper plate 32. A pulley 38 is fixed to the main shaft 57 below the support member 53. A belt 39 is stretched over the pulleys 37 and 38. When the main shaft motor 35 rotates the shaft 36, the driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39 and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

  A guide rail 56 extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31. The T-shaped plate 48 is movable in the vertical direction within the lower housing 31 along the guide rail 56. On the front side of the T-shaped plate 48, that is, on the back side in FIG. 1 and FIG. 2, a horizontally long groove 80 is formed in the left-right direction.

  The aforementioned main shaft 57 is a cylindrical body having a hollow inside. The inner shaft 40 is a shaft that can move in the vertical direction within the main shaft 57. An upper end portion of the inner shaft 40 passes through the main shaft 57 and is connected to a rack gear 43 described later. A bearing 41 is provided at the left end of the T-shaped plate 48. Inside the bearing 41, the lower end portion of the inner shaft 40 is rotatably held.

  A stepping motor 51 for moving the T-shaped plate 48 up and down is fixed to the front side of the T-shaped plate 48. The shaft 58 of the stepping motor 51 protrudes toward the rear side, that is, the front side in FIG. 1 and FIG. A disc-shaped cam plate 59 is fixed to the tip of the shaft 58. A cylindrical projection 70 is provided on the rear surface of the cam plate 59. The tip of the protrusion 70 is inserted into the groove 80 described above. The protrusion 70 can slide in the groove 80. When the stepping motor 51 rotates the shaft 58, the projection 70 moves up and down in conjunction with the rotation of the cam plate 59. At this time, the T-shaped plate 48 moves up and down along the guide rail 56 in conjunction with the protrusion 70 inserted in the groove 80.

  The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 fixed to the inner shaft 40 is provided between the pair of L-shaped plates 60. The rack gear 43 is a vertically long metal plate-like member, and gears are carved on both end faces.

  On the front end side in the extending direction of each L-shaped plate 60, a horizontal support shaft 46 having a gear 45 is rotatably supported. Since the support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown), the inspection chip 2 also rotates about the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 rotates about the support shaft 46.

  In the present embodiment, as the main shaft motor 35 rotates and drives the turntable 33, the inspection chip 2 rotates around the main shaft 57, which is a vertical axis, and centrifugal force is applied to the inspection chip 2. The rotation around the vertical axis of the inspection chip 2 is called revolution. On the other hand, as the stepping motor 51 moves the inner shaft 40 up and down, the inspection chip 2 rotates around the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively. . The rotation around the horizontal axis of the inspection chip 2 is called rotation.

  As shown in FIG. 1, when the T-shaped plate 48 is lowered to the lowermost end of the movable range, the rack gear 43 is also lowered to the lowermost end of the movable range. At this time, the inspection chip 2 is in a steady state in which the rotation angle is “0 degree”. As shown in FIG. 2, when the T-shaped plate 48 is raised to the uppermost end of the movable range, the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the inspection chip 2 is rotated from the steady state around the horizontal axis by 180 degrees. That is, in this embodiment, the angle width that the test chip 2 can rotate is the rotation angle of 0 degrees to 180 degrees.

  The detailed structure of the upper housing 30 will be described. As shown in FIG. 3, the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.

  The measurement unit 7 provided in the upper housing 30 includes a light source 71 that emits measurement light and an optical sensor 72 that detects the measurement light emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. In the present embodiment, the left side position of the main shaft 57 in the reciprocable range of the inspection chip 2 is a measurement position at which the inspection chip 2 is irradiated with measurement light. When the inspection chip 2 is at the measurement position, the optical path connecting the light source 71 and the optical sensor 72 intersects the front and rear surfaces of the inspection chip 2 perpendicularly in plan view.

<1-2. Structure of inspection chip 2>
With reference to FIG. 4, the detailed structure of the test | inspection chip 2 and the reagent unit 200 which concern on 1st embodiment is demonstrated. In the following description, the upper, lower, upper right, lower left, lower right, and upper left in FIG. 4 are the upper, lower, right, left, front, and rear of the test chip 2 and the reagent unit 200, respectively. .

  The inspection chip 2 can be mounted with a reagent unit 200 containing a reagent. The reagent unit 200 includes a first container 210, a second container 220, and a connecting body 230. The first container 210 and the second container 220 have a rectangular parallelepiped shape with a small thickness in the front-rear direction and a longitudinal direction in the vertical direction, and hold the first reagent 11 and the second reagent 12 therein, respectively. The connecting body 230 is a rod-like body that is connected to the first container 210 and the second container 220 from above. The first container 210 and the second container 220 are integrally supported by the connecting body 230 side by side in the left-right direction.

  Inside the first container 210, a reagent storage 211 that is a recess opening rearward is provided. The first reagent 11 is sealed inside the reagent storage 211 by a sealing material 212 that closes the opening of the reagent storage 211. An insertion shaft 219 connected to the coupling body 230 is provided at the upper left portion of the first container 210. Similarly, the second container 220 is provided with a reagent storage unit 221 that is a recess opening rearward. The second reagent 12 is sealed inside the reagent storage unit 221 by a sealing material 222 that closes the opening of the reagent storage unit 221. An insertion shaft 229 connected to the coupling body 230 is provided at the upper left portion of the second container 220.

  The inspection chip 2 has a square shape when viewed from the front, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness. On the upper surface of the plate member 20, a sample insertion port 110, a first insertion port 130, and a second insertion port 150 are formed. The sample insertion port 110 is an opening for injecting the sample 10 shown in FIG. For example, the sample 10 accommodated in an instrument (not shown) may be injected into the sample insertion port 110 by a user operation. That is, the sample 10 may be injected into the test chip 2 through the sample insertion port 110 using a known method.

  The first insertion port 130 is an opening for inserting the first container 210 into the inspection chip 2. That is, the first insertion port 130 has a shape into which the first container 210 can be inserted. Specifically, the width in the front-rear direction of the first insertion port 130 is slightly larger than the width in the front-rear direction of the first container 210. The width in the left-right direction of the first insertion port 130 is slightly larger than the width in the left-right direction of the first container 210.

  The second insertion port 150 is an opening for inserting the second container 220 into the inspection chip 2. That is, the second insertion port 150 has a shape into which the second container 220 is inserted. Specifically, the width of the second insertion port 150 in the front-rear direction is slightly larger than the width of the second container 220 in the front-rear direction. The width in the left-right direction of the second insertion port 150 is slightly larger than the width in the left-right direction of the second container 220.

  The sample insertion port 110, the first insertion port 130, and the second insertion port 150 are formed side by side in the left-right direction along the upper side portion 21 that is the upper wall surface of the test chip 2. Of the sample insertion port 110, the first insertion port 130, and the second insertion port 150, the sample insertion port 110 is closest to the left side portion 23, which is the left wall surface of the test chip 2, and the second insertion port 150 is the test chip 2. The first insertion port 130 is located between the sample insertion port 110 and the second insertion port 150, closest to the right side portion 22, which is the right wall surface. The lower wall surface of the inspection chip 2 is the lower side portion 24.

  The front surface of the plate member 20 is sealed with a sheet 29 made of a transparent synthetic resin thin plate. Between the plate member 20 and the sheet 29, a liquid flow path 25 is formed through which the liquid sealed in the inspection chip 2 can flow. The liquid flow path 25 is a recess formed at a predetermined depth on the front side of the plate member 20 and extends in a direction orthogonal to the thickness direction of the plate member 20. That is, the sheet 29 seals the flow path forming surface of the plate material 20. The liquid channel 25 includes a sample storage unit 111, a sample determination unit 114, a sample surplus unit 116, a first container storage unit 140, a reagent determination unit 133, a reagent surplus unit 135, a second container storage unit 160, a reagent determination unit 153, The reagent surplus part 155, the mixing part 170, the storage part 175, etc. are included.

  The sample insertion port 110 communicates with a sample storage unit 111 provided below the sample insertion port 110. The sample storage part 111 is a part in which the sample 10 injected from the sample insertion port 110 is stored, and is a recess that opens upward. A sample supply path 112 extending rightward from the sample storage unit 111 is provided below the sample storage unit 111 and is connected to a sample supply unit 113 having a narrow channel. A sample quantitative unit 114 is provided below the sample supply unit 113. The specimen quantification unit 114 is a part that quantifies the specimen 10, and is a recess that opens upward. By applying a centrifugal force from the sample supply unit 113 toward the inside of the recess of the sample quantification unit 114, the same amount of the sample 10 as the volume inside the recess of the sample quantification unit 114 is quantified.

  A first passage 115 and a second passage 117 extend to the left and right sides from a portion where the sample supply unit 113 and the sample determination unit 114 communicate with each other. The first passage 115 extends to the sample surplus part 116 provided at the lower left of the sample determination unit 114. That is, in the first passage 115, the flow path formation direction changes. The specimen surplus part 116 is a part where the specimen 10 overflowing from the specimen quantification part 114 is stored, and is a concave part extending rightward from the lower end part of the first passage 115. The second passage 117 extends to a later-described mixing unit 170 provided on the right side of the sample determination unit 114.

  The first insertion port 130 communicates with a first container housing part 140 provided on the lower side thereof. The first container housing part 140 includes a reference region 141 in which the first container 210 inserted downward from the first insertion port 130 is disposed. The first container housing part 140 also includes a space in which the first container 210 can move in the right direction from the reference region 141. In other words, the first container housing part 140 also includes an area where the first container 210 can move from the reference area 141 by the action force (centrifugal force, Coriolis) generated during the above revolution.

  A restricting portion 142 is provided in the upper left portion of the first container housing portion 140. The restricting portion 142 has a shape protruding forward toward the inside of the reference region 141. The restricting portion 142 extends in the vertical direction from the upper edge portion of the reference region 141 to the first insertion port 130. In the part where the restricting portion 142 is provided, the width in the front-rear direction of the first insertion port 130 and the reference region 141 is substantially equal to the length in the front-rear direction of the first container 210.

  A protrusion 143 is provided on the right side of the reference region 141 in the first container housing part 140. The protrusion 143 has a rectangular parallelepiped shape in which the length in the vertical direction is larger than the length in the horizontal direction. The protrusion 143 can form a hole through which the first reagent 11 flows out in the sealing material 212 of the first container 210 that moves to the right from the reference region 141. The protruding portion 143 is provided on the first contact surface 145 that is a wall surface forming a part of the rear surface of the first container housing portion 140 among the wall surfaces forming the first container housing portion 140. The first contact surface 145 is a wall surface that the first container 210 that moves to the right from the reference region 141 contacts from the downstream side in the direction of revolution described above.

  The wall surface forming the lower surface of the first container housing part 140 extends horizontally from the reference region 141 in the right direction and bends in the upper right direction at the right end of the first container housing part 140. This bent portion is a restricting portion 144 that restricts the rightward movement of the first container 210. That is, the first container 210 accommodated in the first container accommodating part 140 is movable in the right direction from the reference region 141 to a position where it comes into contact with the restricting part 144.

  A flow path extending in the right direction from the first container housing portion 140 is the reagent supply path 131. In the reagent supply path 131, the first reagent 11 that has flowed out of the hole formed in the sealing material 212 can move. The reagent supply path 131 extends downward via the right side of the restriction part 144, is provided below the first container housing part 140, and is connected to the reagent supply part 132 having a narrow channel. A reagent quantification unit 133 is provided below the reagent supply unit 132. The reagent quantification unit 133 is a part that quantifies the first reagent 11 and is a recess that opens upward. By applying a centrifugal force from the reagent supply unit 132 toward the inside of the concave portion of the reagent quantitative unit 133, the same amount of the first reagent 11 as the volume inside the concave portion of the reagent quantitative unit 133 is quantified.

  A third passage 134 and a fourth passage 136 extend to the left and right sides from a portion where the reagent supply unit 132 and the reagent quantitative unit 133 communicate with each other. The third passage 134 extends to the reagent surplus portion 135 provided on the lower left side of the reagent fixed amount portion 133. That is, in the third passage 134, the formation direction of the flow path is changed. The reagent surplus portion 135 is a portion in which the first reagent 11 overflowing from the reagent quantitative portion 133 is stored, and is a concave portion extending rightward from the lower end portion of the third passage 134. The fourth passage 136 extends to a later-described mixing unit 170 provided on the lower side of the reagent quantitative unit 133.

  The second insertion port 150 communicates with a second container housing portion 160 provided on the lower side thereof. The second container housing portion 160 has the same configuration as the first container housing portion 140, and includes a reference region 161, a restricting portion 162, a protruding portion 163, a restricting portion 164, a first contact surface 165, and the like. A flow path extending rightward from the second container housing portion 160 is a reagent supply path 151. In the reagent supply path 151, the second reagent 12 that has flowed out of the hole formed in the sealing material 222 can move. The reagent supply path 151 extends downward via the right side of the restriction part 164, is provided below the second container housing part 160, and is connected to the reagent supply part 152 formed with a narrow channel. A reagent quantitative unit 153 is provided below the reagent supply unit 152. The reagent quantification unit 153 is a part that quantifies the second reagent 12, and is a recess that opens upward.

  A fifth passage 154 and a sixth passage 156 extend from the site where the reagent supply unit 152 and the reagent quantitative unit 153 communicate with each other to the left and right sides. The fifth passage 154 extends to the reagent surplus portion 155 provided on the lower left side of the reagent fixed amount portion 153. That is, the flow path formation direction of the fifth passage 154 changes. The reagent surplus portion 155 is a portion in which the second reagent 12 overflowing from the reagent quantitative portion 153 is stored, and is a concave portion extending rightward from the lower end portion of the fifth passage 154. By applying a centrifugal force from the reagent supply unit 152 to the inside of the concave portion of the reagent quantitative unit 153, the second reagent 12 having the same volume as the volume inside the concave portion of the reagent quantitative unit 153 is quantified. The sixth passage 156 extends to a later-described mixing unit 170 provided below the reagent quantitative unit 153.

  The mixing unit 170 is a rectangular recess provided in the lower right position of the inspection chip 2 and opening upward in front view. In the mixing unit 170, the specimen 10 flowing from the second passage 117, the first reagent 11 flowing from the fourth passage 136, and the second reagent 12 flowing from the sixth passage 156 are mixed. At least a part of the mixed solution 13 of the specimen 10, the first reagent 11, and the second reagent 12 is stored in a storage unit 175 that is a recess provided in the lower right portion of the mixing unit 170.

As shown in FIG. 1, the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates counterclockwise around the support shaft 46 in a front view. When the inspection chip 2 is in the steady state shown in FIG. 5, the upper side 21 and the lower side 24 are orthogonal to the gravity direction Z, the right side 22 and the left side 23 are parallel to the gravity direction Z, and the left side 23 is the right side. It is arranged closer to the main shaft 57 than the portion 22. When the inspection chip 2 is disposed at the measurement position in a steady state, the optical path connecting the light source 71 and the optical sensor 72 passes through the storage unit 175 vertically in a plan view.
<1-3. Example of inspection method>

  An inspection method using the inspection apparatus 1 and the inspection chip 2 will be described with reference to FIGS. In order to facilitate understanding, FIGS. 5 and 8 to 11 show the front view of the test chip 2 with the sheet 29 removed. In FIGS. 5 to 11, the protrusions 143 and 163 are color-coded by hatched hatching. FIG. 7A is a cross-sectional view taken along the line I-I in FIG. FIG. 7B is a cross-sectional view taken along the line II-II in FIG. FIG. 7C is a cross-sectional view taken along the line III-III in FIG.

  First, the user attaches the test chip 2 to the support shaft 46 and attaches the reagent unit 200 to the test chip 2. At this time, as shown in FIGS. 5, 6 (A), and 7 (A), when the first container 210 is inserted from the first insertion port 130, the first container 210 moves downward along the restricting portion 142, Arranged in the reference region 141. In the reference region 141, the restricting portion 142 contacts the rear surface of the insertion shaft 219 on the upper side of the sealing material 212. The first container 210 disposed in the reference region 141 is sandwiched between the front and rear sides by the seat 29 and the restricting portion 142.

  Similarly, when the second container 220 is inserted from the second insertion port 150, the second container 220 moves downward along the restricting portion 162 and is disposed in the reference region 161. In the reference region 161, the restricting portion 162 contacts the rear surface of the insertion shaft 229 on the upper side of the sealing material 222. The second container 220 disposed in the reference region 161 is sandwiched between the front and rear sides by the seat 29 and the restricting portion 162.

  Further, the user injects the specimen 10 from the specimen insertion port 110. The injected sample 10 is stored in the sample storage unit 111. Thereafter, when the user inputs a process start command to the inspection apparatus 1, the following measurement operation is executed. Note that the inspection apparatus 1 can process two inspection chips 2 at the same time, but for the convenience of explanation, a procedure for inspecting using one inspection chip 2 will be described below.

  First, by the drive control of the spindle motor 35, the inspection chip 2 having a rotation angle of 0 degrees is revolved. At this time, as shown in FIG. 6A and FIG. 8, the centrifugal force X acts on the test chip 2 toward the downstream side in the centrifugal direction. In the present embodiment, when the test chip 2 has a rotation angle of 0 degree, the centrifugal force X works from the left side portion 23 toward the right side portion 22. As shown in FIG. 6B, the first container 210 moves to the right by the centrifugal force X until it comes into contact with the restricting portion 144 from the reference region 141. The second container 220 is moved by the centrifugal force X from the reference region 161 to a position where it comes into contact with the restricting portion 164.

  When the inspection chip 2 revolves, the Coriolis force Y acts on the inspection chip 2 toward the downstream side in the revolution direction. In the present embodiment, as shown in FIG. 7A, a Coriolis force Y works from the front side to the rear side of the test chip 2. In a state where the restricting portion 142 is in contact with the insertion shaft 219, the backward movement of the first container 210 is restricted even if the Coriolis force Y is applied. Similarly, when the restricting portion 162 is in contact with the insertion shaft 229, the backward movement of the second container 220 is restricted even if the Coriolis force Y is applied. On the other hand, as shown in FIGS. 6B and 7B, when the first container 210 is moved a predetermined distance from the reference region 141 to the right side, the restricting portions 142 and 162 are separated from the insertion shafts 219 and 229, respectively. The first container 210 and the second container 220 can move rearward.

  Accordingly, as shown in FIGS. 6C, 7 </ b> C, and 8, the first container 210 moves backward to the position where it contacts the first contact surface 145 by the Coriolis force Y. At this time, the protrusion 143 contacts the sealing material 212 to form the hole R. The hole R has a vertically long fracture surface whose length in the vertical direction is larger than the length in the horizontal direction, corresponding to the vertically long shape of the protrusion 143 described above. The first reagent 11 held in the reagent storage unit 211 flows out of the formed hole R, moves to the right by the centrifugal force X, and flows into the reagent supply path 131.

  Similarly, when the second container 220 moves rearward by the Coriolis force Y, the protrusion 163 contacts the seal material 222 to form the hole R. The second reagent 12 held in the reagent storage unit 221 flows out of the formed hole R, moves to the right by the centrifugal force X, and flows into the reagent supply path 151. At the same time, the sample 10 stored in the sample storage unit 111 flows into the sample supply path 112 by the centrifugal force X.

  Next, by the drive control of the stepping motor 51, the revolving inspection chip 2 is rotated 90 degrees counterclockwise when viewed from the front, and the rotation angle is displaced to "90 degrees". Thereby, as shown in FIG. 9, the centrifugal force X acts on the test chip 2 from the upper side portion 21 toward the lower side portion 24. The sample 10 that has flowed into the sample supply path 112 by the action of the centrifugal force X flows into the sample quantification unit 114 via the sample supply unit 113. In the sample quantification unit 114, the sample 10 exceeding a predetermined amount overflows into the first passage 115 and is stored in the sample surplus unit 116, whereby the predetermined amount of the sample 10 is quantified.

  At the same time, the first reagent 11 that has flowed into the reagent supply path 131 by the action of the centrifugal force X flows into the reagent quantitative unit 133 through the reagent supply unit 132. In the reagent quantification unit 133, the first reagent 11 exceeding a predetermined amount overflows into the third passage 134 and is stored in the reagent surplus unit 135, whereby the predetermined amount of the first reagent 11 is quantified. Similarly, the second reagent 12 that has flowed into the reagent supply path 151 by the action of the centrifugal force X flows into the reagent quantitative unit 153 via the reagent supply unit 152. In the reagent quantification unit 153, the second reagent 12 exceeding a predetermined amount overflows into the fifth passage 154 and is stored in the reagent surplus unit 155, whereby the predetermined amount of the second reagent 12 is quantified.

  Next, by the drive control of the stepping motor 51, the revolving inspection chip 2 is rotated 90 degrees clockwise in front view and displaced to a steady state. Thereby, as shown in FIG. 10, the centrifugal force X acts on the test chip 2 from the left side portion 23 toward the right side portion 22. The direction of the centrifugal force X is controlled so that the specimen 10 stored in the specimen surplus section 116 does not move to the specimen quantitative section 114 again. That is, since the specimen surplus portion 116 is a concave portion that closes in the right direction, the surplus specimen 10 remains without moving from the specimen surplus portion 116. On the other hand, the sample 10 quantified by the sample quantification unit 114 moves to the right by the action of the centrifugal force X, and flows into the mixing unit 170 via the second passage 117.

  At the same time, the first reagent 11 quantified by the reagent quantification unit 133 flows into the mixing unit 170 through the fourth passage 136 by the action of the centrifugal force X. On the other hand, since the reagent surplus portion 135 is a recess that closes in the right direction, the surplus first reagent 11 remains in the reagent surplus portion 135. Similarly, the second reagent 12 quantified by the reagent quantification unit 153 flows into the mixing unit 170 through the sixth passage 156 by the action of the centrifugal force X. On the other hand, since the reagent surplus portion 155 is a recess that closes in the right direction, the surplus second reagent 12 remains in the reagent surplus portion 155. The specimen 10, the first reagent 11, and the second reagent 12 that have flowed into the mixing unit 170 are mixed by the action of the centrifugal force X, and the mixed liquid 13 is generated.

  Finally, the inspection chip 2 is moved to the measurement position by drive control of the spindle motor 35, and the revolution operation of the inspection chip 2 is completed. As shown in FIG. 11, a part of the mixed liquid 13 generated in the mixing unit 170 moves in the gravity direction Z and is stored in the storage unit 175. The mixed liquid 13 is optically measured by the optical path passing through the storage unit 175. The inspection apparatus 1 obtains the inspection result of the specimen 10 based on the attenuation amount of the measurement light received by the optical sensor 72. The test result of the specimen 10 is displayed on a display (not shown), for example.

<1-4. Action and effect of this embodiment>
As described above, according to the inspection chip 2 of the first embodiment, the first container 210 inserted from the first insertion port 130 is disposed in the reference region 141 of the first container housing part 140. When the first container 210 is moved rightward from the reference region 141 in the first container housing part 140, a hole R is formed in the sealing material 212 by the protrusion 143, and the first reagent 11 flows out from the hole R. .

  That is, the timing when the first reagent 11 flows out of the first container 210 is not when the first container 210 is inserted from the first insertion port 130 but when the first container 210 is moved rightward from the reference region 141. It is. The first reagent 11 is supplied from the first container 210 to the liquid flow path 25 after the start of the centrifugal process without being supplied from the first container 210 to the liquid flow path 25 before the start of the centrifugal process. Therefore, it is possible to suppress the first reagent 11 from flowing out from the first container 210 before the start of the centrifugal treatment, and as a result, it is possible to reduce the possibility that the first reagent 11 flows into a flow path different from the proper flow path.

  The first container housing part 140 includes a space in which the first container 210 can move from the reference region 141 in the right direction intersecting the downward direction in which the first container 210 is inserted into the first insertion port 130. Therefore, the first container 210 arranged in the reference region 141 is less likely to return to the first insertion port 130, so that the risk of the first container 210 jumping out of the first insertion port 130 during revolution can be reduced.

  In a state where the first container 210 is disposed in the reference region 141, the restricting portion 142 contacts the insertion shaft 219 that is a part different from the sealing material 212. For example, when an external force that moves the first container 210 in the right direction acts and the external force is smaller than the centrifugal force X, the first container 210 can be prevented from moving in the right direction from the reference region 141. At this time, since the restricting portion 142 does not contact the sealing material 212, the movement of the first container 210 can be restricted without being damaged by the restricting portion 142. Therefore, it can suppress that the 1st container 210 moves from the reference | standard area | region 141 except a desired timing.

  The protrusion 143 is provided on the first contact surface 145 that faces the sealing material 212 of the first container 210 that moves to the right from the reference region 141 among the wall surfaces that form the first container housing 140. Therefore, the protrusion 143 can be surely brought into contact with the first container 210 that moves in the first container housing portion 140, and the hole R through which the first reagent 11 flows out can be formed in the sealing material 212. Furthermore, the first contact surface 145 is a wall surface that the first container 210 that moves to the right from the reference region 141 contacts from the downstream side in the direction of rotation about the main shaft 57. Therefore, when the inspection chip 2 is centrifugally rotated, the hole R can be formed in the sealing material 212 of the first container 210 that moves to the downstream side in the direction of rotation in the first container housing portion 140.

  The protrusion 143 has a rectangular parallelepiped shape in which the length in the vertical direction is larger than the length in the horizontal direction. Therefore, even when the rightward movement distance of the first container 210 in the first container housing portion 140 is short, the hole R extending in the vertical direction is formed by the protrusion 143 to increase the fracture surface of the hole R. Can do.

  The reagent supply path 131 is a flow path extending in the right direction from the first container housing portion 140. Therefore, the first reagent 11 flowing out from the hole R can be moved to the reagent supply path 131 by centrifugal force without changing the centrifugal direction. The operations and effects described above are the same for the second container 220 inserted from the second insertion port 150 and the second reagent 12 accommodated in the second container 220.

<2. Second embodiment>
A second embodiment of the present invention will be described. Below, the same code | symbol as 1st embodiment is attached | subjected to the same structure as 1st embodiment, description is abbreviate | omitted, and only a different point from 1st embodiment is demonstrated.

  With reference to FIG. 12, the detailed structure of the test | inspection chip 2 and the reagent unit 200 which concern on 2nd embodiment is demonstrated. The reagent unit 200 is the same as that of the first embodiment (see FIG. 4). On the other hand, as shown in FIG. 12, the inspection chip 2 of the present embodiment is not provided with the restriction portions 142 and 162. For this reason, as shown in FIG. 13A, the first container 210 in which the reference region 141 is arranged is slightly movable in the front-rear direction. The second container 220 disposed in the reference region 161 is slightly movable in the front-rear direction.

  In the inspection chip 2 of the present embodiment, protrusions 146 and 166 are provided instead of the protrusions 143 and 163 of the first embodiment. The protrusions 146 and 166 are provided on the first contact surfaces 145 and 165, respectively. The stepped portion has a triangular prism shape extending in the up-down direction and has a length in the up-down direction larger than a length in the left-right direction. Each of the protrusions 146 and 166 has an inclined surface whose height from the first contact surface 145 increases in the right direction. However, the inclination angle of the protrusion 166 with respect to the first contact surface 145 is larger than the inclination angle of the protrusion 146 with respect to the first contact surface 145.

  With reference to FIG. 13, the test | inspection method using the test | inspection chip 2 of 2nd embodiment is demonstrated. When the measurement operation is executed by the inspection apparatus 1, the inspection chip 2 in a steady state is revolved as in the first embodiment. As shown in FIG. 13A, the Coriolis force Y acts from the front side to the rear side of the inspection chip 2, and the centrifugal force X acts from the left side 23 to the right side 22. Accordingly, as shown in FIG. 13B, the first container 210 moves rightward along the first contact surface 145 by the centrifugal force X and the Coriolis force Y. At this time, the first container 210 is slightly inclined forward and right along the inclined surface of the protrusion 146. Similarly, the second container 220 moves to the right along the first contact surface 165 by the centrifugal force X and the Coriolis force Y.

  When the first container 210 moves from the reference region 141 to the right by a predetermined distance, the tip of the protrusion 146 contacts the sealing material 212. Since the first container 210 is urged rearward by the Coriolis force Y, the protruding portion 146 breaks the sealing material 212 to form the hole R as shown in FIG. Similarly, when the second container 220 moves from the reference region 161 to the right by a predetermined distance, the tip of the protrusion 166 comes into contact with the sealing material 222. Since the second container 220 is urged rearward by the Coriolis force Y, the protrusion 166 breaks the seal material 222 to form the hole R. As a result, the first reagent 11 and the second reagent 12 of the reagent unit 200 flow into the reagent supply paths 131 and 151, respectively. The subsequent processing is the same as in the first embodiment.

  As described above, according to the test chip 2 of the second embodiment, the first reagent 11 can be prevented from flowing out of the first container 210 before the start of the centrifugal treatment, as in the first embodiment, and thus The possibility that the first reagent 11 flows out into a channel different from the proper channel can be reduced. Furthermore, the protrusions 146 and 166 have inclined surfaces whose height from the first contact surface 145 increases toward the right. Therefore, the first container 210 accommodated in the first container accommodating part 140 can be smoothly moved rightward. Similarly, the 2nd container 220 accommodated in the 2nd container accommodating part 160 can be moved to right direction smoothly.

<3. Third Embodiment>
A third embodiment of the present invention will be described. Below, the same code | symbol as 1st, 2nd embodiment is attached | subjected to the same structure as 1st, 2nd embodiment, description is abbreviate | omitted, and only a different point from 1st, 2nd embodiment is demonstrated. .

  With reference to FIG. 14, the detailed structure of the test | inspection chip 2 and the reagent unit 200 which concern on 3rd embodiment is demonstrated. In the reagent unit 200 of the present embodiment, the reagent storage units 211 and 221 are concave portions that open in the right direction. Therefore, the sealing materials 212 and 222 are also provided on the right side of the reagent storage units 211 and 221.

  In the inspection chip 2 of the present embodiment, the front surface side of the plate material 20 is sealed with a sheet 29, and the rear surface side of the plate material 20 is sealed with a sheet 28. The inspection chip 2 of the present embodiment is not provided with the restriction portions 142 and 162. For this reason, as shown in FIG. 15A, the first container 210 in which the reference region 141 is arranged is slightly movable in the front-rear direction. The second container 220 disposed in the reference region 161 is slightly movable in the front-rear direction.

  In the first container housing part 140, a gap part 149 is provided between the reference region 141 and the restriction part 144. Similarly, in the second container housing portion 160, a gap portion 169 is provided between the reference region 161 and the restriction portion 164. The gaps 149 and 169 are portions where the plate member 20 is cut out in the vertical direction, and penetrate the plate member 20 in the front-rear direction. The widths of the gaps 149 and 169 in the left-right direction are equal to or slightly larger than the lengths of the insertion shafts 219 and 229 in the left-right direction, respectively.

  The inspection chip 2 of this embodiment is provided with protrusions 147 and 167 instead of the protrusions 143 and 163 of the first embodiment. The protruding portion 147 is provided slightly behind the second contact surface 148 that is the upper surface of the limiting portion 144. The second contact surface 148 is a wall surface that the first container 210 that moves to the right from the reference region 141 contacts from the downstream side in the direction of the centrifugal force generated when rotating around the main shaft 57 described above. Similarly, the protruding portion 167 is provided on the second contact surface 168 that is the upper surface of the limiting portion 164. The protrusions 147 and 167 have a conical shape protruding toward the left side.

  With reference to FIG. 15, the test | inspection method using the test | inspection chip 2 of 3rd embodiment is demonstrated. When the measurement operation is executed by the inspection apparatus 1, the inspection chip 2 in a steady state is revolved as in the first embodiment. As shown in FIG. 15A, the Coriolis force Y acts from the front side to the rear side of the inspection chip 2, and the centrifugal force X acts from the left side 23 to the right side 22. Thereby, as shown in FIG. 15B, the first container 210 moves to the right along the wall surface forming the rear surface of the first container housing portion 140 by the centrifugal force X and the Coriolis force Y. Similarly, the second container 220 moves to the right along the wall surface forming the rear surface of the second container housing portion 160 by the centrifugal force X and the Coriolis force Y.

  When the first container 210 moves a predetermined distance in the right direction from the reference region 141, the distal end portion of the protrusion 147 comes into contact with the sealing material 212 and the insertion shaft 219 moves to the gap portion 149. Since the first container 210 is urged rearward by the Coriolis force Y, the insertion shaft 219 moves rearward along the gap 149 to a position where it comes into contact with the seat 28 as shown in FIG. At this time, the protruding portion 147 breaks the sealing material 212 in the front-rear direction to form the hole R.

  Similarly, when the second container 220 moves to the right from the reference region 161 by a predetermined distance, the tip of the protrusion 167 comes into contact with the seal material 222 and the insertion shaft 229 moves to the gap 169. Since the second container 220 is urged rearward by the Coriolis force Y, the insertion shaft 229 moves rearward along the gap 169 to a position where it comes into contact with the seat 28. At this time, the protrusion 167 breaks the sealing material 222 in the front-rear direction to form the hole R. As a result, the first reagent 11 and the second reagent 12 of the reagent unit 200 flow into the reagent supply paths 131 and 151, respectively. The subsequent processing is the same as in the first embodiment.

  As described above, according to the test chip 2 of the third embodiment, the first reagent 11 can be prevented from flowing out of the first container 210 before the start of the centrifugal treatment, as in the first embodiment, and consequently The possibility that the first reagent 11 flows out into a channel different from the proper channel can be reduced. The protruding portion 147 is provided on the second contact surface 148 facing the seal material 212 of the first container 210 that moves to the right from the reference region 141 among the wall surfaces forming the first container housing portion 140. The second contact surface 148 is a wall surface that the first container 210 that moves to the right from the reference region 141 contacts from the downstream side in the direction of the centrifugal force generated when the main vessel 57 rotates around the main shaft 57. Therefore, when the inspection chip 2 is rotated by centrifugation, the hole R can be formed in the sealing material 212 of the first container 210 that moves to the downstream side in the direction of the centrifugal force in the first container housing portion 140. Similarly, a hole R through which the second reagent 12 flows out can be formed in the sealing material 222 by the protrusion 167 provided on the second contact surface 148.

<4. Other>
In the above embodiment, the first reagent 11 and the second reagent 12 correspond to the “reagent” of the present invention. The first container 210 and the second container 220 correspond to the “reagent container” of the present invention. The first insertion port 130 and the second insertion port 150 correspond to the “insertion port” of the present invention. The first container housing portion 140 and the second container housing portion 160 correspond to the “container housing portion” of the present invention. The right direction corresponds to the “first direction” of the present invention. The downward direction corresponds to the “second direction” of the present invention.

  The present invention is not limited to the above embodiment, and various modifications can be made. The inspection device 1 and the inspection chip 2 of the above embodiment are merely examples, and the structure, shape, processing, and the like of each can be changed.

  In the above embodiment, the reagent unit 200 in which the first container 210 and the second container 220 are connected is illustrated in consideration of user convenience, but the first container 210 and the second container 220 are used alone. Also good. In the above embodiment, the rightward movement of the first container 210 is restricted by the restricting portion 144, but the right side of the first container 210 is brought into contact with the right edge of the first insertion port 130 by the insertion shaft 219. Directional movement may be restricted. Similarly, the rightward movement of the second container 220 may be restricted by the insertion shaft 229 coming into contact with the right edge of the second insertion port 150. That is, the right edge portion of the first insertion port 130 or the right edge portion of the second insertion port 150 is an example of the restriction portion of the present invention.

  The “first direction” and the “second direction” are not limited to the right direction and the downward direction, and may be directions that are not opposite to each other. For example, the sample insertion port 110, the first insertion port 130, and the second insertion port 150 are provided in order from the top on the left side portion 23 instead of the upper side portion 21 of the test chip 2. The user inserts the first container 210 into the first insertion port 130 from the left side of the inspection chip 2. In this case, before the centrifugal force X is applied, the first container 210 is held in the reference region 141 by the restricting portion 142. When the centrifugal force X is applied, the first container 210 moves rightward from the reference region 141, the sealing material 212 is broken by the protrusion 143, and the hole R through which the first reagent 11 flows can be formed. is there. In this case, when the user inserts the first container 210 into the test chip 2, if a strong force is applied in the insertion direction, the first reagent 210 is inserted before the centrifugal force X is applied. May leak. Therefore, the above-described embodiment is preferable.

  The “projection” may be changed in quantity, shape, position, size, and the like within a range in which a hole can be formed in the reagent container that moves in the first direction from the reference region. The number of “reagents” injected into the test chip 2 is not limited to two, and may be one reagent or three or more reagents. In the above embodiment, the inspection chip 2 includes the plate material 20 and the sheets 28 and 29, but the inspection chip 2 may not include the sheets 28 and 29. For example, you may use the test | inspection chip 2 in which the various insertion ports and the liquid flow path 25 were directly formed in the board | plate material 20. FIG.

2 Test chip 10 Specimen 11 First reagent 12 Second reagent 57 Main shaft 130 First insertion port 131 Reagent supply path 140 First container housing part 141 Reference region 142 Restriction part 143 Projection part 145 First contact surface 146 Projection part 147 Projection part 148 Second contact surface 150 Second insertion port 151 Reagent supply path 160 Second container accommodating portion 161 Reference region 162 Restriction portion 163 Protrusion portion 165 First contact surface 166 Protrusion portion 167 Protrusion portion 168 Second contact surface 200 Reagent unit 210 First One container 212 Sealing material 220 Second container 222 Sealing material

Claims (8)

It is a test chip that can accommodate a liquid sample and reagent inside and mixes the sample and the reagent by being rotated around a predetermined axis,
An insertion port into which a reagent container in which the reagent contained inside is sealed with a sealing material can be inserted;
A reference region in which the reagent container inserted into the insertion port is disposed, and a space in which the reagent container is movable from the reference region in a first direction different from a direction from the reference region toward the insertion port. and including the container housing portion,
And different projects from inwardly of the space, the possible forming a hole in the sealing member protrusion before Symbol reagent containers from said first direction,
The reagent protrudes from the direction different from the first direction to the inside of the reference region, and when the reagent container is in the reference region, the reagent container is brought into contact with a portion different from the sealing material, A regulation unit that regulates movement of the container in a predetermined direction ,
The first direction is a centrifugal force acting on the reagent container when the inspection chip is rotated with the reagent container in the reference region around the axis located at a position away from the inspection chip. Parallel to the direction,
The predetermined direction is parallel to the direction of the Coriolis force acting on the reagent container in the container housing portion when the inspection chip rotates around the axis,
The reagent container moves in the first direction from the reference region by the centrifugal force when the inspection chip rotates around the axis, and a portion different from the sealing material is moved from the restricting portion to the first portion. When moving away in the direction, the Coriolis force moves in the predetermined direction,
The inspection chip , wherein the protrusion forms the hole in the sealing material of the reagent container that has moved in the first direction and the predetermined direction from the reference region . The container accommodating unit, the first direction crossing the second direction in which the reagent container is inserted into the insertion port, the inspection chip according to claim 1, characterized in that it comprises between pre Kisora. The protrusion is provided on the wall surface of the reagent container that faces the sealing material of the reagent container that has moved in the first direction and the predetermined direction from the reference region among the wall surfaces forming the container housing portion. The inspection chip according to claim 1 or 2 . The protrusions with respect to the reagent container has been moved to said first direction and said predetermined direction from said reference region, provided in the first contact surface is the wall that contacts the downstream side in the direction of the Coriolis force The inspection chip according to claim 3 . The protrusions test chip of claim 2, before Symbol length of the second direction, wherein the first direction is larger rectangular parallelepiped shape than the length. The protrusions test chip of claim 4 toward the first direction, before Symbol projection height from the first contact surface and said increase. The protrusion is provided to the reagent container has been moved to said first direction and said predetermined direction from said reference region, the second contact surface is the wall that contacts the downstream side in the direction of the front Kito centering forces The inspection chip according to claim 3 , wherein: The flow channel extending in the first direction from the container holder, according to any one of claims 1 to 7, wherein the reagent flowing out of the hole with a reagent supply channel movable Inspection chip.

Images