JP5137528B2 - Analytical device, analytical apparatus and analytical method using the same - Google Patents

Description

  The present invention relates to an analytical device used for analyzing a liquid collected from a living organism, an analytical apparatus and an analytical method using the analytical device, and more particularly to diluting a sample liquid in the analytical device. The present invention relates to a technique for opening a diluent container holding the diluent.

  Conventionally, as a method for analyzing a liquid collected from a living organism or the like, a method for analyzing using a device for analysis in which a liquid channel is formed is known. The analytical device can control the fluid using a rotating device, and utilizes centrifugal force to dilute the sample liquid, measure the solution, separate the solid component, transfer and distribute the separated fluid, Since a solution and a reagent can be mixed, various biochemical analyzes can be performed.

  In particular, dilution of the sample solution is a necessary step for analyzing a very small amount of sample, but it is an operation for the user to inject a necessary amount of the dilution solution from the outside into the analytical device every time measurement is performed. Due to the poor performance, a configuration in which a diluent container in which a diluent is previously held in an analytical device is accommodated and can be easily opened has been studied.

  In the analytical device described in Patent Document 1 that performs analysis by diluting the sample liquid, as shown in FIG. 17A, a diluent container 51 is accommodated in the chamber 50 in the analytical device. Diluent container 51 has a thin film seal 52 and a rigid side 54 with a marking mark 53. The diluent container 51 is held at a predetermined position by a holding post 55. FIG. 17B shows a state in which the spindle or post 56 has entered the chamber 50 through the receiving hole 57. In this position, the post 56 receives the diluent container 51 and moves it toward the chamber 58, and the rigid side 54 is broken along the scribing mark 53 to create an opening 59. The diluent held in the diluent container 51 flows out by the rotation of the rotor and is transferred into the receiving chamber 58 through the outlet channel 60. The receiving chamber 58 is a mixing chamber in which the sample liquid and the diluent are mixed.

Further, as shown in FIG. 18, the analysis device described in Patent Document 2 has a configuration in which a liquid supply / reservoir 62 is accommodated in the analysis device main body 61 and is held in the liquid supply / reservoir 62. The liquid reagent 63 is introduced into the reaction path 66 by pulling the end 65 of the membrane 64. Thereafter, the liquid reagent 63 freely flows into the corner 68 of the reaction path 66 by gravity along the path indicated by the dashed arrow 67. The sample is taken in by introducing the capillary holder 69 into the analysis device main body 61. 70, 71, 72 are reagents.
JP 7-503794 gazette Japanese Patent Laid-Open No. 3-46566

  However, in Patent Document 1, in order to make the diluent container 51 easy to break, the thickness of the container at the portion of the marking mark 53 is reduced, and the moisture permeability of the diluent container 51 is increased. Further, since it is necessary to use a rigid resin member for the thin film seal 52, the moisture permeability of the seal portion is increased, and the diluted solution in the diluted solution container 51 cannot be stored for a long period of time. The measurement accuracy is affected by fluctuations in reagent and reagent concentration. Furthermore, there is a problem that the mechanism for operating the post 55 is required in the apparatus, the configuration becomes complicated, and the cost of the analyzer increases.

  Further, in Patent Document 2, as work performed by the user before setting in the analyzer, a work of collecting a sample solution with the sample collecting capillary 73 of the capillary holder 69, and the capillary holder 69 in the analysis device main body 61 are provided. Since there are an operation to introduce and an operation to pull the film 64 of the liquid supply / reservoir 62, the operability is bad for the user and there is a risk of erroneous operation. Furthermore, since the film 64 must be peeled off and discarded, there is a problem that the amount of dust increases and becomes a burden on the user.

  The present invention solves the conventional problems, and uses an analytical device that can store a diluted solution for a long period of time and can open the diluted solution with a simple operation without complicating the structure of the analyzer. An object is to provide an analysis apparatus and an analysis method.

  The analysis device according to claim 1 of the present invention has a microchannel structure for transferring a sample solution toward a measurement spot by centrifugal force, and is used for reading to access a reaction solution at the measurement spot. The analysis device main body having a microchannel structure having a fine concavo-convex shape on the surface formed therein, and the inlet for collecting the sample liquid in the microchannel structure is exposed in the open position, and the base substrate in the closed position. A protective cap that covers a part of the cover substrate and prevents scattering of the sample liquid from the inlet, a diluent container whose opening is sealed with a seal member so as to hold the diluent therein, and A diluent container storage section formed inside the analysis device body and movably storing the diluent container between a liquid holding position and a liquid discharge position; Opening the diluent container by breaking the seal member of the diluent container that is provided in the container accommodating portion so as to protrude in the movement path from the liquid holding position to the liquid discharge position of the diluent container and moved to the liquid discharge position. Provided with a protrusion, and configured to move the dilution liquid container to the open position where the seal member engages and breaks the protrusion by opening and closing the protective cap from the open position to the closed position. The dilution liquid container is prevented from moving to the liquid discharge position by directly or indirectly engaging the liquid container and a part of the protective cap in the closed position before the inlet is exposed in the open position. The diluent container is configured to be locked at the liquid holding position.

  The analytical device according to claim 2 of the present invention is the analytical device according to claim 1, wherein the diluent container is provided with a latch portion on the side of the protective cap, and the inlet is exposed to the open position with the diluent container. The protective cap at the previous closed position engages with the latch portion of the diluent container, and the diluent container is locked at the liquid holding position of the diluent container housing portion. When the protective cap is released from the open position by closing the protective cap from the open position to the closed position, the protective cap is opened by exposing the inlet to the open position against the engagement with the protective cap. Is configured to contact the surface of the latch portion of the diluent container on the side of the protective cap and push the diluent container into the liquid discharge position.

  The analysis device according to claim 3 of the present invention is characterized in that, in claim 1 or claim 2, the seal surface to which the seal member of the diluent container is attached is formed obliquely.

  The analysis device according to claim 4 of the present invention is the analysis device according to claim 2, wherein the analysis device main body is provided with a hole through which a locking jig protrudes into the movement path, and is formed on the upper surface or the lower surface of the diluent container. Is characterized in that a groove for locking the diluent container by engaging the locking jig through the hole at the liquid holding position is provided.

  The analysis device according to claim 5 of the present invention is characterized in that, in claim 2, the diluent container is formed with an arc surface on the opposite side of the open portion sealed by the seal member. To do.

  According to a sixth aspect of the present invention, there is provided an analytical apparatus in which the analytical device according to the first aspect in which a sample liquid is sampled is set, wherein the analytical device is rotated and stopped around an axis. Rotating drive means for transferring the sample liquid and the diluent discharged from the diluent container to the measurement chamber, and analysis means for accessing and analyzing the solution in the measurement chamber.

  According to a seventh aspect of the present invention, there is provided an analytical method using the analytical device according to the first aspect, wherein the sample liquid is spotted on the inlet that is exposed by opening the protective cap of the analytical device. The diluent container set in the diluent container container of the analytical device by the operation from the open position of the protective cap to the closed position is removed from the diluent container in the diluent container container. Pushing the seal member of the diluent container into the projecting part by pushing toward the projecting part provided projecting into the movement path from the liquid holding position to the liquid discharge position, and opening the diluent container; The analysis device opened by breaking the seal member is set in a rotor having an axial center, and at least a part of the sample liquid spotted on the analysis device is rotated by rotating the rotor. A step of diluting with a diluent released from the diluent container, and a step of accessing and analyzing the solution component diluted with the diluent or the reaction product of the solution component diluted with the diluent and the reagent. Features.

  According to the analysis device of the present invention and the analysis apparatus and analysis method using the analysis device, the user opens the diluent container with a minimum operation for collecting the sample solution, and automatically places the dilution solution in the analysis device. Since it can be transferred, the analysis accuracy can be improved, the analyzer can be simplified, the cost can be reduced, and the operability for the user can also be improved.

Hereinafter, embodiments of an analysis device, an analysis apparatus using the analysis device, and an analysis method according to the present invention will be described with reference to FIGS.
1 to 6 show an analytical device.

  FIGS. 1A and 1B show a state in which the protective cap 2 of the analysis device 1 is closed and opened. FIG. 2 shows an exploded state with the lower side in FIG. 1 (a) facing upward, and FIG. 3 shows an assembly drawing thereof.

  As shown in FIG. 1 and FIG. 2, this analytical device 1 includes a base substrate 3 having a microchannel structure having a fine concavo-convex shape on one surface, a cover substrate 4 covering the surface of the base substrate 3, It is composed of four parts including a diluent container 5 holding a diluent and a protective cap 2 for preventing sample liquid scattering.

The base substrate 3 and the cover substrate 4 are joined with the diluent container 5 and the like set therein, and the protective cap 2 is attached to the joined substrate.
By covering the openings of several concave portions formed on the upper surface of the base substrate 3 with the cover substrate 4, a plurality of storage areas described later (same as measurement spots described later) and the microchannels connecting the storage areas are connected. A flow path having a structure is formed. Necessary ones of the storage areas are loaded with reagents necessary for various analyses. One side of the protective cap 2 is pivotally supported so that it can be opened and closed by engaging with the shafts 6 a and 6 b formed on the base substrate 3 and the cover substrate 4. When the sample liquid to be examined is blood, the gap between the flow paths of the microchannel structure on which the capillary force acts is set to 50 μm to 300 μm.

  The outline of the analysis process using this analytical device 1 is that the sample solution is spotted on the analytical device 1 in which a diluent is set in advance, and at least a part of the sample solution is diluted with the diluent, and then measurement is performed. It is what.

FIG. 4 shows the shape of the diluent container 5.
4A is a plan view, FIG. 4B is a cross-sectional view taken along line AA of FIG. 4A, FIG. 4C is a side view, FIG. 4D is a rear view, and FIG. These are front views seen from the opening 7. The opening 7 is sealed with an aluminum seal 9 as a seal member after filling the interior 5a of the diluent container 5 with the diluent 8 as shown in FIG. 6 (a). On the opposite side of the diluent container 5 from the opening 7, a latch portion 10 is formed. The dilution liquid container 5 is formed between the base substrate 3 and the cover substrate 4 and is set in the dilution liquid container housing part 11 so that the liquid holding position shown in FIG. 6A and the liquid discharge shown in FIG. It is housed in a freely movable position.

FIG. 5 shows the shape of the protective cap 2.
5 (a) is a plan view, FIG. 5 (b) is a cross-sectional view taken along line BB of FIG. 5 (a), FIG. 5 (c) is a side view, FIG. 5 (d) is a rear view, and FIG. These are the front views seen from the opening 2a. As shown in FIG. 6 (a) in the closed state shown in FIG. 1 (a), a locking groove 12 with which the latch portion 10 of the diluent container 5 can be engaged is formed inside the protective cap 2. ing.

  FIG. 6A shows the analysis device 1 before use. In this state, the protective cap 2 is closed, and the latch portion 10 of the diluent container 5 is engaged with the locking groove 12 of the protective cap 2 so as to prevent the diluent container 5 from moving in the arrow J direction. Locked in position. In this state, it is supplied to the user.

  When the protective cap 2 is opened as shown in FIG. 1B against the engagement with the latch portion 10 in FIG. 6A when the sample liquid is spotted, the locking groove of the protective cap 2 As shown in FIG. 6 (b), the bottom portion 2b formed with 12 is elastically deformed, and the engagement between the locking groove 12 of the protective cap 2 and the latch portion 10 of the diluent container 5 is released.

  In this state, the sample solution is spotted on the exposed inlet 13 of the analytical device 1 and the protective cap 2 is closed. At this time, by closing the protective cap 2, the wall surface 14 that has formed the locking groove 12 comes into contact with the surface 5 b of the latch portion 10 of the diluent container 5 on the side of the protective cap 2, and the diluent container 5 is pushed in the direction of arrow J (direction approaching the liquid discharge position). An opening rib 14 as a protruding portion is formed in the diluent container housing portion 11 from the base substrate 3 side, and when the diluent container 5 is pushed in by the protective cap 2, the diluent container 5 is inclined obliquely. The aluminum seal 9 stretched on the sealing surface of the opening 7 collides with the opening rib 14 and is broken as shown in FIG.

As shown in FIGS. 7 and 8, the analysis device 1 is set on the rotor 101 of the analysis apparatus 100 with the cover substrate 4 facing downward, so that the component analysis of the sample solution can be performed.
A groove 102 is formed on the upper surface of the rotor 101. When the analysis device 1 is set on the rotor 101, the groove 102 is formed on the rotation support 15 and the protective cap 2 formed on the cover substrate 4 of the analysis device 1. The rotation support portion 16 engages with and accommodates the groove 102.

  When the analysis device 1 is set on the rotor 101 and the door 103 of the analyzer is closed before the rotor 101 is rotated, the set analysis device 1 is moved by the movable piece 104 provided on the door 103 side. The position on the rotational axis of the rotor 101 is pressed against the rotor 101 by the biasing force of the spring 105, and the analysis device 1 rotates integrally with the rotor 101 that is rotationally driven by the rotational driving means 106. Reference numeral 107 denotes an axial center during rotation of the rotor 101. The protective cap 2 is attached in order to prevent the sample liquid adhering to the vicinity of the inlet 13 from being scattered outside due to centrifugal force during analysis.

  As a material of the parts constituting the analysis device 1, a resin material having a low material cost and excellent mass productivity is desirable. Since the analysis apparatus 100 analyzes the sample liquid by an optical measurement method for measuring light transmitted through the analysis device 1, the material of the base substrate 3 and the cover substrate 4 may be PC, PMMA, AS, MS, or the like. A synthetic resin having high transparency is desirable.

  Further, as the material of the diluent container 5, since it is necessary to enclose the diluent 8 in the diluent container 5 for a long period of time, a crystalline synthetic resin having a low moisture permeability such as PP and PE is desirable. As a material for the protective cap 2, there is no particular problem as long as it is a material with good moldability, and an inexpensive resin such as PP or PE is desirable.

  The base substrate 3 and the cover substrate 4 are preferably joined by a method that hardly affects the reaction activity of the reagent carried in the storage area. Ultrasonic welding or laser welding is less likely to generate reactive gas or solvent during joining. Etc. are desirable.

  Further, a hydrophilic treatment for increasing the capillary force is applied to the portion where the solution is transferred by the capillary force due to the minute gap between the substrates 3 and 4 by joining the base substrate 3 and the cover substrate 4. Specifically, hydrophilic treatment using a hydrophilic polymer or a surfactant is performed. Here, hydrophilic means that the contact angle with water is less than 90 °, more preferably less than 40 °.

FIG. 9 shows the configuration of the analyzer 100.
The analysis apparatus 100 includes a rotation drive unit 106 for rotating the rotor 101, an optical measurement unit 108 for optically measuring the solution in the analysis device 1, and the rotation speed and direction of the rotor 101 and optical. Control means 109 for controlling the measurement timing and the like of the measurement means, a calculation unit 110 for processing the signal obtained by the optical measurement means 108 and calculating the measurement result, and for displaying the result obtained by the calculation unit 110 Display unit 111.

  The rotation driving means 106 not only rotates the analyzing device 1 around the rotation axis 107 at a predetermined rotation speed around the rotation axis 107 via the rotor 101 but also centers on the rotation axis 107 at a predetermined stop position. The analyzing device 1 can be swung by reciprocating left and right in a predetermined amplitude range and cycle.

  The optical measurement means 108 includes a laser light source 112 for irradiating the measurement unit of the analysis device 1 with laser light, and the amount of transmitted light that has passed through the analysis device 1 out of the laser light emitted from the laser light source 105. And a photo detector 113 for detecting.

  The analysis device 1 is driven to rotate by the rotor 101, and the sample liquid taken into the inside from the injection port 13 is rotated around the rotation axis 107 located on the inner periphery of the injection port 13. The centrifugal force generated and the capillary force of the capillary channel provided in the analysis device 1 are used to transfer the solution inside the analysis device 1. The channel structure will be described in detail along with the analysis process.

FIG. 10 shows the vicinity of the inlet 13 of the analytical device 1.
FIG. 10A shows an enlarged view of the injection port 13 as seen from the outside of the analytical device 1, and FIG. 10B shows the microchannel structure seen through the cover substrate 4 from the rotor 101 side. It is.

  The inlet 13 is a gap where a capillary force acts like the guiding part 17 via a guiding part 17 where a capillary force acts in a minute gap δ formed between the base substrate 3 and the cover substrate 4. A capillary cavity 19 having a volume capable of holding a required amount of sample liquid 18 is connected. The cross-sectional shape (DD cross section in FIG. 10B) perpendicular to the flow direction of the guide portion 17 is not a rectangular shape with the back side vertical, but as shown in FIG. It forms in the inclined surface 20 which becomes narrow gradually toward. The guide portion 17, the capillary cavity 19, and the connection portion are formed with a bent portion 22 that forms a recess 21 in the base substrate 3 and changes the direction of the passage.

  A gap separation cavity 23 where a capillary force does not act is formed at the tip of the guide cavity 17 through the capillary cavity 19. A cavity 24 having one end connected to the separation cavity 23 and the other end open to the atmosphere is formed on a side of a part of the capillary cavity 19, the bent portion 22, and the guide portion 17.

  With this configuration, when spotted on the inlet 13, the sample liquid 18 is taken up to the capillary cavity 19 via the guide portion 17. FIG. 11 shows a state before the analysis device 1 after spotting is set on the rotor 101 and rotated. At this time, as explained in FIG. 6C, the aluminum seal 9 of the diluent container 5 collides with the opening rib 14 and is broken. Reference numerals 25 a, 25 b, 25 c, and 25 d are air holes formed in the base substrate 3.

− Step 1 −
As shown in FIG. 12A, the analysis device 1 holds the sample solution in the capillary cavity 19 and is set on the rotor 101 in a state where the aluminum seal 9 of the diluted solution 5 is broken.

− Step 2 −
When the rotor 101 is rotationally driven in the clockwise direction (C2 direction) after the door 103 is closed, the held sample solution is broken at the position of the bent portion 22, and the sample solution in the guide portion 17 is discharged into the protective cap 2. Then, the sample liquid 18 in the capillary cavity 19 flows into the separation cavity 23 as shown in FIGS. 12B and 15A, and is centrifuged into the plasma component 18a and the blood cell component 18b in the separation cavity 23. The The diluent 8 that has flowed out of the diluent container 5 flows into the holding cavity 27 via the discharge channels 26a and 26b. When the dilution liquid 8 flowing into the holding cavity 27 exceeds a predetermined amount, the excess dilution liquid 8 flows into the overflow cavity 29 via the overflow channel 28 and further passes through the rib 30 for backflow prevention to the reference measurement chamber. It flows into 31.

  The diluent container 5 has a bottom portion opposite to the opening 7 sealed with the aluminum seal 9 and is formed with an arcuate surface 32 as shown in FIGS. 4 (a) and 4 (b). At the liquid discharge position of the state diluent container 5 shown in 12 (b), as shown in FIG. 13, the center m of the arc surface 32 is offset by a distance d so as to be closer to the discharge channel 26b side than the rotation axis 107. Therefore, the diluent 8 that has flowed toward the arc surface 32 is changed to a flow (in the direction of arrow n) from the outside toward the opening 7 along the arc surface 32, and the diluent container 5 is changed. Are efficiently discharged from the opening 7 to the diluent container housing 11.

− Step 3 −
Next, when the rotation of the rotor 101 is stopped, the plasma component 18 a is sucked up into the capillary cavity 33 formed on the wall surface of the separation cavity 23, and is passed through the capillary channel 37 communicating with the capillary cavity 33 as shown in FIG. As shown in FIG. 15B, the fixed amount flows through the metering flow path 38 and is held. FIG. 15C shows a perspective view of the capillary cavity 33 and its periphery.

− Step 4 −
When the rotor 101 is rotationally driven in the counterclockwise direction (C1 direction), as shown in FIG. 14B, the plasma component 18a held in the measuring flow path 38 is measured through the backflow preventing rib 39 to the measurement chamber 40. In addition, the diluent 8 in the holding cavity 27 flows into the measurement chamber 40 through the siphon-shaped connection channel 41 and the backflow prevention rib 39. Further, the sample liquid in the separation cavity 23 flows into the overflow cavity 36 via the siphon-shaped connection channel 34 and the rib 35 for backflow prevention. Then, as necessary, the rotor 101 is reciprocally rotated counterclockwise (C1 direction) and clockwise (C2 direction) and is swung to comprise the reagent, the diluent 8 and the plasma component 18a carried in the measurement chamber. Stir the solution to be measured.

− Step 5 −
When the rotor 101 is rotated counterclockwise (C1 direction) or clockwise (C2 direction) and the measurement spot of the reference measurement chamber 31 passes between the laser light source 112 and the photodetector 113, the calculation unit 110 detects the photodetector 113. The detected value is read to determine the reference value. Further, at the timing when the measurement spot of the measurement chamber 40 passes between the laser light source 112 and the photodetector 113, the calculation unit 110 reads the detection value of the photodetector 113 and calculates a specific component based on the reference value.

  As described above, since the diluent container 5 can be opened by opening and closing the protective cap 2 when the user collects the sample solution, and the diluent can be transferred into the analysis device 1, the analyzer can be simplified. The cost can be reduced, and the operability for the user can be improved.

  Further, the diluent container 5 sealed with the aluminum seal 9 as the sealing member is used, and the diluent container 5 is opened by breaking the aluminum seal 9 with the opening rib 14 as the protruding portion. The analysis accuracy can be improved without the dilution liquid evaporating and decreasing.

  6A, the latch portion 10 of the diluent container 5 is engaged with the locking groove 12 of the closed protective cap 2, and the diluent container 5 is engaged. Is held at the liquid holding position so that it does not move in the direction of arrow J, so that the diluent container 5 is configured to be movable in the diluent container housing portion 11 by opening and closing the protective cap 2. During the period until the user opens the protective cap 2 and uses it, the position of the diluent container 5 in the diluent container housing 11 is locked to the liquid holding position, so that the user can transport the container before use. There is no case where the diluent container 5 is accidentally opened and the diluent is spilled.

  FIG. 16 shows a manufacturing process in which the analyzing device 1 is set to the shipping state shown in FIG. First, before closing the protective cap 2, the groove 42 (see FIGS. 2B and 4D) provided on the lower surface of the diluent container 5 and the hole 43 provided in the cover substrate 4 are aligned. At this liquid holding position, the protrusion 44a of the locking jig 44 provided separately from the base substrate 3 or the cover substrate 4 is engaged with the groove 42 of the diluent container 5 through the hole 43, so that the diluent container 5 is engaged. Is set in a state of being locked at the liquid holding position. Then, the protective cap 2 is closed in a state where the pressing jig 46 is inserted from the notch 45 (see FIG. 1) formed on the upper surface of the protective cap 2 and the bottom surface of the protective cap 2 is pressed and elastically deformed. Can be set to the state shown in FIG. 6A by releasing the pressing jig 46.

  In this embodiment, the case where the groove 42 is provided on the lower surface of the diluent container 5 has been described as an example. However, the groove 42 is provided on the upper surface of the diluent container 5, and the base substrate corresponds to the groove 42. 3 can be configured such that a hole 43 is provided in the groove 3 and the protrusion 44 a of the locking jig 44 is engaged with the groove 42.

  In the above embodiment, the locking groove 12 of the protective cap 2 is directly engaged with the latch portion 10 of the diluent container 5 to lock the diluent container 5 in the liquid holding position. It is also possible to indirectly engage the locking groove 12 and the latch portion 10 of the diluent container 5 to lock the diluent container 5 at the liquid holding position.

  In the above embodiment, the analysis device 1 is rotated around the rotation axis 107 to transfer the components centrifuged from the sample solution and the diluent 8 released from the diluent container 5 to the measurement chamber 40. Although the case where the solution component separated from the sample solution or the reaction product of the solution component separated from the sample solution and the reagent is analyzed is described as an example, the solution component is separated from the sample solution. If it is not necessary, the centrifugation step is not necessary. In this case, the analytical device 1 is rotated around the rotation axis 107, and a fixed amount of the sample solution is spotted. The entire solution and the diluted solution 8 released from the diluted solution container 5 are transferred to the measurement chamber 40 for dilution, and the solution component diluted with the diluted solution or the reaction product of the solution component diluted with the diluted solution and the reagent. Access and analyze.

  Further, the analysis device 1 is rotated around the rotation axis 107 and the solid component separated from the sample solution and the diluted solution released from the diluent container 5 are transferred to the measurement chamber for dilution and separated from the sample solution. It is also possible to access and analyze the reaction product of the solid component separated from the sample solution or the solid component and the reagent.

  In the above embodiment, the analysis device body in which the microchannel structure having a fine uneven shape on the surface is formed in the two layers of the base substrate 3 and the cover substrate 4, but the substrate of three or more layers Can also be configured. Specifically, a three-layer structure that forms a microchannel structure by centering a substrate having a notch formed in accordance with the microchannel structure and bonding another substrate on the upper and lower surfaces thereof to close the notch. The structure can be given as an example.

  The present invention is useful as a transfer control means for an analytical device used for component analysis of a liquid collected from a living organism.

FIG. 3 is an external perspective view of the analytical device according to the embodiment of the present invention with the protective cap closed and opened. Exploded perspective view of the analysis device of the same embodiment A perspective view of the analytical device with the protective cap closed as seen from the back Explanatory drawing of the diluent container of the embodiment Explanatory drawing of the protective cap of the embodiment Sectional drawing of the state in which the protective cap is closed before using the analytical device of the same embodiment, when spotting the sample liquid, and after spotting Perspective view just before setting the analysis device to the analyzer Sectional view of the analysis device set in the analyzer Configuration diagram of the analyzer of the same embodiment Expansion explanatory drawing of the principal part of the analysis device of the embodiment Sectional view before setting the analysis device to the analyzer and starting rotation Sectional view after setting the analytical device in the analyzer and rotating and then centrifuging Sectional drawing which shows the position of the diluent container at the timing when the diluent is released from the rotation axis of the device for analysis and the diluent container Sectional view when the solid component of the sample solution after centrifugation is quantitatively collected and diluted Enlarged view and perspective view of main parts Cross-sectional view of the process to set to the shipping state Plan views before and after opening the diluent container in the analytical device of the conventional example Sectional view when opening the diluent container in another conventional analytical device

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Analytical device 2 Protective cap 2a Opening 2b Bottom part 3 Base substrate 4 Cover substrate 5 Diluent container 5a Inside 5b Surface of latch part 10 6a, 6b Shaft 7 Opening 8 Diluent 9 Aluminum seal (seal member)
DESCRIPTION OF SYMBOLS 10 Latch part 11 Dilution liquid container accommodating part 12 Groove for locking 13 Inlet 14 Opening rib (protrusion part)
DESCRIPTION OF SYMBOLS 15,16 Rotation support part 17 Guide part 18 Sample liquid 18a Plasma component 18b Blood cell component 19 Capillary cavity 20 Inclined surface 21 Recessed part 22 Bending part 23 Separation cavity 24 Cavity 25a, 25b, 25c, 25d Air hole 26a, 26b Exhaust flow path 27 Retaining cavity 28 Overflow channel 29 Overflow cavity 30 Rib 31 Reference measurement chamber 32 Arc surface 33 Capillary cavity 34 Connection channel 35 Rib 36 Overflow cavity 37 Capillary channel 38 Metering channel 39 Backflow prevention rib 40 Measurement Chamber 41 Connection channel 42 Groove 43 Hole 44 Locking jig 44a Protrusion 45 Notch 46 Pressing jig 100 Analyzing device 101 Rotor 102 Groove 103 Door 104 Movable piece 105 Spring 106 Rotation drive means 107 Rotating shaft 108 Optical measuring hand Stage 109 Control means 110 Arithmetic unit 111 Display unit 112 Laser light source 113 Photo detector

Claims (7)

An analytical device having a microchannel structure for transferring a sample liquid toward a measurement spot by centrifugal force, and used for reading to access a reaction liquid in the measurement spot,
An analysis device body in which a microchannel structure having a fine uneven shape on the surface is formed;
A protective cap that covers a part of the base substrate and the cover substrate and prevents scattering of the sample liquid from the injection port in the open position, exposing an inlet for collecting the sample liquid to the microchannel structure in the open position;
A diluent container whose opening is sealed with a sealing member so as to hold the diluent therein;
A diluent container storage section that is formed inside the analysis device main body and movably stores the diluent container between a liquid holding position and a liquid discharge position;
The diluent container that breaks the seal member of the diluent container that protrudes in the movement path from the liquid holding position of the diluent container to the liquid discharge position in the diluent container housing portion and moves to the liquid discharge position. A projecting portion for opening the cover, and opening and closing the protective cap from the open position to the closed position to move the diluent container to the open position where the seal member engages and breaks the projecting portion. ,
The diluent container does not move to the liquid discharge position due to direct or indirect engagement with the diluent container and a part of the protective cap in the closed position before the inlet is exposed in the open position. An analytical device configured to lock the diluent container at the liquid holding position. The diluent container is provided with a latch portion on the side of the protective cap,
The protective cap in the closed position before opening the inlet with the diluent container is engaged with the latch portion of the diluent container, so that the diluent container is the liquid in the diluent container storage portion. Locked in the holding position,
The engagement is released by opening the protective cap against the engagement between the latch portion of the diluent container and the protective cap to expose the inlet and closing the protective cap from the open position. The analytical device according to claim 1, wherein when closing to the position, the protective cap is configured to abut against the surface on the side of the protective cap of the latch portion of the diluent container to push the diluent container into the liquid discharge position. . The analysis device according to claim 1, wherein a sealing surface to which the sealing member of the diluent container is attached is formed obliquely. The analysis device body is provided with a hole for projecting a locking jig into the movement path,
The analysis device according to claim 2, wherein a groove for locking the diluent container by engaging the locking jig through the hole at a liquid holding position is provided on an upper surface or a lower surface of the diluent container. The analysis device according to claim 2, wherein the diluent container is formed with an arcuate bottom portion on the opposite side of the open portion sealed by the seal member. An analysis apparatus in which the analysis device according to claim 1 that has collected a sample liquid is set,
Rotation drive means for rotating and stopping the analytical device around an axis to transfer the sample liquid and the diluted liquid released from the diluent container to the measurement chamber;
And an analysis device for accessing and analyzing the solution in the measurement chamber. An analysis method using the analysis device according to claim 1,
Open the protective cap of the analytical device and collect the sample solution by spotting it on the exposed inlet.
The diluent container set in the diluent container container of the analytical device by the operation from the open position of the protective cap to the closed position is moved from the liquid holding position of the diluent container in the diluent container container. Pushing toward a protrusion provided projecting into the movement path to the discharge position, pressing the seal member of the diluent container against the protrusion and opening the diluent container;
The analysis device opened by breaking the seal member is set in a rotor having an axial center, and at least a part of the sample liquid spotted on the analysis device is rotated from the dilution liquid container by rotating the rotor. Diluting with the released diluent; and
And a step of accessing and analyzing the solution component diluted with the diluent or the reaction product of the solution component diluted with the diluent and the reagent.

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